Abstract:
:A study in this issue of Cell illustrates the power of applying genomic approaches with model systems to characterize the biological activity of small molecules and to identify their cellular targets, which can clarify the mode of action of human therapeutics.
journal_name
Celljournal_title
Cellauthors
Hughes T,Andrews B,Boone Cdoi
10.1016/s0092-8674(03)01070-5subject
Has Abstractpub_date
2004-01-09 00:00:00pages
5-7issue
1eissn
0092-8674issn
1097-4172pii
S0092867403010705journal_volume
116pub_type
评论,杂志文章,评审相关文献
CELL文献大全abstract::Synaptic plasticity in response to changes in physiologic state is coordinated by hormonal signals across multiple neuronal cell types. Here, we combine cell-type-specific electrophysiological, pharmacological, and optogenetic techniques to dissect neural circuits and molecular pathways controlling synaptic plasticity...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.07.039
更新日期:2011-09-16 00:00:00
abstract::The asymmetries of internal organs are consistently oriented along the left-right axis in all vertebrates, and perturbations of left-right orientation lead to significant congenital disease. We propose a model in which a "left-right coordinator" interacts with the Spemann organizer to coordinate the evolutionarily con...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81144-7
更新日期:1998-04-03 00:00:00
abstract::We studied the chromatin structure and methylation of ribosomal RNA genes (rDNA) in hybrids between Xenopus laevis and Xenopus borealis. S1-nuclease protection experiments showed that 97%-98% of the rRNA precursor in hybrid tadpoles was of the X. laevis type. Preferential expression of the laevis rDNA was correlated w...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90105-2
更新日期:1982-05-01 00:00:00
abstract::DNA sequence analysis of a class I gene (Q10), which maps to the Qa2,3 locus in the C57BL/10 (H-2b haplotype) mouse, reveals that it is almost identical to a cDNA clone (pH16) isolated from a SWR/J (H-2q haplotype) mouse liver cDNA library. Exon 5, in particular, has an unusual structure such that a polypeptide produc...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90082-5
更新日期:1984-01-01 00:00:00
abstract::ABCA1, an ATP-binding cassette (ABC) subfamily A exporter, mediates the cellular efflux of phospholipids and cholesterol to the extracellular acceptor apolipoprotein A-I (apoA-I) for generation of nascent high-density lipoprotein (HDL). Mutations of human ABCA1 are associated with Tangier disease and familial HDL defi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.05.020
更新日期:2017-06-15 00:00:00
abstract::Cellular functioning requires the orchestration of thousands of molecular interactions in time and space. Yet most molecules in a cell move by diffusion, which is sensitive to external factors like temperature. How cells sustain complex, diffusion-based systems across wide temperature ranges is unknown. Here, we uncov...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.10.017
更新日期:2020-12-10 00:00:00
abstract::The low-complexity (LC) domains of the products of the fused in sarcoma (FUS), Ewings sarcoma (EWS), and TAF15 genes are translocated onto a variety of different DNA-binding domains and thereby assist in driving the formation of cancerous cells. In the context of the translocated fusion proteins, these LC sequences fu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.10.033
更新日期:2013-11-21 00:00:00
abstract::Most of the Dictyostelium ribosomal nontranscribed spacer DNA has been mapped using Eco R1, Hind III, and Sal I restriction digests and recombinant plasmids containing parts of the rDNA (ribosomal DNA). The repeat units are at least 42 kb (1 kilobase = 1000 base pairs = 1 kb) long; of this, 8 kb codes for the 36S rRNA...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(76)90043-x
更新日期:1976-12-01 00:00:00
abstract::When treated with the synthetic glucocorticoid dexamethasone, HT1080 human fibrosarcoma cells show changes in morphology, adhesion, and the extracellular matrix. Dexamethasone treatment results in a tenfold increase in the rate of fibronectin biosynthesis in HT1080 cells and a twofold increase in untransformed, normal...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90357-4
更新日期:1983-05-01 00:00:00
abstract::Activation of Agouti-related peptide (AgRP) neurons potently promotes feeding, and chronically altering their activity also affects peripheral glucose homeostasis. We demonstrate that acute activation of AgRP neurons causes insulin resistance through impairment of insulin-stimulated glucose uptake into brown adipose t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.02.044
更新日期:2016-03-24 00:00:00
abstract::Periodic transcription of yeast histone genes has been demonstrated by DNA excess filter hybridization of in vivo pulse-labeled RNA isolated from synchronous cell cultures. Using strains carrying cell division cycle (cdc) mutations, we show that both activation and termination of transcription are determined by tempor...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90036-8
更新日期:1982-08-01 00:00:00
abstract::The nucleotide sequence from the 5' terminus inward of one third of mouse alpha- and beta maj-globin messenger RNAs has been established. In addition, using 5' 32P end-labeled mRNAs as substrates and S1 and T1 nucleases as probes for single-stranded regions, the secondary structures of mouse and rabbit alpha- and beta...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90391-8
更新日期:1980-01-01 00:00:00
abstract::Mutations in four nuclear genes, kar1 cdc4, 28, and 37, block or impair nuclear fusion during conjugation of Saccharomyces cerevisiae. Mutations in all four genes are recessive for the caryogamy defect; in matings between diploid cells both of which are heterozygous for any one of the four mutations (-/+ X -/+), caryo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90349-5
更新日期:1983-05-01 00:00:00
abstract::Gastrointestinal enterochromaffin cells regulate bone and gut homeostasis via serotonin (5-hydroxytryptamine [5-HT]) production. A recent report suggested that gut microbes regulate 5-HT levels; however, the precise underlying molecular mechanisms are unexplored. Here, we reveal that the cation channel Piezo1 in the g...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.06.022
更新日期:2020-08-06 00:00:00
abstract::The origin recognition complex (ORC) is required to initiate eukaryotic DNA replication and also engages in transcriptional silencing in S. cerevisiae. We observed a striking preferential but not exclusive association of Drosophila ORC2 with heterochromatin on interphase and mitotic chromosomes. HP1, a heterochromatin...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80415-8
更新日期:1997-10-31 00:00:00
abstract::Tumor suppressors block the development of cancer and are often lost during tumor development. Papa et al. show that partial loss of normal PTEN tumor suppressor function can be compounded by additional disruption caused by the expression of inactive mutant PTEN protein. This has significant implications for patients ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2014.04.008
更新日期:2014-04-24 00:00:00
abstract::Exposure to Mycobacterium tuberculosis produces varied early outcomes, ranging from resistance to infection to progressive disease. Here we report results from a forward genetic screen in zebrafish larvae that identify multiple mutant classes with distinct patterns of innate susceptibility to Mycobacterium marinum. A ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2010.02.013
更新日期:2010-03-05 00:00:00
abstract::Elements controlling tissue-specific expression of the rat albumin gene reside within roughly 150 bp upstream of the transcriptional initiation site. We show here by DNAase I footprinting assays that at least four distinct factors present in extracts derived from cells expressing albumin interact with these sequences....
