The bacterial effector VopL organizes actin into filament-like structures.


:VopL is an effector protein from Vibrio parahaemolyticus that nucleates actin filaments. VopL consists of a VopL C-terminal domain (VCD) and an array of three WASP homology 2 (WH2) motifs. Here, we report the crystal structure of the VCD dimer bound to actin. The VCD organizes three actin monomers in a spatial arrangement close to that found in the canonical actin filament. In this arrangement, WH2 motifs can be modeled into the binding site of each actin without steric clashes. The data suggest a mechanism of nucleation wherein VopL creates filament-like structures, organized by the VCD with monomers delivered by the WH2 array, that can template addition of new subunits. Similarities with Arp2/3 complex and formin proteins suggest that organization of monomers into filament-like structures is a general and central feature of actin nucleation.






Zahm JA,Padrick SB,Chen Z,Pak CW,Yunus AA,Henry L,Tomchick DR,Chen Z,Rosen MK




Has Abstract


2013-10-10 00:00:00
















  • Nine introns with conserved boundary sequences in the Euglena gracilis chloroplast ribulose-1,5-bisphosphate carboxylase gene.

    abstract::The single, chloroplast encoded gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL) from Euglena gracilis is found to contain nine intervening sequences. The intervening sequences were identified by heteroduplex analysis between Euglena rbcL and the non-intron-containing rbcL from Spin...


    pub_type: 杂志文章


    authors: Koller B,Gingrich JC,Stiegler GL,Farley MA,Delius H,Hallick RB

    更新日期:1984-02-01 00:00:00

  • Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.

    abstract::Conversion of glial cells into functional neurons represents a potential therapeutic approach for replenishing neuronal loss associated with neurodegenerative diseases and brain injury. Previous attempts in this area using expression of transcription factors were hindered by the low conversion efficiency and failure o...


    pub_type: 杂志文章


    authors: Zhou H,Su J,Hu X,Zhou C,Li H,Chen Z,Xiao Q,Wang B,Wu W,Sun Y,Zhou Y,Tang C,Liu F,Wang L,Feng C,Liu M,Li S,Zhang Y,Xu H,Yao H,Shi L,Yang H

    更新日期:2020-04-30 00:00:00

  • p53 and its 14 kDa C-terminal domain recognize primary DNA damage in the form of insertion/deletion mismatches.

    abstract::Insertion/deletion (IDL) mismatches in DNA are lesions consisting of extra bases on one strand. Here, the binding of p53 and its 14 kDa C-terminal domain to DNAs containing one or three 3-cytosine IDL mismatches was examined. Electron microscopy showed that both p53 forms bound predominantly as tetramers at the lesion...


    pub_type: 杂志文章


    authors: Lee S,Elenbaas B,Levine A,Griffith J

    更新日期:1995-06-30 00:00:00

  • Positive regulation of the cAMP-responsive activator CREM by the p70 S6 kinase: an alternative route to mitogen-induced gene expression.

    abstract::Activation of the adenylyl cyclase signaling pathway elicits the induction of genes via activators binding to cAMP-responsive elements (CREs). Nuclear factor CRE modulator (CREM) is activated by PKA-mediated phosphorylation on a serine at position 117. We show that Ser-117 is also phosphorylated by the mitogen-activat...


    pub_type: 杂志文章


    authors: de Groot RP,Ballou LM,Sassone-Corsi P

    更新日期:1994-10-07 00:00:00

  • Stabilizing HIF to Ameliorate Anemia.

    abstract::Erythropoietin (EPO) production in the kidney is regulated by the oxygen-sensing transcription factor HIF-1α, which is degraded under normoxic conditions by HIF-prolyl hydroxylase (HIF-PHD). Inhibition of HIF-PHD by roxadustat leads to increased EPO production, better iron absorption, and amelioration of anemia in chr...


    pub_type: 杂志文章


    authors: Voit RA,Sankaran VG

    更新日期:2020-01-09 00:00:00

  • Alleles of the APC gene: an attenuated form of familial polyposis.

    abstract::An attenuated form of familial adenomatous polyposis coli, AAPC, causes relatively few colonic polyps, but still carries a significant risk of colon cancer. The mutant alleles responsible for this attenuated phenotype have been mapped in several families to the adenomatous polyposis coli (APC) locus on human chromosom...


    pub_type: 杂志文章


    authors: Spirio L,Olschwang S,Groden J,Robertson M,Samowitz W,Joslyn G,Gelbert L,Thliveris A,Carlson M,Otterud B

    更新日期:1993-12-03 00:00:00

  • A novel glycosphingolipid that may participate in embryo inversion in Volvox carteri.

