Mechanobiology of Protein Droplets: Force Arises from Disorder.


:The use of optogenetic approaches has revealed new roles for intracellular protein condensates described in two papers in this issue of Cell (Bracha et. al., 2018; Shin et al., 2018). These results show that growing condensates are able to exert mechanical forces resulting in chromatin rearrangement, establishing a new role for liquid-liquid phase separation in the mechanobiology of the cell.






Welsh TJ,Shen Y,Levin A,Knowles TPJ




Has Abstract


2018-11-29 00:00:00
















  • The primary structure of the putative oncogene pim-1 shows extensive homology with protein kinases.

    abstract::We have shown previously that the putative oncogene pim-1 is frequently activated by provirus insertion in murine leukemia virus-induced T cell lymphomas. Here we describe the structure of the pim-1 gene as determined by sequencing genomic and cDNA clones. The gene has an open reading frame, encoding a protein of 313 ...


    pub_type: 杂志文章


    authors: Selten G,Cuypers HT,Boelens W,Robanus-Maandag E,Verbeek J,Domen J,van Beveren C,Berns A

    更新日期:1986-08-15 00:00:00

  • TGFbeta primes breast tumors for lung metastasis seeding through angiopoietin-like 4.

    abstract::Cells released from primary tumors seed metastases to specific organs by a nonrandom process, implying the involvement of biologically selective mechanisms. Based on clinical, functional, and molecular evidence, we show that the cytokine TGFbeta in the breast tumor microenvironment primes cancer cells for metastasis t...


    pub_type: 杂志文章


    authors: Padua D,Zhang XH,Wang Q,Nadal C,Gerald WL,Gomis RR,Massagué J

    更新日期:2008-04-04 00:00:00

  • Translocation-capture sequencing reveals the extent and nature of chromosomal rearrangements in B lymphocytes.

    abstract::Chromosomal rearrangements, including translocations, require formation and joining of DNA double strand breaks (DSBs). These events disrupt the integrity of the genome and are frequently involved in producing leukemias, lymphomas and sarcomas. Despite the importance of these events, current understanding of their gen...


    pub_type: 杂志文章


    authors: Klein IA,Resch W,Jankovic M,Oliveira T,Yamane A,Nakahashi H,Di Virgilio M,Bothmer A,Nussenzweig A,Robbiani DF,Casellas R,Nussenzweig MC

    更新日期:2011-09-30 00:00:00

  • Point mutation at the ATP binding site of EGF receptor abolishes protein-tyrosine kinase activity and alters cellular routing.

    abstract::Cultured NIH 3T3 cells devoid of endogenous EGF receptors were transfected with cDNA constructs encoding either the human EGF receptor or an EGF receptor mutant in which Lys721, a key residue in the ATP binding site, was replaced with an alanine residue. The mutant receptor was properly processed, and it displayed bot...


    pub_type: 杂志文章


    authors: Honegger AM,Dull TJ,Felder S,Van Obberghen E,Bellot F,Szapary D,Schmidt A,Ullrich A,Schlessinger J

    更新日期:1987-10-23 00:00:00

  • Proliferation, senescence, and neoplastic progression of beta cells in hyperplasic pancreatic islets.

    abstract::Three different cases of pancreatic beta cell hyperplasia in mice are accompanied by an increase in a subclass of cells expressing tyrosine hydroxylase (TH), a neuronal enzyme. In the nontumorigenic cases of islet growth during normal pregnancy and in the obese mutant mouse, the TH-insulin cells do not divide, in cont...


    pub_type: 杂志文章


    authors: Teitelman G,Alpert S,Hanahan D

    更新日期:1988-01-15 00:00:00

  • Complement C1q activates canonical Wnt signaling and promotes aging-related phenotypes.

    abstract::Wnt signaling plays critical roles in development of various organs and pathogenesis of many diseases, and augmented Wnt signaling has recently been implicated in mammalian aging and aging-related phenotypes. We here report that complement C1q activates canonical Wnt signaling and promotes aging-associated decline in ...


    pub_type: 杂志文章


    authors: Naito AT,Sumida T,Nomura S,Liu ML,Higo T,Nakagawa A,Okada K,Sakai T,Hashimoto A,Hara Y,Shimizu I,Zhu W,Toko H,Katada A,Akazawa H,Oka T,Lee JK,Minamino T,Nagai T,Walsh K,Kikuchi A,Matsumoto M,Botto M,Shiojima

