Abstract:
:Regulated transcription of the prospero gene in the Drosophila eye provides a model for how gene expression is specifically controlled by signals from receptor tyrosine kinases. We show that prospero is controlled by signals from the EGF receptor DER and the Sevenless receptor. A direct link is established between DER activation of a transcription enhancer in prospero and binding of two transcription factors that are targets of DER signaling. Binding of the cell-specific Lozenge protein is also required for activation, and overlapping Lozenge protein distribution and DER signaling establishes expression in a subset of equivalent cells competent to respond to Sevenless. We show that Sevenless activates prospero independent of the enhancer and involves targeted degradation of Tramtrack, a transcription repressor.
journal_name
Celljournal_title
Cellauthors
Xu C,Kauffmann RC,Zhang J,Kladny S,Carthew RWdoi
10.1016/s0092-8674(00)00107-0subject
Has Abstractpub_date
2000-09-29 00:00:00pages
87-97issue
1eissn
0092-8674issn
1097-4172pii
S0092-8674(00)00107-0journal_volume
103pub_type
杂志文章相关文献
CELL文献大全abstract::Rapid flow of information in the nervous system involves presynaptic vesicle recycling by clathrin-mediated endocytosis, an event triggered by the alpha-adaptin-containing AP2 complex. We identified a Drosophila alpha-adaptin expressed in the garland cells, imaginal discs, and the CNS. Here we show its role in presyna...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81923-6
更新日期:1997-03-21 00:00:00
abstract::Induction of pluripotency in somatic cells has been achieved by myriad combinations of transcription factors that belong to the core pluripotency circuitry. In this issue, Shu et al. report reprogramming with lineage specifiers, lending support to the view of the pluripotent state as a fine balance between competing d...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2013.04.051
更新日期:2013-05-23 00:00:00
abstract::The gut epithelium self-renews every several days, providing an important innate defense system that limits bacterial colonization. Nevertheless, many bacterial pathogens, including Shigella, efficiently colonize the intestinal epithelium. Here, we show that the Shigella effector IpaB, when delivered into epithelial c...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.06.043
更新日期:2007-08-24 00:00:00
abstract::We have isolated and characterized a set of overlapping cDNA clones that encode the human centromere autoantigen centromere protein C (CENP-C). The identity of these clones has been established using several criteria. First, they were shown to encode a polypeptide that migrates at the expected position for CENP-C on S...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90538-n
更新日期:1992-07-10 00:00:00
abstract::Constitutive egress of bone marrow (BM)-resident hematopoietic stem and progenitor cells (HSPCs) into the blood is a well-established phenomenon, but the ultimate fate and functional relevance of circulating HSPCs is largely unknown. We show that mouse thoracic duct (TD) lymph contains HSPCs that possess short- and lo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.09.047
更新日期:2007-11-30 00:00:00
abstract::Tyrosyl phosphorylation is implicated in the mechanism of insulin action. Mutation of the beta-subunit of the insulin receptor by substitution of tyrosyl residue 960 with phenylalanine had no effect on insulin-stimulated autophosphorylation or phosphotransferase activity of the purified receptor. However, unlike the n...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(88)80008-4
更新日期:1988-08-26 00:00:00
abstract::Kinetochores connect centromeric nucleosomes with mitotic-spindle microtubules through conserved, cross-interacting protein subassemblies. In budding yeast, the heterotetrameric MIND complex (Mtw1, Nnf1, Nsl1, Dsn1), ortholog of the metazoan Mis12 complex, joins the centromere-proximal components, Mif2 and COMA, with ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.10.011
更新日期:2016-11-03 00:00:00
abstract::Monkey cells synthesizing SV40 large T antigen were lysed and the extracts immunoprecipitated with either monoclonal anti-T antibody or monoclonal antibody to p110-114, the product of the retinoblastoma susceptibility gene (Rb). T and p110-114 coprecipitated in each case, implying that the proteins are complexed with ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(88)90559-4
更新日期:1988-07-15 00:00:00
