Solving mysteries of DNA replication and frog cloning.


:Compared to sperm nuclei, nuclei from adult somatic cells replicate inefficiently in frog egg extract. In this issue of Cell, Lemaitre et al. (2005) show that pre-exposure of erythrocyte nuclei to a mitotic extract removes this difference, reorganizes the chromatin into shorter loops, and allows replication at much shorter intervals along the DNA. Remarkably, these observations also explain an old mystery of why serial nuclear transplantation was so successful for cloning frogs.






Laskey R




Has Abstract


2005-12-02 00:00:00
















  • Bcl-2 antiapoptotic proteins inhibit Beclin 1-dependent autophagy.

    abstract::Apoptosis and autophagy are both tightly regulated biological processes that play a central role in tissue homeostasis, development, and disease. The anti-apoptotic protein, Bcl-2, interacts with the evolutionarily conserved autophagy protein, Beclin 1. However, little is known about the functional significance of thi...


    pub_type: 杂志文章


    authors: Pattingre S,Tassa A,Qu X,Garuti R,Liang XH,Mizushima N,Packer M,Schneider MD,Levine B

    更新日期:2005-09-23 00:00:00

  • Targeted overactivity of beta cell K(ATP) channels induces profound neonatal diabetes.

    abstract::A paradigm for control of insulin secretion is that glucose metabolism elevates cytoplasmic [ATP]/[ADP] in beta cells, closing K(ATP) channels and causing depolarization, Ca2+ entry, and insulin release. Decreased responsiveness of K(ATP) channels to elevated [ATP]/[ADP] should therefore lead to decreased insulin secr...


    pub_type: 杂志文章


    authors: Koster JC,Marshall BA,Ensor N,Corbett JA,Nichols CG

    更新日期:2000-03-17 00:00:00

  • Switch between large hand-over-hand and small inchworm-like steps in myosin VI.

    abstract::Many biological motor molecules move within cells using stepsizes predictable from their structures. Myosin VI, however, has much larger and more broadly distributed stepsizes than those predicted from its short lever arms. We explain the discrepancy by monitoring Qdots and gold nanoparticles attached to the myosin-VI...


    pub_type: 杂志文章


    authors: Nishikawa S,Arimoto I,Ikezaki K,Sugawa M,Ueno H,Komori T,Iwane AH,Yanagida T

    更新日期:2010-09-17 00:00:00

  • Procollagen and collagen produced by a teratocarcinoma-derived cell line, TSD4: evidence for a new molecular form of collagen.

    abstract::Procollagen and collagen were isolated from the culture medium and cell layer of line TSD4 (obtained from mouse teratocarcinoma OTT6050). SDS-polyacrylamide gel electrophoresis of the highly purified procollagen fraction demonstrated that the fraction is composed of theta chains (150,000 daltons), pro alpha chains (13...


    pub_type: 杂志文章


    authors: Little CD,Church RL,Miller RA,Ruddle FH

    更新日期:1977-02-01 00:00:00

  • Promoter-selective activation domains in Oct-1 and Oct-2 direct differential activation of an snRNA and mRNA promoter.

    abstract::The promoter specificity of transcriptional activators is generally thought to be conferred by the specificity of the DNA-binding domain, which brings the activation domain to the appropriate promoter sequence. We show here, however, that Oct-1 and Oct-2 can differentially activate transcription not through DNA bindin...


    pub_type: 杂志文章


    authors: Tanaka M,Lai JS,Herr W

    更新日期:1992-02-21 00:00:00

  • Neuron-Astrocyte Metabolic Coupling Protects against Activity-Induced Fatty Acid Toxicity.

    abstract::Metabolic coordination between neurons and astrocytes is critical for the health of the brain. However, neuron-astrocyte coupling of lipid metabolism, particularly in response to neural activity, remains largely uncharacterized. Here, we demonstrate that toxic fatty acids (FAs) produced in hyperactive neurons are tran...


    pub_type: 杂志文章


    authors: Ioannou MS,Jackson J,Sheu SH,Chang CL,Weigel AV,Liu H,Pasolli HA,Xu CS,Pang S,Matthies D,Hess HF,Lippincott-Schwartz J,Liu Z

