Abstract：
:The E. coli replication fork synthesizes DNA at the rate of nearly 1000 nt/s. We show here that an interaction between the tau subunit of the replicative polymerase (the DNA polymerase III holoenzyme) and the replication fork DNA helicase (DnaB) is required to mediate this high rate of replication fork movement. In the absence of this interaction, the polymerase follows behind the helicase at a rate equal to the slow (approximately 35 nt/s) unwinding rate of the helicase alone, whereas upon establishing a tau-DnaB contact, DnaB becomes a more effective helicase, increasing its translocation rate by more than 10-fold. This finding establishes the existence of both a physical and communications link between the two major replication machines in the replisome: the DNA polymerase and the primosome.
journal_name
Celljournal_title
Cellauthors
Kim S,Dallmann HG,McHenry CS,Marians KJdoi
10.1016/s0092-8674(00)81039-9subject
Has Abstractpub_date
1996-02-23 00:00:00pages
643-50issue
4eissn
0092-8674issn
1097-4172pii
S0092-8674(00)81039-9journal_volume
84pub_type
杂志文章相关文献
CELL文献大全abstract：:TOR complex 1 (TORC1) is a potent anabolic regulator of cellular growth and metabolism. When cells have sufficient amino acids, TORC1 is active due to its lysosomal localization mediated via the Rag GTPases. Upon amino acid removal, the Rag GTPases release TORC1, causing it to become cytoplasmic and inactive. We show ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.01.024
更新日期：2014-02-13 00:00:00
abstract：:Enveloped viruses enter cells by inducing fusion of viral and cellular membranes, a process catalyzed by a specialized membrane-fusion protein expressed on their surface. This review focuses on recent structural studies of viral fusion proteins with an emphasis on their metastable prefusion form and on interactions wi...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2018.02.054
更新日期：2018-03-08 00:00:00
abstract：:Xenopus laevis oocytes respond to high temperature (greater than 31 degrees C) by the synthesis of one major (70 kilodalton) protein and by a gradual reduction in the rate of normal protein synthesis. In contrast with most other cells, the heat-shock response of Xenopus oocytes is controlled exclusively at the transla...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(82)90443-3
更新日期：1982-07-01 00:00:00
abstract：:Although clonal neo-antigen burden is associated with improved response to immune therapy, the functional basis for this remains unclear. Here we study this question in a novel controlled mouse melanoma model that enables us to explore the effects of intra-tumor heterogeneity (ITH) on tumor aggressiveness and immunity...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.08.032
更新日期：2019-09-19 00:00:00
abstract：:To determine the special feature of ribosomal RNA promoters that might account for the highly efficient and regulated synthesis of rRNA in E. coli, we have analyzed the beginnings of two ribosomal RNA operons, rrnD and rrnX. DNA sequences for 425 bp preceding those specifying mature 16s rRNA are reported. In vitro tra...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(79)90310-6
更新日期：1979-05-01 00:00:00
abstract：:During development, sensory axons compete for limiting neurotrophic support, and local neurotrophin insufficiency triggers caspase-dependent axon degeneration. The signaling driving axon degeneration upon local deprivation is proposed to reside within axons. Our results instead support a model in which, despite the ap...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.01.032
更新日期：2016-02-25 00:00:00
abstract：:Mitochondrial DNA replication was examined in mutants for seven different Saccharomyces cerevisiae genes which are essential for nuclear DNA replication. In cdc8 and cdc21, mutants defective in continued replication during the S phase of the cell cycle, mitochondrial DNA replication ceases at the nonpermissive tempera...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(75)90061-6
更新日期：1975-08-01 00:00:00
abstract：:We have constructed a gene fusion using the promoter of Drosophila hsp70 and the structural gene for Drosophila alcohol dehydrogenase (Adh) and used this construct to transform Adh-deficient flies. In these transformants, Adh is expressed only after heat shock. Like hsp70 itself, this heat-shock-inducible Adh (Adhhs) ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(84)90432-x
更新日期：1984-07-01 00:00:00
