Abstract：
:Cis-regulatory elements (CREs) are commonly recognized by correlative chromatin features, yet the molecular composition of the vast majority of CREs in chromatin remains unknown. Here, we describe a CRISPR affinity purification in situ of regulatory elements (CAPTURE) approach to unbiasedly identify locus-specific chromatin-regulating protein complexes and long-range DNA interactions. Using an in vivo biotinylated nuclease-deficient Cas9 protein and sequence-specific guide RNAs, we show high-resolution and selective isolation of chromatin interactions at a single-copy genomic locus. Purification of human telomeres using CAPTURE identifies known and new telomeric factors. In situ capture of individual constituents of the enhancer cluster controlling human β-globin genes establishes evidence for composition-based hierarchical organization. Furthermore, unbiased analysis of chromatin interactions at disease-associated cis-elements and developmentally regulated super-enhancers reveals spatial features that causally control gene transcription. Thus, comprehensive and unbiased analysis of locus-specific regulatory composition provides mechanistic insight into genome structure and function in development and disease.
journal_name
Celljournal_title
Cellauthors
Liu X,Zhang Y,Chen Y,Li M,Zhou F,Li K,Cao H,Ni M,Liu Y,Gu Z,Dickerson KE,Xie S,Hon GC,Xuan Z,Zhang MQ,Shao Z,Xu Jdoi
10.1016/j.cell.2017.08.003subject
Has Abstractpub_date
2017-08-24 00:00:00pages
1028-1043.e19issue
5eissn
0092-8674issn
1097-4172pii
S0092-8674(17)30891-7journal_volume
170pub_type
杂志文章相关文献
CELL文献大全abstract：:X-ray studies have established that the structure of a right-handed, Watson-Crick double helix can change from place to place along its length as a function of base sequence. The base pairs transmit deformations out to the phosphate backbone, where they can then be recognized by proteins and other DNA-binding reagents...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(84)90379-9
更新日期：1984-06-01 00:00:00
abstract：:We have fused DNA fragments derived from an Escherichia coli tRNALeu1 operon to the galK gene of E. coli to identify sequences necessary for the in vivo initiation of transcription and growth-rate-dependent regulation. Promoter sequences consisting of residues from -50 to +56 or -50 to +5 with respect to the in vivo s...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(82)90290-2
更新日期：1982-10-01 00:00:00
abstract：:The transcription factor ATF2 elicits oncogenic activities in melanoma and tumor suppressor activities in nonmalignant skin cancer. Here, we identify that ATF2 tumor suppressor function is determined by its ability to localize at the mitochondria, where it alters membrane permeability following genotoxic stress. The a...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2012.01.016
更新日期：2012-02-03 00:00:00
abstract：:We have identified an essential gene, called FIP1, encoding a 327 amino acid protein interacting with yeast poly(A) polymerase (PAP1) in the two-hybrid assay. Recombinant FIP1 protein forms a 1:1 complex with PAP1 in vitro. At 37 degrees C, a thermosensitive allele of FIP1 shows a shortening of poly(A) tails and a dec...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90391-7
更新日期：1995-05-05 00:00:00
abstract：:Spinal muscular atrophy (SMA) is a lethal human disease characterized by motor neuron dysfunction and muscle deterioration due to depletion of the ubiquitous survival motor neuron (SMN) protein. Drosophila SMN mutants have reduced muscle size and defective locomotion, motor rhythm, and motor neuron neurotransmission. ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2012.09.011
更新日期：2012-10-12 00:00:00
abstract：:We have determined the nucleotide sequences of the germ line gene as well as a corresponding somatically mutated and rearranged gene coding for a mouse immunoglobulin lambdaI type light chain. These sequencing studies were carried out on three Eco RI-DNA fragments which had been cloned from BALB/c mouse embryos or a l...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(78)90041-7
更新日期：1978-12-01 00:00:00
abstract：:Linear simian virus 40 (SV40) chromosomes were prepared by Eco R1 nuclease cleavage of the circular SV40 chromosomes released from virions with dithiothreitol at pH 9,8. Chromatin-DNA hybrids were constructed with segments of 3H-labeled, naked SV40 DNA covalently joined via the Eco R1-generated cohesive ends to segmen...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(78)90279-9
更新日期：1978-11-01 00:00:00
abstract：:Viable yet damaged cells can accumulate during development and aging. Although eliminating those cells may benefit organ function, identification of this less fit cell population remains challenging. Previously, we identified a molecular mechanism, based on "fitness fingerprints" displayed on cell membranes, which all...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.12.017
更新日期：2015-01-29 00:00:00
abstract：:The modularity of protein architecture and the diversity of protein domains hint at a vast combinatorial richness. But evolution appears to have been relatively conservative about selecting new combinations. When a particular grouping of domains within a polypeptide chain can perform a concerted function, that combina...
