Abstract:
:During eukaryotic translation initiation, initiator tRNA does not insert fully into the P decoding site on the 40S ribosomal subunit. This conformation (POUT) is compatible with scanning mRNA for the AUG start codon. Base pairing with AUG is thought to promote isomerization to a more stable conformation (PIN) that arrests scanning and promotes dissociation of eIF1 from the 40S subunit. Here, we present a cryoEM reconstruction of a yeast preinitiation complex at 4.0 Å resolution with initiator tRNA in the PIN state, prior to eIF1 release. The structure reveals stabilization of the codon-anticodon duplex by the N-terminal tail of eIF1A, changes in the structure of eIF1 likely instrumental in its subsequent release, and changes in the conformation of eIF2. The mRNA traverses the entire mRNA cleft and makes connections to the regulatory domain of eIF2?, eIF1A, and ribosomal elements that allow recognition of context nucleotides surrounding the AUG codon.
journal_name
Celljournal_title
Cellauthors
Hussain T,Llácer JL,Fernández IS,Munoz A,Martin-Marcos P,Savva CG,Lorsch JR,Hinnebusch AG,Ramakrishnan Vdoi
10.1016/j.cell.2014.10.001subject
Has Abstractpub_date
2014-10-23 00:00:00pages
597-607issue
3eissn
0092-8674issn
1097-4172pii
S0092-8674(14)01281-1journal_volume
159pub_type
杂志文章相关文献
CELL文献大全abstract::The integration of mass spectrometry-based proteomics with next-generation DNA and RNA sequencing profiles tumors more comprehensively. Here this "proteogenomics" approach was applied to 122 treatment-naive primary breast cancers accrued to preserve post-translational modifications, including protein phosphorylation a...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.10.036
更新日期:2020-11-25 00:00:00
abstract::c-Abl is a tyrosine kinase localized primarily in the nucleus. Previous assays for abl function rely on cellular transformation by abl mutants, which are cytoplasmic. Using a conditional overexpression strategy, we have developed a functional assay for c-abl. Overexpression of c-abl inhibits growth by causing cell cyc...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90240-2
更新日期:1994-04-08 00:00:00
abstract::In familial searching in forensic genetics, a query DNA profile is tested against a database to determine whether it represents a relative of a database entrant. We examine the potential for using linkage disequilibrium to identify pairs of profiles as belonging to relatives when the query and database rely on nonover...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.09.008
更新日期:2018-10-18 00:00:00
abstract::In an era marked by the increasing prevalence of obesity, diabetes, and cardiovascular disease, the nuclear receptor peroxisome proliferator-activated receptor gamma (PPARgamma) has emerged as a transcriptional regulator of metabolism whose activity can be modulated by direct binding of small molecules. As the master ...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2005.11.026
更新日期:2005-12-16 00:00:00
abstract::We describe a monoclonal antibody directed against a neuron-specific mitochondrial protein from rat brain. On protein blots the antibody recognizes a single polypeptide of apparent molecular weight of 23,000. By solid-phase immunoassay the antigen was detected in nonneural tissues. Within neurons, the antibody stains ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90343-9
更新日期:1982-02-01 00:00:00
abstract::Periodic transcription of yeast histone genes has been demonstrated by DNA excess filter hybridization of in vivo pulse-labeled RNA isolated from synchronous cell cultures. Using strains carrying cell division cycle (cdc) mutations, we show that both activation and termination of transcription are determined by tempor...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90036-8
更新日期:1982-08-01 00:00:00
abstract::The textbook view of gene activation is that the rate-limiting step is the interaction of RNA polymerase II (Pol II) with the gene's promoter. However, studies in a variety of systems, including human embryonic stem cells and the early Drosophila embryo, have begun to challenge this view. There is increasing evidence ...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2011.04.021
更新日期:2011-05-13 00:00:00
abstract::The V(D)J recombinase subunits Rag-1 and Rag-2 mediate assembly of antigen receptor gene segments. We studied the mechanisms of DNA recognition by Rag-1/Rag-2 using surface plasmon resonance. The critical step for signal recognition is binding of Rag-1 to the nonamer. This is achieved by a region of Rag-1 homologous t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81344-6
