Structural basis for the methylation state-specific recognition of histone H4-K20 by 53BP1 and Crb2 in DNA repair.

abstract：:GAL4 derivatives containing an activation domain alleviated repression of a promoter during nucleosome assembly. A GAL4 derivative lacking an activation domain stably bound the promoter during nucleosome assembly but was not sufficient to preserve promoter function. The activation domain of GAL4 derivatives was essent...

journal_title：Cell

authors： Workman JL,Taylor IC,Kingston RE

更新日期：1991-02-08 00:00:00

abstract：:Enduring forms of synaptic plasticity and memory require new protein synthesis, but little is known about the underlying regulatory mechanisms. Here, we investigate the role of MAPK signaling in these processes. Conditional expression of a dominant-negative form of MEK1 in the postnatal murine forebrain inhibited ERK ...

journal_title：Cell

authors： Kelleher RJ 3rd,Govindarajan A,Jung HY,Kang H,Tonegawa S

更新日期：2004-02-06 00:00:00

abstract：:We have adopted Drosophila tissue culture cells as a host system for studying the structure and function of mammalian transcription factors. These cells provide an Sp1-deficient background and have been used in a complementation assay to identify functional domains of human transcription factor Sp1. The SV40 early pro...

journal_title：Cell

authors： Courey AJ,Tjian R

更新日期：1988-12-02 00:00:00

abstract：:The phosphoinositide 3-kinase related kinases (PIKKs) mediate responses to diverse stresses, including DNA double-strand breaks (DSBs), abnormal replication fork progression, the recognition of premature termination codons in mRNAs, and inadequate nutrient availability. Rigorous control of these kinases limits cellula...

journal_title：Cell

authors： Bakkenist CJ,Kastan MB

更新日期：2004-07-09 00:00:00

abstract：:D-type cyclins (cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. We tested the requirement for D-cyclins in mouse development and in proliferation by generating mice lacking all D-cyclins. We found that these cyclin D1(-/-)D2(-/-)D3(-/-) mice develop u...

journal_title：Cell

authors： Kozar K,Ciemerych MA,Rebel VI,Shigematsu H,Zagozdzon A,Sicinska E,Geng Y,Yu Q,Bhattacharya S,Bronson RT,Akashi K,Sicinski P

更新日期：2004-08-20 00:00:00

abstract：:The gut epithelium self-renews every several days, providing an important innate defense system that limits bacterial colonization. Nevertheless, many bacterial pathogens, including Shigella, efficiently colonize the intestinal epithelium. Here, we show that the Shigella effector IpaB, when delivered into epithelial c...

journal_title：Cell

authors： Iwai H,Kim M,Yoshikawa Y,Ashida H,Ogawa M,Fujita Y,Muller D,Kirikae T,Jackson PK,Kotani S,Sasakawa C

更新日期：2007-08-24 00:00:00

abstract：:Treatment of the kidney epithelial cell line MDCK with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) induces a large increase in the permeability of tight junctions within 2-4 hr. This change in transepithelial permeability is accompanied by alterations in cell morphology that include the opening of ti...

journal_title：Cell

authors： Ojakian GK

更新日期：1981-01-01 00:00:00

abstract：:Gastrointestinal enterochromaffin cells regulate bone and gut homeostasis via serotonin (5-hydroxytryptamine [5-HT]) production. A recent report suggested that gut microbes regulate 5-HT levels; however, the precise underlying molecular mechanisms are unexplored. Here, we reveal that the cation channel Piezo1 in the g...

journal_title：Cell

authors： Sugisawa E,Takayama Y,Takemura N,Kondo T,Hatakeyama S,Kumagai Y,Sunagawa M,Tominaga M,Maruyama K

更新日期：2020-08-06 00:00:00

abstract：:Rubisco, the key enzyme of CO2 fixation in photosynthesis, is prone to inactivation by inhibitory sugar phosphates. Inhibited Rubisco undergoes conformational repair by the hexameric AAA+ chaperone Rubisco activase (Rca) in a process that is not well understood. Here, we performed a structural and mechanistic analysis...

journal_title：Cell

authors： Flecken M,Wang H,Popilka L,Hartl FU,Bracher A,Hayer-Hartl M

更新日期：2020-10-15 00:00:00

abstract：:Vg1 is a TGF beta-related growth factor encoded by a maternal mRNA localized to vegetal blastomeres in Xenopus embryos. Vg1 precursor protein is abundant in vegetal cells, but the processed mature form has not been readily detected and no activity has been demonstrated for the putative Vg1 mature protein. We have engi...

journal_title：Cell

authors： Thomsen GH,Melton DA

更新日期：1993-08-13 00:00:00

abstract：:Secretory proteins are segregated from cytosolic proteins by their translocation into the endoplasmic reticulum (ER). A modified secretory protein trapped during translocation across the ER membrane can be crosslinked to two previously identified proteins, Sec61p and BiP (Kar2p). The dependence of this cross-linking u...

