Abstract:
:Reversible protein phosphorylation is a signaling mechanism involved in all cellular processes. To create a systems view of the signaling apparatus in budding yeast, we generated an epistatic miniarray profile (E-MAP) comprised of 100,000 pairwise, quantitative genetic interactions, including virtually all protein and small-molecule kinases and phosphatases as well as key cellular regulators. Quantitative genetic interaction mapping reveals factors working in compensatory pathways (negative genetic interactions) or those operating in linear pathways (positive genetic interactions). We found an enrichment of positive genetic interactions between kinases, phosphatases, and their substrates. In addition, we assembled a higher-order map from sets of three genes that display strong interactions with one another: triplets enriched for functional connectivity. The resulting network view provides insights into signaling pathway regulation and reveals a link between the cell-cycle kinase, Cak1, the Fus3 MAP kinase, and a pathway that regulates chromatin integrity during transcription by RNA polymerase II.
journal_name
Celljournal_title
Cellauthors
Fiedler D,Braberg H,Mehta M,Chechik G,Cagney G,Mukherjee P,Silva AC,Shales M,Collins SR,van Wageningen S,Kemmeren P,Holstege FC,Weissman JS,Keogh MC,Koller D,Shokat KM,Krogan NJdoi
10.1016/j.cell.2008.12.039subject
Has Abstractpub_date
2009-03-06 00:00:00pages
952-63issue
5eissn
0092-8674issn
1097-4172pii
S0092-8674(09)00002-6journal_volume
136pub_type
杂志文章相关文献
CELL文献大全abstract::Ku is a heterodimeric DNA end binding complex composed of 70 and 86 kDa subunits. Here, we show that Ku86 is essential for normal V(D)J recombination in vivo, as Ku86-deficient mice are severely defective for formation of coding joints. Unlike severe combined immunodeficient (scid) mice, Ku86-deficient mice are also d...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80111-7
更新日期:1996-08-09 00:00:00
abstract::Unique among leukocytes, neutrophils follow daily cycles of release from and migration back into the bone marrow, where they are eliminated. Because removal of dying cells generates homeostatic signals, we explored whether neutrophil elimination triggers circadian events in the steady state. Here, we report that the h...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.04.040
更新日期:2013-05-23 00:00:00
abstract::The dorsal raphe nucleus (DRN) is an important brain area for body-weight regulation. In this issue of Cell, Nectow et al. uncover cell-type-specific neural circuitry and pharmacology for appetite control within the DRN. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2017.07.017
更新日期:2017-07-27 00:00:00
abstract::The L7/12 stalk of the large subunit of bacterial ribosomes encompasses protein L10 and multiple copies of L7/12. We present crystal structures of Thermotoga maritima L10 in complex with three L7/12 N-terminal-domain dimers, refine the structure of an archaeal L10E N-terminal domain on the 50S subunit, and identify th...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2005.04.015
更新日期:2005-07-01 00:00:00
abstract::We employed gene targeting to study H2AX, a histone variant phosphorylated in chromatin surrounding DNA double-strand breaks. Mice deficient for both H2AX and p53 (H(delta/delta)P(-/-)) rapidly developed immature T and B lymphomas and solid tumors. Moreover, H2AX haploinsufficiency caused genomic instability in normal...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00566-x
更新日期:2003-08-08 00:00:00
abstract::Kinetochores connect centromeric nucleosomes with mitotic-spindle microtubules through conserved, cross-interacting protein subassemblies. In budding yeast, the heterotetrameric MIND complex (Mtw1, Nnf1, Nsl1, Dsn1), ortholog of the metazoan Mis12 complex, joins the centromere-proximal components, Mif2 and COMA, with ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.10.011
更新日期:2016-11-03 00:00:00
abstract::The trk tyrosine kinase proto-oncogene product gp140prototrk binds nerve growth factor (NGF) and is rapidly and selectively activated by this neurotrophic factor. To determine whether gp140prototrk is involved in transducing a functional NGF signal, PC12 cell mutants (PC12nnr) deficient in high affinity NGF binding an...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90441-z
更新日期:1991-09-06 00:00:00
abstract::Initiation of tumorigenesis in mouse skin can be accomplished by mutagenesis of the H-ras gene by treatment with chemical carcinogens. A mouse model system has been developed to study the additional genetic events that take place during tumor progression. Skin carcinomas were induced in F1 hybrid mice exhibiting restr...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90523-h