abstract::247 independent events involving insertion of the TN3 transposable element into a 4 kb constructed plasmid (pTU4) of partially known DNA sequence were studied by restriction endonuclease mapping, and 65 of these insertion sites were examined further by DNA sequence analysis. Our results show that the previously propos...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90396-7
更新日期:1980-01-01 00:00:00
abstract::Complex multicellular organisms, such as mammals, express two complete sets of chromosomes per nucleus, combining the genetic material of both parents. However, epigenetic studies have demonstrated violations to this rule that are necessary for mammalian physiology; the most notable parental allele expression phenomen...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2019.01.043
更新日期:2019-02-21 00:00:00
abstract::We have identified a gene, SRV2, mutations of which alleviate stress sensitivity in strains carrying an activated RAS gene. Epistasis analysis suggests that the gene affects accumulation of cAMP in the cell. Direct assays of cAMP accumulation indicate that mutations of the gene diminish the rate of in vivo production ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90813-t
更新日期:1990-04-20 00:00:00
abstract::We examine the interaction between polyoma-virus-encoded middle tumor antigen and the cellular src gene product, pp60c-src, using a series of monoclonal antibodies that recognize mammalian pp60c-src. Our results show that infection of mouse cells with transformation-competent strains of polyoma virus results in the st...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90272-1
更新日期:1984-10-01 00:00:00
abstract::Elucidation of endogenous cellular protein-protein interactions and their networks is most desirable for biological studies. Here we report our study of endogenous human coregulator protein complex networks obtained from integrative mass spectrometry-based analysis of 3290 affinity purifications. By preserving weak pr...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.05.006
更新日期:2011-05-27 00:00:00
abstract::The sites at which topoisomerase I interacts with the transcriptionally active ribosomal chromatin of Xenopus oocytes were mapped by treating oocyte nuclei first with camptothecin to stabilize topoisomerase I-rDNA reaction intermediates, and then with SDS to resolve these adducts as protein-linked nicks in the DNA. St...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(88)90471-0
更新日期:1988-02-26 00:00:00
abstract::The Clock mutation lengthens periodicity and reduces amplitude of circadian rhythms in mice. The effects of Clock are cell intrinsic and can be observed at the level of single neurons in the suprachiasmatic nucleus. To address how cells of contrasting genotype functionally interact in vivo to control circadian behavio...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00294-x
更新日期:2001-04-06 00:00:00
abstract::The transmembrane ligand ephrinB2 and its cognate Eph receptor tyrosine kinases are important regulators of vascular morphogenesis. EphrinB2 may have an active signaling role, resulting in bi-directional signal transduction downstream of both ephrinB2 and Eph receptors. To separate the ligand and receptor-like functio...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00191-x
更新日期:2001-01-12 00:00:00
abstract::Development of microbiota-directed foods (MDFs) that selectively increase the abundance of beneficial human gut microbes, and their expressed functions, requires knowledge of both the bioactive components of MDFs and the mechanisms underlying microbe-microbe interactions. Here, gnotobiotic mice were colonized with a d...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.08.011
更新日期:2019-09-19 00:00:00
abstract::Brown and beige adipocytes are specialized cells that express uncoupling protein 1 (UCP1) and dissipate chemical energy as heat. These cells likely possess alternative UCP1-independent thermogenic mechanisms. Here, we identify a secreted enzyme, peptidase M20 domain containing 1 (PM20D1), that is enriched in UCP1(+) v...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.05.071
更新日期:2016-07-14 00:00:00
abstract::Inward rectifier K+ channels mediate the K+ conductance at resting potential in many types of cell. Since these K+ channels do not pass outward currents (inward rectification) when the cell membrane is depolarized beyond a trigger threshold, they play an important role in controlling excitability. Both a highly voltag...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90459-x
