Abstract：
:Treatment of the kidney epithelial cell line MDCK with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) induces a large increase in the permeability of tight junctions within 2-4 hr. This change in transepithelial permeability is accompanied by alterations in cell morphology that include the opening of tight junctions, loss of apical surface microvilli and the formation of cytoplasmic processes containing numerous microtubules, microfilaments and 10 nm intermediate filaments. Simultaneously, TPA also stimulates a 10-20 fold increase in the synthesis and secretion of the serine protease plasminogen activator (PA); however, the inhibition of PA synthesis by cycloheximide does not prevent the morphological changes or the increased tight junctional permeability, suggesting that PA is not involved in these early TPA-induced events. Drugs that inhibit cytoskeletal function, such as cytochalasins B and D, colchicine, and vinblastine sulfate, are all relatively effective in preventing the TPA-induced morphological changes but not the increases in transepithelial permeability. Our results provide evidence that possible sites of TPA action include epithelial tight junctions as well as components of the cytoskeleton.
journal_name
Celljournal_title
Cellauthors
Ojakian GKdoi
10.1016/0092-8674(81)90274-9subject
Has Abstractpub_date
1981-01-01 00:00:00pages
95-103issue
1eissn
0092-8674issn
1097-4172pii
0092-8674(81)90274-9journal_volume
23pub_type
杂志文章相关文献
CELL文献大全abstract：:Clarification of the information processing system in bacterial sensing has been obtained by studying mutants that lack the capacity to modify receptors covalently. The remaining part of the system is able to receive signals from the receptor, to respond with partial adaptation, and to exhibit a chemotactic response. ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(85)90125-4
更新日期：1985-09-01 00:00:00
abstract：:Cis-regulatory elements (CREs) are commonly recognized by correlative chromatin features, yet the molecular composition of the vast majority of CREs in chromatin remains unknown. Here, we describe a CRISPR affinity purification in situ of regulatory elements (CAPTURE) approach to unbiasedly identify locus-specific chr...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.08.003
更新日期：2017-08-24 00:00:00
abstract：:A growing body of evidence indicates that plant transcription factors move between cells. A recent paper by Nakajima et al. (2001) shows that movement of the SHORTROOT protein provides a mechanism for signaling positional information between cell layers of the root. ...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/s0092-8674(01)00532-3
更新日期：2001-10-19 00:00:00
abstract：:We have isolated the bovine and human genes for Müllerian inhibiting substance (MIS), a testicular glycoprotein that causes regression of the Müllerian duct during development of the male embryo. The mRNA sequence of bovine MIS, determined from an analysis of cDNA and genomic clones, codes for a protein of 575 amino a...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(86)90783-x
更新日期：1986-06-06 00:00:00
abstract：:Macroautophagy is a lysosomal degradative pathway essential for neuron survival. Here, we show that macroautophagy requires the Alzheimer's disease (AD)-related protein presenilin-1 (PS1). In PS1 null blastocysts, neurons from mice hypomorphic for PS1 or conditionally depleted of PS1, substrate proteolysis and autopha...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2010.05.008
更新日期：2010-06-25 00:00:00
abstract：:During embryogenesis, cells from the ventral and dorsal parts of the somites give rise to sclerotome and dermomyotome, respectively. Dermomyotome contains skeletal muscle precursors that are determined by the MyoD family of myogenic factors. We have isolated a novel myogenic repressor, I-mf (Inhibitor of MyoD family),...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80148-8
更新日期：1996-09-06 00:00:00
abstract：:Long chain fatty acids (LCFAs) are an important energy substrate used by cardiac myocytes and other cells, but the mechanism whereby these molecules cross the plasma membrane is poorly understood. We used an expression cloning strategy and a cDNA library from 3T3-L1 adipocytes to identify a cDNA that, when expressed i...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90252-6
更新日期：1994-11-04 00:00:00
abstract：:Genes are packaged into nucleosomal arrays, each nucleosome typically having two copies of histones H2A, H2B, H3, and H4. Histones have distinct posttranslational modifications, variant isoforms, and dynamics. Whether each histone copy within a nucleosome has distinct properties, particularly in relation to the direct...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.10.054
