Molecular cloning and sequence analysis of a haploid expressed gene encoding t complex polypeptide 1.


:The mouse t haplotypes show defects in spermatogenesis attributed to multiple loci on chromosome 17. We have cloned the gene for an abundant testicular germ cell protein, t complex polypeptide 1, which has a variant form in t haplotypes, TCP-1A. A cDNA clone, pB1.4, which hybridizes to a 19S mRNA that is abundant in haploid cells during mouse spermatogenesis, derives from the 3' end of the mRNA encoding TCP-1B. The Tcp-1 gene appears to be a member of a novel gene family and shows multiple changes between the predicted amino acid sequences of TCP-1B and TCP-1A. An additional Taq1 site is created by a T to C transition in the predicted open reading frame of the Tcp-1a gene. The resultant RFLP has allowed typing of the Tcp-1 gene cluster in 54 complete and partial t haplotype chromosomes. DNA sequence comparison of the Tcp-1 genes suggests that the t haplotype chromosome arose within the genus Mus more than one million years ago.






Willison KR,Dudley K,Potter J




Has Abstract


1986-03-14 00:00:00
















  • Cyclic-AMP-dependent protein kinase type II is associated with the Golgi complex and with centrosomes.

    abstract::The subcellular distribution of the type II enzyme of cAMP-dependent protein kinase (cAMP-dPK II) in epithelial and fibroblastic cells was determined by indirect immunofluorescence microscopy. In interphase cells both regulatory (R II) and catalytic (C) subunits were concentrated in a perinuclear area. By comparison o...


    pub_type: 杂志文章


    authors: Nigg EA,Schäfer G,Hilz H,Eppenberger HM

    更新日期:1985-07-01 00:00:00

  • YDJ1p facilitates polypeptide translocation across different intracellular membranes by a conserved mechanism.

    abstract::The role of S. cerevisiae YDJ1 protein (YDJ1p) in polypeptide translocation across membranes has been examined. A conditional ydj1 mutant strain (ydj1-151TS) is defective for import of several polypeptides into mitochondria and alpha factor into the endoplasmic reticulum at 37 degrees C. These defects are suppressed b...


    pub_type: 杂志文章


    authors: Caplan AJ,Cyr DM,Douglas MG

    更新日期:1992-12-24 00:00:00

  • Tracing the retrograde route in protein trafficking.

    abstract::Retrograde transport, in which proteins and lipids are shuttled between endosomes and biosynthetic/secretory compartments such as the Golgi apparatus, is crucial for a diverse range of cellular functions. Mechanistic studies that explore the molecular machinery involved in this retrograde trafficking route are sheddin...


    pub_type: 杂志文章,评审


    authors: Johannes L,Popoff V

    更新日期:2008-12-26 00:00:00

  • Parafibromin/Hyrax activates Wnt/Wg target gene transcription by direct association with beta-catenin/Armadillo.

    abstract::The Wnt pathway controls cell fates, tissue homeostasis, and cancer. Its activation entails the association of beta-catenin with nuclear TCF/LEF proteins and results in transcriptional activation of target genes. The mechanism by which nuclear beta-catenin controls transcription is largely unknown. Here we genetically...


    pub_type: 杂志文章


    authors: Mosimann C,Hausmann G,Basler K

    更新日期:2006-04-21 00:00:00

  • A Smad transcriptional corepressor.

    abstract::Following TGFbeta receptor-mediated phosphorylation and association with Smad4, Smad2 moves into the nucleus, binds to target promoters in association with DNA-binding cofactors, and recruits coactivators such as p300/CBP to activate transcription. We identified the homeodomain protein TGIF as a Smad2-binding protein ...


    pub_type: 杂志文章


    authors: Wotton D,Lo RS,Lee S,Massagué J

    更新日期:1999-04-02 00:00:00

  • Endophilin mutations block clathrin-mediated endocytosis but not neurotransmitter release.

    abstract::We have identified mutations in Drosophila endophilin to study its function in vivo. Endophilin is required presynaptically at the neuromuscular junction, and absence of Endophilin dramatically impairs endocytosis in vivo. Mutant larvae that lack Endophilin fail to take up FM1-43 dye in synaptic boutons, indicating an...


    pub_type: 杂志文章


    authors: Verstreken P,Kjaerulff O,Lloyd TE,Atkinson R,Zhou Y,Meinertzhagen IA,Bellen HJ