更新日期:1996-10-18 00:00:00
abstract::Iron is ubiquitous in the environment and in biology. The study of iron biology focuses on physiology and homeostasis-understanding how cells and organisms regulate their iron content, how diverse tissues orchestrate iron allocation, and how dysregulated iron homeostasis leads to common hematological, metabolic, and n...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/s0092-8674(04)00343-5
更新日期:2004-04-30 00:00:00
abstract::The recognition of specific DNA-binding sites by transcription factors is a critical yet poorly understood step in the control of gene expression. Members of the Hox family of transcription factors bind DNA by making nearly identical major groove contacts via the recognition helices of their homeodomains. In vivo spec...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.09.024
更新日期:2007-11-02 00:00:00
abstract::Atopic dermatitis (AD) is a chronic itch and inflammatory disorder of the skin that affects one in ten people. Patients suffering from severe AD eventually progress to develop asthma and allergic rhinitis, in a process known as the "atopic march." Signaling between epithelial cells and innate immune cells via the cyto...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.08.057
更新日期:2013-10-10 00:00:00
abstract::The initiation of chromosome segregation at anaphase is linked by the spindle assembly checkpoint to the completion of chromosome-microtubule attachment during metaphase. To determine the function of the mitotic checkpoint protein Mad2 during normal cell division and when mitosis goes awry, we have knocked out Mad2 in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80875-2
更新日期:2000-06-09 00:00:00
abstract::Synthesis of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], a signaling phospholipid, is primarily carried out by phosphatidylinositol 4-phosphate 5-kinase [PI(4)P5K], which has been reported to be regulated by RhoA and Rac1. Unexpectedly, we find that the GTPgammaS-dependent activator of PI(4)P5Kalpha is the smal...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81540-8
更新日期:1999-11-24 00:00:00
abstract::We have determined the complete 9202 nucleotide sequence of the visna lentivirus. The deduced genetic organization most closely resembles that of the AIDS retrovirus in that there is a novel central region separating pol and env. Moreover, there is a close phylogenetic relationship between the conserved reverse transc...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(85)80132-x
更新日期:1985-08-01 00:00:00
abstract::Release of CHO-K1 cells from plateau or stationary phase and reentry into the cell cycle is specifically and reversibly blocked at two distinct sites by the anticalmodulin drug W13. The first block occurs early during release while the cells are still at G0/G1, whereas the second occurs later in reentry during early S...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90075-8
更新日期:1984-01-01 00:00:00
abstract::Evidence is now mounting that liquid-liquid phase separation (LLPS) underlies the formation of membraneless compartments in cells. This realization has motivated major efforts to delineate the function of such biomolecular condensates in normal cells and their roles in contexts ranging from development to age-related ...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2018.12.035
更新日期:2019-01-24 00:00:00
abstract::The dual host range virus HIX has been previously characterized as an envelope gene recombinant between Moloney murine leukemia virus (Mo-MuLV) and an unidentified xenotropic murine leukemia virus. Using long reverse transcripts of Mo-MuLV, a region of nonhomology has been mapped by electron microscopic analysis of he...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90350-1
更新日期:1978-08-01 00:00:00
abstract::The phenotype of the autosomal recessive mutation scat includes severe intermittent bleeding, depletion of platelets, and circulating anti-platelet antibodies. In this study, we have mapped the scat mutation to mouse chromosome 8 and shown that the immune component is a secondary consequence of the gene defect. Surpri...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90301-6
更新日期:1993-07-16 00:00:00
abstract::The heterogeneity of small extracellular vesicles and presence of non-vesicular extracellular matter have led to debate about contents and functional properties of exosomes. Here, we employ high-resolution density gradient fractionation and direct immunoaffinity capture to precisely characterize the RNA, DNA, and prot...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.02.029
