The molecular basis of P-M hybrid dysgenesis: the nature of induced mutations.


:The molecular nature of mutations arising in dysgenic hybrids between P and M Drosophila melanogaster strains has been investigated. Seven independent mutations at the white locus were examined, and these fell into two classes on the basis of their genetic and structural properties. The five mutations comprising the first class were caused by DNA insertions of 0.5, 0.5, 0.6, 1.2 and 1.4 kb, respectively. The DNA insertions in four of these mutations were examined in detail. Although heterogeneous in size and pattern of restriction enzyme sites, they were homologous in sequence. We refer to members of this sequence family as P elements. Mutations caused by P elements appeared to be stable in the P cytotype, but had reversion rates greater than 10(-3) in the M cytotype. Phenotypic reversion to wild-type was accompanied by excision of the P element. The two mutations comprising the second class were caused by insertion of the 5.0 kb copia element and appeared to be stable in both P and M cytotypes.






Rubin GM,Kidwell MG,Bingham PM




Has Abstract


1982-07-01 00:00:00
















  • Hematopoiesis: an evolving paradigm for stem cell biology.

    abstract::Establishment and maintenance of the blood system relies on self-renewing hematopoietic stem cells (HSCs) that normally reside in small numbers in the bone marrow niche of adult mammals. This Review describes the developmental origins of HSCs and the molecular mechanisms that regulate lineage-specific differentiation....


    pub_type: 杂志文章


    authors: Orkin SH,Zon LI

    更新日期:2008-02-22 00:00:00

  • Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression.

    abstract::Targeted gene regulation on a genome-wide scale is a powerful strategy for interrogating, perturbing, and engineering cellular systems. Here, we develop a method for controlling gene expression based on Cas9, an RNA-guided DNA endonuclease from a type II CRISPR system. We show that a catalytically dead Cas9 lacking en...


    pub_type: 杂志文章


    authors: Qi LS,Larson MH,Gilbert LA,Doudna JA,Weissman JS,Arkin AP,Lim WA

    更新日期:2013-02-28 00:00:00

  • Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development.

    abstract::The cytosolic protein APAF1, human homolog of C. elegans CED-4, participates in the CASPASE 9 (CASP9)-dependent activation of CASP3 in the general apoptotic pathway. We have generated by gene trap a null allele of the murine Apaf1. Homozygous mutants die at embryonic day 16.5. Their phenotype includes severe craniofac...


    pub_type: 杂志文章


    authors: Cecconi F,Alvarez-Bolado G,Meyer BI,Roth KA,Gruss P

    更新日期:1998-09-18 00:00:00

  • Interhomolog bias during meiotic recombination: meiotic functions promote a highly differentiated interhomolog-only pathway.

    abstract::Meiotic recombination occurs preferentially between homologous nonsister chromatids rather than between sisters, opposite to the bias of mitotic recombinational repair. We have examined formation of joint molecule recombination intermediates (JMs) between homologs and between sisters in yeast strains lacking the meiot...


    pub_type: 杂志文章


    authors: Schwacha A,Kleckner N

    更新日期:1997-09-19 00:00:00

  • A Forward Chemical Genetic Screen Reveals Gut Microbiota Metabolites That Modulate Host Physiology.

    abstract::The intestinal microbiota produces tens of thousands of metabolites. Here, we used host sensing of small molecules by G-protein coupled receptors (GPCRs) as a lens to illuminate bioactive microbial metabolites that impact host physiology. We screened 144 human gut bacteria against the non-olfactory GPCRome and identif...


    pub_type: 杂志文章


    authors: Chen H,Nwe PK,Yang Y,Rosen CE,Bielecka AA,Kuchroo M,Cline GW,Kruse AC,Ring AM,Crawford JM,Palm NW

    更新日期:2019-05-16 00:00:00

  • Ligand-activated thyroid hormone and retinoic acid receptors inhibit growth factor receptor promoter expression.

    abstract::Thyroid hormone (T3) and retinoic acid (RA) receptors mediate ligand-dependent inhibition of epidermal growth factor (EGF) receptor and c-erbB2/neu promoter activities. Ligand-activated T3 and RA receptors act via a 36 bp 5' fragment of the EGF receptor gene in vivo and, in the presence of nuclear extract, bind with h...


    pub_type: 杂志文章


    authors: Hudson LG,Santon JB,Glass CK,Gill GN

    更新日期:1990-09-21 00:00:00

  • Decoupling the Functional Pleiotropy of Stem Cell Factor by Tuning c-Kit Signaling.

