Abstract:
:Bacterial gene expression depends not only on specific regulatory mechanisms, but also on bacterial growth, because important global parameters such as the abundance of RNA polymerases and ribosomes are all growth-rate dependent. Understanding of these global effects is necessary for a quantitative understanding of gene regulation and for the design of synthetic genetic circuits. We find that the observed growth-rate dependence of constitutive gene expression can be explained by a simple model using the measured growth-rate dependence of the relevant cellular parameters. More complex growth dependencies for genetic circuits involving activators, repressors, and feedback control were analyzed and verified experimentally with synthetic circuits. Additional results suggest a feedback mechanism mediated by general growth-dependent effects that does not require explicit gene regulation if the expressed protein affects cell growth. This mechanism can lead to growth bistability and promote the acquisition of important physiological functions such as antibiotic resistance and tolerance (persistence).
journal_name
Celljournal_title
Cellauthors
Klumpp S,Zhang Z,Hwa Tdoi
10.1016/j.cell.2009.12.001subject
Has Abstractpub_date
2009-12-24 00:00:00pages
1366-75issue
7eissn
0092-8674issn
1097-4172pii
S0092-8674(09)01505-0journal_volume
139pub_type
杂志文章相关文献
CELL文献大全abstract::Staphylococcal enterotoxin B is known to be a powerful T cell stimulant in mouse and man. In this paper we show that, for mice, this is because the protein in association with major histocompatibility complex class II molecules stimulates virtually all T cells bearing V beta 3 and V beta 8.1, 8.2, and 8.3, and few oth...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90980-x
更新日期:1989-01-13 00:00:00
abstract::Telomeres are specialized protein-DNA complexes that compose the ends of eukaryotic chromosomes. Telomeres protect chromosome termini from degradation and recombination and act together with telomerase to ensure complete genome replication. We have determined the crystal structure of the two-subunit Oxytricha nova tel...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81720-1
更新日期:1998-12-23 00:00:00
abstract::In developing organisms, spatially prescribed cell identities are thought to be determined by the expression levels of multiple genes. Quantitative tests of this idea, however, require a theoretical framework capable of exposing the rules and precision of cell specification over developmental time. We use the gap gene...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.01.007
更新日期:2019-02-07 00:00:00
abstract::V(D)J recombination of antigen receptor gene segments in B and T cells is mediated by the lymphoid-specific proteins RAG1 and RAG2. Now, Ji et al. (2010) demonstrate how RAG1 and RAG2 use DNA sequence specificity and modified histones within chromatin to target specific loci for V(D)J recombination at different stages...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2010.04.014
更新日期:2010-04-30 00:00:00
abstract::Cell lines from rhabdomyosarcomas, which are tumors of muscle origin, have been used as models of CD4-independent HIV infection. These cell lines can be induced to differentiate in vitro. We report here that the vpr gene of HIV1 is sufficient for the differentiation of the human rhabdomyosarcoma cell line TE671. Diffe...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90073-y
更新日期:1993-02-26 00:00:00
abstract::In order to identify nucleotide sequences required for efficient and accurate polyadenylation of mRNA precursors, we have constructed a series of mutations in the X. laevis beta 1-globin gene and analyzed transcripts produced upon microinjection into Xenopus oocytes. Small deletion and linker replacement mutations, wh...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90743-9
更新日期:1986-07-18 00:00:00
abstract::The nucleotide sequence from the 5' terminus inward of one third of mouse alpha- and beta maj-globin messenger RNAs has been established. In addition, using 5' 32P end-labeled mRNAs as substrates and S1 and T1 nucleases as probes for single-stranded regions, the secondary structures of mouse and rabbit alpha- and beta...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90391-8
更新日期:1980-01-01 00:00:00
abstract::S. cerevisiae inherits its vacuole by projecting vacuole-derived membrane vesicles and tubules into the bud, where they fuse to establish the daughter vacuole. This homotypic fusion event can be assayed in vitro. It requires Sec17p and Sec18p, the homologs of the mammalian alpha-SNAP and NSF, which cooperate in multip...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81084-3
更新日期:1996-04-05 00:00:00
abstract::Phagosomes were traditionally thought to originate from an invagination and scission of the plasma membrane to form a distinct intracellular vacuole. An alternative model implicating the endoplasmic reticulum (ER) as a major component of nascent and maturing phagosomes was recently proposed (Gagnon et al., 2002). To r...