更新日期:1995-01-13 00:00:00
abstract::Sexual reproduction is almost universal in eukaryotic life and involves the fusion of male and female haploid gametes into a diploid cell. The sperm-restricted single-pass transmembrane protein HAP2-GCS1 has been postulated to function in membrane merger. Its presence in the major eukaryotic taxa-animals, plants, and ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.01.024
更新日期:2017-02-23 00:00:00
abstract::Gold conjugates have been used to quantitate human transferrin receptors (hTfnRs) on transfected chick embryo fibroblasts. No relationship could be found between the number of hTfnRs and the number of clathrin-coated pits. However, hTfnRs are also associated with flat clathrin lattices that lie outside invaginated pit...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90094-f
更新日期:1991-05-17 00:00:00
abstract::p25rum1 from the fission yeast S. pombe is shown to act as a specific in vitro inhibitor of the p34cdc2/p56cdc13 mitotic kinase. It is also shown that early G1 cells contain p25rum1, which associates with and inhibits the mitotic kinase, and maintains p56cdc13 mitotic B cyclin at a low level, ensuring that these cells...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90215-5
更新日期:1995-12-15 00:00:00
abstract::Here we characterize the T-cell-activating protein (TAP), an Ly-6 gene product involved in T cell activation, as a glycoprotein with a molecular weight of 10-12 kd under nonreducing conditions and 15-18 kd under reducing ones. Two of the three bands that are precipitated from metabolically labeled cells are expressed ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90593-3
更新日期:1986-11-07 00:00:00
abstract::Increased protein synthesis plays an etiologic role in diverse cancers. Here, we demonstrate that METTL13 (methyltransferase-like 13) dimethylation of eEF1A (eukaryotic elongation factor 1A) lysine 55 (eEF1AK55me2) is utilized by Ras-driven cancers to increase translational output and promote tumorigenesis in vivo. ME...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.11.038
更新日期:2019-01-24 00:00:00
abstract::Hybrid transducing phages were constructed in vitro that carry the galK gene fused to each of three ribosomal promoters: the promotor for an rRNA operon (rrnE); the promoter for the spec r protein operon and the promotor for the alpha r protein operon. We also constructed hybrid transducing phages that carry the IacZ ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90185-9
更新日期:1981-09-01 00:00:00
abstract::Genetics and pharmacology can elicit surprisingly different phenotypes despite targeting the same protein. This Essay explores these unexpected differences and their implications for biology and medicine. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.01.021
更新日期:2007-02-09 00:00:00
abstract::The chaperonin GroEL binds nonnative proteins too large to fit inside the productive GroEL-GroES cis cavity, but whether and how it assists their folding has remained unanswered. We have examined yeast mitochondrial aconitase, an 82 kDa monomeric Fe(4)S(4) cluster-containing enzyme, observed to aggregate in chaperonin...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00523-2
更新日期:2001-10-19 00:00:00
abstract::DNA transposition has contributed significantly to evolution of eukaryotes and prokaryotes. Insertion sequences (ISs) are the simplest prokaryotic transposons and are divided into families on the basis of their organization and transposition mechanism. Here, we describe a link between transposition of IS608 and ISDra2...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2010.06.034
更新日期:2010-08-06 00:00:00
abstract::X inactivation results in inactivation of one X chromosome to compensate for gene dosage differences between mammalian females and males. It requires the X-inactivation center (Xic) and Xist in cis. We report that introducing 450 kb of murine Xic/Xist sequences onto autosomes activates female dosage compensation in ma...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80079-3
更新日期:1996-07-12 00:00:00
abstract::Spindle assembly requires the coordinated action of multiple cellular structures to nucleate and organize microtubules in a precise spatiotemporal manner. Among them, the contributions of centrosomes, chromosomes, and microtubules have been well studied, yet the involvement of membrane-bound organelles remains largely...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.06.014
更新日期:2015-07-16 00:00:00