    更新日期:2012-06-08 00:00:00

  • Mechanisms of conformational change for a replicative hexameric helicase of SV40 large tumor antigen.

    abstract::The large tumor antigen (LTag) of simian virus 40, an AAA(+) protein, is a hexameric helicase essential for viral DNA replication in eukaryotic cells. LTag functions as an efficient molecular machine powered by ATP binding and hydrolysis for origin DNA melting and replication fork unwinding. To understand how ATP bind...


    pub_type: 杂志文章


    authors: Gai D,Zhao R,Li D,Finkielstein CV,Chen XS

    更新日期:2004-10-01 00:00:00

  • Integrated viral sequences in adenovirus type 12-transformed hamster cells.

    abstract::The physical state of the viral genome in four lines of hamster cells transformed by adenovirus type 12 (Ad12) has been investigated. The four lines of transformed cells originated from hamster cells after infection with Ad12 at multiplicities ranging from 5-350 plaque-forming units per cell. The DNA from transformed ...


    pub_type: 杂志文章


    authors: Groneberg J,Chardonnet Y,Doerfler W

    更新日期:1977-01-01 00:00:00

  • RNase III cleaves eukaryotic preribosomal RNA at a U3 snoRNP-dependent site.

    abstract::A yeast gene homologous to bacterial RNase III (RNT1) encodes a double-strand-specific endoribonuclease essential for ribosome synthesis. Two rRNA processing events are blocked in cells temperature sensitive for RNT1: cleavage at the snoRNA-dependent AO site in the 5' ETS and cleavage in the 3' ETS. Recombinant RNT1 p...


    pub_type: 杂志文章


    authors: Elela SA,Igel H,Ares M Jr

    更新日期:1996-04-05 00:00:00

  • Blinded by the Light: The Growing Complexity of p53.

    abstract::While the tumor suppressor functions of p53 have long been recognized, the contribution of p53 to numerous other aspects of disease and normal life is only now being appreciated. This burgeoning range of responses to p53 is reflected by an increasing variety of mechanisms through which p53 can function, although the a...


    pub_type: 杂志文章,评审


    authors: Vousden KH,Prives C

    更新日期:2009-05-01 00:00:00

  • Replication Timing Becomes Intertwined with 3D Genome Organization.

    abstract::Through dissecting the link between spatial genome organization and DNA replication timing, Sima et al. (2018) discover early replicating control elements (ERCEs), a new type of cis-acting elements that regulate replication timing, transcription, and multiple layers of three-dimensional features of genome organization...


    pub_type: 评论,杂志文章


    authors: Ma J,Duan Z

    更新日期:2019-02-07 00:00:00

  • New "hogs" in Hedgehog transport and signal reception.

    abstract::A recent paper in Cell (Yao et al., 2006) and two papers in Developmental Cell (Tenzen et al., 2006; Zhang et al., 2006) identify a new receptor component for Hedgehog, a key morphogen in embryonic development. Many other proteins that bind to Hedgehog in the extracellular matrix or on the cell surface have been ident...


    pub_type: 评论,杂志文章,评审


    authors: Wilson CW,Chuang PT

    更新日期:2006-05-05 00:00:00

  • A eukaryotic-like Ser/Thr kinase signals bacteria to exit dormancy in response to peptidoglycan fragments.

    abstract::Bacteria can respond to adverse environmental conditions by drastically reducing or even ceasing metabolic activity. They must then determine that conditions have improved before exiting dormancy, and one indication of such a change is the growth of other bacteria in the local environment. Growing bacteria release mur...


    pub_type: 杂志文章


    authors: Shah IM,Laaberki MH,Popham DL,Dworkin J

    更新日期:2008-10-31 00:00:00

  • Mapping human epigenomes.

    abstract::As the second dimension to the genome, the epigenome contains key information specific to every type of cells. Thousands of human epigenome maps have been produced in recent years thanks to rapid development of high throughput epigenome mapping technologies. In this review, we discuss the current epigenome mapping too...


    pub_type: 杂志文章,评审


    authors: Rivera CM,Ren B

    更新日期:2013-09-26 00:00:00

  • SARA-regulated vesicular targeting underlies formation of the light-sensing organelle in mammalian rods.

