Abstract：
:The spindle pole body (SPB) is the microtubule organizing center (MTOC) in the yeast Saccharomyces that plays a pivotal role in such diverse processes as mitosis, budding, and mating. We have used cryoelectron microscopy and image processing to study the structure of isolated diploid SPBs. We show that SPBs are present in two lateral-size classes, sharing a similar vertical architecture comprised of six major layers. Tomographic reconstructions of heparin-stripped SPBs reveal a central hexagonally packed layer. Overexpression of Spc42p results in the growth of a similar layer, forming a crystal that encircles the SPB. Hence, the SPB is an MTOC that utilizes crystallographic packing of subunits in its construction.
journal_name
Celljournal_title
Cellauthors
Bullitt E,Rout MP,Kilmartin JV,Akey CWdoi
10.1016/s0092-8674(00)80295-0subject
Has Abstractpub_date
1997-06-27 00:00:00pages
1077-86issue
7eissn
0092-8674issn
1097-4172pii
S0092-8674(00)80295-0journal_volume
89pub_type
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