更新日期:1990-05-04 00:00:00
abstract::BRCA1 interacts in vivo with a novel protein, BACH1, a member of the DEAH helicase family. BACH1 binds directly to the BRCT repeats of BRCA1. A BACH1 derivative, bearing a mutation in a residue that was essential for catalytic function in other helicases, interfered with normal double-strand break repair in a manner t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00304-x
更新日期:2001-04-06 00:00:00
abstract::Behavioral expression of food-associated memory in fruit flies is constrained by satiety and promoted by hunger, suggesting an influence of motivational state. Here, we identify a neural mechanism that integrates the internal state of hunger and appetitive memory. We show that stimulation of neurons that express neuro...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.08.035
更新日期:2009-10-16 00:00:00
abstract::Affordable genome sequencing technologies promise to revolutionize the field of human genetics by enabling comprehensive studies that interrogate all classes of genome variation, genome-wide, across the entire allele frequency spectrum. Ongoing projects worldwide are sequencing many thousands-and soon millions-of huma...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2019.02.032
更新日期:2019-03-21 00:00:00
abstract::Two DNA sequences that reduce mitotic fidelity of chromosome transmission have been identified: MIF1 and MIF2. MIF1 is a unique sequence located on the right arm of chromosome XII that stimulates loss and recombination for both chromosomes V and VII when present in a high copy number plasmid. MIF1 is not essential for...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90484-8
更新日期:1986-01-17 00:00:00
abstract::Negative selection with FUdR produced revertants from the transformed rat line 14B, which contains one insertion of the SV40 viral genome (Botchan, Topp and Sambrook, 1976). 14B contains nuclear T antigen, grows to a high density, grows in low serum and is anchorage-independent. The revertants fall into three classes ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90134-4
更新日期:1978-01-01 00:00:00
abstract::Antibodies capable of neutralizing divergent influenza A viruses could form the basis of a universal vaccine. Here, from subjects enrolled in an H5N1 DNA/MIV-prime-boost influenza vaccine trial, we sorted hemagglutinin cross-reactive memory B cells and identified three antibody classes, each capable of neutralizing di...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.06.043
更新日期:2016-07-28 00:00:00
abstract::PTEN is a frequently mutated tumor suppressor gene that opposes the PI3K/AKT pathway through dephosphorylation of phosphoinositide-3,4,5-triphosphate. Recently, nuclear compartmentalization of PTEN was found as a key component of its tumor-suppressive activity; however its nuclear function remains poorly defined. Here...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2010.12.020
更新日期:2011-01-21 00:00:00
abstract::The science advisor to the US president has the opportunity to influence how scientific research is supported by the federal government, as well as the ways in which science and technology can be applied to meet the needs of society. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.11.024
更新日期:2009-11-25 00:00:00
abstract::Using a defined RNA polymerase II (pol II) transcription system, we have investigated the roles of basal factors at discrete stages during the transcription cycle (e.g., initiation, promoter clearance, and transcript elongation). Abortive initiation assays revealed that TATA-binding protein, transcription factors TFII...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90242-9
更新日期:1994-04-08 00:00:00
abstract::Neurons make networks of intercellular connections. Many other cells also send out long cellular extensions and "touch" other cells far away. The extensions are intriguing, but what do they do? Possible rationales for this cell behavior include: (1) forming precise binary connections with the possibility of forming mo...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/s0092-8674(03)00156-9
更新日期:2003-03-07 00:00:00
abstract::Transduction of cellular genes by retroviruses requires two recombinations: one to form the left-hand junction between cellular gene and viral genome, the other to form the right-hand junction. Previous findings raised the possibility that the right-hand recombination might use RNA molecules as intermediates. We now p...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90016-4
