Abstract：
:Phagosomes were traditionally thought to originate from an invagination and scission of the plasma membrane to form a distinct intracellular vacuole. An alternative model implicating the endoplasmic reticulum (ER) as a major component of nascent and maturing phagosomes was recently proposed (Gagnon et al., 2002). To reconcile these seemingly disparate hypotheses, we used a combination of biochemical, fluorescence imaging, and electron microscopy techniques to quantitatively and dynamically assess the contribution of the plasmalemma and of the ER to phagosome formation and maturation. We could not verify even a transient physical continuity between the ER and the plasma membrane, nor were we able to detect a significant contribution of the ER to forming or maturing phagosomes in either macrophages or dendritic cells. Instead, our data indicate that the plasma membrane is the main constituent of nascent and newly formed phagosomes, which are progressively remodeled by fusion with endosomal and eventually lysosomal compartments as phagosomes mature into acidic, degradative organelles.
journal_name
Celljournal_title
Cellauthors
Touret N,Paroutis P,Terebiznik M,Harrison RE,Trombetta S,Pypaert M,Chow A,Jiang A,Shaw J,Yip C,Moore HP,van der Wel N,Houben D,Peters PJ,de Chastellier C,Mellman I,Grinstein Sdoi
10.1016/j.cell.2005.08.018subject
Has Abstractpub_date
2005-10-07 00:00:00pages
157-70issue
1eissn
0092-8674issn
1097-4172pii
S0092-8674(05)00822-6journal_volume
123pub_type
信件相关文献
CELL文献大全abstract：:The classical model for the activation of the nucleotide exchange factor Son of sevenless (SOS) involves its recruitment to the membrane, where it engages Ras. The recent discovery that Ras*GTP is an allosteric activator of SOS indicated that the regulation of SOS is more complex than originally envisaged. We now pres...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2004.10.005
更新日期：2004-10-29 00:00:00
abstract：:DNA double-strand breaks (DSBs) in B lymphocytes arise stochastically during replication or as a result of targeted DNA damage by activation-induced cytidine deaminase (AID). Here we identify recurrent, early replicating, and AID-independent DNA lesions, termed early replication fragile sites (ERFSs), by genome-wide l...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2013.01.006
更新日期：2013-01-31 00:00:00
abstract：:Mouse macrophages and lymphocytes express two distinct isoforms of a single class of Fc receptor for IgG. The macrophage isoform (FcRII-B2) is identical to the lymphocyte isoform (FcRII-B1) except for an inframe insertion in the cytoplasmic tail of FcRII-B1 that increases its length from 47 to 94 amino acids. To deter...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(89)90846-5
更新日期：1989-07-28 00:00:00
abstract：:Multisite hyperphosphorylation of tau has been implicated in the pathogenesis of neurodegenerative diseases including Alzheimer's disease (AD). However, the phosphorylation events critical for tau toxicity and mechanisms regulating these events are largely unknown. Here we show that Drosophila PAR-1 kinase initiates t...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(04)00170-9
更新日期：2004-03-05 00:00:00
abstract：:The human immunodeficiency virus, the lentivirus that causes AIDS, is responsible for the most prevalent epidemic in the history of mankind. Here in this Timeline, we have attempted to illustrate a short history of HIV-1, from its identification in landmark papers published by Robert Gallo, Myron Essex, and Luc Montag...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.09.004
更新日期：2020-10-15 00:00:00
abstract：:Immunofluorescent staining of a pericellular matrix produced by cultured human embryonic skin fibroblasts showed a codistribution among fibronectin, heparan sulfate proteoglycans and part of the chondroitin sulfate in a fibrillar network. Isolated matrix in an "intact" form could be scraped off the dish after detergen...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(82)90221-5
更新日期：1982-03-01 00:00:00
abstract：:Development of microbiota-directed foods (MDFs) that selectively increase the abundance of beneficial human gut microbes, and their expressed functions, requires knowledge of both the bioactive components of MDFs and the mechanisms underlying microbe-microbe interactions. Here, gnotobiotic mice were colonized with a d...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.08.011
更新日期：2019-09-19 00:00:00
abstract：:Vascular endothelial growth factor (VEGF), a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. We now describe the purification and the expression cloning from tumor cells of a third VEGF receptor, one that binds VEGF165 but not VEGF121. This isoform-specific VEGF receptor (...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81402-6
