Abstract:
:Ligand-directed signal bias offers opportunities for sculpting molecular events, with the promise of better, safer therapeutics. Critical to the exploitation of signal bias is an understanding of the molecular events coupling ligand binding to intracellular signaling. Activation of class B G protein-coupled receptors is driven by interaction of the peptide N terminus with the receptor core. To understand how this drives signaling, we have used advanced analytical methods that enable separation of effects on pathway-specific signaling from those that modify agonist affinity and mapped the functional consequence of receptor modification onto three-dimensional models of a receptor-ligand complex. This yields molecular insights into the initiation of receptor activation and the mechanistic basis for biased agonism. Our data reveal that peptide agonists can engage different elements of the receptor extracellular face to achieve effector coupling and biased signaling providing a foundation for rational design of biased agonists.
journal_name
Celljournal_title
Cellauthors
Wootten D,Reynolds CA,Smith KJ,Mobarec JC,Koole C,Savage EE,Pabreja K,Simms J,Sridhar R,Furness SGB,Liu M,Thompson PE,Miller LJ,Christopoulos A,Sexton PMdoi
10.1016/j.cell.2016.05.023subject
Has Abstractpub_date
2016-06-16 00:00:00pages
1632-1643issue
7eissn
0092-8674issn
1097-4172pii
S0092-8674(16)30571-2journal_volume
165pub_type
杂志文章相关文献
CELL文献大全abstract::Egg laying by the nematode Caenorhabditis elegans requires the functioning of the vulva, the gonad, the egg-laying muscles, and the two HSN neurons, which innervate these muscles. By analyzing a newly isolated mutant (dig-1) that displaces the gonad, we discovered that cell interactions coordinate the spatial relation...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90382-o
更新日期:1990-09-21 00:00:00
abstract::Chromosome segregation at meiosis I depends on pairing and crossing-over between homologs. In most eukaryotes, pairing culminates with formation of the proteinaceous synaptonemal complex (SC). In budding yeast, recombination initiates through double-strand DNA breaks (DSBs) and is thought to be essential for SC format...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81481-6
更新日期:1998-08-07 00:00:00
abstract::Notch signaling is broadly used to regulate cell-fate decisions. We have identified a gene, rumi, with a temperature-sensitive Notch phenotype. At 28 degrees C-30 degrees C, rumi clones exhibit a full-blown loss of Notch signaling in all tissues tested. However, at 18 degrees C only a mild Notch phenotype is evident. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.12.016
更新日期:2008-01-25 00:00:00
abstract::We examine the interaction between polyoma-virus-encoded middle tumor antigen and the cellular src gene product, pp60c-src, using a series of monoclonal antibodies that recognize mammalian pp60c-src. Our results show that infection of mouse cells with transformation-competent strains of polyoma virus results in the st...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90272-1
更新日期:1984-10-01 00:00:00
abstract::The microtubule-based metaphase spindle is subjected to forces that act in diverse orientations and over a wide range of timescales. Currently, we cannot explain how this dynamic structure generates and responds to forces while maintaining overall stability, as we have a poor understanding of its micromechanical prope...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.05.038
更新日期:2011-06-24 00:00:00
abstract::Pulsed field gradient gel electrophoresis fractionates chromosome-sized DNA molecules from T. brucei. About 60% of the DNA remains in or close to the gel slot (large DNA). There are about three chromosomes of approximately 2 Mb, at least six chromosomes of 200-700 kb, and roughly a hundred mini-chromosomes of 50-150 k...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90302-7
更新日期:1984-05-01 00:00:00
abstract::A novel method of affinity chromatography on insolubilized collagen-binding fragments of fibronectin was utilized to isolate a random-coil collagenous protein from culture media of mouse teratocarcinoma-derived endodermal cells. These cells also produced another collagenous protein, which did not bind to fibronectin b...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90164-7
更新日期:1982-06-01 00:00:00
abstract::Transfer of human chromosome 11, which contains the myoD locus, from primary fibroblasts into 10T1/2 cells results in activation of myoD. In contrast, hybrids that retain human chromosome 11 and additional human chromosomes fail to activate myoD. We show that human chromosome 4 inhibits myoD activation. myoD enhancer/...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90033-0