    更新日期:2002-04-05 00:00:00

  • Pattern recognition receptors: doubling up for the innate immune response.

    abstract::Antigen presenting cells (macrophages and dendritic cells) express pattern recognition molecules that are thought to recognize foreign ligands during early phases of the immune response. The best known of these are probably the Toll-like receptors, but a number of other receptors are also involved. Several of these re...


    pub_type: 杂志文章,评审


    authors: Gordon S

    更新日期:2002-12-27 00:00:00

  • Synaptic transmission persists in synaptotagmin mutants of Drosophila.

    abstract::Synaptotagmin is one of the major integral membrane proteins of synaptic vesicles. It has been postulated to dock vesicles to their release sites, to act as the Ca2+ sensor for the release process, and to be a fusion protein during exocytosis. To clarify the function of this protein, we have undertaken a genetic analy...


    pub_type: 杂志文章


    authors: DiAntonio A,Parfitt KD,Schwarz TL

    更新日期:1993-07-02 00:00:00

  • Regulation of DLG localization at synapses by CaMKII-dependent phosphorylation.

    abstract::Discs large (DLG) mediates the clustering of synaptic molecules. Here we demonstrate that synaptic localization of DLG itself is regulated by CaMKII. We show that DLG and CaMKII colocalize at synapses and exist in the same protein complex. Constitutively activated CaMKII phenocopied structural abnormalities of dlg mut...


    pub_type: 杂志文章


    authors: Koh YH,Popova E,Thomas U,Griffith LC,Budnik V

    更新日期:1999-08-06 00:00:00

  • Nerve growth factor is an autocrine survival factor for memory B lymphocytes.

    abstract::Production of nerve growth factor (NGF) was assessed in cultures of human T and B lymphocytes and macrophages. NGF was constitutively produced by B cells only, which also expressed surface p140trk-A and p75NGFR molecules and hence efficiently bound and internalized the cytokine. Neutralization of endogenous NGF caused...


    pub_type: 杂志文章


    authors: Torcia M,Bracci-Laudiero L,Lucibello M,Nencioni L,Labardi D,Rubartelli A,Cozzolino F,Aloe L,Garaci E

    更新日期:1996-05-03 00:00:00

  • Genetic changes in skin tumor progression: correlation between presence of a mutant ras gene and loss of heterozygosity on mouse chromosome 7.

    abstract::Initiation of tumorigenesis in mouse skin can be accomplished by mutagenesis of the H-ras gene by treatment with chemical carcinogens. A mouse model system has been developed to study the additional genetic events that take place during tumor progression. Skin carcinomas were induced in F1 hybrid mice exhibiting restr...


    pub_type: 杂志文章


    authors: Bremner R,Balmain A

    更新日期:1990-05-04 00:00:00

  • Rosalind Franklin and the Advent of Molecular Biology.

    abstract::Rosalind Franklin provided the key data for deriving the double helix structure of DNA. The English chemist also pioneered structural studies of colloids, viruses, and RNA. To celebrate the 100th anniversary of Franklin's birth, I summarize her work, which shaped the emerging discipline of molecular biology. ...


    pub_type: 杂志文章


    authors: Cramer P

    更新日期:2020-08-20 00:00:00

  • Histone Modifications: Insights into Their Influence on Gene Expression.

    abstract::This year's Albert Lasker Basic Medical Research Award honors David Allis and Michael Grunstein for their pioneering research that highlighted the importance of histones and their post-translational modifications in the direct control of gene expression. ...


    pub_type: 传,历史文章,新闻


    authors: Stillman B

    更新日期:2018-09-20 00:00:00

  • Phosphodiesterase 4D deficiency in the ryanodine-receptor complex promotes heart failure and arrhythmias.

    abstract::Phosphodiesterases (PDEs) regulate the local concentration of 3',5' cyclic adenosine monophosphate (cAMP) within cells. cAMP activates the cAMP-dependent protein kinase (PKA). In patients, PDE inhibitors have been linked to heart failure and cardiac arrhythmias, although the mechanisms are not understood. We show that...


    pub_type: 杂志文章


    authors: Lehnart SE,Wehrens XH,Reiken S,Warrier S,Belevych AE,Harvey RD,Richter W,Jin SL,Conti M,Marks AR

    更新日期:2005-10-07 00:00:00

  • DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression.