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90036-5
更新日期:1987-08-14 00:00:00
abstract::Following DNA replication, eukaryotic cells must biorient all sister chromatids prior to cohesion cleavage at anaphase. In animal cells, sister chromatids gradually biorient during prometaphase, but current models of mitosis in S. cerevisiae assume that biorientation is established shortly after S phase. This assumpti...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.08.008
更新日期:2013-08-29 00:00:00
abstract::Drosophila Reaper (RPR), Head Involution Defective (HID), and GRIM induce caspase-dependent cell death and physically interact with the cell death inhibitor DIAP1. Here we show that HID blocks DIAP1's ability to inhibit caspase activity and provide evidence suggesting that RPR and GRIM can act similarly. Based on thes...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81974-1
更新日期:1999-08-20 00:00:00
abstract::We describe here a bizarre organization of the single large subunit (LSU) and small subunit (SSU) rRNA genes in the mitochondrial DNA of Chlamydomonas reinhardtii. Each gene is discontinuous, with gene pieces encoding specific rRNA domains ("modules") interspersed with one another and with intact protein coding and tR...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(88)90026-8
更新日期:1988-11-04 00:00:00
abstract::Generation of potent antibodies by a mutation-selection process called affinity maturation is a key component of effective immune responses. Antibodies that protect against highly mutable pathogens must neutralize diverse strains. Developing effective immunization strategies to drive their evolution requires understan...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.01.027
更新日期:2015-02-12 00:00:00
abstract::The PML tumor suppressor controls key pathways for growth suppression, induction of apoptosis, and cellular senescence. PML loss occurs frequently in human tumors through unknown posttranslational mechanisms. Casein kinase 2 (CK2) is oncogenic and frequently upregulated in human tumors. Here we show that CK2 regulates...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.05.041
更新日期:2006-07-28 00:00:00
abstract::torso encodes a receptor tyrosine kinase (torso) required for anterior and posterior terminal development of the Drosophila embryo. Injecting eggs with in vitro synthesized torso mRNAs revealed that torso activation is governed by an extracellular molecule, presumably the torso ligand, produced at terminal regions of ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90394-r
更新日期:1992-12-11 00:00:00
abstract::The maternal Dorsal regulatory gradient initiates the differentiation of several tissues in the early Drosophila embryo. Whole-genome microarray assays identified as many as 40 new Dorsal target genes, which encode a broad spectrum of cell signaling proteins and transcription factors. Evidence is presented that a tiss...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(02)01087-5
更新日期:2002-11-27 00:00:00
abstract::We have identified a gene, SRV2, mutations of which alleviate stress sensitivity in strains carrying an activated RAS gene. Epistasis analysis suggests that the gene affects accumulation of cAMP in the cell. Direct assays of cAMP accumulation indicate that mutations of the gene diminish the rate of in vivo production ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90813-t
更新日期:1990-04-20 00:00:00
abstract::Twitter needs no introduction. This "microblogging" service has gained tremendous popularity in the 2 years since its launch. Yet, most scientists are steering clear of it. Laura Bonetta speaks to some who have found value in tweeting. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.10.017
更新日期:2009-10-30 00:00:00
abstract::Mouse macrophages and lymphocytes express two distinct isoforms of a single class of Fc receptor for IgG. The macrophage isoform (FcRII-B2) is identical to the lymphocyte isoform (FcRII-B1) except for an inframe insertion in the cytoplasmic tail of FcRII-B1 that increases its length from 47 to 94 amino acids. To deter...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90846-5
更新日期:1989-07-28 00:00:00
abstract::In embryonal carcinoma (EC) cells retinoic acid (RA) strongly induces transcription from the RA receptor beta 2 (RAR beta 2) promoter through an RA response element (RARE) located in close proximity to the TATA box. Here we demonstrate that recombinant human TATA box-binding protein, hTFIID, and RAR functionally coope...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90443-g
更新日期:1992-05-01 00:00:00
abstract::An antiserum against material shed into serum-free medium by MCF-7 human mammary carcinoma cells (anti-SFM II) disrupts cell-cell interactions in murine mammary tumor epithelial cells (MMTE). We now report purification of an 80 kd glycoprotein (GP80) from SFM of MCF-7 mammary carcinoma cells that blocks the activity o...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90379-3
更新日期:1983-09-01 00:00:00