更新日期:1995-08-25 00:00:00
abstract::Liddle's syndrome is an inherited form of hypertension caused by mutations that truncate the C-terminus of human epithelial Na+ channel (hENaC) subunits. Expression of truncated beta and gamma hENaC subunits increased Na+ current. However, truncation did not alter single-channel conductance or open state probability, ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90212-0
更新日期:1995-12-15 00:00:00
abstract::Every day we make decisions critical for adaptation and survival. We repeat actions with known consequences. But we also draw on loosely related events to infer and imagine the outcome of entirely novel choices. These inferential decisions are thought to engage a number of brain regions; however, the underlying neuron...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.08.035
更新日期:2020-10-01 00:00:00
abstract::Enteric-associated neurons (EANs) are closely associated with immune cells and continuously monitor and modulate homeostatic intestinal functions, including motility and nutrient sensing. Bidirectional interactions between neuronal and immune cells are altered during disease processes such as neurodegeneration or irri...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.12.002
更新日期:2020-01-09 00:00:00
abstract::Antigen presenting cells (macrophages and dendritic cells) express pattern recognition molecules that are thought to recognize foreign ligands during early phases of the immune response. The best known of these are probably the Toll-like receptors, but a number of other receptors are also involved. Several of these re...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/s0092-8674(02)01201-1
更新日期:2002-12-27 00:00:00
abstract::Trained innate immunity fosters a sustained favorable response of myeloid cells to a secondary challenge, despite their short lifespan in circulation. We thus hypothesized that trained immunity acts via modulation of hematopoietic stem and progenitor cells (HSPCs). Administration of β-glucan (prototypical trained-immu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.11.034
更新日期:2018-01-11 00:00:00
abstract::The S. pombe nuc2+ gene is required for mitotic chromosome disjunction. Its mutation arrests mitosis at the metaphase. The gene product is present in the nuclear scaffold-like fraction. The nuc2+ protein contains a domain, separated from ten 34 amino acid repeat segments, that is capable of binding AT-rich DNA in vitr...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90746-2
更新日期:1990-01-26 00:00:00
abstract::The tra-1 gene plays a central role in C. elegans sex determination. XX animals develop as hermaphrodites when tra-1 is active and as males when it is not. By analyzing a large number of tra-1 genetic mosaics we have shown that, with the expected exception of vulval induction by the hermaphrodite gonad, tra-1 function...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90415-b
更新日期:1990-12-21 00:00:00
abstract::The nucleotide sequences of the 3' termini of the DNA from four autonomous rodent parvoviruses have been determined. The terminus of each genome exists as a Y-shaped hairpin structure involving 115 or 116 nucleotides. The sequence of this region of DNA is highly conserved and shows no evidence of internal sequence het...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90276-9
更新日期:1979-07-01 00:00:00
abstract::A recent series of papers, including Festa et al. (2011) in this issue, has revealed unexpected interdependent relationships among cell populations residing in and around the hair follicle. These interactions between different lineages of stem cells are crucial for hair follicle growth and cycling and point to a compl...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2011.08.020
更新日期:2011-09-02 00:00:00
abstract::A gene for Saccharomyces cerevisiae tRNATrp has been sequenced which contains an intervening sequence of 34 bp (H. S. Kang and J. Abelson, unpublished results). The mutant yeast strain ts-136 accumulates a precursor to tRNATrp which contains mature ends and is colinear with the tRNATrp gene. A nuclear extract from Xen...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90166-1
更新日期:1979-06-01 00:00:00
abstract::Recombinant DNA techniques have been used to isolate the integrated viral DNA, together with flanking cellular sequences, from three independently isolated lines of SV40-transformed BALB/c 3T3 cells. We have determined the structures of these cloned segments and their relationships to one another by restriction enzyme...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90073-8