journal_title：Cell

authors： Sanders SL,Whitfield KM,Vogel JP,Rose MD,Schekman RW

更新日期：1992-04-17 00:00:00

abstract：:Mice homozygous for a targeted mutation of the Hoxa-2 (Hox 1.11) gene are born with cleft palates and die within 24 hr of birth. Analysis of stained skeletons revealed that homozygous mutant animals contained multiple cranial skeletal defects, including a duplication of the ossification centers of the bones of the mid...

journal_title：Cell

authors： Gendron-Maguire M,Mallo M,Zhang M,Gridley T

更新日期：1993-12-31 00:00:00

abstract：:We performed a comprehensive assessment of rare inherited variation in autism spectrum disorder (ASD) by analyzing whole-genome sequences of 2,308 individuals from families with multiple affected children. We implicate 69 genes in ASD risk, including 24 passing genome-wide Bonferroni correction and 16 new ASD risk gen...

journal_title：Cell

authors： Ruzzo EK,Pérez-Cano L,Jung JY,Wang LK,Kashef-Haghighi D,Hartl C,Singh C,Xu J,Hoekstra JN,Leventhal O,Leppä VM,Gandal MJ,Paskov K,Stockham N,Polioudakis D,Lowe JK,Prober DA,Geschwind DH,Wall DP

更新日期：2019-08-08 00:00:00

abstract：:The cytosolic protein APAF1, human homolog of C. elegans CED-4, participates in the CASPASE 9 (CASP9)-dependent activation of CASP3 in the general apoptotic pathway. We have generated by gene trap a null allele of the murine Apaf1. Homozygous mutants die at embryonic day 16.5. Their phenotype includes severe craniofac...

journal_title：Cell

authors： Cecconi F,Alvarez-Bolado G,Meyer BI,Roth KA,Gruss P

更新日期：1998-09-18 00:00:00

abstract：:How cells generate a single axis of polarity for mating, division, and movement is unknown. In this issue, Howell et al. (2009) use a synthetic biology approach to demonstrate that rapid competition for a soluble signaling component (Bem1) is essential to ensure a unique axis of polarity in budding yeast. ...

journal_title：Cell

authors： Houk AR,Millius A,Weiner OD

更新日期：2009-11-13 00:00:00

abstract：:We have determined the nucleotide sequence of the transposable maize controlling element Activator (Ac). The Ac element is 4563 bp long and has an imperfect terminal repetition of 11 bp. The element contains two open reading frames (ORF) encoding polypeptides of 839 and 210 amino acids. Evidence derived from structura...

journal_title：Cell

authors： Pohlman RF,Fedoroff NV,Messing J

更新日期：1984-06-01 00:00:00

abstract：:Mature HIV-1 particles contain conical-shaped capsids that enclose the viral RNA genome and perform essential functions in the virus life cycle. Previous structural analysis of two- and three-dimensional arrays of the capsid protein (CA) hexamer revealed three interfaces. Here, we present a cryoEM study of a tubular a...

journal_title：Cell

authors： Byeon IJ,Meng X,Jung J,Zhao G,Yang R,Ahn J,Shi J,Concel J,Aiken C,Zhang P,Gronenborn AM

更新日期：2009-11-13 00:00:00

abstract：:Many proteins (intrinsically disordered proteins, IDPs) or regions of proteins (intrinsically disordered regions, IDRs) lack a well-defined 3D structure under physiological conditions. Albeit unfolded and highly dynamic, these proteins are not denatured; rather, intrinsic structural disorder is their native, functiona...

journal_title：Cell

authors： Guharoy M,Pauwels K,Tompa P

更新日期：2015-05-21 00:00:00

abstract：:Kit/SCF signaling and Mitf-dependent transcription are both essential for melanocyte development and pigmentation. To identify Mitf-dependent Kit transcriptional targets in primary melanocytes, microarray studies were undertaken. Among identified targets was BCL2, whose germline deletion produces melanocyte loss and w...

journal_title：Cell

authors： McGill GG,Horstmann M,Widlund HR,Du J,Motyckova G,Nishimura EK,Lin YL,Ramaswamy S,Avery W,Ding HF,Jordan SA,Jackson IJ,Korsmeyer SJ,Golub TR,Fisher DE

更新日期：2002-06-14 00:00:00

abstract：:Expression of human immunodeficiency virus type 1 structural proteins requires both the viral Rev trans-activator and its cis-acting RNA target sequence, the Rev response element (RRE). The RRE has been mapped to a conserved region of the HIV-1 env gene and is predicted to form a complex, highly stable RNA stem-loop s...