更新日期:2019-04-04 00:00:00
abstract::Nucleotide sequences in two wild-type and six mutant operators in the DNA of phage lambda are compared. Strikingly similar 17 base pair units are found which we identify as the repressor binding sites. Each operator contains multiple repressor binding sites separated by A-T rich spacers. Elements of 2 fold rotational ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(75)90018-5
更新日期:1975-06-01 00:00:00
abstract::We report that the 12,000 dalton domain of fibronectin that interacts with fibroblast cell surfaces also binds specifically to thrombin-inducible, saturable receptors on platelets. Furthermore, we have used chemical cross-linking and monoclonal antibodies to show that the 12,000 dalton domain of fibronectin interacts ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90101-1
更新日期:1985-09-01 00:00:00
abstract::Vanadate has been shown to inhibit phosphatases that remove phosphate groups from phosphotyrosine in cell-free systems. Addition of vanadate to the culture medium of NRK-1 cells resulted in a maximal 40-fold increase in the level of phosphotyrosine in cell protein. Also, vanadate induced transformation as evidenced by...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(85)80051-9
更新日期:1985-07-01 00:00:00
abstract::Huntington's disease (HD) is an incurable neuropsychiatric disease associated with CAG repeat expansion within a widely expressed gene that causes selective neuronal death. To understand its normal function, we have created a targeted disruption in exon 5 of Hdh (Hdhex5), the murine homolog of the HD gene. Homozygotes...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90542-1
更新日期:1995-06-02 00:00:00
abstract::The sulfated glycosaminoglycan, heparin, was found to release 125I-labeled low density lipoprotein (125I-LDL) from its receptor site on the surface of normal human fibroblasts. Measurement of the amount of 125I-LDL released by heparin permitted the resolution of the total cellular uptake of 125I-LDL at 37 degrees C in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(76)90258-0
更新日期:1976-01-01 00:00:00
abstract::Wnt signaling plays critical roles in development of various organs and pathogenesis of many diseases, and augmented Wnt signaling has recently been implicated in mammalian aging and aging-related phenotypes. We here report that complement C1q activates canonical Wnt signaling and promotes aging-associated decline in ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.03.047
更新日期:2012-06-08 00:00:00
abstract::Loss of cell cycle control and acquisition of chromosomal rearrangements such as gene amplification often occur during tumor progression, suggesting that they may be correlated. We show here that the wild-type p53 allele is lost when fibroblasts from patients with the Li-Fraumeni syndrome (LFS) are passaged in vitro. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90244-7
更新日期:1992-09-18 00:00:00
abstract::The redox-regulated chaperone Hsp33 protects organisms against oxidative stress that leads to protein unfolding. Activation of Hsp33 is triggered by the oxidative unfolding of its own redox-sensor domain, making Hsp33 a member of a recently discovered class of chaperones that require partial unfolding for full chapero...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.01.045
更新日期:2012-03-02 00:00:00
abstract::T cells expressing chimeric antigen receptors (CARs) are promising cancer therapeutic agents, with the prospect of becoming the ultimate smart cancer therapeutics. To expand the capability of CAR T cells, here, we present a split, universal, and programmable (SUPRA) CAR system that simultaneously encompasses multiple ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.03.038
更新日期:2018-05-31 00:00:00
abstract::In this issue of Cell, Uderhardt et al. employed intravital two-photon microscopy to examine tissue-resident macrophage responses to sterile cellular injuries of variable size. They observed that while multi-cell "macrolesions" are characteristically pro-inflammatory, resident macrophages can "cloak" single-cell micro...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2019.03.042
更新日期:2019-04-18 00:00:00
abstract::Dysfunction and loss of insulin-producing pancreatic beta cells represent hallmarks of diabetes mellitus. Here, we show that mice lacking the mitogen-activated protein kinase (MAPK) p38delta display improved glucose tolerance due to enhanced insulin secretion from pancreatic beta cells. Deletion of p38delta results in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2008.11.018
更新日期:2009-01-23 00:00:00