更新日期:1986-03-28 00:00:00
abstract::The peripheral nervous system has remarkable regenerative capacities in that it can repair a fully cut nerve. This requires Schwann cells to migrate collectively to guide regrowing axons across a 'bridge' of new tissue, which forms to reconnect a severed nerve. Here we show that blood vessels direct the migrating cord...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.07.021
更新日期:2015-08-27 00:00:00
abstract::Even a simple sensory stimulus can elicit distinct innate behaviors and sequences. During sensorimotor decisions, competitive interactions among neurons that promote distinct behaviors must ensure the selection and maintenance of one behavior, while suppressing others. The circuit implementation of these competitive i...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.09.009
更新日期:2016-10-20 00:00:00
abstract::Alzheimer's disease (AD) is a genetically heterogeneous disorder characterized by early hippocampal atrophy and cerebral amyloid-beta (Abeta) peptide deposition. Using TissueInfo to screen for genes preferentially expressed in the hippocampus and located in AD linkage regions, we identified a gene on 10q24.33 that we ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2008.05.048
更新日期:2008-06-27 00:00:00
abstract::The double-stranded RNA-activated protein kinase (PKR) was originally identified as a sensor of virus infection, but its function in the brain remains unknown. Here, we report that the lack of PKR enhances learning and memory in several behavioral tasks while increasing network excitability. In addition, loss of PKR i...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.11.029
更新日期:2011-12-09 00:00:00
abstract::Dopamine plays an important role in the etiology of schizophrenia, and D2 class dopamine receptors are the best-established target of antipsychotic drugs. Here we show that D2 class-receptor-mediated Akt regulation involves the formation of signaling complexes containing beta-arrestin 2, PP2A, and Akt. beta-arrestin 2...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2005.05.012
更新日期:2005-07-29 00:00:00
abstract::A clonally diverse anticipatory repertoire in which each lymphocyte bears a unique antigen receptor is the central feature of the adaptive immune system that evolved in our vertebrate ancestors. The survival advantage gained through adding this type of adaptive immune system to a pre-existing innate immune system led ...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2006.02.001
更新日期:2006-02-24 00:00:00
abstract::Eukaryotic translation initiation requires coordinated assembly of a remarkable array of initiation factors onto the small ribosomal subunit to select an appropriate mRNA start codon. Studies from Erzberger et al. and Hussain et al. bring new insights into this mechanism by looking at early and late initiation interme...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2014.10.005
更新日期:2014-10-23 00:00:00
abstract::Enteropathogenic E. coli (EPEC) belongs to a group of bacterial pathogens that induce epithelial cell actin rearrangements resulting in pedestal formation beneath adherent bacteria. This requires the secretion of specific virulence proteins needed for signal transduction and intimate adherence. EPEC interaction induce...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80437-7
更新日期:1997-11-14 00:00:00
abstract::The rps12 gene codes for chloroplast ribosomal protein S12. In the tobacco chloroplast genome (156 kbp circular DNA), exons II and III of this gene are separated by an intron of 536 bases and are present in two copies in the inverted repeat region, while exon I is located in the large single copy region at a distance ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90361-8
更新日期:1987-01-16 00:00:00
abstract::A mutation in any one of eight spoO genes of Bacillus subtilis blocks the process of spore formation at its earliest stage. To investigate how the products of the spoO genes may be involved in developmental gene expression, we fused the lacZ gene of E. coli to spoVG, a sporulation gene whose induction at the onset of ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90230-1
更新日期:1983-11-01 00:00:00
abstract::Most human epithelial tumors harbor numerous alterations, making it difficult to predict which genes are required for tumor survival. To systematically identify cancer dependencies, we analyzed 501 genome-scale loss-of-function screens performed in diverse human cancer cell lines. We developed DEMETER, an analytical f...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.06.010
更新日期:2017-07-27 00:00:00