journal_title：Cell

authors： Malim MH,Tiley LS,McCarn DF,Rusche JR,Hauber J,Cullen BR

更新日期：1990-02-23 00:00:00

abstract：:During Tetrahymena conjugation, programmed DNA degradation occurs in two separate nuclei. Thousands of germline-specific deletion elements are removed from the genome of the developing somatic macronucleus, and the old parental macronucleus is degraded by an apoptotic mechanism. An abundant polypeptide, Pdd1p (formerl...

journal_title：Cell

authors： Madireddi MT,Coyne RS,Smothers JF,Mickey KM,Yao MC,Allis CD

更新日期：1996-10-04 00:00:00

abstract：:The switch from membrane-bound to secreted-form IgM that occurs during differentiation of B lymphocytes has long been known to involve regulated processing of the heavy chain pre-mRNA. Here, we show that accumulation of one subunit of an essential polyadenylation factor (CstF-64) is specifically repressed in mouse pri...

journal_title：Cell

authors： Takagaki Y,Seipelt RL,Peterson ML,Manley JL

更新日期：1996-11-29 00:00:00

abstract：:A temperature-sensitive mutant of BHK, designated ts BN-2, shows a rapid drop in 3H-thymidine incorporation along with accumulation of the cells in the G1 phase of the cycle when asynchronous cultures are shifted from 33.5 degrees C to the nonpermissive temperature of 39.5 degrees C. Synchronized cultures of ts BN-2 c...

journal_title：Cell

authors： Nishimoto T,Eilen E,Basilico C

更新日期：1978-10-01 00:00:00

abstract：:Toxic DNA-protein crosslinks (DPCs) arise by ionizing irradiation and UV light, are particularly caused by endogenously produced reactive compounds such as formaldehyde, and also occur during compromised topoisomerase action. Although nucleotide excision repair and homologous recombination contribute to cell survival ...

journal_title：Cell

authors： Stingele J,Schwarz MS,Bloemeke N,Wolf PG,Jentsch S

更新日期：2014-07-17 00:00:00

abstract：SUMMARY:Disruption of the cyclin-dependent kinase-inhibitory domain of p27 enhances growth of mice. Growth is attributed to an increase in cell number, due to increased cell proliferation, most obviously in tissues that ordinarily express p27 at the highest levels. Disruption of p27 function leads to nodular hyperplasi...

journal_title：Cell

authors： Kiyokawa H,Kineman RD,Manova-Todorova KO,Soares VC,Hoffman ES,Ono M,Khanam D,Hayday AC,Frohman LA,Koff A

更新日期：1996-05-31 00:00:00

abstract：:N(6)-methyladenosine (m(6)A) is the most abundant internal modification of messenger RNA. While the presence of m(6)A on transcripts can impact nuclear RNA fates, a reader of this mark that mediates processing of nuclear transcripts has not been identified. We find that the RNA-binding protein HNRNPA2B1 binds m(6)A-be...

journal_title：Cell

authors： Alarcón CR,Goodarzi H,Lee H,Liu X,Tavazoie S,Tavazoie SF

更新日期：2015-09-10 00:00:00

abstract：:Hematopoietic stem cells (HSCs) exist in the bone marrow and circulate in the blood. In this issue, Massberg et al. (2007) report that HSCs also travel through the lymphatic system. Furthermore, migration of HSCs--which express Toll-like receptors--allows the recognition of pathogenic molecules in peripheral tissues t...

journal_title：Cell

authors： Welner RS,Kincade PW

更新日期：2007-11-30 00:00:00

abstract：:The expression of MHC class II genes is tightly regulated. One form of congenital severe combined immunodeficiency (SCID) is characterized by a regulatory defect that precludes expression of HLA class II genes. B lymphocyte cell lines from such SCID patients provide a tool for identifying putative regulatory proteins ...

journal_title：Cell

authors： Reith W,Satola S,Sanchez CH,Amaldi I,Lisowska-Grospierre B,Griscelli C,Hadam MR,Mach B

更新日期：1988-06-17 00:00:00

abstract：:Infections disturb metabolic homeostasis in many contexts, but the underlying connections are not completely understood. To address this, we use paired genetic and computational screens in Drosophila to identify transcriptional regulators of immunity and pathology and their associated target genes and physiologies. We...

journal_title：Cell

authors： Clark RI,Tan SW,Péan CB,Roostalu U,Vivancos V,Bronda K,Pilátová M,Fu J,Walker DW,Berdeaux R,Geissmann F,Dionne MS

更新日期：2013-10-10 00:00:00

abstract：:CFTR, the protein associated with cystic fibrosis, is phosphorylated on serine residues in response to cAMP agonists. Serines 660, 737, 795, and 813 were identified as in vivo targets for phosphorylation by protein kinase A. The SPQ fluorescence assay revealed that mutagenesis of any one of these sites did not affect ...

journal_title：Cell

authors： Cheng SH,Rich DP,Marshall J,Gregory RJ,Welsh MJ,Smith AE

更新日期：1991-09-06 00:00:00