SARA-regulated vesicular targeting underlies formation of the light-sensing organelle in mammalian rods.

Abstract:

:The light-sensing organelle of the vertebrate rod photoreceptor, the outer segment (OS), is a modified cilium containing approximately 1,000 stacked disc membranes that are densely packed with visual pigment rhodopsin. The mammalian OS is renewed every ten days; new discs are assembled at the base of the OS by a poorly understood mechanism. Our results suggest that discs are formed and matured in a process that involves specific phospholipid-directed vesicular membrane targeting. Rhodopsin-laden vesicles in the OS axonemal cytoplasm fuse with nascent discs that are highly specialized with abundant phosphatidylinositol 3-phosphate (PI3P). This membrane coupling is regulated by the FYVE domain-containing protein, SARA, through its direct interaction with PI3P, rhodopsin, and SNARE protein syntaxin 3. Our model, in contrast to the previously proposed evagination model, suggests that the vesicular delivery of rhodopsin in the OS concentrates rhodopsin into discs, and this process directly participates in disc biogenesis.

journal_name

Cell

journal_title

Cell

authors

Chuang JZ,Zhao Y,Sung CH

doi

10.1016/j.cell.2007.06.030

subject

Has Abstract

pub_date

2007-08-10 00:00:00

pages

535-47

issue

3

eissn

0092-8674

issn

1097-4172

pii

S0092-8674(07)00827-6

journal_volume

130

pub_type

杂志文章

相关文献

CELL文献大全
  • Visualizing Intracellular Organelle and Cytoskeletal Interactions at Nanoscale Resolution on Millisecond Timescales.

    abstract::In eukaryotic cells, organelles and the cytoskeleton undergo highly dynamic yet organized interactions capable of orchestrating complex cellular functions. Visualizing these interactions requires noninvasive, long-duration imaging of the intracellular environment at high spatiotemporal resolution and low background. T...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2018.09.057

    authors: Guo Y,Li D,Zhang S,Yang Y,Liu JJ,Wang X,Liu C,Milkie DE,Moore RP,Tulu US,Kiehart DP,Hu J,Lippincott-Schwartz J,Betzig E,Li D

    更新日期:2018-11-15 00:00:00

  • The transcription factor titration effect dictates level of gene expression.

    abstract::Models of transcription are often built around a picture of RNA polymerase and transcription factors (TFs) acting on a single copy of a promoter. However, most TFs are shared between multiple genes with varying binding affinities. Beyond that, genes often exist at high copy number-in multiple identical copies on the c...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2014.02.022

    authors: Brewster RC,Weinert FM,Garcia HG,Song D,Rydenfelt M,Phillips R

    更新日期:2014-03-13 00:00:00

  • IKKalpha at the crossroads of inflammation and metastasis.

    abstract::The development of solid tumors is regulated by dynamic interactions between evolving neoplastic cells and their microenvironment. Luo et al. (2007) recently demonstrated that tumor-infiltrating immune cells expressing RANKL induce activation and nuclear localization of IKKalpha in prostatic epithelial tumor cells. Th...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2007.03.029

    authors: Affara NI,Coussens LM

    更新日期:2007-04-06 00:00:00

  • Phosphorylation-regulated binding of RNA polymerase II to fibrous polymers of low-complexity domains.

    abstract::The low-complexity (LC) domains of the products of the fused in sarcoma (FUS), Ewings sarcoma (EWS), and TAF15 genes are translocated onto a variety of different DNA-binding domains and thereby assist in driving the formation of cancerous cells. In the context of the translocated fusion proteins, these LC sequences fu...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2013.10.033

    authors: Kwon I,Kato M,Xiang S,Wu L,Theodoropoulos P,Mirzaei H,Han T,Xie S,Corden JL,McKnight SL

    更新日期:2013-11-21 00:00:00

  • The FIP1 gene encodes a component of a yeast pre-mRNA polyadenylation factor that directly interacts with poly(A) polymerase.

