Abstract:
:E-cadherin is a major homophilic cell-cell adhesion molecule that inhibits motility of individual cells on matrix. However, its contribution to migration of cells through cell-rich tissues is less clear. We developed an in vivo sensor of mechanical tension across E-cadherin molecules, which we combined with cell-type-specific RNAi, photoactivatable Rac, and morphodynamic profiling, to interrogate how E-cadherin contributes to collective migration of cells between other cells. Using the Drosophila ovary as a model, we found that adhesion between border cells and their substrate, the nurse cells, functions in a positive feedback loop with Rac and actin assembly to stabilize forward-directed protrusion and directionally persistent movement. Adhesion between individual border cells communicates direction from the lead cell to the followers. Adhesion between motile cells and polar cells holds the cluster together and polarizes each individual cell. Thus, E-cadherin is an integral component of the guidance mechanisms that orchestrate collective chemotaxis in vivo.
journal_name
Celljournal_title
Cellauthors
Cai D,Chen SC,Prasad M,He L,Wang X,Choesmel-Cadamuro V,Sawyer JK,Danuser G,Montell DJdoi
10.1016/j.cell.2014.03.045subject
Has Abstractpub_date
2014-05-22 00:00:00pages
1146-59issue
5eissn
0092-8674issn
1097-4172pii
S0092-8674(14)00473-5journal_volume
157pub_type
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