Abstract：
:The amino acid glutamine has a central role in nitrogen metabolism. Although the molecular mechanisms responsible for its transport across cell membranes remain poorly understood, classical amino acid transport system N appears particularly important. Using intracellular pH measurements, we have now identified an orphan protein related to a vesicular neurotransmitter transporter as system N. Functional analysis shows that this protein (SN1) involves H+ exchange as well as Na+ cotransport and, under physiological conditions, mediates glutamine efflux as well as uptake. Together with the pattern of SN1 expression, these unusual properties suggest novel physiological roles for system N in nitrogen metabolism and synaptic transmission.
journal_name
Celljournal_title
Cellauthors
Chaudhry FA,Reimer RJ,Krizaj D,Barber D,Storm-Mathisen J,Copenhagen DR,Edwards RHdoi
10.1016/s0092-8674(00)81674-8subject
Has Abstractpub_date
1999-12-23 00:00:00pages
769-80issue
7eissn
0092-8674issn
1097-4172pii
S0092-8674(00)81674-8journal_volume
99pub_type
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