更新日期：1998-03-20 00:00:00
abstract：:Cholera toxin is assembled from two subunits in the periplasm of Vibrio cholerae and disassembled in the analogous compartment of target cells, the lumen of the endoplasmic reticulum (ER), before a fragment of it, the A1 chain, is transported into the cytosol. We show that protein disulfide isomerase (PDI) in the ER l...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(01)00289-6
更新日期：2001-03-23 00:00:00
abstract：:The switch from membrane-bound to secreted-form IgM that occurs during differentiation of B lymphocytes has long been known to involve regulated processing of the heavy chain pre-mRNA. Here, we show that accumulation of one subunit of an essential polyadenylation factor (CstF-64) is specifically repressed in mouse pri...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)82000-0
更新日期：1996-11-29 00:00:00
abstract：:In this issue, Stetson et al. (2008) report a mechanism by which host cells avert an autoimmune response to self-nucleic acids. They show that the nuclease Trex1 prevents the accumulation of DNA derived from endogenous retroelements that, if left unchecked, trigger elevated production of type I interferons leading to ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2008.08.010
更新日期：2008-08-22 00:00:00
abstract：:RECQL5 is the sole member of the RECQ family of helicases associated with RNA polymerase II (RNAPII). We now show that RECQL5 is a general elongation factor that is important for preserving genome stability during transcription. Depletion or overexpression of RECQL5 results in corresponding shifts in the genome-wide R...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.03.048
更新日期：2014-05-22 00:00:00
abstract：:An adaptive variant of the human Ectodysplasin receptor, EDARV370A, is one of the strongest candidates of recent positive selection from genome-wide scans. We have modeled EDAR370A in mice and characterized its phenotype and evolutionary origins in humans. Our computational analysis suggests the allele arose in centra...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.01.016
更新日期：2013-02-14 00:00:00
abstract：:Transcriptional silencing is a phenomenon in which the transcription of a gene by RNA polymerase II or III is repressed or not, dependent only on the gene's chromosomal location. Two prevailing models exist for silencing: (1) steric hindrance in silenced chromatin inhibits the binding of upstream activator proteins or...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2004.11.030
更新日期：2005-01-14 00:00:00
abstract：:Solute carrier (SLC) membrane transport proteins control essential physiological functions, including nutrient uptake, ion transport, and waste removal. SLCs interact with several important drugs, and a quarter of the more than 400 SLC genes are associated with human diseases. Yet, compared to other gene families of s...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2015.07.022
更新日期：2015-07-30 00:00:00
abstract：:An "off-the-shelf" cell therapy derived from induced pluripotent stem cells (iPSCs) has entered clinical trials in the United States. Other companies are following suit, harnessing iPSCs' self-renewal ability to manufacture cell therapies that don't require customization for each patient. But some experts aren't sure ...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2019.05.043
更新日期：2019-06-13 00:00:00
abstract：:Hybrids between mouse hepatoma cells (which secrete several serum proteins) and mouse or rat fibroblasts (which do not secrete these proteins) produce transferrin and the third component of complement (C3) like the parental hepatoma cells, while they do not secrete either albumin or alpha-fetoprotein (AFP). This lack ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(75)90073-2
更新日期：1975-09-01 00:00:00
abstract：:Designing successful microbiota-based therapies requires in-depth understanding of the ecological foundations of this community. In this issue, two studies by Whitaker et al. and Lim et al. provide refined genetic tools for dissecting the spatial organization and temporal dynamics of bacterial communities at the singl...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2017.04.009
更新日期：2017-04-20 00:00:00
abstract：:Ebola virus (EBOV) remains a public health threat. We performed a longitudinal study of B cell responses to EBOV in four survivors of the 2014 West African outbreak. Infection induced lasting EBOV-specific immunoglobulin G (IgG) antibodies, but their subclass composition changed over time, with IgG1 persisting, IgG3 r...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.04.036