更新日期：2014-12-04 00:00:00
abstract：:Different mutations in the sqt-1 gene of C. elegans can lengthen, shorten, or helically twist the entire animal. We have cloned the sqt-1 gene and have shown that it encodes a collagen. sqt-1 was localized to a 35 kb region of DNA by physical mapping of chromosomal deficiencies. A transposon (Tc1)-induced mutation of ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(88)90214-0
更新日期：1988-11-18 00:00:00
abstract：:The adaptive immune system is important for control of most viral infections. The three fundamental components of the adaptive immune system are B cells (the source of antibodies), CD4+ T cells, and CD8+ T cells. The armamentarium of B cells, CD4+ T cells, and CD8+ T cells has differing roles in different viral infect...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2021.01.007
更新日期：2021-01-12 00:00:00
abstract：:Atopic dermatitis (AD) is a chronic itch and inflammatory disorder of the skin that affects one in ten people. Patients suffering from severe AD eventually progress to develop asthma and allergic rhinitis, in a process known as the "atopic march." Signaling between epithelial cells and innate immune cells via the cyto...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.08.057
更新日期：2013-10-10 00:00:00
abstract：:Humans are uniquely susceptible to typhoid fever caused by infection with Salmonella enterica serovar Typhi. Mathur et al. now report that mice lacking Toll-like receptor 11, which is absent in humans, can be lethally infected with S. Typhi, a breakthrough that promises to speed the development of better vaccines. ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2012.10.016
更新日期：2012-10-26 00:00:00
abstract：:In C. elegans, removing the germ cells extends life span by triggering the nuclear localization and activation of the DAF-16/FOXO transcription factor in the intestine. In this study, we identify and analyze genes required for germline removal to extend life span. We find that the reproductive system communicates with...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2006.01.039
更新日期：2006-03-10 00:00:00
abstract：:The molecular nature of mutations arising in dysgenic hybrids between P and M Drosophila melanogaster strains has been investigated. Seven independent mutations at the white locus were examined, and these fell into two classes on the basis of their genetic and structural properties. The five mutations comprising the f...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(82)90462-7
更新日期：1982-07-01 00:00:00
abstract：:Individual olfactory sensory neurons express only a single odorant receptor from a large family of genes, and this singularity is an essential feature in models of olfactory perception. We have devised a genetic strategy to examine the stability of receptor choice. We observe that immature olfactory sensory neurons th...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2004.05.015
更新日期：2004-06-11 00:00:00
abstract：:H2S is an endogenous gasotransmitter that plays an important role in physiological conditions. In this issue, Das et al. provide evidence that SIRT1-dependent angiogenesis is augmented by H2S-findings reinforced by Longchamp et al., who demonstrate that H2S-dependent angiogenesis is triggered by amino acid deprivation...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2018.03.011
更新日期：2018-03-22 00:00:00
abstract：:Adenovirus E1B 19 kDa protein protects against cell death induced by viral infection and certain external stimuli. The Bcl-2 protein can functionally substitute for the E1B 19 kDa protein. To identify cellular targets for the 19 kDa protein, we used the two-hybrid screen in yeast. We have isolated cDNAs for three diff...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90202-x
更新日期：1994-10-21 00:00:00
abstract：:The Hippo-YAP pathway regulates organ size by modulating cell proliferation and apoptosis. Yu et al. now reveal that G-protein-coupled receptors act upstream of the transcriptional coactivators YAP/TAZ. This study reinforces the connection between the actin cytoskeleton and Hippo pathway activity and identifies a clas...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2012.07.020
更新日期：2012-08-17 00:00:00
abstract：:The X-ray crystal structures of an anti-p24 (HIV-1) monoclonal antibody Fab fragment alone and in complexes with the epitope peptide GATPQDLNTnL (n = norleucine), an epitope-homologous peptide GATPEDLNQKLAGN, as well as two unrelated peptides GLYEWGGARITNTD and efslkGpllqwrsG (D-peptide), are presented to a maximum re...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80469-9