    abstract::Most secreted growth factors and cytokines are functionally pleiotropic because their receptors are expressed on diverse cell types. While important for normal mammalian physiology, pleiotropy limits the efficacy of cytokines and growth factors as therapeutics. Stem cell factor (SCF) is a growth factor that acts throu...


    pub_type: 杂志文章


    authors: Ho CCM,Chhabra A,Starkl P,Schnorr PJ,Wilmes S,Moraga I,Kwon HS,Gaudenzio N,Sibilano R,Wehrman TS,Gakovic M,Sockolosky JT,Tiffany MR,Ring AM,Piehler J,Weissman IL,Galli SJ,Shizuru JA,Garcia KC

    更新日期:2017-03-09 00:00:00

  • The key to left-right asymmetry.

    abstract::Establishment of left-right asymmetry in vertebrates involves cilia as essential components in the breaking of symmetry, an asymmetric signaling cascade, and a midline barrier that helps to maintain asymmetry. A new study suggests that a reaction-diffusion mechanism also plays a key role. ...


    pub_type: 杂志文章


    authors: Tabin CJ

    更新日期:2006-10-06 00:00:00

  • A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks.

    abstract::Finding the components of cellular circuits and determining their functions systematically remains a major challenge in mammalian cells. Here, we introduced genome-wide pooled CRISPR-Cas9 libraries into dendritic cells (DCs) to identify genes that control the induction of tumor necrosis factor (Tnf) by bacterial lipop...


    pub_type: 杂志文章


    authors: Parnas O,Jovanovic M,Eisenhaure TM,Herbst RH,Dixit A,Ye CJ,Przybylski D,Platt RJ,Tirosh I,Sanjana NE,Shalem O,Satija R,Raychowdhury R,Mertins P,Carr SA,Zhang F,Hacohen N,Regev A

    更新日期:2015-07-30 00:00:00

  • The NAD(+)/Sirtuin Pathway Modulates Longevity through Activation of Mitochondrial UPR and FOXO Signaling.

    abstract::NAD(+) is an important cofactor regulating metabolic homeostasis and a rate-limiting substrate for sirtuin deacylases. We show that NAD(+) levels are reduced in aged mice and Caenorhabditis elegans and that decreasing NAD(+) levels results in a further reduction in worm lifespan. Conversely, genetic or pharmacological...


    pub_type: 杂志文章


    authors: Mouchiroud L,Houtkooper RH,Moullan N,Katsyuba E,Ryu D,Cantó C,Mottis A,Jo YS,Viswanathan M,Schoonjans K,Guarente L,Auwerx J

    更新日期:2013-07-18 00:00:00

  • A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells.

    abstract::We have identified a novel octamer binding factor (Oct-3) in P19 embryonal carcinoma cells. Oct-3, which recognizes the typical octamer motif (ATTTGCAT) as well as the AT-rich sequence TTAAAATTCA, is present in P19 stem cells but disappears when the cells are induced to differentiate by retinoic acid (RA). Cloned cDNA...


    pub_type: 杂志文章


    authors: Okamoto K,Okazawa H,Okuda A,Sakai M,Muramatsu M,Hamada H

    更新日期:1990-02-09 00:00:00

  • Influenza virus hemagglutinin expression is polarized in cells infected with recombinant SV40 viruses carrying cloned hemagglutinin DNA.

    abstract::Primary cell cultures of African Green monkey kidney (AGMK) contain polarized epithelial cells in which influenza virus matures predominantly at the apical surfaces above tight junctions. Influenza virus glycoproteins were found to be localized at the same membrane domain from which the virus budded. When polarized pr...


    pub_type: 杂志文章


    authors: Roth MG,Compans RW,Giusti L,Davis AR,Nayak DP,Gething MJ,Sambrook J

    更新日期:1983-06-01 00:00:00

  • Adaptive immunity to SARS-CoV-2 and COVID-19.

    abstract::The adaptive immune system is important for control of most viral infections. The three fundamental components of the adaptive immune system are B cells (the source of antibodies), CD4+ T cells, and CD8+ T cells. The armamentarium of B cells, CD4+ T cells, and CD8+ T cells has differing roles in different viral infect...


    pub_type: 杂志文章,评审


    authors: Sette A,Crotty S

    更新日期:2021-01-12 00:00:00

  • Another view of the selective model of thymocyte selection.

    abstract::Thymocyte commitment to the CD4 helper versus CD8 cytotoxic lineage has not been satisfactorily established. Two models have been elaborated: one based on instruction, the other on selection. Most previous results support the instructive model, but our comparison of thymocyte differentiation in MHC class II-, class I-...