更新日期:1981-08-01 00:00:00
abstract::Clinical benefits of cytokine blockade in ileal Crohn's disease (iCD) are limited to a subset of patients. Here, we applied single-cell technologies to iCD lesions to address whether cellular heterogeneity contributes to treatment resistance. We found that a subset of patients expressed a unique cellular module in inf...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.08.008
更新日期:2019-09-05 00:00:00
abstract::Voltage-gated calcium channels play a central role in regulating the electrical and biochemical properties of neurons and muscle cells. One of the ways in which calcium channels regulate long-lasting neuronal properties is by activating signaling pathways that control gene expression, but the mechanisms that link calc...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.10.017
更新日期:2006-11-03 00:00:00
abstract::Null mutant mice for retinoic acid receptor gamma 2 (RAR gamma 2) or all RAR gamma isoforms were generated. RAR gamma 2 mutants appeared normal, whereas RAR gamma mutants exhibited growth deficiency, early lethality, and male sterility due to squamous metaplasia of the seminal vesicles and prostate. These defects were...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90246-m
更新日期:1993-05-21 00:00:00
abstract::Angiogenesis is an important event for embryonic organogenesis as well as for tissue repair in the adult. Here, we show that hematopoietic stem cells (HSCs) play important roles for angiogenesis during embryogenesis. To investigate the role of HSCs in endothelial cell (EC) development, we analyzed AML1-deficient embry...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)00025-8
更新日期:2000-07-21 00:00:00
abstract::To defend cells against viruses, the MAVS (mitochondrial antiviral signaling) adaptor protein initiates an antiviral signaling cascade from mitochondrial membranes. In this issue, Dixit et al. (2010) show that MAVS also localizes to the membranes of peroxisomes, where it rapidly induces expression of a subset of antiv...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2010.04.043
更新日期:2010-05-14 00:00:00
abstract::The kinetics of association of epidermal growth factor (EGF) and diferric-transferrin (TF) with clathrin-coated membranes of KB cells were examined using anti-clathrin antibody bound to Staphylococcus aureus. The ligands were bound to cells at 4 degrees C and after warming to 30 degrees C for 5 min, both ligands were ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90006-0
更新日期:1984-12-01 00:00:00
abstract::Kinetochores direct attachment of chromosomes to microtubules of the mitotic spindle during cell division. Three recent studies in Cell, including one in this issue, reveal important new roles for two kinetochore protein complexes-Ndc80 and INCENP-Survivin-in establishing the correct attachment of chromosomes to spind...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2006.11.032
更新日期:2006-12-15 00:00:00
abstract::SR proteins constitute a family of pre-mRNA splicing factors that play important roles in both constitutive and regulated splicing. Here, we describe one member of the family, which we call SRp38, with unexpected properties. Unlike other SR proteins, SRp38 cannot activate splicing and is essentially inactive in splici...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(02)01038-3
更新日期:2002-11-01 00:00:00
abstract::This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal). This article has been retracted at the request of request of the editors. Cell is retracting this paper reporting structures of a poxvirus protein, VCP, that inhi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00214-8
更新日期:2001-01-26 00:00:00
abstract::We explored how the odor map in the Drosophila antennal lobe is represented in higher olfactory centers, the mushroom body and lateral horn. Systematic single-cell tracing of projection neurons (PNs) that send dendrites to specific glomeruli in the antennal lobe revealed their stereotypical axon branching patterns and...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(02)00700-6
更新日期:2002-04-19 00:00:00
abstract::We have analyzed the generation of unexpected patterns of RNA editing, i.e., those not following a strict 3' to 5' progression, which occur in junction regions between fully edited and preedited sequences. Evidence is presented that these patterns are generated by misediting due to specific events of misguiding. Mised...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90171-8
更新日期:1992-08-07 00:00:00