Abstract：
:The dual host range virus HIX has been previously characterized as an envelope gene recombinant between Moloney murine leukemia virus (Mo-MuLV) and an unidentified xenotropic murine leukemia virus. Using long reverse transcripts of Mo-MuLV, a region of nonhomology has been mapped by electron microscopic analysis of heteroduplexes formed with HIX 35S virion RNA. In this nonhomology region, the Mo-MuLV cDNA strand measured approximately 900 nucleotides, mapping between 1.6 and 2.5 kilobases from the 3' end. In a previous study, hybridization of Mo-MuLV 21S RNA with Mo-MuLV cDNA resulted in the formation of different heteroduplex structures diagnostic of a noncontiguously coded leader sequence at the 5' end of the 21S RNA. Following hybridization of poly(A)+ HIX 21S RAN with 8.2 kb Mo-MuLV cDNA, analogous heteroduplex structures were observed exhibiting the Mo-MuLV:HIX substitution loop in the DNA:RNA segment of the molecules. This analysis permitted more precise mapping of the nonhomology region with respect to the splice point in the 21S presumptive glycoprotein mRNA. The mapping of this nonhomology region in HIX virus provides an internal visual marker for the 3' end of the genome which may prove useful in future analyses of other deletion or substitution derivatives of Mo-MuLV.
journal_name
Celljournal_title
Cellauthors
Donoghue DJ,Rothenberg E,Hopkins N,Baltimore D,Sharp PAdoi
10.1016/0092-8674(78)90350-1subject
Has Abstractpub_date
1978-08-01 00:00:00pages
959-70issue
4eissn
0092-8674issn
1097-4172pii
0092-8674(78)90350-1journal_volume
14pub_type
杂志文章相关文献
CELL文献大全abstract：:This year's Albert Lasker Basic Medical Research Award honors David Allis and Michael Grunstein for their pioneering research that highlighted the importance of histones and their post-translational modifications in the direct control of gene expression. ...
journal_title：Cell
pub_type： 传,历史文章,新闻
doi：10.1016/j.cell.2018.08.032
更新日期：2018-09-20 00:00:00
abstract：:Mitochondrial DNA from bovine tissue contains heterogeneous sequences located within an evolutionary conserved cytosine homopolymer sequence near the 5' end of the D-loop region. This part of the mammalian mitochondrial genome is known to contain the origin of heavy strand DNA synthesis and the major transcriptional p...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(84)90434-3
更新日期：1984-07-01 00:00:00
abstract：:The transforming protein of Rous sarcoma virus (pp60v-src) and its normal cellular homolog (pp60c-src) are demonstrated to be phosphorylated at serine 12 in vivo under certain conditions. We propose that protein kinase C is responsible for this modification based on the following evidence. First, the tumor promoters, ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(85)90281-8
更新日期：1985-10-01 00:00:00
abstract：:Ebola virus (EBOV) remains a public health threat. We performed a longitudinal study of B cell responses to EBOV in four survivors of the 2014 West African outbreak. Infection induced lasting EBOV-specific immunoglobulin G (IgG) antibodies, but their subclass composition changed over time, with IgG1 persisting, IgG3 r...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.04.036
更新日期：2019-05-30 00:00:00
abstract：:We have identified a gene, SRV2, mutations of which alleviate stress sensitivity in strains carrying an activated RAS gene. Epistasis analysis suggests that the gene affects accumulation of cAMP in the cell. Direct assays of cAMP accumulation indicate that mutations of the gene diminish the rate of in vivo production ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90813-t
更新日期：1990-04-20 00:00:00
abstract：:Acquisition of the intestinal microbiota begins at birth, and a stable microbial community develops from a succession of key organisms. Disruption of the microbiota during maturation by low-dose antibiotic exposure can alter host metabolism and adiposity. We now show that low-dose penicillin (LDP), delivered from birt...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.05.052
更新日期：2014-08-14 00:00:00
abstract：:Conversion of glial cells into functional neurons represents a potential therapeutic approach for replenishing neuronal loss associated with neurodegenerative diseases and brain injury. Previous attempts in this area using expression of transcription factors were hindered by the low conversion efficiency and failure o...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.03.024
更新日期：2020-04-30 00:00:00
abstract：:Tumor suppressors block the development of cancer and are often lost during tumor development. Papa et al. show that partial loss of normal PTEN tumor suppressor function can be compounded by additional disruption caused by the expression of inactive mutant PTEN protein. This has significant implications for patients ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2014.04.008
