Abstract:
:The replication initiator protein of the plasmid R6K binds to seven contiguous 22 bp direct repeats that form an indispensable part of the three replication origins alpha, beta, and gamma. Binding of the initiator to the direct repeats induced a marked bending of the region of gamma replication origin. Binding of the initiator also promoted unwinding of the origin DNA by at least two turns. Distamycin appeared to antagonize the binding of the initiator to the seven 22 bp direct repeats. At the appropriate DNA and protein concentrations the initiator enhanced topoisomerase-induced catenation of the origin containing supercoiled DNA but not of DNA lacking the origin sequence. Thus, the initiator protein caused significant changes in the secondary and tertiary structures of the replication origin.
journal_name
Celljournal_title
Cellauthors
Mukherjee S,Patel I,Bastia Ddoi
10.1016/0092-8674(85)90023-6subject
Has Abstractpub_date
1985-11-01 00:00:00pages
189-97issue
1eissn
0092-8674issn
1097-4172pii
0092-8674(85)90023-6journal_volume
43pub_type
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