    更新日期:2019-05-30 00:00:00

  • Bringing Genetics Home.

    abstract::The promise of human genetics and genomics is nothing less than a fully tailored life, starting with medical treatments, diets, and preventative care all styled around a single genome. There's a lighter side as well where consumer genomics companies are helping people understand themselves and their families. Lara Sze...


    pub_type: 面试



    更新日期:2019-03-21 00:00:00

  • The tyrT locus: termination and processing of a complex transcript.

    abstract::The tyrT locus of E. coli contains a 208 bp spacer region that separates two copies of sequence encoding tRNATyr1. The spacer includes a 120 bp sequence that is homologous to a sequence that is repeated three times in the distal portion of the tyrT locus. The tyrT locus possesses a graded set of transcription terminat...


    pub_type: 杂志文章


    authors: Rossi J,Egan J,Hudson L,Landy A

    更新日期:1981-11-01 00:00:00

  • The path to perdition is paved with protons.

    abstract::Recent studies are beginning to shed light on the mechanism of ribosome-catalyzed peptide bond formation. ...


    pub_type: 杂志文章,评审


    authors: Green R,Lorsch JR

    更新日期:2002-09-20 00:00:00

  • Two modes of ligand recognition by TLRs.

    abstract::Toll-like receptors (TLRs) are membrane-bound sensors that detect and respond to microbial infection. Two studies in Cell, one in this issue, reveal how TLRs recognize their ligands. Kim et al. (2007) recently reported the structure of TLR4 bound to the accessory protein MD-2 and its antagonist, the drug eritoran. In ...


    pub_type: 评论,杂志文章


    authors: Brodsky I,Medzhitov R

    更新日期:2007-09-21 00:00:00

  • Migratory Neural Crest Cells Phagocytose Dead Cells in the Developing Nervous System.

    abstract::During neural tube closure and spinal cord development, many cells die in both the central and peripheral nervous systems (CNS and PNS, respectively). However, myeloid-derived professional phagocytes have not yet colonized the trunk region during early neurogenesis. How apoptotic cells are removed from this region dur...


    pub_type: 杂志文章


    authors: Zhu Y,Crowley SC,Latimer AJ,Lewis GM,Nash R,Kucenas S

    更新日期:2019-09-19 00:00:00

  • NMR structure of the bacteriophage lambda N peptide/boxB RNA complex: recognition of a GNRA fold by an arginine-rich motif.

    abstract::The structure of the complex formed by the arginine-rich motif of the transcriptional antitermination protein N of phage lambda and boxB RNA was determined by heteronuclear magnetic resonance spectroscopy. A bent alpha helix in N recognizes primarily the shape and negatively charged surface of the boxB hairpin through...


    pub_type: 杂志文章


    authors: Legault P,Li J,Mogridge J,Kay LE,Greenblatt J

    更新日期:1998-04-17 00:00:00

  • The hunger genes: pathways to obesity.

    abstract::The global rise in the prevalence of obesity and associated co-morbidities such as type 2 diabetes, cardiovascular disease, and cancer represents a major public health concern. The biological response to increased consumption of palatable foods or a reduction in energy expenditure is highly variable between individual...


    pub_type: 杂志文章,评审


    authors: van der Klaauw AA,Farooqi IS

    更新日期:2015-03-26 00:00:00

  • A deeper look into translation initiation.

    abstract::Eukaryotic translation initiation requires coordinated assembly of a remarkable array of initiation factors onto the small ribosomal subunit to select an appropriate mRNA start codon. Studies from Erzberger et al. and Hussain et al. bring new insights into this mechanism by looking at early and late initiation interme...


    pub_type: 评论,杂志文章


    authors: Korostelev AA

    更新日期:2014-10-23 00:00:00

  • The cellular mechanisms of neuronal swelling underlying cytotoxic edema.

    abstract::Cytotoxic brain edema triggered by neuronal swelling is the chief cause of mortality following brain trauma and cerebral infarct. Using fluorescence lifetime imaging to analyze contributions of intracellular ionic changes in brain slices, we find that intense Na(+) entry triggers a secondary increase in intracellular ...


    pub_type: 杂志文章


    authors: Rungta RL,Choi HB,Tyson JR,Malik A,Dissing-Olesen L,Lin PJC,Cain SM,Cullis PR,Snutch TP,MacVicar BA