journal_title：Cell
pub_type： 评论,杂志文章,评审
doi：10.1016/s0092-8674(03)00196-x
更新日期：2003-03-21 00:00:00
abstract：:Phosphorylation of histone H3 serine 10 correlates with chromosome condensation and is required for normal chromosome segregation in Tetrahymena. This phosphorylation is dependent upon activation of the NIMA kinase in Aspergillus nidulans. NIMA expression also induces Ser-10 phosphorylation inappropriately in S phase-...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)00035-0
更新日期：2000-08-04 00:00:00
abstract：:During the early stage of Ad2 infection of human cells, RNA is transcribed from five separate transcription units. Early region II encodes the mRNA for a 72K single-stranded DNA binding protein (DBP) which functions in DNA replication. This report describes the structure of the first leader of the DBP mRNA and the fla...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(79)90073-4
更新日期：1979-10-01 00:00:00
abstract：:In Xenopus development, the expression of several maternal mRNAs is regulated by cytoplasmic polyadenylation. CPEB and maskin, two factors that control polyadenylation-induced translation are present on the mitotic apparatus of animal pole blastomeres in embryos. Cyclin B1 protein and mRNA, whose translation is regula...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)00135-5
更新日期：2000-10-27 00:00:00
abstract：:Six restriction fragments of Ad2 DNA which contain sites for RNA initiation have been identified by their ability to hybridize nascent labeled RNA less than 1 kb in length. Four RNA initiation sites early in infection are identified in regions where previous work (Flint, 1977) had mapped mRNAs. The major late RNA init...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(77)90273-2
更新日期：1977-11-01 00:00:00
abstract：:After translational termination, mRNA and P site deacylated tRNA remain associated with ribosomes in posttermination complexes (post-TCs), which must therefore be recycled by releasing mRNA and deacylated tRNA and by dissociating ribosomes into subunits. Recycling of bacterial post-TCs requires elongation factor EF-G ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2007.08.041
更新日期：2007-10-19 00:00:00
abstract：:In this issue, Metzis et al., demonstrate that in the development of the central nervous system, patterning along the anterior-posterior axis precedes acquisition of neural identity. This contrasts with the prevailing view that neural identity comes first, providing a new window on the origins of the brain and spinal ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2018.10.040
更新日期：2018-11-01 00:00:00
abstract：:Nuclear receptors undergo ligand-dependent conformational changes that are required for corepressor-coactivator exchange, but whether there is an actual requirement for specific epigenetic landmarks to impose ligand dependency for gene activation remains unknown. Here we report an unexpected and general strategy that ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2006.12.038
更新日期：2007-02-09 00:00:00
abstract：:ABCA1, an ATP-binding cassette (ABC) subfamily A exporter, mediates the cellular efflux of phospholipids and cholesterol to the extracellular acceptor apolipoprotein A-I (apoA-I) for generation of nascent high-density lipoprotein (HDL). Mutations of human ABCA1 are associated with Tangier disease and familial HDL defi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.05.020
更新日期：2017-06-15 00:00:00
abstract：:We have isolated and characterized a set of overlapping cDNA clones that encode the human centromere autoantigen centromere protein C (CENP-C). The identity of these clones has been established using several criteria. First, they were shown to encode a polypeptide that migrates at the expected position for CENP-C on S...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90538-n
更新日期：1992-07-10 00:00:00
abstract：:The role of S. cerevisiae YDJ1 protein (YDJ1p) in polypeptide translocation across membranes has been examined. A conditional ydj1 mutant strain (ydj1-151TS) is defective for import of several polypeptides into mitochondria and alpha factor into the endoplasmic reticulum at 37 degrees C. These defects are suppressed b...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(05)80063-7
