Secondary structure of mouse and rabbit alpha- and beta-globin mRNAs: differential accessibility of alpha and beta initiator AUG codons towards nucleases.


:The nucleotide sequence from the 5' terminus inward of one third of mouse alpha- and beta maj-globin messenger RNAs has been established. In addition, using 5' 32P end-labeled mRNAs as substrates and S1 and T1 nucleases as probes for single-stranded regions, the secondary structures of mouse and rabbit alpha- and beta-globin mRNAs have been analyzed. Our results indicate that the AUG initiator codon in both mouse and rabbit beta-globin mRNA is quite susceptible to cleavage with S1 and T1 nucleases, suggesting that it resides in a single-stranded exposed region. In contrast, the initiator AUG in the alpha-globin mRNA of both species is inaccessible to cleavage, indicating that it is either buried by tertiary structure or is base-paired. Since the rate of initiation of protein synthesis with beta-globin mRNA in rabbit reticulocyte is 30--40% faster than for alpha-globin mRNA, these results imply a possible correlation between the differential rates of initiation with these two mRNAs and the accessibility of the respective AUG initiator codons.






Pavlakis GN,Lockard RE,Vamvakopoulos N,Rieser L,RajBhandary UL,Vournakis JN




Has Abstract


1980-01-01 00:00:00
















  • Single-stranded DNA transposition is coupled to host replication.

    abstract::DNA transposition has contributed significantly to evolution of eukaryotes and prokaryotes. Insertion sequences (ISs) are the simplest prokaryotic transposons and are divided into families on the basis of their organization and transposition mechanism. Here, we describe a link between transposition of IS608 and ISDra2...


    pub_type: 杂志文章


    authors: Ton-Hoang B,Pasternak C,Siguier P,Guynet C,Hickman AB,Dyda F,Sommer S,Chandler M

    更新日期:2010-08-06 00:00:00

  • The V beta-specific superantigen staphylococcal enterotoxin B: stimulation of mature T cells and clonal deletion in neonatal mice.

    abstract::Staphylococcal enterotoxin B is known to be a powerful T cell stimulant in mouse and man. In this paper we show that, for mice, this is because the protein in association with major histocompatibility complex class II molecules stimulates virtually all T cells bearing V beta 3 and V beta 8.1, 8.2, and 8.3, and few oth...


    pub_type: 杂志文章


    authors: White J,Herman A,Pullen AM,Kubo R,Kappler JW,Marrack P

    更新日期:1989-01-13 00:00:00

  • Isolation of two genes that affect mitotic chromosome transmission in S. cerevisiae.

    abstract::Two DNA sequences that reduce mitotic fidelity of chromosome transmission have been identified: MIF1 and MIF2. MIF1 is a unique sequence located on the right arm of chromosome XII that stimulates loss and recombination for both chromosomes V and VII when present in a high copy number plasmid. MIF1 is not essential for...


    pub_type: 杂志文章


    authors: Meeks-Wagner D,Wood JS,Garvik B,Hartwell LH

    更新日期:1986-01-17 00:00:00

  • Ulcerative colitis-like disease in mice with a disrupted interleukin-2 gene.

    abstract::Mice deficient for interleukin-2 develop normally during the first 3-4 weeks of age. However, later on they become severely compromised, and about 50% of the animals die between 4 and 9 weeks after birth. Of the remaining mice, 100% develop an inflammatory bowel disease with striking clinical and histological similari...


    pub_type: 杂志文章


    authors: Sadlack B,Merz H,Schorle H,Schimpl A,Feller AC,Horak I

    更新日期:1993-10-22 00:00:00

  • The in vitro transcription of the 7SK RNA gene by RNA polymerase III is dependent only on the presence of an upstream promoter.

    abstract::Deletion analysis was carried out on the human 7SK RNA gene to map regions essential for in vitro transcription by RNA polymerase III. The sequence promoting transcription is located between 37 and 3 bp upstream of the 7SK RNA coding region. RNA polymerase III transcription of adjacent plasmid sequences can be directe...


    pub_type: 杂志文章


    authors: Murphy S,Di Liegro C,Melli M

    更新日期:1987-10-09 00:00:00

  • Crystal structure of the A domain from the alpha subunit of integrin CR3 (CD11b/CD18).

    abstract::We have determined the high resolution crystal structure of the A domain from the alpha chain of integrin CR3. The domain adopts a classic alpha/beta "Rossmann" fold and contains an unusual Mg2+ coordination site at its surface. One of the coordinating ligands is the glutamate side chain from another A domain molecule...