更新日期：2019-05-30 00:00:00
abstract：:The peripheral nervous system has remarkable regenerative capacities in that it can repair a fully cut nerve. This requires Schwann cells to migrate collectively to guide regrowing axons across a 'bridge' of new tissue, which forms to reconnect a severed nerve. Here we show that blood vessels direct the migrating cord...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.07.021
更新日期：2015-08-27 00:00:00
abstract：:Many organisms expand the information content of their genome through enzymatic methylation of cytosine residues. Here we report the 2.8 A crystal structure of a bacterial DNA (cytosine-5)-methyltransferase (DCMtase), M. HaeIII, bound covalently to DNA. In this complex, the substrate cytosine is extruded from the DNA ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90060-8
更新日期：1995-07-14 00:00:00
abstract：:We have cloned and characterized the rat telomerase protein component 1 gene (TLP1), which is related to the gene for Tetrahymena p80. The cDNA encodes a 2629 amino acid sequence and produces the TLP1 proteins p240 and p230. The anti-TLP1 antibody specifically immunoprecipitated the telomerase activity. Moreover, p240...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81933-9
更新日期：1997-03-21 00:00:00
abstract：:A mechanism by which the nerve growth factor (NGF) signal is transduced to the nucleus to induce gene expression has been characterized. An NGF-inducible, Ras-dependent protein kinase has been identified that catalyzes the phosphorylation of the cyclic AMP response element-binding protein (CREB) at Ser-133. Phosphoryl...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90055-8
更新日期：1994-06-03 00:00:00
abstract：:We present genetic evidence that the tetracycline resistance element Tn10 transposes by a nonreplicative mechanism. Heteroduplex Tn10 elements containing three single base pair mismatches were constructed on lambda phage genomes and allowed to transpose from lambda into the bacterial chromosome. Analysis of TetR colon...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(86)90555-6
更新日期：1986-06-20 00:00:00
abstract：:We demonstrate that mutations in the human Ca(2+)-sensing receptor gene cause familial hypocalciuric hypercalcemia (FHH) and neonatal severe hyperparathyroidism (NSHPT), two inherited conditions characterized by altered calcium homeostasis. The Ca(2+)-sensing receptor belongs to the superfamily of seven membrane-spann...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(93)90617-y
更新日期：1993-12-31 00:00:00
abstract：:Fractionation of a microsomal detergent extract with ammonium sulfate allows separation of the signal recognition particle receptor (SR alpha), which is required for targeting of the nascent chain, from other microsomal proteins, such as signal peptidase, whose activity is displayed during subsequent translocation. Th...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90091-c
更新日期：1991-05-17 00:00:00
abstract：:Autophagy is a cell-autonomous, catabolic process that plays context-dependent roles in tumor growth and progression. Wei et al. report that EGFR signaling promotes tumor growth through phosphorylation and functional inactivation of Beclin 1 and the consequent suppression of autophagy. ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2013.08.040
更新日期：2013-09-12 00:00:00
abstract：:Neurons need to alter their response to a given stimulus over time in order for the animal to modify its behavior within a changing environment. Chen et al. now demonstrate that neuronal structure and function are altered coordinately by the history of the cell's activity through an unexpected molecular pathway. ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2012.09.008
更新日期：2012-09-28 00:00:00
abstract：:Antisense Piwi-interacting RNAs (piRNAs) guide silencing of established transposons during germline development, and sense piRNAs drive ping-pong amplification of the antisense pool, but how the germline responds to genome invasion is not understood. The KoRV-A gammaretrovirus infects the soma and germline and is swee...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.09.002
更新日期：2019-10-17 00:00:00
abstract：:The C-terminal domain (CTD) of the largest subunit of yeast RNA polymerase II contains 26-27 tandem copies of a conserved heptapeptide of unknown function. Yeast strains whose CTD contains ten heptamers are viable but defective for transcription of the INO1 gene and cold sensitive for growth. Deletion of the SIN1 gene...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90268-4
更新日期：1991-03-22 00:00:00
