Abstract：
:Previous experiments have shown that mRNA translation in frog oocytes can be inhibited by the injection of a complementary antisense RNA. Here we explore the use of antisense RNAs to study the functions of localized maternal mRNAs during postfertilization development. While developmental abnormalities were observed in injected fertilized eggs, these abnormalities could not be attributed to the antisense RNA since they were induced at a similar frequency in control embryos. Biochemical tests show that the injected antisense RNA does not form stable hybrids in vivo with its complementary endogenous mRNA. In addition, a novel activity that unwinds RNA:RNA duplexes was found. This activity exists at high levels in eggs and early embryos and is absent or very much diminished in oocytes and late blastula embryos. These results suggest that antisense RNAs may be of limited use in studying the functions of maternal RNAs in Xenopus.
journal_name
Celljournal_title
Cellauthors
Rebagliati MR,Melton DAdoi
10.1016/0092-8674(87)90238-8subject
Has Abstractpub_date
1987-02-27 00:00:00pages
599-605issue
4eissn
0092-8674issn
1097-4172pii
0092-8674(87)90238-8journal_volume
48pub_type
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