    pub_type: 杂志文章


    authors: Lee JO,Rieu P,Arnaout MA,Liddington R

    更新日期:1995-02-24 00:00:00

  • Case Study: Prolonged Infectious SARS-CoV-2 Shedding from an Asymptomatic Immunocompromised Individual with Cancer.

    abstract::Long-term severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) shedding was observed from the upper respiratory tract of a female immunocompromised individual with chronic lymphocytic leukemia and acquired hypogammaglobulinemia. Shedding of infectious SARS-CoV-2 was observed up to 70 days, and of genomic and s...


    pub_type: 杂志文章


    authors: Avanzato VA,Matson MJ,Seifert SN,Pryce R,Williamson BN,Anzick SL,Barbian K,Judson SD,Fischer ER,Martens C,Bowden TA,de Wit E,Riedo FX,Munster VJ

    更新日期:2020-12-23 00:00:00

  • Genes that act before conjugation to prepare the Saccharomyces cerevisiae nucleus for caryogamy.

    abstract::Mutations in four nuclear genes, kar1 cdc4, 28, and 37, block or impair nuclear fusion during conjugation of Saccharomyces cerevisiae. Mutations in all four genes are recessive for the caryogamy defect; in matings between diploid cells both of which are heterozygous for any one of the four mutations (-/+ X -/+), caryo...


    pub_type: 杂志文章


    authors: Dutcher SK,Hartwell LH

    更新日期:1983-05-01 00:00:00

  • Mobility of histones on the chromosome of simian virus 40.

    abstract::Linear simian virus 40 (SV40) chromosomes were prepared by Eco R1 nuclease cleavage of the circular SV40 chromosomes released from virions with dithiothreitol at pH 9,8. Chromatin-DNA hybrids were constructed with segments of 3H-labeled, naked SV40 DNA covalently joined via the Eco R1-generated cohesive ends to segmen...


    pub_type: 杂志文章


    authors: Beard P

    更新日期:1978-11-01 00:00:00

  • The membrane-spanning segment of invariant chain (I gamma) contains a potentially cleavable signal sequence.

    abstract::The human invariant chain (I gamma) of class II histocompatibility antigens spans the membrane of the endoplasmic reticulum once. It exposes a small amino-terminal domain on the cytoplasmic side and a carboxy-terminal, glycosylated domain on the exoplasmic side of the membrane. When the exoplasmic domain of I gamma is...


    pub_type: 杂志文章


    authors: Lipp J,Dobberstein B

    更新日期:1986-09-26 00:00:00

  • tudor, a gene required for assembly of the germ plasm in Drosophila melanogaster.

    abstract::Developmental analysis of a newly isolated maternal effect grandchildless mutant, tudor (tud), in Drosophila melanogaster indicates that tud+ activity is required during oogenesis for the determination and/or formation of primordial germ cells (pole cells) and for normal embryonic abdominal segmentation. Regardless of...


    pub_type: 杂志文章


    authors: Boswell RE,Mahowald AP

    更新日期:1985-11-01 00:00:00

  • S. cerevisiae chromosomes biorient via gradual resolution of syntely between S phase and anaphase.

    abstract::Following DNA replication, eukaryotic cells must biorient all sister chromatids prior to cohesion cleavage at anaphase. In animal cells, sister chromatids gradually biorient during prometaphase, but current models of mitosis in S. cerevisiae assume that biorientation is established shortly after S phase. This assumpti...


    pub_type: 杂志文章


    authors: Marco E,Dorn JF,Hsu PH,Jaqaman K,Sorger PK,Danuser G

    更新日期:2013-08-29 00:00:00

  • Tumor promoter-induced changes in the permeability of epithelial cell tight junctions.

    abstract::Treatment of the kidney epithelial cell line MDCK with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) induces a large increase in the permeability of tight junctions within 2-4 hr. This change in transepithelial permeability is accompanied by alterations in cell morphology that include the opening of ti...


    pub_type: 杂志文章


    authors: Ojakian GK

    更新日期:1981-01-01 00:00:00

  • Organoid cultures derived from patients with advanced prostate cancer.

    abstract::The lack of in vitro prostate cancer models that recapitulate the diversity of human prostate cancer has hampered progress in understanding disease pathogenesis and therapy response. Using a 3D organoid system, we report success in long-term culture of prostate cancer from biopsy specimens and circulating tumor cells....