journal_title:Cell
pub_type: 信件
doi:10.1016/j.cell.2005.08.018
更新日期:2005-10-07 00:00:00
abstract::The thymus exports a selected subset of virgin T lymphocytes to the peripheral lymphoid organs. The mature phenotype of these thymus emigrants is similar to that of medullary thymocytes and has been cited as supporting a medullary rather than cortical exit site. Using the monoclonal antibody MEL-14, we identify a 1%-3...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90529-4
更新日期:1984-08-01 00:00:00
abstract::The chronic autoimmune diseases include multiple complex genetic disorders. Recently, genome-wide association studies (GWAS) have identified a large number of major loci, with many associations shared between various autoimmune diseases. These associations highlight key roles for lymphocyte activation and prioritize s...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2010.03.003
更新日期:2010-03-19 00:00:00
abstract::We examined the interactions of lipid vesicles (liposomes) labeled with various fluorescent markers with the intracellular membranes of semi-intact ("perforated") fibroblasts. When incubations were performed in the presence of an ATP-regenerating system, both vesicle lipids and entrapped water soluble markers were tra...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(88)90135-3
更新日期:1988-12-02 00:00:00
abstract::Mammalian cells express a spectrum of tubulin isotypes whose relationship to the diversity of microtubule function is unknown. To examine whether different isotypes are segregated into functionally distinct microtubules, we generated immune sera capable of discriminating among the various naturally occurring beta-tubu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90456-9
更新日期:1987-05-22 00:00:00
abstract::Periodic transcription of yeast histone genes has been demonstrated by DNA excess filter hybridization of in vivo pulse-labeled RNA isolated from synchronous cell cultures. Using strains carrying cell division cycle (cdc) mutations, we show that both activation and termination of transcription are determined by tempor...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90036-8
更新日期:1982-08-01 00:00:00
abstract::Ríos et al. (2004) report in this issue that the Golgi protein GMAP-210 is sufficient to confer pericentrosomal positioning and recruits gamma-tubulin and associated microtubule-nucleating ring complex proteins to Golgi membranes. The results raise the possibility that short microtubules emanate from the Golgi to medi...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2004.07.015
更新日期:2004-08-06 00:00:00
abstract::We have isolated and characterized several members of the P transposable element family from a Drosophila melanogaster P strain. Large 2.9 kb elements are present as multiple highly conserved copies together with smaller (0.5-1.6 kb), heterogeneous elements. The complete DNA sequences of the 2.9 kb element and four sm...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90133-2
更新日期:1983-08-01 00:00:00
abstract::Many organisms expand the information content of their genome through enzymatic methylation of cytosine residues. Here we report the 2.8 A crystal structure of a bacterial DNA (cytosine-5)-methyltransferase (DCMtase), M. HaeIII, bound covalently to DNA. In this complex, the substrate cytosine is extruded from the DNA ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90060-8
更新日期:1995-07-14 00:00:00
abstract::To determine the special feature of ribosomal RNA promoters that might account for the highly efficient and regulated synthesis of rRNA in E. coli, we have analyzed the beginnings of two ribosomal RNA operons, rrnD and rrnX. DNA sequences for 425 bp preceding those specifying mature 16s rRNA are reported. In vitro tra...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90310-6
更新日期:1979-05-01 00:00:00
abstract::We have purified and characterized a modified peptide pheromone that accumulates in culture medium as B. subtilis grows to high density. This pheromone is required for the development of genetic competence. When added to cells at low density, the pheromone induces the premature development of competence. The peptide m...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90313-1
更新日期:1994-04-22 00:00:00