更新日期：2014-04-24 00:00:00
abstract：:Neural inputs from internal organs are essential for normal autonomic function. The vagus nerve is a key body-brain connection that monitors the digestive, cardiovascular, and respiratory systems. Within the gastrointestinal tract, vagal sensory neurons detect gut hormones and organ distension. Here, we investigate th...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.05.011
更新日期：2016-06-30 00:00:00
abstract：:The assembly of COPI into a cage-like lattice sculpts membrane vesicles that transport cargo from the Golgi apparatus. Now, Lee and Goldberg (2010) present X-ray crystal structures of COPI suggesting that these coats combine selected features of two other archetypal coats, clathrin and COPII. ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2010.06.031
更新日期：2010-07-09 00:00:00
abstract：:CRISPR-Cas9 is a versatile genome editing technology for studying the functions of genetic elements. To broadly enable the application of Cas9 in vivo, we established a Cre-dependent Cas9 knockin mouse. We demonstrated in vivo as well as ex vivo genome editing using adeno-associated virus (AAV)-, lentivirus-, or parti...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.09.014
更新日期：2014-10-09 00:00:00
abstract：:A novel trimolecular mechanism of microbial attachment to mammalian host cells was characterized for the obligate intracellular pathogen Chlamydia trachomatis. Using purified glycosaminoglycans (GAGs) and specific GAG lyases, we demonstrated that a heparan sulfate-like GAG present on the surface of chlamydia organisms...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90296-o
更新日期：1992-05-29 00:00:00
abstract：:Replicative intermediates of SV40 were isolated, digested with the restriction endonuclease Bgl I and examined by electron microscopy. Over 98% of the replicative intermediates isolated following infection with wild-type virions at 33 degrees, 37 degrees or 40 degrees C or with tsA209 at 33 degrees C had initiated rep...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(80)90624-8
更新日期：1980-06-01 00:00:00
abstract：:A new rhythm mutation was isolated based on its elimination of per-controlled luciferase cycling. Levels of period or timeless clock gene products in the mutant are flat in daily light-dark cycles or constant darkness (although PER and TIM oscillate normally in temperature cycles). Consistent with the fact that light ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81638-4
更新日期：1998-11-25 00:00:00
abstract：:The plant innate immune response includes the hypersensitive response (HR), a form of programmed cell death (PCD). PCD must be restricted to infection sites to prevent the HR from playing a pathologic rather than protective role. Here we show that plant BECLIN 1, an ortholog of the yeast and mammalian autophagy gene A...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2005.03.007
更新日期：2005-05-20 00:00:00
abstract：:In a 4-cell stage C. elegans embryo, signaling by the P2 blastomere induces anterior-posterior polarity in the adjacent EMS blastomere, leading to endoderm formation. We have taken genetic and reverse genetic approaches toward understanding the molecular basis for this induction. These studies have identified a set of...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80531-0
更新日期：1997-08-22 00:00:00
abstract：:Chemotherapy results in a frequent yet poorly understood syndrome of long-term neurological deficits. Neural precursor cell dysfunction and white matter dysfunction are thought to contribute to this debilitating syndrome. Here, we demonstrate persistent depletion of oligodendrocyte lineage cells in humans who received...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.10.049
更新日期：2019-01-10 00:00:00
abstract：:To determine the function of c-fps in chicken macrophages and granulocytic cells we have infected chicken bone marrow cells with retroviruses containing the v-fps oncogene. Normal chicken macrophage progenitors, M-CFCs, give rise to macrophage colonies in semisolid cultures when macrophage colony stimulating factor (M...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(86)90494-0
更新日期：1986-01-17 00:00:00
abstract：:NAD(+) is an important cofactor regulating metabolic homeostasis and a rate-limiting substrate for sirtuin deacylases. We show that NAD(+) levels are reduced in aged mice and Caenorhabditis elegans and that decreasing NAD(+) levels results in a further reduction in worm lifespan. Conversely, genetic or pharmacological...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.06.016
