Structural basis for the methylation state-specific recognition of histone H4-K20 by 53BP1 and Crb2 in DNA repair.


:Histone lysine methylation has been linked to the recruitment of mammalian DNA repair factor 53BP1 and putative fission yeast homolog Crb2 to DNA double-strand breaks (DSBs), but how histone recognition is achieved has not been established. Here we demonstrate that this link occurs through direct binding of 53BP1 and Crb2 to histone H4. Using X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy, we show that, despite low amino acid sequence conservation, both 53BP1 and Crb2 contain tandem tudor domains that interact with histone H4 specifically dimethylated at Lys20 (H4-K20me2). The structure of 53BP1/H4-K20me2 complex uncovers a unique five-residue 53BP1 binding cage, remarkably conserved in the structure of Crb2, that best accommodates a dimethyllysine but excludes a trimethyllysine, thus explaining the methylation state-specific recognition of H4-K20. This study reveals an evolutionarily conserved molecular mechanism of targeting DNA repair proteins to DSBs by direct recognition of H4-K20me2.






Botuyan MV,Lee J,Ward IM,Kim JE,Thompson JR,Chen J,Mer G




Has Abstract


2006-12-29 00:00:00
















  • The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis.

    abstract::In this study, we have located the sites of transcription initiation and termination on a cloned fragment of ribosomal DNA from X. laevis, and have sequenced the surrounding nucleotides. As reported previously (Reeder, Sollner-Webb and Wahn, 1977), about 25% of the 40S rRNA precursor molecules isolated from oocytes ha...


    pub_type: 杂志文章


    authors: Sollner-Webb B,Reeder RH

    更新日期:1979-10-01 00:00:00

  • Mammalian prion biology: one century of evolving concepts.

    abstract::Prions have been responsible for an entire century of tragic episodes. Fifty years ago, kuru decimated the population of Papua New Guinea. Then, iatrogenic transmission of prions caused more than 250 cases of Creutzfeldt-Jakob disease. More recently, transmission of bovine spongiform encephalopathy to humans caused a ...


    pub_type: 杂志文章,评审


    authors: Aguzzi A,Polymenidou M

    更新日期:2004-01-23 00:00:00

  • Nerve growth factor is an autocrine survival factor for memory B lymphocytes.

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    pub_type: 杂志文章


    authors: Torcia M,Bracci-Laudiero L,Lucibello M,Nencioni L,Labardi D,Rubartelli A,Cozzolino F,Aloe L,Garaci E

    更新日期:1996-05-03 00:00:00

  • Structure of the murine immune response I-A beta locus: sequence of the I-A beta gene and an adjacent beta-chain second domain exon.

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    pub_type: 杂志文章


    authors: Larhammar D,Hammerling U,Denaro M,Lund T,Flavell RA,Rask L,Peterson PA

    更新日期:1983-08-01 00:00:00

  • E. coli and M. luteus DNA topoisomerase I can catalyze catenation of decatenation of double-stranded DNA rings.

    abstract::Escherichia coli and Micrococcus luteus DNA topoisomerase I are found to promote catenation of double-stranded DNA rings. At low DNA concentration dimeric catenanes are the major catenated products; at high DNA concentration or when spermidine is present, catenanes containing more than two rings are formed. There is n...


    pub_type: 杂志文章


    authors: Tse Y,Wang JC

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  • Redox regulation of a protein tyrosine kinase in the endoplasmic reticulum.

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    pub_type: 杂志文章


    authors: Bauskin AR,Alkalay I,Ben-Neriah Y

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  • Crystallographic analysis of anti-p24 (HIV-1) monoclonal antibody cross-reactivity and polyspecificity.

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    pub_type: 杂志文章


    authors: Keitel T,Kramer A,Wessner H,Scholz C,Schneider-Mergener J,Höhne W

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    pub_type: 杂志文章


    authors: Vaseva AV,Marchenko ND,Ji K,Tsirka SE,Holzmann S,Moll UM

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  • The BELL1 gene encodes a homeodomain protein involved in pattern formation in the Arabidopsis ovule primordium.

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    pub_type: 杂志文章


    authors: Reiser L,Modrusan Z,Margossian L,Samach A,Ohad N,Haughn GW,Fischer RL

    更新日期:1995-12-01 00:00:00

  • Screening Human Embryos for Polygenic Traits Has Limited Utility.

