Abstract：
:A family of novel substrates was designed to enable the efficient accumulation of intermediates in site-specific recombination. Strategically placed nicks allow these "suicide substrates" to initiate the reaction but prevent its completion or reversal. Consequently, it has been possible to determine that lambda site-specific recombination proceeds by a pair of sequential single-strand exchanges. These results rule out that class of models invoking a concerted cutting of all four DNA strands. The sequential strand exchanges are executed in a strictly prescribed order that is the same in both integrative and excisive recombination. This specified order appears to be governed by the arrangement of bound proteins distal to the sites of strand exchange. Furthermore, when provided with an appropriate 5' OH acceptor, the Integrase protein has the capacity to execute a single DNA strand transfer in a nonreciprocal reaction.
journal_name
Celljournal_title
Cellauthors
Nunes-Düby SE,Matsumoto L,Landy Adoi
10.1016/0092-8674(87)90336-9subject
Has Abstractpub_date
1987-08-28 00:00:00pages
779-88issue
5eissn
0092-8674issn
1097-4172pii
0092-8674(87)90336-9journal_volume
50pub_type
杂志文章相关文献
CELL文献大全abstract：:To relate different forms of synaptic plasticity to the formation and maintenance of place cells in the hippocampus, we have recorded place cells in freely behaving, transgenic mice that express a mutated Ca2+-independent form of CaM Kinase II. These mice have normal long-term potentiation (LTP) at 100 Hz, but they la...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81829-2
更新日期：1996-12-27 00:00:00
abstract：:The replication initiator protein of the plasmid R6K binds to seven contiguous 22 bp direct repeats that form an indispensable part of the three replication origins alpha, beta, and gamma. Binding of the initiator to the direct repeats induced a marked bending of the region of gamma replication origin. Binding of the ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(85)90023-6
更新日期：1985-11-01 00:00:00
abstract：:One of the important events in synapse formation is the accumulation of neurotransmitter receptors beneath the presynaptic nerve terminal. Agrin is a component of the synaptic basal lamina that induces the clustering of acetylcholine receptors when bath-applied to muscle fibers in culture. When a cDNA encoding a putat...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90364-5
更新日期：1991-11-29 00:00:00
abstract：:The Arabidopsis floral homeotic gene AGAMOUS (AG) is required for development of the reproductive organs (stamens and carpels). In ag mutants, the loss of AG function leads to the conversion of these organs to the perianth organs (petals and sepals). In contrast, mutations in another floral homeotic gene, APETALA2 (AP...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90271-d
更新日期：1992-10-02 00:00:00
abstract：:Cancer cell of origin is difficult to identify by analyzing cells within terminal stage tumors, whose identity could be concealed by the acquired plasticity. Thus, an ideal approach to identify the cell of origin is to analyze proliferative abnormalities in distinct lineages prior to malignancy. Here, we use mosaic an...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2011.06.014
更新日期：2011-07-22 00:00:00
abstract：:We chemically synthesized 20 peptides corresponding to 75% of the HA1 molecule of the influenza virus. Antibodies to the majority (18) of these peptides were capable of reacting with the hemagglutinin molecule. These 18 peptides are not confined to the known antigenic determinants of the hemagglutinin molecule, but ra...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(82)90202-1
更新日期：1982-03-01 00:00:00
abstract：:Inducer T-cell clones reactive to the p-azobenzenearsonate (arsonate) hapten possess binding sites for radioactive arsanylated proteins, which are not present on clones with other antigen specificities. Binding occurred in the absence of histocompatibility proteins. Binding was specific for the p-azobenzenearsonate ha...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(84)90037-0
更新日期：1984-04-01 00:00:00
abstract：:Protein-protein interaction maps provide a valuable framework for a better understanding of the functional organization of the proteome. To detect interacting pairs of human proteins systematically, a protein matrix of 4456 baits and 5632 preys was screened by automated yeast two-hybrid (Y2H) interaction mating. We id...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2005.08.029
更新日期：2005-09-23 00:00:00