    abstract::DMC1 is a new meiosis-specific yeast gene. Dmc1 protein is structurally similar to bacterial RecA proteins. dmc1 mutants are defective in reciprocal recombination, accumulate double-strand break (DSB) recombination intermediates, fail to form normal synaptonemal complex (SC), and arrest late in meiotic prophase. dmc1 ...


    pub_type: 杂志文章


    authors: Bishop DK,Park D,Xu L,Kleckner N

    更新日期:1992-05-01 00:00:00

  • p25rum1 orders S phase and mitosis by acting as an inhibitor of the p34cdc2 mitotic kinase.

    abstract::p25rum1 from the fission yeast S. pombe is shown to act as a specific in vitro inhibitor of the p34cdc2/p56cdc13 mitotic kinase. It is also shown that early G1 cells contain p25rum1, which associates with and inhibits the mitotic kinase, and maintains p56cdc13 mitotic B cyclin at a low level, ensuring that these cells...


    pub_type: 杂志文章


    authors: Correa-Bordes J,Nurse P

    更新日期:1995-12-15 00:00:00

  • Human l1 retrotransposition is associated with genetic instability in vivo.

    abstract::Retrotransposons have shaped eukaryotic genomes for millions of years. To analyze the consequences of human L1 retrotransposition, we developed a genetic system to recover many new L1 insertions in somatic cells. Forty-two de novo integrants were recovered that faithfully mimic many aspects of L1s that accumulated sin...


    pub_type: 杂志文章


    authors: Symer DE,Connelly C,Szak ST,Caputo EM,Cost GJ,Parmigiani G,Boeke JD

    更新日期:2002-08-09 00:00:00

  • Regulation of TORC1 in response to amino acid starvation via lysosomal recruitment of TSC2.

    abstract::TOR complex 1 (TORC1) is a potent anabolic regulator of cellular growth and metabolism. When cells have sufficient amino acids, TORC1 is active due to its lysosomal localization mediated via the Rag GTPases. Upon amino acid removal, the Rag GTPases release TORC1, causing it to become cytoplasmic and inactive. We show ...


    pub_type: 杂志文章


    authors: Demetriades C,Doumpas N,Teleman AA

    更新日期:2014-02-13 00:00:00

  • Six-microns-under acts upstream of Draper in the glial phagocytosis of apoptotic neurons.

    abstract::The removal of apoptotic cells by phagocytic neighbors is essential for metazoan development but remains poorly characterized. Here we report the discovery of a Drosophila phagocytosis receptor, Six-microns-under (SIMU), which is expressed in highly phagocytic cell types during development and required for efficient a...


    pub_type: 杂志文章


    authors: Kurant E,Axelrod S,Leaman D,Gaul U

    更新日期:2008-05-02 00:00:00

  • New surface component of fibroblast's focal contacts identified by a monoclonal antibody.

    abstract::We describe a monoclonal antibody (anti-FC-1) that inhibits the attachment of chicken embryo fibroblasts to artificial substrates. In the immunofluorescence, anti-FC-1 also stained the focal contacts of the fibroblasts, organelles suggested to be involved in cell-substrate adhesion. This antibody was absorbed on the s...


    pub_type: 杂志文章


    authors: Oesch B,Birchmeier W

    更新日期:1982-12-01 00:00:00

  • A Genomic Perspective on the Origin and Emergence of SARS-CoV-2.

    abstract::The ongoing pandemic of a new human coronavirus, SARS-CoV-2, has generated enormous global concern. We and others in China were involved in the initial genome sequencing of the virus. Herein, we describe what genomic data reveal about the emergence SARS-CoV-2 and discuss the gaps in our understanding of its origins. ...


    pub_type: 杂志文章


    authors: Zhang YZ,Holmes EC

    更新日期:2020-04-16 00:00:00

  • Fusion of the Escherichia coli tRNALeu1 promoter to the galK gene: analysis of sequences necessary for growth-rate-dependent regulation.

    abstract::We have fused DNA fragments derived from an Escherichia coli tRNALeu1 operon to the galK gene of E. coli to identify sequences necessary for the in vivo initiation of transcription and growth-rate-dependent regulation. Promoter sequences consisting of residues from -50 to +56 or -50 to +5 with respect to the in vivo s...


    pub_type: 杂志文章


    authors: Duester G,Elford RM,Holmes WM

    更新日期:1982-10-01 00:00:00

  • Analysis of a spatially regulated phosphotyrosine phosphatase identifies tyrosine phosphorylation as a key regulatory pathway in Dictyostelium.