    更新日期:2015-04-23 00:00:00

  • Calorie restriction--the SIR2 connection.

    abstract::A nutritious diet low in calories improves the health and extends the life span of rodents. Recent studies identified a gene, SIR2, which encodes an NAD-dependent deacetylase and may mediate the effects of calorie restriction. In this review, we discuss SIR2 genes and calorie restriction in the lower organisms yeast a...


    pub_type: 杂志文章,评审


    authors: Guarente L,Picard F

    更新日期:2005-02-25 00:00:00

  • The protein network of HIV budding.

    abstract::HIV release requires TSG101, a cellular factor that sorts proteins into vesicles that bud into multivesicular bodies (MVB). To test whether other proteins involved in MVB biogenesis (the class E proteins) also participate in HIV release, we identified 22 candidate human class E proteins. These proteins were connected ...


    pub_type: 杂志文章


    authors: von Schwedler UK,Stuchell M,Müller B,Ward DM,Chung HY,Morita E,Wang HE,Davis T,He GP,Cimbora DM,Scott A,Kräusslich HG,Kaplan J,Morham SG,Sundquist WI

    更新日期:2003-09-19 00:00:00

  • The oncogenic cysteine-rich LIM domain protein rbtn2 is essential for erythroid development.

    abstract::The LIM domain protein rbtn2 is associated with T cell acute leukemias. We demonstrate that rbtn2 is a nuclear protein expressed in the erythroid lineage in vivo, and using homologous recombination, we show that it is essential for erythroid development in mice. The homozygous rbtn2 null mutation leads to failure of y...


    pub_type: 杂志文章


    authors: Warren AJ,Colledge WH,Carlton MB,Evans MJ,Smith AJ,Rabbitts TH

    更新日期:1994-07-15 00:00:00

  • Degradation of mRNA in eukaryotes.

    abstract::Based on the above mechanisms of mRNA degradation, an integrated model of mRNA turnover can be proposed (Figure 1). In this model, all polyadenylated mRNAs would be degraded by the deadenylation-dependent pathway at some rate. In addition to this default pathway, another layer of complexity would come from degradation...


    pub_type: 杂志文章,评审


    authors: Beelman CA,Parker R

    更新日期:1995-04-21 00:00:00

  • Pattern abnormalities induced by ectopic expression of the Drosophila gene hairy are associated with repression of ftz transcription.

    abstract::The pair-rule segmentation gene hairy (h) is expressed and required in alternate metameres in the early Drosophila embryo. We show that h expression outside these domains, driven by an hsp70 promoter, suppresses the expression of fushi tarazu (ftz). The kinetics of action favor h acting directly as a transcriptional r...


    pub_type: 杂志文章


    authors: Ish-Horowicz D,Pinchin SM

    更新日期:1987-11-06 00:00:00

  • A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation.

    abstract::Many proteins contain disordered regions of low-sequence complexity, which cause aging-associated diseases because they are prone to aggregate. Here, we study FUS, a prion-like protein containing intrinsically disordered domains associated with the neurodegenerative disease ALS. We show that, in cells, FUS forms liqui...


    pub_type: 杂志文章


    authors: Patel A,Lee HO,Jawerth L,Maharana S,Jahnel M,Hein MY,Stoynov S,Mahamid J,Saha S,Franzmann TM,Pozniakovski A,Poser I,Maghelli N,Royer LA,Weigert M,Myers EW,Grill S,Drechsel D,Hyman AA,Alberti S

    更新日期:2015-08-27 00:00:00

  • A functional interaction between the C-terminal domain of RNA polymerase II and the negative regulator SIN1.

    abstract::The C-terminal domain (CTD) of the largest subunit of yeast RNA polymerase II contains 26-27 tandem copies of a conserved heptapeptide of unknown function. Yeast strains whose CTD contains ten heptamers are viable but defective for transcription of the INO1 gene and cold sensitive for growth. Deletion of the SIN1 gene...


    pub_type: 杂志文章


    authors: Peterson CL,Kruger W,Herskowitz I

    更新日期:1991-03-22 00:00:00

  • Cellular Control of Viscosity Counters Changes in Temperature and Energy Availability.