更新日期：1997-12-12 00:00:00
abstract：:The cytosolic protein APAF1, human homolog of C. elegans CED-4, participates in the CASPASE 9 (CASP9)-dependent activation of CASP3 in the general apoptotic pathway. We have generated by gene trap a null allele of the murine Apaf1. Homozygous mutants die at embryonic day 16.5. Their phenotype includes severe craniofac...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81732-8
更新日期：1998-09-18 00:00:00
abstract：:During fruiting body development, the product of the csgA gene is necessary for cellular aggregation, for spore differentiation, and for gene expression that is initiated after 6 hr of starvation. From nascent wild-type fruiting bodies we have purified a polypeptide of 17 kd called C-factor, which, at approximately 1 ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90211-v
更新日期：1990-04-06 00:00:00
abstract：:The global financial crisis has hit biotech companies hard both in the US and Europe as venture capital dries up. Finding new sources of long-term financing for translating research into new therapeutics will be essential for maintaining innovation and new drug development by biotech companies. ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2009.07.026
更新日期：2009-08-07 00:00:00
abstract：:Neurons in the cerebral cortex connect through descending pathways to hindbrain and spinal cord to activate muscle and generate movement. Although components of this pathway have been previously generated and studied in vitro, the assembly of this multi-synaptic circuit has not yet been achieved with human cells. Here...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.11.017
更新日期：2020-12-23 00:00:00
abstract：:Of the several families of adhesion receptors involved in leukocyte-endothelial cell interactions, only the selectins have been shown to initiate leukocyte interaction under physiologic shear; indeed, beta 2 (CD18) intergrins responsible for neutrophil arrest are unable to engage without prior selectin-mediated rollin...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90491-3
更新日期：1995-02-10 00:00:00
abstract：:The global rise in the prevalence of obesity and associated co-morbidities such as type 2 diabetes, cardiovascular disease, and cancer represents a major public health concern. The biological response to increased consumption of palatable foods or a reduction in energy expenditure is highly variable between individual...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2015.03.008
更新日期：2015-03-26 00:00:00
abstract：:In nearly every organism studied, reduced caloric intake extends life span. In yeast, span extension from dietary restriction is thought to be mediated by the highly conserved, nutrient-responsive target of rapamycin (TOR), protein kinase A (PKA), and Sch9 kinases. These kinases coordinately regulate various cellular ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2008.02.037
更新日期：2008-04-18 00:00:00
abstract：:Fos and Jun transcription factors are induced by a variety of extracellular signaling agents. We describe here an unusual member of the Fos family that is also induced, namely, a truncated form of FosB (delta FosB) missing the C-terminal 101 amino acids of FosB. delta FosB retains the dimerization and DNA-binding acti...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90504-r
更新日期：1991-02-22 00:00:00
abstract：:In embryonal carcinoma (EC) cells retinoic acid (RA) strongly induces transcription from the RA receptor beta 2 (RAR beta 2) promoter through an RA response element (RARE) located in close proximity to the TATA box. Here we demonstrate that recombinant human TATA box-binding protein, hTFIID, and RAR functionally coope...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90443-g
更新日期：1992-05-01 00:00:00
abstract：:Analysis of the in vitro splicing products of RNA precursors containing tandem duplications of the 5' or 3' splice sites of human beta-globin IVS 1 revealed that exon sequences play an important role in the relative use of the duplicated sites. These studies also show that the proximity of the 5' and 3' splice sites i...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(86)90343-0
更新日期：1986-08-29 00:00:00
abstract：:We have cloned the C. elegans cell death gene ced-3. A ced-3 transcript is most abundant during embryogenesis, the stage during which most programmed cell deaths occur. The predicted CED-3 protein shows similarity to human and murine interleukin-1 beta-converting enzyme and to the product of the mouse nedd-2 gene, whi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(93)90485-9
更新日期：1993-11-19 00:00:00