    abstract::We have identified an essential gene, called FIP1, encoding a 327 amino acid protein interacting with yeast poly(A) polymerase (PAP1) in the two-hybrid assay. Recombinant FIP1 protein forms a 1:1 complex with PAP1 in vitro. At 37 degrees C, a thermosensitive allele of FIP1 shows a shortening of poly(A) tails and a dec...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(95)90391-7

    authors: Preker PJ,Lingner J,Minvielle-Sebastia L,Keller W

    更新日期:1995-05-05 00:00:00

  • Developmental activation of the H-2K gene is correlated with an increase in DNA methylation.

    abstract::Embryonal carcinoma cell lines present strong developmental analogies with early embryonic cells. Previous studies have shown that treatment of F9 teratocarcinoma cells with retinoic acid induces the expression of the classical transplantation antigens which are indispensable for effective interactions between cells. ...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(83)90179-4

    authors: Tanaka K,Appella E,Jay G

    更新日期:1983-12-01 00:00:00

  • Inactivation of the mouse Msh2 gene results in mismatch repair deficiency, methylation tolerance, hyperrecombination, and predisposition to cancer.

    abstract::To investigate the role of the presumed DNA mismatch repair (MMR) gene Msh2 in genome stability and tumorigenesis, we have generated cells and mice that are deficient for the gene. Msh2-deficient cells have lost mismatch binding and have acquired microsatellite instability, a mutator phenotype, and tolerance to methyl...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(95)90319-4

    authors: de Wind N,Dekker M,Berns A,Radman M,te Riele H

    更新日期:1995-07-28 00:00:00

  • A Blueprint for Characterizing Senescence.

    abstract::Given the heterogeneity of senescent cells, our knowledge of both the drivers and consequences of cellular senescence in tissues and organs remains limited, as is our understanding of how this process could be harnessed for human health. Here we identified five broad areas that would help propel the field forward. ...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2020.10.032

    authors: Roy AL,Sierra F,Howcroft K,Singer DS,Sharpless N,Hodes RJ,Wilder EL,Anderson JM

    更新日期:2020-11-25 00:00:00

  • SGV1 encodes a CDC28/cdc2-related kinase required for a G alpha subunit-mediated adaptive response to pheromone in S. cerevisiae.

    abstract::The GPA1 gene of S. cerevisiae encodes a G alpha subunit that plays a positive role in the transduction of signals stimulating recovery from pheromone-induced cell cycle arrest. The GPA1Val50 mutation, in which Gly-50 is replaced by valine, causes hyperadaptation to pheromone. However, GPA1Val50 cells do not recover f...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(91)90386-d

    authors: Irie K,Nomoto S,Miyajima I,Matsumoto K

    更新日期:1991-05-31 00:00:00

  • Regulation of PKD by the MAPK p38delta in insulin secretion and glucose homeostasis.

    abstract::Dysfunction and loss of insulin-producing pancreatic beta cells represent hallmarks of diabetes mellitus. Here, we show that mice lacking the mitogen-activated protein kinase (MAPK) p38delta display improved glucose tolerance due to enhanced insulin secretion from pancreatic beta cells. Deletion of p38delta results in...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2008.11.018

    authors: Sumara G,Formentini I,Collins S,Sumara I,Windak R,Bodenmiller B,Ramracheya R,Caille D,Jiang H,Platt KA,Meda P,Aebersold R,Rorsman P,Ricci R

    更新日期:2009-01-23 00:00:00

  • Phosphodiesterase 4D deficiency in the ryanodine-receptor complex promotes heart failure and arrhythmias.

    abstract::Phosphodiesterases (PDEs) regulate the local concentration of 3',5' cyclic adenosine monophosphate (cAMP) within cells. cAMP activates the cAMP-dependent protein kinase (PKA). In patients, PDE inhibitors have been linked to heart failure and cardiac arrhythmias, although the mechanisms are not understood. We show that...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2005.07.030

    authors: Lehnart SE,Wehrens XH,Reiken S,Warrier S,Belevych AE,Harvey RD,Richter W,Jin SL,Conti M,Marks AR

    更新日期:2005-10-07 00:00:00

  • NF-κB c-Rel Is Crucial for the Regulatory T Cell Immune Checkpoint in Cancer.