    abstract::The light-sensing organelle of the vertebrate rod photoreceptor, the outer segment (OS), is a modified cilium containing approximately 1,000 stacked disc membranes that are densely packed with visual pigment rhodopsin. The mammalian OS is renewed every ten days; new discs are assembled at the base of the OS by a poorl...


    pub_type: 杂志文章


    authors: Chuang JZ,Zhao Y,Sung CH

    更新日期:2007-08-10 00:00:00

  • Genetic evidence that Tn10 transposes by a nonreplicative mechanism.

    abstract::We present genetic evidence that the tetracycline resistance element Tn10 transposes by a nonreplicative mechanism. Heteroduplex Tn10 elements containing three single base pair mismatches were constructed on lambda phage genomes and allowed to transpose from lambda into the bacterial chromosome. Analysis of TetR colon...


    pub_type: 杂志文章


    authors: Bender J,Kleckner N

    更新日期:1986-06-20 00:00:00

  • Identification of selective inhibitors of cancer stem cells by high-throughput screening.

    abstract::Screens for agents that specifically kill epithelial cancer stem cells (CSCs) have not been possible due to the rarity of these cells within tumor cell populations and their relative instability in culture. We describe here an approach to screening for agents with epithelial CSC-specific toxicity. We implemented this ...


    pub_type: 杂志文章


    authors: Gupta PB,Onder TT,Jiang G,Tao K,Kuperwasser C,Weinberg RA,Lander ES

    更新日期:2009-08-21 00:00:00

  • GM130 Regulates Golgi-Derived Spindle Assembly by Activating TPX2 and Capturing Microtubules.

    abstract::Spindle assembly requires the coordinated action of multiple cellular structures to nucleate and organize microtubules in a precise spatiotemporal manner. Among them, the contributions of centrosomes, chromosomes, and microtubules have been well studied, yet the involvement of membrane-bound organelles remains largely...


    pub_type: 杂志文章


    authors: Wei JH,Zhang ZC,Wynn RM,Seemann J

    更新日期:2015-07-16 00:00:00

  • Travel Less. Make It Worthwhile.

    abstract::Academic travel has a substantial carbon footprint. The ongoing pandemic has propelled the development and adoption of technologies for online delivery of seminars and remote attendance at scientific conferences. This should not lead to the complete elimination of in-person events, but the scientific community must se...


    pub_type: 杂志文章


    authors: Geitmann A

    更新日期:2020-08-20 00:00:00

  • RAP1 is essential for silencing telomeric variant surface glycoprotein genes in Trypanosoma brucei.

    abstract::Trypanosoma brucei expresses variant surface glycoprotein (VSG) genes in a strictly monoallelic fashion in its mammalian hosts, but it is unclear how this important virulence mechanism is enforced. Telomere position effect, an epigenetic phenomenon, has been proposed to play a critical role in VSG regulation, yet no t...


    pub_type: 杂志文章


    authors: Yang X,Figueiredo LM,Espinal A,Okubo E,Li B

    更新日期:2009-04-03 00:00:00

  • Critical sequences within mitochondrial introns: pleiotropic mRNA maturase and cis-dominant signals of the box intron controlling reductase and oxidase.

    abstract::We have established the DNA sequence of nine yeast mutants that prevent the expression either of the split cytochrome b gene alone (five mutants) or of two split genes, the cytochrome b gene and the cytochrome oxidase subunit I gene (four mutants). All the mutations analyzed are localized in intron 14 of the cob-box g...


    pub_type: 杂志文章


    authors: De La Salle H,Jacq C,Slonimski PP

    更新日期:1982-04-01 00:00:00

  • AgRP Neurons Control Systemic Insulin Sensitivity via Myostatin Expression in Brown Adipose Tissue.

    abstract::Activation of Agouti-related peptide (AgRP) neurons potently promotes feeding, and chronically altering their activity also affects peripheral glucose homeostasis. We demonstrate that acute activation of AgRP neurons causes insulin resistance through impairment of insulin-stimulated glucose uptake into brown adipose t...


    pub_type: 杂志文章


    authors: Steculorum SM,Ruud J,Karakasilioti I,Backes H,Engström Ruud L,Timper K,Hess ME,Tsaousidou E,Mauer J,Vogt MC,Paeger L,Bremser S,Klein AC,Morgan DA,Frommolt P,Brinkkötter PT,Hammerschmidt P,Benzing T,Rahmouni K,Wunder