abstract：:Plasma membrane vesicles and protoplasts of Escherichia coli were fused to planar lipid bilayers and studied with electrophysiological techniques. Large transmembrane aqueous channels were opened when 0.2 nM LamB signal peptide was added to the cytoplasmic side of the membrane. These aqueous pores are similar in condu...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90231-z
更新日期：1992-05-15 00:00:00
abstract：:Tissue homeostasis requires maintenance of functional integrity under stress. A central source of stress is mechanical force that acts on cells, their nuclei, and chromatin, but how the genome is protected against mechanical stress is unclear. We show that mechanical stretch deforms the nucleus, which cells initially ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.03.052
更新日期：2020-05-14 00:00:00
abstract：:Frequent codirectional collisions between the replisome and RNA polymerase (RNAP) are inevitable because the rate of replication is much faster than that of transcription. Here we show that, in E. coli, the outcome of such collisions depends on the productive state of transcription elongation complexes (ECs). Codirect...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2011.07.034
更新日期：2011-08-19 00:00:00
abstract：:A strong transcription enhancer was identified in the genomic DNA (235 kb) of human cytomegalovirus (HCMV), a ubiquitous and severe pathogen of the herpesvirus group. Cotransfection of enhancerless SV40 DNA with randomly fragmented HCMV DNA yielded two SV40-HCMV recombinant viruses that had incorporated overlapping se...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(85)80025-8
更新日期：1985-06-01 00:00:00
abstract：:Neuroinflammation is being increasingly recognized as a potential mediator of cognitive impairments in various neurological conditions. Habbas et al. demonstrate that the pro-inflammatory cytokine tumor necrosis factor alpha signals through astrocytes to alter synaptic transmission and impair cognition in a mouse mode...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2015.12.001
更新日期：2015-12-17 00:00:00
abstract：:We describe a new repressible binary expression system based on the regulatory genes from the Neurospora qa gene cluster. This "Q system" offers attractive features for transgene expression in Drosophila and mammalian cells: low basal expression in the absence of the transcriptional activator QF, high QF-induced expre...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2010.02.025
更新日期：2010-04-30 00:00:00
abstract：:Chemotherapy results in a frequent yet poorly understood syndrome of long-term neurological deficits. Neural precursor cell dysfunction and white matter dysfunction are thought to contribute to this debilitating syndrome. Here, we demonstrate persistent depletion of oligodendrocyte lineage cells in humans who received...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.10.049
更新日期：2019-01-10 00:00:00
abstract：:Brown and beige adipocytes are specialized cells that express uncoupling protein 1 (UCP1) and dissipate chemical energy as heat. These cells likely possess alternative UCP1-independent thermogenic mechanisms. Here, we identify a secreted enzyme, peptidase M20 domain containing 1 (PM20D1), that is enriched in UCP1(+) v...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.05.071
更新日期：2016-07-14 00:00:00
abstract：:Loss of cell polarity proteins such as Scribble induces neoplasia in Drosophila by promoting uncontrolled proliferation. In mammals, the role that polarity proteins play during tumorigenesis is not well understood. Here, we demonstrate that depletion of Scribble in mammary epithelia disrupts cell polarity, blocks thre...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2008.09.045
更新日期：2008-11-28 00:00:00
abstract：:As more plant genome sequences become available, researchers are increasingly using comparative genomics to address some of the major questions in plant biology. Such questions include the evolution of photosynthesis and multicellularity, the developmental genetic changes responsible for alterations in body plan, and ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2007.04.004
更新日期：2007-04-20 00:00:00
abstract：:The intermediate filament proteins, desmin and vimentin, are phosphorylated in skeletal muscle cells in vivo. Desmin kinase activities have been purified from mature chicken skeletal muscle and identified as the cAMP-dependent kinases. Chicken skeletal muscle contains two cAMP-dependent protein kinases which are simil...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90278-6
更新日期：1981-01-01 00:00:00