    abstract::Mouse monoclonal antibody 4-I-244 detects a developmentally regulated antigen in embryos of Volvox carteri and inhibits specifically the morphogenetic process of inversion (the process by which the embryo turns inside out). Antigen 4-I-244 was chemically characterized as a complex phytosphingolipid containing the neut...


    pub_type: 杂志文章


    authors: Wenzl S,Sumper M

    更新日期:1986-08-15 00:00:00

  • The 5' terminal structure of the methylated mRNA synthesized in vitro by vesicular stomatitis virus.

    abstract::The 5' terminal structure of the mRNA synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus in the presence of S-adenosyl-L-methione consists of 7-methyl guanosine linked to 2'-O-methyl adenosine through a 5'-5' pyrophosphate bond as m7G(5')ppp(5')A-m-p ... The alpha and beta phosp...


    pub_type: 杂志文章


    authors: Abraham G,Rhodes DP,Banerjee AK

    更新日期:1975-05-01 00:00:00

  • A unique cap(m7GpppXm)-dependent influenza virion endonuclease cleaves capped RNAs to generate the primers that initiate viral RNA transcription.

    abstract::We propose a mechanism for the priming of influenza viral RNA transcription by capped RNAs in which specific 5'-terminal fragments are cleaved from the capped RNAs by a virion-associated endonuclease. These fragments would serve as the actual primers for the initiation of transcription by the initial incorporation by ...


    pub_type: 杂志文章


    authors: Plotch SJ,Bouloy M,Ulmanen I,Krug RM

    更新日期:1981-03-01 00:00:00

  • Structure and sequence of the cellular gene homologous to the RSV src gene and the mechanism for generating the transforming virus.

    abstract::We determined the nucleotide sequences of all coding regions and a significant part of the flanking regions of the chicken c-src gene, which is a cellular homolog of the v-src gene of Rous sarcoma virus. The c-src gene consists of 12 exons; the boundaries of the exons were determined by assuming that the amino acid se...


    pub_type: 杂志文章


    authors: Takeya T,Hanafusa H

    更新日期:1983-03-01 00:00:00

  • Lateral and axial signals involved in avian somite patterning: a role for BMP4.

    abstract::In vertebrates, muscles of the limbs and body wall derive from the lateral compartment of the embryonic somites, and axial muscles derive from the medial compartment. Whereas the mechanisms that direct patterning of somites along the dorsoventral axis are beginning to be understood, little is known about the tissue in...


    pub_type: 杂志文章


    authors: Pourquié O,Fan CM,Coltey M,Hirsinger E,Watanabe Y,Bréant C,Francis-West P,Brickell P,Tessier-Lavigne M,Le Douarin NM

    更新日期:1996-02-09 00:00:00

  • Follicular dendritic cells emerge from ubiquitous perivascular precursors.

    abstract::The differentiation of follicular dendritic cells (FDC) is essential to the remarkable microanatomic plasticity of lymphoid follicles. Here we show that FDC arise from ubiquitous perivascular precursors (preFDC) expressing platelet-derived growth factor receptor β (PDGFRβ). PDGFRβ-Cre-driven reporter gene recombinatio...


    pub_type: 杂志文章


    authors: Krautler NJ,Kana V,Kranich J,Tian Y,Perera D,Lemm D,Schwarz P,Armulik A,Browning JL,Tallquist M,Buch T,Oliveira-Martins JB,Zhu C,Hermann M,Wagner U,Brink R,Heikenwalder M,Aguzzi A

    更新日期:2012-07-06 00:00:00

  • Cellular motility driven by assembly and disassembly of actin filaments.

    abstract::Motile cells extend a leading edge by assembling a branched network of actin filaments that produces physical force as the polymers grow beneath the plasma membrane. A core set of proteins including actin, Arp2/3 complex, profilin, capping protein, and ADF/cofilin can reconstitute the process in vitro, and mathematica...


    pub_type: 杂志文章,评审


    authors: Pollard TD,Borisy GG

    更新日期:2003-02-21 00:00:00

  • Production of a DPP activity gradient in the early Drosophila embryo through the opposing actions of the SOG and TLD proteins.

    abstract::During early Drosophila embryogenesis, several zygotic gene products act to establish a posttranscriptional activity gradient of the morphogen DPP. Among these molecules, Tolloid, a putative metalloprotease related to BMP-1, enhances DPP function, while SOG, an ortholog of the Xenopus organizer Chordin, inhibits DPP f...


    pub_type: 杂志文章


    authors: Marqués G,Musacchio M,Shimell MJ,Wünnenberg-Stapleton K,Cho KW,O'Connor MB