abstract：:A 57 kd component of oligosaccharyl transferase, termed glycosylation site binding protein, specifically recognizes a photoaffinity probe containing the N-glycosylation site sequence Asn-Lys-Thr. It is present in the lumen of the ER (endoplasmic reticulum) and its release from this compartment results in a loss of N-g...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(88)90120-1
更新日期：1988-09-23 00:00:00
abstract：:Many proteins (intrinsically disordered proteins, IDPs) or regions of proteins (intrinsically disordered regions, IDRs) lack a well-defined 3D structure under physiological conditions. Albeit unfolded and highly dynamic, these proteins are not denatured; rather, intrinsic structural disorder is their native, functiona...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.05.024
更新日期：2015-05-21 00:00:00
abstract：:Alterations of symbiosis between microbiota and intestinal epithelial cells (IEC) are associated with intestinal and systemic pathologies. Interactions between bacterial products (MAMPs) and Toll-like receptors (TLRs) are known to be mandatory for IEC homeostasis, but how TLRs may time homeostatic functions with circa...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.04.020
更新日期：2013-05-09 00:00:00
abstract：:The protein Par-4 acts in the cytoplasm to trigger cell death signaling via caspase activation and the mitochondrial release of cytochrome c. Burikhanov et al. (2009) now provide surprising evidence that Par-4 can also promote apoptosis from outside the cell, after its secretion in response to endoplasmic reticulum st...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2009.07.007
更新日期：2009-07-23 00:00:00
abstract：:Accumulating evidence for nascent-peptide-mediated regulation of translation suggests that all nascent peptides do not necessarily interact with the ribosome in a similar manner. Recent studies have helped to elucidate the exit route of the nascent chain and its interactions with the ribosome. ...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/s0092-8674(02)00669-4
更新日期：2002-03-08 00:00:00
abstract：:RNA-guided Cas9 endonucleases protect bacteria from viral infection and have been creatively repurposed as programmable molecular scalpels for surgical manipulation of DNA. Now, two papers in Cell (Pawluk et al. and Rauch et al.) identify viral proteins that suppress Cas9 and may function like molecular sheaths for th...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2016.12.007
更新日期：2017-01-12 00:00:00
abstract：:The minimal DNA sequence required for the formation of a stable complex with Drosophila heat shock factor (HSF) in vitro is an inverted repeat of a 5 bp recognition unit, -GAA-. Surprisingly, both permutations of this 5 bp unit, head-to-head and tail-to-tail, bind to HSF with similar affinity and with striking 2-fold ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(89)90603-x
更新日期：1989-12-01 00:00:00
abstract：:We have isolated the Drosophila melanogaster homolog (Dint-1) of int-1, a conserved cellular oncogene implicated in viral mammary tumorigenesis in mice. The deduced Dint-1 protein sequence contains 468 amino acids and starts with a hydrophobic leader; it is 54% identical to the int-1 sequence, and all 23 cysteine resi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(87)90038-9
更新日期：1987-08-14 00:00:00
abstract：:To elucidate the evolutionary origin of nervous system centralization, we investigated the molecular architecture of the trunk nervous system in the annelid Platynereis dumerilii. Annelids belong to Bilateria, an evolutionary lineage of bilateral animals that also includes vertebrates and insects. Comparing nervous sy...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2007.02.040
更新日期：2007-04-20 00:00:00
abstract：:Metastasis is the leading cause of cancer-associated death but has been difficult to study because it involves a series of rare, stochastic events. To capture these events, we developed a sensitive method to tag and track pancreatic epithelial cells in a mouse model of pancreatic cancer. Tagged cells invaded and enter...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2011.11.025
更新日期：2012-01-20 00:00:00
abstract：:There is tremendous enthusiasm for the microbiome in academia and industry. This Perspective argues that in order to realize its potential, the field needs to focus on establishing causation and molecular mechanism with an emphasis on phenotypes that are large in magnitude, easy to measure, and unambiguously driven by...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2018.07.038
更新日期：2018-08-09 00:00:00