更新日期：2013-07-18 00:00:00
abstract：:The haywire gene of Drosophila encodes a protein with 66% identity to the product of the human ERCC3 gene, associated with xeroderma pigmentosum B (XP-B) and Cockayne's syndrome (CS). XP is a human autosomal recessive disease characterized by extreme sensitivity to ultraviolet irradiation and marked susceptibility to ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90389-t
更新日期：1992-12-11 00:00:00
abstract：:A plasmid containing the major late promoter was assembled into nucleosomes in a Xenopus oocyte extract, isolated by gel filtration, and found to be refractory to transcription initiation in vitro. However, exposure of the promoter to HeLa nuclear extract or to a mixture of isolated transcription factors prior to nucl...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(87)90130-9
更新日期：1987-11-20 00:00:00
abstract：:The mechanisms by which molecular chaperones assist quality control of cytosolic proteins are poorly understood. Analysis of the chaperone requirements for degradation of misfolded variants of a cytosolic protein, the VHL tumor suppressor, reveals that distinct chaperone pathways mediate its folding and quality contro...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2005.03.024
更新日期：2005-06-03 00:00:00
abstract：:Rapid flow of information in the nervous system involves presynaptic vesicle recycling by clathrin-mediated endocytosis, an event triggered by the alpha-adaptin-containing AP2 complex. We identified a Drosophila alpha-adaptin expressed in the garland cells, imaginal discs, and the CNS. Here we show its role in presyna...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81923-6
更新日期：1997-03-21 00:00:00
abstract：:Clones containing five non-adult beta-globin genes were isolated from a library of BALB/c DNA. Together, the newly cloned regions comprise a contiguous block of 32 kb of the mouse genome. Restriction mapping of genomic DNA established the physical linkage of these non-adult genes to the two adult beta-globin genes as ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(80)90123-3
更新日期：1980-08-01 00:00:00
abstract：:In causing disease, pathogens outmaneuver host defenses through a dedicated arsenal of virulence determinants that specifically bind or modify individual host molecules. This dedication limits the intruder to a defined range of hosts. Newly emerging diseases mostly involve existing pathogens whose arsenal has been alt...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2007.03.049
更新日期：2007-06-01 00:00:00
abstract：:A recent comparative analysis of the sequenced genomes of 12 Drosophila species (Drosophila 12 Genomes Consortium, 2007; Stark et al., 2007) reveals a comprehensive picture of the evolution of small animal genomes and greatly improves computational predictions of functional elements in the D. melanogaster reference se...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2007.12.003
更新日期：2007-12-28 00:00:00
abstract：:P-selectin binding to neutrophils requires a specific protein, P-selectin glycoprotein ligand 1 (PSGL-1), as well as sialyl-Lewis X (sLex) glycan determinants. We have found that a short segment near the amino terminus of PSGL-1 that contains a tyrosine sulfation consensus is essential for P-selectin adhesion and that...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90174-4
更新日期：1995-10-20 00:00:00
abstract：:DNA demethylating agents are approved for some blood malignancies and are under active investigation in solid tumors, but how these drugs work has remained unclear. In this issue of Cell, two groups show that these agents activate a toxic cellular antiviral program through transcriptional activation of endogenous retr...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2015.08.005
更新日期：2015-08-27 00:00:00
abstract：:Eukaryotic cells sterilize the cytosol by using autophagy to route invading bacterial pathogens to the lysosome. During macrophage infection with Mycobacterium tuberculosis, a vacuolar pathogen, exogenous induction of autophagy can limit replication, but the mechanism of autophagy targeting and its role in natural inf...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2012.06.040
更新日期：2012-08-17 00:00:00
abstract：:Viral pandemics, such as the one caused by SARS-CoV-2, pose an imminent threat to humanity. Because of its recent emergence, there is a paucity of information regarding viral behavior and host response following SARS-CoV-2 infection. Here we offer an in-depth analysis of the transcriptional response to SARS-CoV-2 comp...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.04.026
更新日期：2020-05-28 00:00:00