    更新日期:1997-10-31 00:00:00

  • Sequence from early region of polyoma virus DNA containing viral replication origin and encoding small, middle and (part of) large T antigens.

    abstract::The sequence of about one third of the polyoma virus genome is presented. This sequence covers the origin of replication of two large plaque strains (A2 and A3) of polyoma virus. The two strains differ by 11 bp in the origin region. A model for replication is suggested. The sequence probably also covers the entire cod...


    pub_type: 杂志文章


    authors: Soeda E,Arrand JR,Smolar N,Griffin BE

    更新日期:1979-06-01 00:00:00

  • Recognition sequences of repressor and polymerase in the operators of bacteriophage lambda.

    abstract::Nucleotide sequences in two wild-type and six mutant operators in the DNA of phage lambda are compared. Strikingly similar 17 base pair units are found which we identify as the repressor binding sites. Each operator contains multiple repressor binding sites separated by A-T rich spacers. Elements of 2 fold rotational ...


    pub_type: 杂志文章


    authors: Maniatis T,Ptashne M,Backman K,Kield D,Flashman S,Jeffrey A,Maurer R

    更新日期:1975-06-01 00:00:00

  • It takes a (dysfunctional) village to raise a tumor.

    abstract::The tumor microenvironment is known to play an important role in tumorigenesis. In this issue, Yang et al. (2008) demonstrate that mast cells heterozygous for the Nf1 gene promote the growth of neurofibromas in a mouse model of neurofibromatosis and that genetic and pharmacological inhibition of these cells is suffici...


    pub_type: 评论,杂志文章


    authors: Reilly KM,Van Dyke T

    更新日期:2008-10-31 00:00:00

  • Accelerated evolution of nervous system genes in the origin of Homo sapiens.

    abstract::Human evolution is characterized by a dramatic increase in brain size and complexity. To probe its genetic basis, we examined the evolution of genes involved in diverse aspects of nervous system biology. We found that these genes display significantly higher rates of protein evolution in primates than in rodents. Impo...


    pub_type: 杂志文章


    authors: Dorus S,Vallender EJ,Evans PD,Anderson JR,Gilbert SL,Mahowald M,Wyckoff GJ,Malcom CM,Lahn BT

    更新日期:2004-12-29 00:00:00

  • The diabetes susceptibility gene Clec16a regulates mitophagy.

    abstract::Clec16a has been identified as a disease susceptibility gene for type 1 diabetes, multiple sclerosis, and adrenal dysfunction, but its function is unknown. Here we report that Clec16a is a membrane-associated endosomal protein that interacts with E3 ubiquitin ligase Nrdp1. Loss of Clec16a leads to an increase in the N...


    pub_type: 杂志文章


    authors: Soleimanpour SA,Gupta A,Bakay M,Ferrari AM,Groff DN,Fadista J,Spruce LA,Kushner JA,Groop L,Seeholzer SH,Kaufman BA,Hakonarson H,Stoffers DA

    更新日期:2014-06-19 00:00:00

  • Drosophila cytokine unpaired 2 regulates physiological homeostasis by remotely controlling insulin secretion.

    abstract::In Drosophila, the fat body (FB), a functional analog of the vertebrate adipose tissue, is the nutrient sensor that conveys the nutrient status to the insulin-producing cells (IPCs) in the fly brain to release Drosophila insulin-like peptides (Dilps). Dilp secretion in turn regulates energy balance and promotes system...


    pub_type: 杂志文章


    authors: Rajan A,Perrimon N

    更新日期:2012-09-28 00:00:00

  • Disconnected: a locus required for neuronal pathway formation in the visual system of Drosophila.

    abstract::Mutations at the X-linked disconnected locus of D. melanogaster lead to the failure of adult photoreceptor axons to innervate their target cells in the developing optic lobes of the third instar larva, resulting in flies that have rudimentary optic ganglia. The cascade of epigenetic events leading to the adult disconn...


    pub_type: 杂志文章


    authors: Steller H,Fischbach KF,Rubin GM

    更新日期:1987-09-25 00:00:00

  • Adipose Tissue Macrophage-Derived Exosomal miRNAs Can Modulate In Vivo and In Vitro Insulin Sensitivity.

    abstract::MiRNAs are regulatory molecules that can be packaged into exosomes and secreted from cells. Here, we show that adipose tissue macrophages (ATMs) in obese mice secrete miRNA-containing exosomes (Exos), which cause glucose intolerance and insulin resistance when administered to lean mice. Conversely, ATM Exos obtained f...


    pub_type: 杂志文章


    authors: Ying W,Riopel M,Bandyopadhyay G,Dong Y,Birmingham A,Seo JB,Ofrecio JM,Wollam J,Hernandez-Carretero A,Fu W,Li P,Olefsky JM