abstract::A novel trimolecular mechanism of microbial attachment to mammalian host cells was characterized for the obligate intracellular pathogen Chlamydia trachomatis. Using purified glycosaminoglycans (GAGs) and specific GAG lyases, we demonstrated that a heparan sulfate-like GAG present on the surface of chlamydia organisms...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90296-o
更新日期:1992-05-29 00:00:00
abstract::Two decades of research identified more than a dozen clock genes and defined a biochemical feedback mechanism of circadian oscillator function. To identify additional clock genes and modifiers, we conducted a genome-wide small interfering RNA screen in a human cellular clock model. Knockdown of nearly 1000 genes reduc...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.08.031
更新日期:2009-10-02 00:00:00
abstract::The structure of the complex formed by the arginine-rich motif of the transcriptional antitermination protein N of phage lambda and boxB RNA was determined by heteronuclear magnetic resonance spectroscopy. A bent alpha helix in N recognizes primarily the shape and negatively charged surface of the boxB hairpin through...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81579-2
更新日期:1998-04-17 00:00:00
abstract::The L7/12 stalk of the large subunit of bacterial ribosomes encompasses protein L10 and multiple copies of L7/12. We present crystal structures of Thermotoga maritima L10 in complex with three L7/12 N-terminal-domain dimers, refine the structure of an archaeal L10E N-terminal domain on the 50S subunit, and identify th...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2005.04.015
更新日期:2005-07-01 00:00:00
abstract::Independently derived mutants of Chinese hamster ovary cells have been isolated and shown to exhibit a subtle glycosylation defect resulting in the premature termination of certain asparagine-linked carbohydrate moieties. This carbohydrate alteration is akin to the types of structural variation termed microheterogenei...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90440-2
更新日期:1981-03-01 00:00:00
abstract::We have adopted Drosophila tissue culture cells as a host system for studying the structure and function of mammalian transcription factors. These cells provide an Sp1-deficient background and have been used in a complementation assay to identify functional domains of human transcription factor Sp1. The SV40 early pro...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(88)90144-4
更新日期:1988-12-02 00:00:00
abstract::The copy number of yeast histone genes was increased by inserting an extra H2A,H2B gene pair into the haploid genome by the technique of yeast transformation. The presence of this extra gene copy has no detectable effect on cell growth. The steady-state levels of histone H2A,H2B mRNAs are not elevated in transformed s...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90327-5
更新日期:1981-05-01 00:00:00
abstract::Eukaryotic Argonaute proteins strictly mediate RNA-guided RNA interference. In contrast, prokaryotic Argonautes can utilize DNA guides to target complementary DNA sequences to protect their hosts against invading DNA. In this issue of Cell, Jolly and colleagues demonstrate that Thermus thermophilus Argonaute additiona...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.08.037
更新日期:2020-09-17 00:00:00
abstract::The Ion gene of E. coli controls the stability of two bacteriophage lambda proteins. The functional half-life of the phage N gene product, measured by complementation, is increased about 5-fold in Ion mutant strains, from 2 min to 10 min. The chemical half-life of N protein, determined by its disappearance on polyacry...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90518-3
更新日期:1981-04-01 00:00:00
abstract::The endoplasmic reticulum (ER) often forms stacked membrane sheets, an arrangement that is likely required to accommodate a maximum of membrane-bound polysomes for secretory protein synthesis. How sheets are stacked is unknown. Here, we used improved staining and automated ultrathin sectioning electron microscopy meth...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.06.031
更新日期:2013-07-18 00:00:00
abstract::The pluripotent state of embryonic stem cells (ESCs) provides a unique perspective on regulatory programs that govern self-renewal and differentiation and somatic cell reprogramming. Here, we review the highly connected protein and transcriptional networks that maintain pluripotency and how they are intertwined with f...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2011.05.019
更新日期:2011-06-10 00:00:00