Abstract:
:Spindle assembly requires the coordinated action of multiple cellular structures to nucleate and organize microtubules in a precise spatiotemporal manner. Among them, the contributions of centrosomes, chromosomes, and microtubules have been well studied, yet the involvement of membrane-bound organelles remains largely elusive. Here, we provide mechanistic evidence for a membrane-based, Golgi-derived microtubule assembly pathway in mitosis. Upon mitotic entry, the Golgi matrix protein GM130 interacts with importin α via a classical nuclear localization signal that recruits importin α to the Golgi membranes. Sequestration of importin α by GM130 liberates the spindle assembly factor TPX2, which activates Aurora-A kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GM130, thus linking Golgi membranes to the spindle. Our results reveal an active role for the Golgi in regulating spindle formation to ensure faithful organelle inheritance.
journal_name
Celljournal_title
Cellauthors
Wei JH,Zhang ZC,Wynn RM,Seemann Jdoi
10.1016/j.cell.2015.06.014subject
Has Abstractpub_date
2015-07-16 00:00:00pages
287-299issue
2eissn
0092-8674issn
1097-4172pii
S0092-8674(15)00697-2journal_volume
162pub_type
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