abstract：:Oncogenic activation of RAS genes via point mutations occurs in 20%-30% of human cancers. The development of effective RAS inhibitors has been challenging, necessitating new approaches to inhibit this oncogenic protein. Functional studies have shown that the switch region of RAS interacts with a large number of effect...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.03.045
更新日期：2016-04-21 00:00:00
abstract：:The small ribonucleoproteins recognized by anti-La autoantibodies contain a heterogeneous mixture of small RNAs from uninfected mammalian cells. The identity of many of these has now been established by the discovery of precursor forms of 5S rRNA and of certain tRNAs among La RNAs from HeLa cells. The small fraction o...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(82)90099-x
更新日期：1982-05-01 00:00:00
abstract：:The exchange of metabolites between the mitochondrial matrix and the cytosol depends on β-barrel channels in the outer membrane and α-helical carrier proteins in the inner membrane. The essential translocase of the inner membrane (TIM) chaperones escort these proteins through the intermembrane space, but the structura...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.10.039
更新日期：2018-11-15 00:00:00
abstract：:We have used monoclonal antibodies to detect developmentally regulated cell surface antigens on D. discoideum amebae. A study of an antigen detected using an antibody produced by a hybridoma line implicates a previously undescribed component in the process of cell aggregation. This antigen (consisting of a doublet of ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90295-7
更新日期：1983-04-01 00:00:00
abstract：:The immunological synapse (IS) is a junction between the T cell and antigen-presenting cell and is composed of supramolecular activation clusters (SMACs). No studies have been published on naive T cell IS dynamics. Here, we find that IS formation during antigen recognition comprises cycles of stable IS formation and a...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2007.03.037
更新日期：2007-05-18 00:00:00
abstract：:Ocean microbial communities strongly influence the biogeochemistry, food webs, and climate of our planet. Despite recent advances in understanding their taxonomic and genomic compositions, little is known about how their transcriptomes vary globally. Here, we present a dataset of 187 metatranscriptomes and 370 metagen...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.10.014
更新日期：2019-11-14 00:00:00
abstract：:To determine the extent and nature of genetic variation present in independent isolates of HTLV-III/LAV, the nucleotide sequences of the entire envelope gene and parts of gag and pol were determined for two AIDS viruses. The results indicated that variation throughout the viral genome is extensive and that the envelop...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(86)90778-6
更新日期：1986-06-06 00:00:00
abstract：:The biguanide drug metformin is widely prescribed to treat type 2 diabetes and metabolic syndrome, but its mode of action remains uncertain. Metformin also increases lifespan in Caenorhabditis elegans cocultured with Escherichia coli. This bacterium exerts complex nutritional and pathogenic effects on its nematode pre...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.02.035
更新日期：2013-03-28 00:00:00
abstract：:In eukaryotes, a specialized pathway of mRNA degradation termed nonsense-mediated decay (NMD) functions in mRNA quality control by recognizing and degrading mRNAs with aberrant termination codons. We demonstrate that NMD in yeast targets premature termination codon (PTC)-containing mRNA to P-bodies. Upf1p is sufficien...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2006.04.037
更新日期：2006-06-16 00:00:00
abstract：:Clec16a has been identified as a disease susceptibility gene for type 1 diabetes, multiple sclerosis, and adrenal dysfunction, but its function is unknown. Here we report that Clec16a is a membrane-associated endosomal protein that interacts with E3 ubiquitin ligase Nrdp1. Loss of Clec16a leads to an increase in the N...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.05.016
更新日期：2014-06-19 00:00:00
abstract：:The 90S pre-ribosome is an early biogenesis intermediate formed during co-transcriptional ribosome formation, composed of ∼70 assembly factors and several small nucleolar RNAs (snoRNAs) that associate with nascent pre-rRNA. We report the cryo-EM structure of the Chaetomium thermophilum 90S pre-ribosome, revealing how ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.06.014
更新日期：2016-07-14 00:00:00
