Abstract:
:The hsp 70 gene of Drosophila contains three domains to which a heat shock gene specific transcription factor (HSTF) binds. In addition to the previously described 55 bp binding domain proximal to the TAT homology, there are two 25 bp binding sites farther upstream. Footprinting studies with 5' and 3' deletion mutations show two contiguous HSTF binding sites of different intrinsic affinities within the 55 bp binding domain. Determinations made with an agarose-acrylamide gel assay suggest that the HSTF possesses a 12.5-fold higher intrinsic affinity for the site closest to the TATA homology than for the more distal site. Binding of HSTF to the distal site thus appears cooperative, requiring occupancy of the first site. Transcription studies in vitro on the 5' deletions with nuclear extracts and reconstitution experiments show that the TATA proximal site alone, is insufficient for maximal transcriptional activation of the hsp 70 gene.
journal_name
Celljournal_title
Cellauthors
Topol J,Ruden DM,Parker CSdoi
10.1016/0092-8674(85)90110-2subject
Has Abstractpub_date
1985-09-01 00:00:00pages
527-37issue
2eissn
0092-8674issn
1097-4172pii
0092-8674(85)90110-2journal_volume
42pub_type
杂志文章相关文献
CELL文献大全abstract::During fruiting body development, the product of the csgA gene is necessary for cellular aggregation, for spore differentiation, and for gene expression that is initiated after 6 hr of starvation. From nascent wild-type fruiting bodies we have purified a polypeptide of 17 kd called C-factor, which, at approximately 1 ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90211-v
更新日期:1990-04-06 00:00:00
abstract::Complex gene regulatory networks require transcription factors (TFs) to bind distinct DNA sequences. To understand how novel TF specificity evolves, we combined phylogenetic, biochemical, and biophysical approaches to interrogate how DNA recognition diversified in the steroid hormone receptor (SR) family. After duplic...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.09.003
更新日期:2014-09-25 00:00:00
abstract::The gut epithelium self-renews every several days, providing an important innate defense system that limits bacterial colonization. Nevertheless, many bacterial pathogens, including Shigella, efficiently colonize the intestinal epithelium. Here, we show that the Shigella effector IpaB, when delivered into epithelial c...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.06.043
更新日期:2007-08-24 00:00:00
abstract::The chaperonin GroEL binds nonnative proteins too large to fit inside the productive GroEL-GroES cis cavity, but whether and how it assists their folding has remained unanswered. We have examined yeast mitochondrial aconitase, an 82 kDa monomeric Fe(4)S(4) cluster-containing enzyme, observed to aggregate in chaperonin...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00523-2
更新日期:2001-10-19 00:00:00
abstract::The mechanism by which cells decide to skip mitosis to become polyploid is largely undefined. Here we used a high-content image-based screen to identify small-molecule probes that induce polyploidization of megakaryocytic leukemia cells and serve as perturbagens to help understand this process. Our study implicates fi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.06.032
更新日期:2012-08-03 00:00:00
abstract::We have shown that in fixed mitotic chromosomes from female G. gerbillus cells the inactive X chromosome is distinctly less sensitive to DNAase I than the active X chromosome, as demonstrated by in situ nick translation. These results indicated that the specific chromatin conformation that renders potentially active g...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90504-x
更新日期:1984-09-01 00:00:00
abstract::Two-pore domain (K2P) K(+) channels are major regulators of excitability that endow cells with an outwardly rectifying background "leak" conductance. In some K2P channels, strong voltage-dependent activation has been observed, but the mechanism remains unresolved because they lack a canonical voltage-sensing domain. H...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.02.002
更新日期:2016-02-25 00:00:00
abstract::In eukaryotic cells, organelles and the cytoskeleton undergo highly dynamic yet organized interactions capable of orchestrating complex cellular functions. Visualizing these interactions requires noninvasive, long-duration imaging of the intracellular environment at high spatiotemporal resolution and low background. T...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.09.057
更新日期:2018-11-15 00:00:00
abstract::Nuclear matrices from undifferentiated and differentiated Friend erythroleukemia cells have been obtained by a method which removes DNA in a physiological buffer. These matrices preserved the characteristic topographical distribution of condensed and diffuse "chromatin" regions, as do nuclei in situ or isolated nuclei...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90221-6
更新日期:1979-12-01 00:00:00
abstract::VopL is an effector protein from Vibrio parahaemolyticus that nucleates actin filaments. VopL consists of a VopL C-terminal domain (VCD) and an array of three WASP homology 2 (WH2) motifs. Here, we report the crystal structure of the VCD dimer bound to actin. The VCD organizes three actin monomers in a spatial arrange...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.09.019
更新日期:2013-10-10 00:00:00
abstract::Mutually exclusive expression within the var gene family of the malaria parasite Plasmodium falciparum is important for parasite survival and virulence. In this issue of Cell, Duraisingh et al. and Freitas-Junior et al. provide evidence for the role of Sir2-dependent alterations in chromatin structure and changes in s...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2005.03.019
更新日期:2005-04-08 00:00:00
abstract::Constitutive cell-autonomous immunity in metazoans predates interferon-inducible immunity and comprises primordial innate defense. Phagocytes mobilize interferon-inducible responses upon engagement of well-characterized signaling pathways by pathogen-associated molecular patterns (PAMPs). The signals controlling deplo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.09.034
更新日期:2017-11-02 00:00:00
abstract::Cancer cell of origin is difficult to identify by analyzing cells within terminal stage tumors, whose identity could be concealed by the acquired plasticity. Thus, an ideal approach to identify the cell of origin is to analyze proliferative abnormalities in distinct lineages prior to malignancy. Here, we use mosaic an...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.06.014
更新日期:2011-07-22 00:00:00
abstract::DNA repair was measured in the dihydrofolate reductase gene in Chinese hamster ovary cells, amplified for the gene, by quantitating pyrimidine dimers with a specific UV-endonuclease. More than two thirds of the dimers had been removed from a 14.1 kb restriction fragment of the gene by 26 hr after irradiation (20 J/m2)...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90150-3