    abstract::We have cloned a Dictyostelium phosphotyrosine phosphatase (PTP1) with a catalytic domain showing approximately 38%-50% amino acid identity to those of other PTPs. PTP1 contains an approximately 99 amino acid insert and bacterially produced PTP1 possesses PTP activity. PTP1 is expressed at a very low level in vegetati...


    pub_type: 杂志文章


    authors: Howard PK,Sefton BM,Firtel RA

    更新日期:1992-11-13 00:00:00

  • Chromosome missegregation and apoptosis in mice lacking the mitotic checkpoint protein Mad2.

    abstract::The initiation of chromosome segregation at anaphase is linked by the spindle assembly checkpoint to the completion of chromosome-microtubule attachment during metaphase. To determine the function of the mitotic checkpoint protein Mad2 during normal cell division and when mitosis goes awry, we have knocked out Mad2 in...


    pub_type: 杂志文章


    authors: Dobles M,Liberal V,Scott ML,Benezra R,Sorger PK

    更新日期:2000-06-09 00:00:00

  • A Common Embryonic Origin of Stem Cells Drives Developmental and Adult Neurogenesis.

    abstract::New neurons arise from quiescent adult neural progenitors throughout life in specific regions of the mammalian brain. Little is known about the embryonic origin and establishment of adult neural progenitors. Here, we show that Hopx+ precursors in the mouse dentate neuroepithelium at embryonic day 11.5 give rise to pro...


    pub_type: 杂志文章


    authors: Berg DA,Su Y,Jimenez-Cyrus D,Patel A,Huang N,Morizet D,Lee S,Shah R,Ringeling FR,Jain R,Epstein JA,Wu QF,Canzar S,Ming GL,Song H,Bond AM

    更新日期:2019-04-18 00:00:00

  • Ubiquitin is attached to membranes of baculovirus particles by a novel type of phospholipid anchor.

    abstract::Purified budded virions of Autographa californica nuclear polyhedrosis virus (AcNPV) contain abundant amounts of free ubiquitin, which has an altered electrophoretic mobility on SDS gels as compared with standard ubiquitin. Phase extraction of virion proteins with Triton X-114 indicated that the modified form of ubiqu...


    pub_type: 杂志文章


    authors: Guarino LA,Smith G,Dong W

    更新日期:1995-01-27 00:00:00

  • Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.

    abstract::Peptide growth factors may play a role in patterning of the early embryo, particularly in the induction of mesoderm. We have explored the role of fibroblast growth factor (FGF) in early Xenopus development by expressing a dominant negative mutant form of the FGF receptor. Using a functional assay in frog oocytes, we f...


    pub_type: 杂志文章


    authors: Amaya E,Musci TJ,Kirschner MW

    更新日期:1991-07-26 00:00:00

  • HMG CoA reductase: a negatively regulated gene with unusual promoter and 5' untranslated regions.

    abstract::The rate-limiting enzyme of cholesterol biosynthesis, HMG CoA reductase, is controlled by negative feedback regulation of transcription. We have isolated the reductase gene from a bacteriophage lambda genomic library prepared from hamster UT-1 cells. The 25 kilobase gene is split into 20 exons. The 5' untranslated and...


    pub_type: 杂志文章


    authors: Reynolds GA,Basu SK,Osborne TF,Chin DJ,Gil G,Brown MS,Goldstein JL,Luskey KL

    更新日期:1984-08-01 00:00:00

  • The existence of an insoluble Z disc scaffold in chicken skeletal muscle.

    abstract::Extraction of glycerinated chicken skeletal muscle with 0.6 M potassium iodide leaves a framework of insoluble components within each muscle fiber. This framework is composed primarily of planes of in-register Z discs that have been thickened by the accumulation of material on both sides of each disc during extraction...


    pub_type: 杂志文章


    authors: Granger BL,Lazarides E

    更新日期:1978-12-01 00:00:00

  • Prion-like Domains Program Ewing's Sarcoma.

    abstract::Prion-like domains have emerged as important drivers of neurodegenerative disease. Now, Boulay et al. establish that the translocated prion-like domain of the oncogenic EWS-FLI1 fusion protein enables phase-separation events, which inappropriately recruit chromatin-remodeling factors to elicit the aberrant transcripti...


    pub_type: 评论,杂志文章


    authors: Shorter J

    更新日期:2017-09-21 00:00:00