    pub_type: 杂志文章


    authors: Gao D,Vela I,Sboner A,Iaquinta PJ,Karthaus WR,Gopalan A,Dowling C,Wanjala JN,Undvall EA,Arora VK,Wongvipat J,Kossai M,Ramazanoglu S,Barboza LP,Di W,Cao Z,Zhang QF,Sirota I,Ran L,MacDonald TY,Beltran H,Mosquera J

    更新日期:2014-09-25 00:00:00

  • Adaptive immunity to SARS-CoV-2 and COVID-19.

    abstract::The adaptive immune system is important for control of most viral infections. The three fundamental components of the adaptive immune system are B cells (the source of antibodies), CD4+ T cells, and CD8+ T cells. The armamentarium of B cells, CD4+ T cells, and CD8+ T cells has differing roles in different viral infect...


    pub_type: 杂志文章,评审


    authors: Sette A,Crotty S

    更新日期:2021-01-12 00:00:00

  • The complete sequence of a chromosomal mouse alpha--globin gene reveals elements conserved throughout vertebrate evolution.

    abstract::The mammalian alpha- and beta--globin genes are thought to have evolved from a common ancestral sequence by a duplication event that occurred over 500 million years ago. We have now determined the entire nucleotide sequence of a cloned mouse alpha--globin gene, including regions that flank and interrupt the coding seq...


    pub_type: 杂志文章


    authors: Nishioka Y,Leder P

    更新日期:1979-11-01 00:00:00

  • Bcl2 regulation by the melanocyte master regulator Mitf modulates lineage survival and melanoma cell viability.

    abstract::Kit/SCF signaling and Mitf-dependent transcription are both essential for melanocyte development and pigmentation. To identify Mitf-dependent Kit transcriptional targets in primary melanocytes, microarray studies were undertaken. Among identified targets was BCL2, whose germline deletion produces melanocyte loss and w...


    pub_type: 杂志文章


    authors: McGill GG,Horstmann M,Widlund HR,Du J,Motyckova G,Nishimura EK,Lin YL,Ramaswamy S,Avery W,Ding HF,Jordan SA,Jackson IJ,Korsmeyer SJ,Golub TR,Fisher DE

    更新日期:2002-06-14 00:00:00

  • Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery.

    abstract::Growth factors can promote cell survival by activating the phosphatidylinositide-3'-OH kinase and its downstream target, the serine-threonine kinase Akt. However, the mechanism by which Akt functions to promote survival is not understood. We show that growth factor activation of the PI3'K/Akt signaling pathway culmina...


    pub_type: 杂志文章


    authors: Datta SR,Dudek H,Tao X,Masters S,Fu H,Gotoh Y,Greenberg ME

    更新日期:1997-10-17 00:00:00

  • Phase transition of spindle-associated protein regulate spindle apparatus assembly.

    abstract::Spindle assembly required during mitosis depends on microtubule polymerization. We demonstrate that the evolutionarily conserved low-complexity protein, BuGZ, undergoes phase transition or coacervation to promote assembly of both spindles and their associated components. BuGZ forms temperature-dependent liquid droplet...


    pub_type: 杂志文章


    authors: Jiang H,Wang S,Huang Y,He X,Cui H,Zhu X,Zheng Y

    更新日期:2015-09-24 00:00:00

  • The Arabidopsis GNOM ARF-GEF mediates endosomal recycling, auxin transport, and auxin-dependent plant growth.

    abstract::Exchange factors for ARF GTPases (ARF-GEFs) regulate vesicle trafficking in a variety of organisms. The Arabidopsis protein GNOM is a brefeldin A (BFA) sensitive ARF-GEF that is required for the proper polar localization of PIN1, a candidate transporter of the plant hormone auxin. Mutations in GNOM lead to development...


    pub_type: 杂志文章


    authors: Geldner N,Anders N,Wolters H,Keicher J,Kornberger W,Muller P,Delbarre A,Ueda T,Nakano A,Jürgens G

    更新日期:2003-01-24 00:00:00

  • Preclinical mouse cancer models: a maze of opportunities and challenges.

    abstract::Significant advances have been made in developing novel therapeutics for cancer treatment, and targeted therapies have revolutionized the treatment of some cancers. Despite the promise, only about five percent of new cancer drugs are approved, and most fail due to lack of efficacy. The indication is that current precl...


    pub_type: 杂志文章,评审


    authors: Day CP,Merlino G,Van Dyke T

    更新日期:2015-09-24 00:00:00

  • Ligand and cation binding are dual functions of a discrete segment of the integrin beta 3 subunit: cation displacement is involved in ligand binding.

