Abstract：
:The SIR2, SIR3, and SIR4 proteins are required for silencing of transcription at the silent mating type loci and at telomeres in yeast. Using protein affinity chromatography, we show that SIR2, SIR3, and two proteins of 69 and 110 kDa tightly associate with SIR4. Surprisingly, the 110 kDa SIR4-binding protein is identical to UBP3, one of several previously described yeast enzymes that deubiquitinate target proteins. Deletion of the UBP3 gene results in markedly improved silencing of genes inserted either near a telomere or at one of the silent mating type loci, indicating that UBP3 is an inhibitor of silencing. We discuss possible roles for UBP3 in controlling the activity or assembly of the SIR protein complex.
journal_name
Celljournal_title
Cellauthors
Moazed D,Johnson Ddoi
10.1016/s0092-8674(00)80139-7subject
Has Abstractpub_date
1996-08-23 00:00:00pages
667-77issue
4eissn
0092-8674issn
1097-4172pii
S0092-8674(00)80139-7journal_volume
86pub_type
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