    abstract::Regulatory T cells (Tregs) play a pivotal role in the inhibition of anti-tumor immune responses. Understanding the mechanisms governing Treg homeostasis may therefore be important for development of effective tumor immunotherapy. We have recently demonstrated a key role for the canonical nuclear factor κB (NF-κB) subu...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2017.08.004

    authors: Grinberg-Bleyer Y,Oh H,Desrichard A,Bhatt DM,Caron R,Chan TA,Schmid RM,Klein U,Hayden MS,Ghosh S

    更新日期:2017-09-07 00:00:00

  • SnapShot: Intrinsic Structural Disorder.

    abstract::Many proteins (intrinsically disordered proteins, IDPs) or regions of proteins (intrinsically disordered regions, IDRs) lack a well-defined 3D structure under physiological conditions. Albeit unfolded and highly dynamic, these proteins are not denatured; rather, intrinsic structural disorder is their native, functiona...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2015.05.024

    authors: Guharoy M,Pauwels K,Tompa P

    更新日期:2015-05-21 00:00:00

  • Phosphatidylinositol 4-phosphate 5-kinase alpha is a downstream effector of the small G protein ARF6 in membrane ruffle formation.

    abstract::Synthesis of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], a signaling phospholipid, is primarily carried out by phosphatidylinositol 4-phosphate 5-kinase [PI(4)P5K], which has been reported to be regulated by RhoA and Rac1. Unexpectedly, we find that the GTPgammaS-dependent activator of PI(4)P5Kalpha is the smal...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(00)81540-8

    authors: Honda A,Nogami M,Yokozeki T,Yamazaki M,Nakamura H,Watanabe H,Kawamoto K,Nakayama K,Morris AJ,Frohman MA,Kanaho Y

    更新日期:1999-11-24 00:00:00

  • Splice modulating therapies for human disease.

    abstract::Dysregulation of splicing and alternative splicing underlies many genetic and acquired diseases. We present an overview of recent strategies and successes in modulating splicing therapeutically in clinical and preclinical contexts. Effective approaches include restoring open reading frames, influencing alternative spl...

    journal_title:Cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.cell.2012.02.014

    authors: Spitali P,Aartsma-Rus A

    更新日期:2012-03-16 00:00:00

  • Coordinate transcription of variant surface glycoprotein genes and an expression site associated gene family in Trypanosoma brucei.

    abstract::Trypanosoma brucei variant surface glycoprotein (VSG) genes are activated either by duplicative (DA) transposition of the gene to a pre-activated expression site or by nonduplicative (NDA) activation of a previously silent telomeric gene. We have obtained a recombinant clone spanning the 5' barren region of the expres...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(85)80113-6

    authors: Cully DF,Ip HS,Cross GA

    更新日期:1985-08-01 00:00:00

  • Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways.

    abstract::Mammals can taste a wide repertoire of chemosensory stimuli. Two unrelated families of receptors (T1Rs and T2Rs) mediate responses to sweet, amino acids, and bitter compounds. Here, we demonstrate that knockouts of TRPM5, a taste TRP ion channel, or PLCbeta2, a phospholipase C selectively expressed in taste tissue, ab...

    journal_title:Cell

    pub_type: 评论,杂志文章

    doi:10.1016/s0092-8674(03)00071-0

    authors: Zhang Y,Hoon MA,Chandrashekar J,Mueller KL,Cook B,Wu D,Zuker CS,Ryba NJ

    更新日期:2003-02-07 00:00:00

  • Structure Studies of the CRISPR-Csm Complex Reveal Mechanism of Co-transcriptional Interference.

    abstract::Csm, a type III-A CRISPR-Cas interference complex, is a CRISPR RNA (crRNA)-guided RNase that also possesses target RNA-dependent DNase and cyclic oligoadenylate (cOA) synthetase activities. However, the structural features allowing target RNA-binding-dependent activation of DNA cleavage and cOA generation remain unkno...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2018.10.052

    authors: You L,Ma J,Wang J,Artamonova D,Wang M,Liu L,Xiang H,Severinov K,Zhang X,Wang Y

