Conformational changes in a replication origin induced by an initiator protein.

Abstract:

:The replication initiator protein of the plasmid R6K binds to seven contiguous 22 bp direct repeats that form an indispensable part of the three replication origins alpha, beta, and gamma. Binding of the initiator to the direct repeats induced a marked bending of the region of gamma replication origin. Binding of the initiator also promoted unwinding of the origin DNA by at least two turns. Distamycin appeared to antagonize the binding of the initiator to the seven 22 bp direct repeats. At the appropriate DNA and protein concentrations the initiator enhanced topoisomerase-induced catenation of the origin containing supercoiled DNA but not of DNA lacking the origin sequence. Thus, the initiator protein caused significant changes in the secondary and tertiary structures of the replication origin.

journal_name

Cell

journal_title

Cell

authors

Mukherjee S,Patel I,Bastia D

doi

10.1016/0092-8674(85)90023-6

subject

Has Abstract

pub_date

1985-11-01 00:00:00

pages

189-97

issue

1

eissn

0092-8674

issn

1097-4172

pii

0092-8674(85)90023-6

journal_volume

43

pub_type

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