    pub_type: 杂志文章


    authors: Chan SH,Cosgrove D,Waltzinger C,Benoist C,Mathis D

    更新日期:1993-04-23 00:00:00

  • Autophagy: paying Charon's toll.

    abstract::Autophagy (literally self-eating) has diverse cytoprotective functions but also is linked to programmed cell death (PCD). In this issue, Qu et al. (2007) analyze the role of autophagy in PCD during development. Unexpectedly they find that autophagy induces apoptotic cells to present a signal to ensure their clearance,...


    pub_type: 评论,杂志文章,评审


    authors: Yoshimori T

    更新日期:2007-03-09 00:00:00

  • Cryptic and polar variation of the fragile X repeat could result in predisposing normal alleles.

    abstract::Fragile X syndrome results from the expansion of a CGG repeat that is normally interrupted by occasional AGG triplets. Linkage disequilibrium suggests that certain normal haplotypes may contribute unequally to the pool of fragile X chromosomes. Sequence analysis of normal alleles demonstrates haplotype-specific variat...


    pub_type: 杂志文章


    authors: Kunst CB,Warren ST

    更新日期:1994-06-17 00:00:00

  • Optimal Decoding of Cellular Identities in a Genetic Network.

    abstract::In developing organisms, spatially prescribed cell identities are thought to be determined by the expression levels of multiple genes. Quantitative tests of this idea, however, require a theoretical framework capable of exposing the rules and precision of cell specification over developmental time. We use the gap gene...


    pub_type: 杂志文章


    authors: Petkova MD,Tkačik G,Bialek W,Wieschaus EF,Gregor T

    更新日期:2019-02-07 00:00:00

  • Modeling human nutrition using human embryonic stem cells.

    abstract::Nutrition presents unanswered scientific questions of high public health importance. We envision model systems composed of interacting gastrointestinal and metabolic tissues derived from human embryonic stem cells, populated by gut microbiota. The culture will be embedded in 3D scaffolds, creating a controlled experim...


    pub_type: 杂志文章


    authors: Ben-Zvi D,Melton DA

    更新日期:2015-03-26 00:00:00

  • The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1 beta-converting enzyme.

    abstract::We have cloned the C. elegans cell death gene ced-3. A ced-3 transcript is most abundant during embryogenesis, the stage during which most programmed cell deaths occur. The predicted CED-3 protein shows similarity to human and murine interleukin-1 beta-converting enzyme and to the product of the mouse nedd-2 gene, whi...


    pub_type: 杂志文章


    authors: Yuan J,Shaham S,Ledoux S,Ellis HM,Horvitz HR

    更新日期:1993-11-19 00:00:00

  • Changes in calmodulin and its mRNA accompany reentry of quiescent (G0) cells into the cell cycle.

    abstract::Release of CHO-K1 cells from plateau or stationary phase and reentry into the cell cycle is specifically and reversibly blocked at two distinct sites by the anticalmodulin drug W13. The first block occurs early during release while the cells are still at G0/G1, whereas the second occurs later in reentry during early S...


    pub_type: 杂志文章


    authors: Chafouleas JG,Lagacé L,Bolton WE,Boyd AE 3rd,Means AR

    更新日期:1984-01-01 00:00:00

  • Degradation of mRNA in eukaryotes.

    abstract::Based on the above mechanisms of mRNA degradation, an integrated model of mRNA turnover can be proposed (Figure 1). In this model, all polyadenylated mRNAs would be degraded by the deadenylation-dependent pathway at some rate. In addition to this default pathway, another layer of complexity would come from degradation...


    pub_type: 杂志文章,评审


    authors: Beelman CA,Parker R

    更新日期:1995-04-21 00:00:00

  • Distinct Ventral Pallidal Neural Populations Mediate Separate Symptoms of Depression.

    abstract::Major depressive disorder (MDD) patients display a common but often variable set of symptoms making successful, sustained treatment difficult to achieve. Separate depressive symptoms may be encoded by differential changes in distinct circuits in the brain, yet how discrete circuits underlie behavioral subsets of depre...


    pub_type: 杂志文章


    authors: Knowland D,Lilascharoen V,Pacia CP,Shin S,Wang EH,Lim BK

    更新日期:2017-07-13 00:00:00

  • Autocrine VEGF signaling synergizes with EGFR in tumor cells to promote epithelial cancer development.

    abstract::It is established that tumor cell-derived VEGF acts on endothelial cells to promote angiogenesis and tumor growth. Here, we demonstrate that in K5-SOS-dependent mouse skin tumors, autocrine VEGF is required for tumor cell proliferation in a cell-autonomous and angiogenesis-independent manner. VEGF is upregulated in SO...


