Abstract：
:Portions of the nascent chain are exposed to the lumen, the cytosol, or neither at different stages during the cotranslational integration of a protein into the ER membrane, as shown by compartment-specific collisional quenching of fluorophores incorporated into the polypeptide. The opening or closing of each end of the aqueous translocon pore is tightly controlled and occurs in a sequence that does not compromise the membrane's permeability barrier. Surprisingly, these structural changes at the membrane are effected by the transmembrane segment in the nascent protein from inside the ribosome. Thus, the ribosome, not the translocon, first recognizes the transmembrane segment and triggers long-range structural changes at the translocon that may be involved in shifting its function from translocation to integration.
journal_name
Celljournal_title
Cellauthors
Liao S,Lin J,Do H,Johnson AEdoi
10.1016/s0092-8674(00)80311-6subject
Has Abstractpub_date
1997-07-11 00:00:00pages
31-41issue
1eissn
0092-8674issn
1097-4172pii
S0092-8674(00)80311-6journal_volume
90pub_type
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