    更新日期:2017-10-05 00:00:00

  • A cyclin-dependent kinase inhibitor, Dacapo, is necessary for timely exit from the cell cycle during Drosophila embryogenesis.

    abstract::In a screen for genes that interact with the Rap1 GTPase, we have identified a Drosophila gene, dacapo (dap), which is a member of the p21/p27 family of cdk inhibitors. Unlike mammalian cdk inhibitors studied to date, dap is essential for normal embryonic development. Dacapo inhibits cyclin-cdk activity in vitro. Over...


    pub_type: 杂志文章


    authors: de Nooij JC,Letendre MA,Hariharan IK

    更新日期:1996-12-27 00:00:00

  • The cryb mutation identifies cryptochrome as a circadian photoreceptor in Drosophila.

    abstract::A new rhythm mutation was isolated based on its elimination of per-controlled luciferase cycling. Levels of period or timeless clock gene products in the mutant are flat in daily light-dark cycles or constant darkness (although PER and TIM oscillate normally in temperature cycles). Consistent with the fact that light ...


    pub_type: 杂志文章


    authors: Stanewsky R,Kaneko M,Emery P,Beretta B,Wager-Smith K,Kay SA,Rosbash M,Hall JC

    更新日期:1998-11-25 00:00:00

  • The three-dimensional structure of HLA-B27 at 2.1 A resolution suggests a general mechanism for tight peptide binding to MHC.

    abstract::Cell surface complexes of class I MHC molecules and bound peptide antigens serve as specific recognition elements controlling the cytotoxic immune response. The 2.1 A structure of the human class I MHC molecule HLA-B27 provides a detailed composite image of a co-crystallized collection of HLA-B27-bound peptides, indic...


    pub_type: 杂志文章


    authors: Madden DR,Gorga JC,Strominger JL,Wiley DC

    更新日期:1992-09-18 00:00:00

  • A Single Transcription Factor Drives Toxoplasma gondii Differentiation.

    abstract::Microbes that cause persistent infections (e.g., herpes viruses) do so by switching from fast-growing lytic states to slow-growing latent states. Waldman et al. have identified a single transcription factor that governs the switch between the lytic and latent forms of Toxoplasma gondii, a parasite that causes a persis...


    pub_type: 评论,杂志文章


    authors: Kochanowsky JA,Koshy AA

    更新日期:2020-01-23 00:00:00

  • Rickettsia Sca4 Reduces Vinculin-Mediated Intercellular Tension to Promote Spread.

    abstract::Spotted fever group (SFG) rickettsiae are human pathogens that infect cells in the vasculature. They disseminate through host tissues by a process of cell-to-cell spread that involves protrusion formation, engulfment, and vacuolar escape. Other bacterial pathogens rely on actin-based motility to provide a physical for...


    pub_type: 杂志文章


    authors: Lamason RL,Bastounis E,Kafai NM,Serrano R,Del Álamo JC,Theriot JA,Welch MD

    更新日期:2016-10-20 00:00:00

  • Presence of histone H1 on an active Balbiani ring gene.

    abstract::We have investigated whether histone H1 is present on active Balbiani ring genes in the salivary glands of Chironomus tentans using immunoelectron microscopy. The genes were studied in two activity states: maximally activated genes with a fully extended template and repressed genes in a 30 nm fiber conformation. Histo...


    pub_type: 杂志文章


    authors: Ericsson C,Grossbach U,Björkroth B,Daneholt B

    更新日期:1990-01-12 00:00:00

  • Making sense of transcription networks.

    abstract::When transcription regulatory networks are compared among distantly related eukaryotes, a number of striking similarities are observed: a larger-than-expected number of genes, extensive overlapping connections, and an apparently high degree of functional redundancy. It is often assumed that the complexity of these net...


    pub_type: 杂志文章,评审


    authors: Sorrells TR,Johnson AD

    更新日期:2015-05-07 00:00:00

  • Critical role of amyloid-like oligomers of Drosophila Orb2 in the persistence of memory.

    abstract::A long-standing question in the study of long-term memory is how a memory trace persists for years when the proteins that initiated the process turn over and disappear within days. Previously, we postulated that self-sustaining amyloidogenic oligomers of cytoplasmic polyadenylation element-binding protein (CPEB) provi...


    pub_type: 杂志文章


    authors: Majumdar A,Cesario WC,White-Grindley E,Jiang H,Ren F,Khan MR,Li L,Choi EM,Kannan K,Guo F,Unruh J,Slaughter B,Si K

    更新日期:2012-02-03 00:00:00