    abstract::Cellular functioning requires the orchestration of thousands of molecular interactions in time and space. Yet most molecules in a cell move by diffusion, which is sensitive to external factors like temperature. How cells sustain complex, diffusion-based systems across wide temperature ranges is unknown. Here, we uncov...


    pub_type: 杂志文章


    authors: Persson LB,Ambati VS,Brandman O

    更新日期:2020-12-10 00:00:00

  • The ovalbumin gene: alleles created by mutations in the intervening sequences of the natural gene.

    abstract::Two allelic forms of the natural chicken ovalbumin gene have been independently cloned. These alleles differ from each other by an Eco RI restriction cleavage site in one of the seven intervening sequences within the natural ovalbumin gene. Restriction endonuclease mapping and sequence analyses of these cloned genotyp...


    pub_type: 杂志文章


    authors: Lai EC,Woo SL,Dugaiczyk A,O'Malley BW

    更新日期:1979-01-01 00:00:00

  • Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site.

    abstract::We show that expression of the iso-1-cytochrome c gene of Saccharomyces cerevisiae, CYC1, is tightly regulated by levels of intracellular heme. Expression is reduced at least 200-fold in cells grown under conditions of heme deficiency. Studies on the regulation of a CYC1-lacZ fused gene and direct determination of mRN...


    pub_type: 杂志文章


    authors: Guarente L,Mason T

    更新日期:1983-04-01 00:00:00

  • A secondary attachment site for bacteriophage lambda in trpC of E. coli.

    abstract::We have determined the nucleotide sequence of a secondary lambda attachment site in trpC. Direct sequence analysis of lambdatrp transducing phage DNA fragments carrying the two prophage attachment sites reveals a 6 nucleotide homology in the crossover region which is a subset of the 15 nucleotide core sequence in the ...


    pub_type: 杂志文章


    authors: Christie GE,Platt T

    更新日期:1979-02-01 00:00:00

  • Coupling of a replicative polymerase and helicase: a tau-DnaB interaction mediates rapid replication fork movement.

    abstract::The E. coli replication fork synthesizes DNA at the rate of nearly 1000 nt/s. We show here that an interaction between the tau subunit of the replicative polymerase (the DNA polymerase III holoenzyme) and the replication fork DNA helicase (DnaB) is required to mediate this high rate of replication fork movement. In th...


    pub_type: 杂志文章


    authors: Kim S,Dallmann HG,McHenry CS,Marians KJ

    更新日期:1996-02-23 00:00:00

  • Phosphotyrosine signaling: evolving a new cellular communication system.

    abstract::Tyrosine phosphorylation controls many cellular functions. Yet the three-part toolkit that regulates phosphotyrosine signaling-tyrosine kinases, phosphotyrosine phosphatases, and Src Homology 2 (SH2) domains-is a relatively new innovation. Genomic analyses reveal how this revolutionary signaling system may have origin...


    pub_type: 杂志文章


    authors: Lim WA,Pawson T

    更新日期:2010-09-03 00:00:00

  • Hexosamine pathway metabolites enhance protein quality control and prolong life.

    abstract::Aging entails a progressive decline in protein homeostasis, which often leads to age-related diseases. The endoplasmic reticulum (ER) is the site of protein synthesis and maturation for secreted and membrane proteins. Correct folding of ER proteins requires covalent attachment of N-linked glycan oligosaccharides. Here...


    pub_type: 杂志文章


    authors: Denzel MS,Storm NJ,Gutschmidt A,Baddi R,Hinze Y,Jarosch E,Sommer T,Hoppe T,Antebi A

    更新日期:2014-03-13 00:00:00

  • Genomic Imprinting and Physiological Processes in Mammals.

    abstract::Complex multicellular organisms, such as mammals, express two complete sets of chromosomes per nucleus, combining the genetic material of both parents. However, epigenetic studies have demonstrated violations to this rule that are necessary for mammalian physiology; the most notable parental allele expression phenomen...


    pub_type: 杂志文章,评审


    authors: Tucci V,Isles AR,Kelsey G,Ferguson-Smith AC,Erice Imprinting Group.

    更新日期:2019-02-21 00:00:00