    pub_type: 杂志文章


    authors: Lichtenberger BM,Tan PK,Niederleithner H,Ferrara N,Petzelbauer P,Sibilia M

    更新日期:2010-01-22 00:00:00

  • Travel Surveillance and Genomics Uncover a Hidden Zika Outbreak during the Waning Epidemic.

    abstract::The Zika epidemic in the Americas has challenged surveillance and control. As the epidemic appears to be waning, it is unclear whether transmission is still ongoing, which is exacerbated by discrepancies in reporting. To uncover locations with lingering outbreaks, we investigated travel-associated Zika cases to identi...


    pub_type: 杂志文章


    authors: Grubaugh ND,Saraf S,Gangavarapu K,Watts A,Tan AL,Oidtman RJ,Ladner JT,Oliveira G,Matteson NL,Kraemer MUG,Vogels CBF,Hentoff A,Bhatia D,Stanek D,Scott B,Landis V,Stryker I,Cone MR,Kopp EW 4th,Cannons AC,Heberlein-L

    更新日期:2019-08-22 00:00:00

  • Zip2, a meiosis-specific protein required for the initiation of chromosome synapsis.

    abstract::We describe the identification and characterization of the Saccharomyces cerevisiae ZIP2 gene, which encodes a novel meiosis-specific protein essential for synaptonemal complex formation. In the zip2 mutant, chromosomes are homologously paired but not synapsed. The Zip2 protein localizes to discrete foci on meiotic ch...


    pub_type: 杂志文章


    authors: Chua PR,Roeder GS

    更新日期:1998-05-01 00:00:00

  • Isolation of two genes that affect mitotic chromosome transmission in S. cerevisiae.

    abstract::Two DNA sequences that reduce mitotic fidelity of chromosome transmission have been identified: MIF1 and MIF2. MIF1 is a unique sequence located on the right arm of chromosome XII that stimulates loss and recombination for both chromosomes V and VII when present in a high copy number plasmid. MIF1 is not essential for...


    pub_type: 杂志文章


    authors: Meeks-Wagner D,Wood JS,Garvik B,Hartwell LH

    更新日期:1986-01-17 00:00:00

  • Circadian gene expression in individual fibroblasts: cell-autonomous and self-sustained oscillators pass time to daughter cells.

    abstract::The mammalian circadian timing system is composed of a central pacemaker in the suprachiasmatic nucleus (SCN) of the brain and subsidiary oscillators in most peripheral cell types. While oscillators in SCN neurons are known to function in a self-sustained fashion, peripheral oscillators have been thought to damp rapid...


    pub_type: 杂志文章


    authors: Nagoshi E,Saini C,Bauer C,Laroche T,Naef F,Schibler U

    更新日期:2004-11-24 00:00:00

  • Export of proteins from oocytes of Xenopus laevis.

    abstract::When human lymphoblastoid mRNA was microinjected into X. laevis oocytes, titers of interferon rapidly reached a maximum inside the oocyte while accumulation of interferon continued in the incubation medium for at least 45 hr. If interferon protein was injected into oocytes it was rapidly inactivated. Significantly, ne...


    pub_type: 杂志文章


    authors: Colman A,Morser J

    更新日期:1979-07-01 00:00:00

  • Spatially restricted expression of pipe in the Drosophila egg chamber defines embryonic dorsal-ventral polarity.

    abstract::Expression of pipe in the somatic tissue of the Drosophila ovary is required for the formation of embryonic dorsal-ventral polarity. pipe, which encodes an enzyme similar to the glycosaminoglycan-modifying enzyme heparan sulfate 2-O-sulfotransferase, is expressed in a spatially restricted domain of follicle cells on t...


    pub_type: 杂志文章


    authors: Sen J,Goltz JS,Stevens L,Stein D

    更新日期:1998-11-13 00:00:00

  • Structural features of the GroEL-GroES nano-cage required for rapid folding of encapsulated protein.

    abstract::GroEL and GroES form a chaperonin nano-cage for proteins up to approximately 60 kDa to fold in isolation. Here we explored the structural features of the chaperonin cage critical for rapid folding of encapsulated substrates. Modulating the volume of the GroEL central cavity affected folding speed in accordance with co...


    pub_type: 杂志文章


    authors: Tang YC,Chang HC,Roeben A,Wischnewski D,Wischnewski N,Kerner MJ,Hartl FU,Hayer-Hartl M

    更新日期:2006-06-02 00:00:00