更新日期：1992-12-24 00:00:00
abstract：:In mouse intestinal tumors induced by the inhibition of APC, the restoration of APC function causes complete tumor regression with normal differentiation and return of stem cell function irrespective of whether tumors also carried mutations in Kras and p53. These findings by Dow et al. validate the Wnt pathway as an e...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2015.06.008
更新日期：2015-06-18 00:00:00
abstract：:Is the Café Scientifique a fashionable by-product of a comfortable age or an indicator of the changing relationship between science and society? ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2006.07.006
更新日期：2006-07-28 00:00:00
abstract：:Twitter needs no introduction. This "microblogging" service has gained tremendous popularity in the 2 years since its launch. Yet, most scientists are steering clear of it. Laura Bonetta speaks to some who have found value in tweeting. ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2009.10.017
更新日期：2009-10-30 00:00:00
abstract：:The FoxO transcription factors have been implicated in many processes including tumor suppression and cell death. In this issue, two groups now report on mice that conditionally lack the three predominant FoxO transcription factors. Demonstrate that FoxOs are critical for the long-term maintenance of hematopoietic ste...
journal_title：Cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.cell.2007.01.009
更新日期：2007-01-26 00:00:00
abstract：:Enteric-associated neurons (EANs) are closely associated with immune cells and continuously monitor and modulate homeostatic intestinal functions, including motility and nutrient sensing. Bidirectional interactions between neuronal and immune cells are altered during disease processes such as neurodegeneration or irri...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.12.002
更新日期：2020-01-09 00:00:00
abstract：:Mitochondrial DNA is compacted into nucleoprotein complexes denoted mitochondrial nucleoids, the focus of this SnapShot. ...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2017.12.039
更新日期：2018-01-11 00:00:00
abstract：:Even a simple sensory stimulus can elicit distinct innate behaviors and sequences. During sensorimotor decisions, competitive interactions among neurons that promote distinct behaviors must ensure the selection and maintenance of one behavior, while suppressing others. The circuit implementation of these competitive i...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.09.009
更新日期：2016-10-20 00:00:00
abstract：:The functional consequences of glycan structural changes associated with cellular differentiation are ill defined. Herein, we investigate the role of glycan adducts to the O-glycosylated polypeptide stalk tethering the CD8alphabeta coreceptor to the thymocyte surface. We show that immature CD4(+)CD8(+) double-positive...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(01)00577-3
更新日期：2001-11-16 00:00:00
abstract：:Receptor-interacting protein kinase (RIPK)-1 is involved in RIPK3-dependent and -independent signaling pathways leading to cell death and/or inflammation. Genetic ablation of ripk1 causes postnatal lethality, which was not prevented by deletion of ripk3, caspase-8, or fadd. However, animals that lack RIPK1, RIPK3, and...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.04.018
更新日期：2014-05-22 00:00:00
abstract：:Cancer cells have distinctive nutrient demands to fuel growth and proliferation, including the disproportionate use of glucose, glutamine, and fatty acids. Comerford et al. and Mashimo et al. now demonstrate that several types of cancer are avid consumers of acetate, which facilitates macromolecular biosynthesis and h...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.12.009
更新日期：2014-12-18 00:00:00
abstract：:The brightness of our visual environment varies tremendously from day to night. In this issue of Cell, Milner and Do describe how the population of retinal neurons responsible for entrainment of the brain's circadian clock cooperate to encode irradiance across a wide range of ambient-light intensities. ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2017.10.028
更新日期：2017-11-02 00:00:00
