Molecular cloning and sequence analysis of a haploid expressed gene encoding t complex polypeptide 1.

Abstract:

:The mouse t haplotypes show defects in spermatogenesis attributed to multiple loci on chromosome 17. We have cloned the gene for an abundant testicular germ cell protein, t complex polypeptide 1, which has a variant form in t haplotypes, TCP-1A. A cDNA clone, pB1.4, which hybridizes to a 19S mRNA that is abundant in haploid cells during mouse spermatogenesis, derives from the 3' end of the mRNA encoding TCP-1B. The Tcp-1 gene appears to be a member of a novel gene family and shows multiple changes between the predicted amino acid sequences of TCP-1B and TCP-1A. An additional Taq1 site is created by a T to C transition in the predicted open reading frame of the Tcp-1a gene. The resultant RFLP has allowed typing of the Tcp-1 gene cluster in 54 complete and partial t haplotype chromosomes. DNA sequence comparison of the Tcp-1 genes suggests that the t haplotype chromosome arose within the genus Mus more than one million years ago.

journal_name

Cell

journal_title

Cell

authors

Willison KR,Dudley K,Potter J

doi

10.1016/0092-8674(86)90839-1

subject

Has Abstract

pub_date

1986-03-14 00:00:00

pages

727-38

issue

5

eissn

0092-8674

issn

1097-4172

pii

0092-8674(86)90839-1

journal_volume

44

pub_type

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