Abstract:
:Ku is a heterodimeric DNA end binding complex composed of 70 and 86 kDa subunits. Here, we show that Ku86 is essential for normal V(D)J recombination in vivo, as Ku86-deficient mice are severely defective for formation of coding joints. Unlike severe combined immunodeficient (scid) mice, Ku86-deficient mice are also defective for signal joint formation. Both hairpin coding ends and blunt full-length signal ends accumulate. Contrary to expectation, Ku86 is evidently not required for protection of either type of V(D)J recombination intermediate. Instead, V(D)J recombination appears to be arrested after the cleavage step in Ku86-deficient mice. We suggest that Ku86 may be required to remodel or disassemble DNA-protein complexes containing broken ends, making them available for further processing and joining.
journal_name
Celljournal_title
Cellauthors
Zhu C,Bogue MA,Lim DS,Hasty P,Roth DBdoi
10.1016/s0092-8674(00)80111-7subject
Has Abstractpub_date
1996-08-09 00:00:00pages
379-89issue
3eissn
0092-8674issn
1097-4172pii
S0092-8674(00)80111-7journal_volume
86pub_type
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