Abstract:
:The initiation of chromosome segregation at anaphase is linked by the spindle assembly checkpoint to the completion of chromosome-microtubule attachment during metaphase. To determine the function of the mitotic checkpoint protein Mad2 during normal cell division and when mitosis goes awry, we have knocked out Mad2 in mice. We find that E5.5 embryonic cells lacking Mad2, like mad2 yeast, grow normally but are unable to arrest in response to spindle disruption. At E6.5, the cells of the epiblast begin rapid cell division and the absence of a checkpoint results in widespread chromosome missegregation and apoptosis. In contrast, the postmitotic trophoblast giant cells survive without Mad2. Thus, the spindle assembly checkpoint is required for accurate chromosome segregation in mitotic mouse cells, and for embryonic viability, even in the absence of spindle damage.
journal_name
Celljournal_title
Cellauthors
Dobles M,Liberal V,Scott ML,Benezra R,Sorger PKdoi
10.1016/s0092-8674(00)80875-2subject
Has Abstractpub_date
2000-06-09 00:00:00pages
635-45issue
6eissn
0092-8674issn
1097-4172pii
S0092-8674(00)80875-2journal_volume
101pub_type
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