    更新日期:2016-03-24 00:00:00

  • Differentiation in the salivary glands of Drosophila melanogaster: characterization of the glue proteins and their developmental appearance.

    abstract::The larval salivary glands of Drosophila melanogaster, which are widely used for cytogenic studies, are also useful for studying the regulation of specific protein synthesis during differentiation. These glands synthesize and secrete massive amounts of a glue which attaches the pupae to the substrate during metamorpho...


    pub_type: 杂志文章


    authors: Beckendorf SK,Kafatos FC

    更新日期:1976-11-01 00:00:00

  • The spliceosome: design principles of a dynamic RNP machine.

    abstract::Ribonucleoproteins (RNPs) mediate key cellular functions such as gene expression and its regulation. Whereas most RNP enzymes are stable in composition and harbor preformed active sites, the spliceosome, which removes noncoding introns from precursor messenger RNAs (pre-mRNAs), follows fundamentally different strategi...


    pub_type: 杂志文章,评审


    authors: Wahl MC,Will CL,Lührmann R

    更新日期:2009-02-20 00:00:00

  • Promoter-selective activation domains in Oct-1 and Oct-2 direct differential activation of an snRNA and mRNA promoter.

    abstract::The promoter specificity of transcriptional activators is generally thought to be conferred by the specificity of the DNA-binding domain, which brings the activation domain to the appropriate promoter sequence. We show here, however, that Oct-1 and Oct-2 can differentially activate transcription not through DNA bindin...


    pub_type: 杂志文章


    authors: Tanaka M,Lai JS,Herr W

    更新日期:1992-02-21 00:00:00

  • Sugar Synthesis from CO2 in Escherichia coli.

    abstract::Can a heterotrophic organism be evolved to synthesize biomass from CO2 directly? So far, non-native carbon fixation in which biomass precursors are synthesized solely from CO2 has remained an elusive grand challenge. Here, we demonstrate how a combination of rational metabolic rewiring, recombinant expression, and lab...


    pub_type: 杂志文章


    authors: Antonovsky N,Gleizer S,Noor E,Zohar Y,Herz E,Barenholz U,Zelcbuch L,Amram S,Wides A,Tepper N,Davidi D,Bar-On Y,Bareia T,Wernick DG,Shani I,Malitsky S,Jona G,Bar-Even A,Milo R

    更新日期:2016-06-30 00:00:00

  • Behind the movement.

    abstract::This year, the Albert Lasker Basic Medical Research Award will be shared by Michael Sheetz, James Spudich, and Ronald Vale for discoveries concerning the biophysical actions of cytoskeletal motor-protein machines that move cargo within cells, contract muscles, and enable cell motility. ...


    pub_type: 杂志文章


    authors: Ringe D,Petsko GA

    更新日期:2012-09-14 00:00:00

  • p53 regulation orchestrates the TGF-beta response.

    abstract::Among its multiple functions, p53 is a critical regulator of TGF-beta responses. Sasai et al. (2008) now identify a new p53 inhibitory protein, XFDL156. During embryonic development, this factor is expressed in the ectoderm germ layer and maintains the pluripotency of ectodermal cells by inhibiting TGF-beta target gen...


    pub_type: 评论,杂志文章


    authors: Piccolo S

    更新日期:2008-05-30 00:00:00

  • Mammalian prion biology: one century of evolving concepts.

    abstract::Prions have been responsible for an entire century of tragic episodes. Fifty years ago, kuru decimated the population of Papua New Guinea. Then, iatrogenic transmission of prions caused more than 250 cases of Creutzfeldt-Jakob disease. More recently, transmission of bovine spongiform encephalopathy to humans caused a ...


    pub_type: 杂志文章,评审


    authors: Aguzzi A,Polymenidou M

    更新日期:2004-01-23 00:00:00

  • Sequence of interrupted and uninterrupted mRNAs and cloned DNA coding for the two overlapping nonstructural proteins of influenza virus.

    abstract::We have obtained the complete sequence of cloned full-length DNA (NS DNA) derived from influenza virus gene 8, which codes for two unique polypeptides, NS1 and NS2, and the sequence of the NS2 mRNA. Previously we showed that the mRNA for NS1 (approximately 860 nucleotides) is colinear with the viral RNA and maps from ...


    pub_type: 杂志文章


    authors: Lamb RA,Lai CJ

    更新日期:1980-09-01 00:00:00