Abstract:
:We present genetic evidence that the tetracycline resistance element Tn10 transposes by a nonreplicative mechanism. Heteroduplex Tn10 elements containing three single base pair mismatches were constructed on lambda phage genomes and allowed to transpose from lambda into the bacterial chromosome. Analysis of TetR colonies resulting from such transpositions suggests that information from both strands of the transposing Tn10 element is transmitted faithfully to its transposition product. The simplest interpretation of these results is that the transposing element is excised from the donor molecule and inserted into the target molecule without being replicated. A mismatch 70 base pairs from one end of the transposon is preserved, suggesting that there is little or no replication, even at the termini of the element, during transposition in vivo.
journal_name
Celljournal_title
Cellauthors
Bender J,Kleckner Ndoi
10.1016/0092-8674(86)90555-6subject
Has Abstractpub_date
1986-06-20 00:00:00pages
801-15issue
6eissn
0092-8674issn
1097-4172pii
0092-8674(86)90555-6journal_volume
45pub_type
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