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    pub_type: 杂志文章


    authors: Karavani E,Zuk O,Zeevi D,Barzilai N,Stefanis NC,Hatzimanolis A,Smyrnis N,Avramopoulos D,Kruglyak L,Atzmon G,Lam M,Lencz T,Carmi S

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  • The key to left-right asymmetry.

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    pub_type: 杂志文章


    authors: Tabin CJ

    更新日期:2006-10-06 00:00:00

  • Circadian gene expression in individual fibroblasts: cell-autonomous and self-sustained oscillators pass time to daughter cells.

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    pub_type: 杂志文章


    authors: Nagoshi E,Saini C,Bauer C,Laroche T,Naef F,Schibler U

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  • Sequence organization of a cloned tDNA met fragment from Xenopus laevis.

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    pub_type: 杂志文章


    authors: Clarkson SG,Kurer V,Smith HO

    更新日期:1978-07-01 00:00:00

  • Chemical genetics: where genetics and pharmacology meet.

    abstract::Genetics and pharmacology can elicit surprisingly different phenotypes despite targeting the same protein. This Essay explores these unexpected differences and their implications for biology and medicine. ...


    pub_type: 杂志文章


    authors: Knight ZA,Shokat KM

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    pub_type: 评论,杂志文章


    authors: Deitsch KW

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    pub_type: 杂志文章


    authors: Ginsberg M,James D,Ding BS,Nolan D,Geng F,Butler JM,Schachterle W,Pulijaal VR,Mathew S,Chasen ST,Xiang J,Rosenwaks Z,Shido K,Elemento O,Rabbany SY,Rafii S

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    pub_type: 杂志文章,评审


    authors: Hynes RO

    更新日期:2003-06-27 00:00:00

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    pub_type: 杂志文章


    authors: Gomez-Ospina N,Tsuruta F,Barreto-Chang O,Hu L,Dolmetsch R

    更新日期:2006-11-03 00:00:00

  • Pathogenic simian immunodeficiency virus infection is associated with expansion of the enteric virome.

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    pub_type: 杂志文章


    authors: Handley SA,Thackray LB,Zhao G,Presti R,Miller AD,Droit L,Abbink P,Maxfield LF,Kambal A,Duan E,Stanley K,Kramer J,Macri SC,Permar SR,Schmitz JE,Mansfield K,Brenchley JM,Veazey RS,Stappenbeck TS,Wang D,Barouch DH,

    更新日期:2012-10-12 00:00:00

  • DNAase I sensitivity and methylation of active versus inactive rRNA genes in xenopus species hybrids.

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    pub_type: 杂志文章


    authors: Macleod D,Bird A

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  • Glucosylated hydroxymethyluracil, DNA base J, prevents transcriptional readthrough in Leishmania.

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    pub_type: 杂志文章


    authors: van Luenen HG,Farris C,Jan S,Genest PA,Tripathi P,Velds A,Kerkhoven RM,Nieuwland M,Haydock A,Ramasamy G,Vainio S,Heidebrecht T,Perrakis A,Pagie L,van Steensel B,Myler PJ,Borst P

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    pub_type: 杂志文章


    authors: McKeown AN,Bridgham JT,Anderson DW,Murphy MN,Ortlund EA,Thornton JW

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    pub_type: 杂志文章


    authors: Gardner JM,Hynes RO

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  • Bispecific Antibodies Against HIV.

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    pub_type: 评论,杂志文章


    authors: Montefiori DC

    更新日期:2016-06-16 00:00:00

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    pub_type: 杂志文章


    authors: Harris RS,Bishop KN,Sheehy AM,Craig HM,Petersen-Mahrt SK,Watt IN,Neuberger MS,Malim MH

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    pub_type: 杂志文章


    authors: Eagen KP,Hartl TA,Kornberg RD

    更新日期:2015-11-05 00:00:00

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    pub_type: 杂志文章


    authors: Jeppesen DK,Fenix AM,Franklin JL,Higginbotham JN,Zhang Q,Zimmerman LJ,Liebler DC,Ping J,Liu Q,Evans R,Fissell WH,Patton JG,Rome LH,Burnette DT,Coffey RJ

    更新日期:2019-04-04 00:00:00

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    pub_type: 杂志文章,评审


    authors: Rothman JE

    更新日期:1989-11-17 00:00:00

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    pub_type: 杂志文章


    authors: Bohr VA,Smith CA,Okumoto DS,Hanawalt PC

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    pub_type: 杂志文章


    authors: Liu HP,Bretscher A

    更新日期:1989-04-21 00:00:00