    abstract::The alpha IIb beta 3 integrin binds Arg-Gly-Asp-containing (RGD-containing) ligands in a cation-dependent interaction. A fourteen amino acid sequence, beta 3 (118-131), and an antibody to it, inhibited ligand binding functions of alpha IIb beta 3, and a 1:1 stoichiometric beta 3 (118-131)-RGD complex was detected by m...


    pub_type: 杂志文章


    authors: D'Souza SE,Haas TA,Piotrowicz RS,Byers-Ward V,McGrath DE,Soule HR,Cierniewski C,Plow EF,Smith JW

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  • Disease-Causing Mutations in the G Protein Gαs Subvert the Roles of GDP and GTP.

    abstract::The single most frequent cancer-causing mutation across all heterotrimeric G proteins is R201C in Gαs. The current model explaining the gain-of-function activity of the R201 mutations is through the loss of GTPase activity and resulting inability to switch off to the GDP state. Here, we find that the R201C mutation ca...


    pub_type: 杂志文章


    authors: Hu Q,Shokat KM

    更新日期:2018-05-17 00:00:00

  • Inducible NOS inhibition reverses tobacco-smoke-induced emphysema and pulmonary hypertension in mice.

    abstract::Chronic obstructive pulmonary disease (COPD) is one of the most common causes of death worldwide. We report in an emphysema model of mice chronically exposed to tobacco smoke that pulmonary vascular dysfunction, vascular remodeling, and pulmonary hypertension (PH) precede development of alveolar destruction. We provid...


    pub_type: 杂志文章


    authors: Seimetz M,Parajuli N,Pichl A,Veit F,Kwapiszewska G,Weisel FC,Milger K,Egemnazarov B,Turowska A,Fuchs B,Nikam S,Roth M,Sydykov A,Medebach T,Klepetko W,Jaksch P,Dumitrascu R,Garn H,Voswinckel R,Kostin S,Seeger W,S

    更新日期:2011-10-14 00:00:00

  • Neuron-Astrocyte Metabolic Coupling Protects against Activity-Induced Fatty Acid Toxicity.

    abstract::Metabolic coordination between neurons and astrocytes is critical for the health of the brain. However, neuron-astrocyte coupling of lipid metabolism, particularly in response to neural activity, remains largely uncharacterized. Here, we demonstrate that toxic fatty acids (FAs) produced in hyperactive neurons are tran...


    pub_type: 杂志文章


    authors: Ioannou MS,Jackson J,Sheu SH,Chang CL,Weigel AV,Liu H,Pasolli HA,Xu CS,Pang S,Matthies D,Hess HF,Lippincott-Schwartz J,Liu Z

    更新日期:2019-05-30 00:00:00

  • SnapShot: CRISPR-RNA-guided adaptive immune systems.

    abstract::Bacteria and archaea have evolved sophisticated adaptive immune systems that reply on CRISPR loci and a diverse cassette of Cas genes that are classified into three main types and at least eleven subtypes. All CRISPR-Cas immune systems operate through three main stages: acquisition, biogenesis, and interference. This ...


    pub_type: 杂志文章


    authors: Carter J,Wiedenheft B

    更新日期:2015-09-24 00:00:00

  • A human interactome in three quantitative dimensions organized by stoichiometries and abundances.

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    pub_type: 杂志文章


    authors: Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

    更新日期:2015-10-22 00:00:00

  • Switch-of-function mutants based on morphology classification of Ras superfamily small GTPases.

    abstract::Signaling proteins from the same family can have markedly different roles in a given cellular context. Here, we show that expression of one hundred constitutively active human small GTPases induced cell morphologies that fell into nine distinct classes. We developed an algorithm for pairs of classes that predicted ami...


    pub_type: 杂志文章


    authors: Heo WD,Meyer T

    更新日期:2003-05-02 00:00:00

  • A chromosome RNAissance.

    abstract::In RNA-mediated interference (RNAi), double-stranded RNAs (dsRNAs) target complementary mRNAs for degradation. New work demonstrates that essential chromosomal functions are mediated through RNAi protein components and short RNAs, which alter chromosome function at specific DNA loci via histone modification. ...


    pub_type: 杂志文章


    authors: Dernburg AF,Karpen GH

    更新日期:2002-10-18 00:00:00

  • Interspecies sex and taste.

    abstract::Different species of fruit flies share habitats but are believed to mate with each other only rarely. In this issue, Fan et al. show that interspecies mating is inhibited by the taste receptor Gr32a (Gustatory receptor 32a) and a neural circuit in which it functions. ...


    pub_type: 评论,杂志文章


    authors: Koh TW,Carlson JR

    更新日期:2013-07-03 00:00:00