abstract：:During HIV infection, the timing of opportunistic infections is not always associated with severity of CD4 T cell depletion, and different opportunistic pathogens reactivate at different CD4 T cell thresholds. Here, we examine how differences in the phenotype and function of pathogen-specific CD4 T cells influence sus...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.09.045
更新日期：2013-10-24 00:00:00
abstract：:Transcriptional regulation by the glucocorticoid receptor (GR) is mediated by hormone binding, receptor dimerization, and coactivator recruitment. Here, we report the crystal structure of the human GR ligand binding domain (LBD) bound to dexamethasone and a coactivator motif derived from the transcriptional intermedia...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(02)00817-6
更新日期：2002-07-12 00:00:00
abstract：:Abiotic stress, such as salinity, drought, and cold, causes detrimental yield losses for all major plant crop species. Understanding mechanisms that improve plants' ability to produce biomass, which largely is constituted by the plant cell wall, is therefore of upmost importance for agricultural activities. Cellulose ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.08.028
更新日期：2015-09-10 00:00:00
abstract：:We have characterized an aberrant allele of a variably spliced Drosophila tropomyosin gene. The allele was recovered from the flightless Ifm(3)3 strain, which has been shown to have structurally and functionally abnormal indirect flight muscles. The transcribed portion of the mutant gene is interrupted by an 8,8 kb in...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(85)90061-3
更新日期：1985-05-01 00:00:00
abstract：:In this issue of Cell, identify a first regulatory link between planar cell polarity (PCP) signaling and apical-basal polarity. The authors propose that a component of the apical Crumbs complex regulates the phosphorylation of the Frizzled (Fz) PCP receptor, thus modulating PCP in the Drosophila eye. ...
journal_title：Cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.cell.2005.05.006
更新日期：2005-05-20 00:00:00
abstract：:In the mouse, the zona pellucida (ZP) glycoprotein ZP3 both binds intact sperm and induces acrosomal exocytosis. The subsequent signaling pathway(s) is still uncertain, but Gi-like proteins have been implicated. By analogy with other signal transduction mechanisms, we examined anti-phosphotyrosine antibody reactivity ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(89)90049-4
更新日期：1989-06-30 00:00:00
abstract：:Forms of RNA polymerase I prepared from growing or encysted Acanthamoeba are equal in the ability to transcribe poly(dl:dC). Polymerase from cysts, whose rRNA genes are transcriptionally inactive, is unable to utilize the rDNA promoter in vitro, whereas the transcription initiation factor from cysts is fully able to b...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(86)90601-x
更新日期：1986-11-07 00:00:00
abstract：:In embryonal carcinoma (EC) cells retinoic acid (RA) strongly induces transcription from the RA receptor beta 2 (RAR beta 2) promoter through an RA response element (RARE) located in close proximity to the TATA box. Here we demonstrate that recombinant human TATA box-binding protein, hTFIID, and RAR functionally coope...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90443-g
更新日期：1992-05-01 00:00:00
abstract：:The phenotype of the autosomal recessive mutation scat includes severe intermittent bleeding, depletion of platelets, and circulating anti-platelet antibodies. In this study, we have mapped the scat mutation to mouse chromosome 8 and shown that the immune component is a secondary consequence of the gene defect. Surpri...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(93)90301-6
更新日期：1993-07-16 00:00:00
abstract：:Aging has been associated with a progressive decline of proteostasis, but how this process affects proteome composition remains largely unexplored. Here, we profiled more than 5,000 proteins along the lifespan of the nematode C. elegans. We find that one-third of proteins change in abundance at least 2-fold during agi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.03.032
更新日期：2015-05-07 00:00:00
abstract：:Retrograde transport, in which proteins and lipids are shuttled between endosomes and biosynthetic/secretory compartments such as the Golgi apparatus, is crucial for a diverse range of cellular functions. Mechanistic studies that explore the molecular machinery involved in this retrograde trafficking route are sheddin...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2008.12.009
更新日期：2008-12-26 00:00:00