更新日期:1985-02-01 00:00:00
abstract::Oligodendrocyte-specific protein (OSP)/claudin-11 is a recently identified transmembrane protein found in CNS myelin and testis with unknown function. Herein we demonstrate that Osp null mice exhibit both neurological and reproductive deficits: CNS nerve conduction is slowed, hindlimb weakness is conspicuous, and male...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81553-6
更新日期:1999-12-10 00:00:00
abstract::Previous experiments have shown that mRNA translation in frog oocytes can be inhibited by the injection of a complementary antisense RNA. Here we explore the use of antisense RNAs to study the functions of localized maternal mRNAs during postfertilization development. While developmental abnormalities were observed in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90238-8
更新日期:1987-02-27 00:00:00
abstract::Chemical modifications of histones and DNA, such as histone methylation, histone acetylation, and DNA methylation, play critical roles in epigenetic gene regulation. Many of the enzymes that add or remove such chemical modifications are known, or might be suspected, to be sensitive to changes in intracellular metaboli...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2013.03.004
更新日期:2013-03-28 00:00:00
abstract::In prokaryotes, the toxin-antitoxin systems are thought to play important roles in growth regulation under stress conditions. In the E. coli MazE-MazF system, MazF toxin functions as an mRNA interferase cleaving mRNAs at ACA sequences to inhibit protein synthesis leading to cell growth arrest. Myxococcus xanthus is a ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.11.044
更新日期:2008-01-11 00:00:00
abstract::The recognition of specific DNA-binding sites by transcription factors is a critical yet poorly understood step in the control of gene expression. Members of the Hox family of transcription factors bind DNA by making nearly identical major groove contacts via the recognition helices of their homeodomains. In vivo spec...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.09.024
更新日期:2007-11-02 00:00:00
abstract::Toxins have evolved to target regions of membrane ion channels that underlie ligand binding, gating, or ion permeation, and have thus served as invaluable tools for probing channel structure and function. Here, we describe a peptide toxin from the Earth Tiger tarantula that selectively and irreversibly activates the c...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2010.03.052
更新日期:2010-05-28 00:00:00
abstract::During membrane fusion, the outer leaflets of the two membranes merge first, whereas the distal membrane leaflets remain separate until the opening of a fusion pore. This intermediate stage, called hemifusion, is a critical event shared by exocytosis, protein trafficking, and viral entry. ...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2005.10.015
更新日期:2005-11-04 00:00:00
abstract::For many scientific awards, such as Nobels and Laskers, the maximum number of recipients is three. This Rule of Three forces selection committees to make difficult decisions that increase the likelihood of singling out those individuals who open a new field and continue to lead it. The Rule of Three is reminiscent of ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.08.040
更新日期:2016-09-22 00:00:00
abstract::We have cloned the C. elegans cell death gene ced-3. A ced-3 transcript is most abundant during embryogenesis, the stage during which most programmed cell deaths occur. The predicted CED-3 protein shows similarity to human and murine interleukin-1 beta-converting enzyme and to the product of the mouse nedd-2 gene, whi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90485-9
更新日期:1993-11-19 00:00:00
abstract::Vanadate has been shown to inhibit phosphatases that remove phosphate groups from phosphotyrosine in cell-free systems. Addition of vanadate to the culture medium of NRK-1 cells resulted in a maximal 40-fold increase in the level of phosphotyrosine in cell protein. Also, vanadate induced transformation as evidenced by...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(85)80051-9
更新日期:1985-07-01 00:00:00
abstract::Extraction of glycerinated chicken skeletal muscle with 0.6 M potassium iodide leaves a framework of insoluble components within each muscle fiber. This framework is composed primarily of planes of in-register Z discs that have been thickened by the accumulation of material on both sides of each disc during extraction...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90051-x
更新日期:1978-12-01 00:00:00
abstract::xol-1 is the earliest-acting gene in the known hierarchy that controls C. elegans sex determination and dosage compensation. We show that the primary sex-determining signal (the X/A ratio) directs the choice of sexual fate by regulating xol-1 transcript levels: high xol-1 expression during gastrulation triggers male d...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90452-2
更新日期:1995-01-13 00:00:00
abstract::An easy manipulation of the commercial fluorescence microscope allows stereo pairs of pictures to be taken, which when examined with a stereo viewer, give a strong three-dimensional impression. The procedure is described in detail. Its use allows the documentation by immunofluorescence microscopy of the three-dimensio...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90234-9
更新日期:1978-07-01 00:00:00
abstract::Transcriptional regulation by the glucocorticoid receptor (GR) is mediated by hormone binding, receptor dimerization, and coactivator recruitment. Here, we report the crystal structure of the human GR ligand binding domain (LBD) bound to dexamethasone and a coactivator motif derived from the transcriptional intermedia...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(02)00817-6
更新日期:2002-07-12 00:00:00
abstract::Minimal hammerhead ribozymes have been characterized extensively by static and time-resolved crystallography as well as numerous biochemical analyses, leading to mutually contradictory mechanistic explanations for catalysis. We present the 2.2 A resolution crystal structure of a full-length Schistosoma mansoni hammerh...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.06.036
更新日期:2006-07-28 00:00:00
abstract::In this issue, Metzis et al., demonstrate that in the development of the central nervous system, patterning along the anterior-posterior axis precedes acquisition of neural identity. This contrasts with the prevailing view that neural identity comes first, providing a new window on the origins of the brain and spinal ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2018.10.040
更新日期:2018-11-01 00:00:00