    更新日期:2019-01-10 00:00:00

  • Chromatin connections to pluripotency and cellular reprogramming.

    abstract::The pluripotent state of embryonic stem cells (ESCs) provides a unique perspective on regulatory programs that govern self-renewal and differentiation and somatic cell reprogramming. Here, we review the highly connected protein and transcriptional networks that maintain pluripotency and how they are intertwined with f...

    journal_title:Cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.cell.2011.05.019

    authors: Orkin SH,Hochedlinger K

    更新日期:2011-06-10 00:00:00

  • Crystal structure of human uracil-DNA glycosylase in complex with a protein inhibitor: protein mimicry of DNA.

    abstract::Uracil-DNA glycosylase inhibitor (Ugi) is a B. subtilis bacteriophage protein that protects the uracil-containing phage DNA by irreversibly inhibiting the key DNA repair enzyme uracil-DNA glycosylase (UDG). The 1.9 A crystal structure of Ugi complexed to human UDG reveals that the Ugi structure, consisting of a twiste...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(95)90467-0

    authors: Mol CD,Arvai AS,Sanderson RJ,Slupphaug G,Kavli B,Krokan HE,Mosbaugh DW,Tainer JA

    更新日期:1995-09-08 00:00:00

  • "Cloaking" on Time: A Cover-Up Act by Resident Tissue Macrophages.

    abstract::In this issue of Cell, Uderhardt et al. employed intravital two-photon microscopy to examine tissue-resident macrophage responses to sterile cellular injuries of variable size. They observed that while multi-cell "macrolesions" are characteristically pro-inflammatory, resident macrophages can "cloak" single-cell micro...

    journal_title:Cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.cell.2019.03.042

    authors: Blériot C,Ng LG,Ginhoux F

    更新日期:2019-04-18 00:00:00

  • The Striatum Organizes 3D Behavior via Moment-to-Moment Action Selection.

    abstract::Many naturalistic behaviors are built from modular components that are expressed sequentially. Although striatal circuits have been implicated in action selection and implementation, the neural mechanisms that compose behavior in unrestrained animals are not well understood. Here, we record bulk and cellular neural ac...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2018.04.019

    authors: Markowitz JE,Gillis WF,Beron CC,Neufeld SQ,Robertson K,Bhagat ND,Peterson RE,Peterson E,Hyun M,Linderman SW,Sabatini BL,Datta SR

    更新日期:2018-06-28 00:00:00

  • Structure of a heterophilic adhesion complex between the human CD2 and CD58 (LFA-3) counterreceptors.

    abstract::Interaction between CD2 and its counterreceptor, CD58 (LFA-3), on opposing cells optimizes immune recognition, facilitating contacts between helper T lymphocytes and antigen-presenting cells as well as between cytolytic effectors and target cells. Here, we report the crystal structure of the heterophilic adhesion comp...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(00)80790-4

    authors: Wang JH,Smolyar A,Tan K,Liu JH,Kim M,Sun ZY,Wagner G,Reinherz EL

    更新日期:1999-06-11 00:00:00

  • The retromer protein VPS29 links cell polarity and organ initiation in plants.

    abstract::A key feature of plants (as opposed to animals) is their ability to establish new organs not only during embryogenesis, but also throughout their development. A master regulator of organ initiation in plants is the phytohormone auxin. Auxin acts locally as a morphogen and is directionally transported from cell to cell...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2007.08.040

    authors: Jaillais Y,Santambrogio M,Rozier F,Fobis-Loisy I,Miège C,Gaude T

    更新日期:2007-09-21 00:00:00

  • Progressive loss of shape-responsive metabolic controls in cells with increasingly transformed phenotype.

    abstract::The modulation of cell metabolism by cell shape and external surface contact has been studied by suspension culture of anchorage-dependent fibroblasts. The suspended cells shut down protein synthesis and nuclear RNA metabolism and cease replicating DNA. However, these responses to suspension are lost or modified as ce...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(81)90111-2

    authors: Wittelsberger SC,Kleene K,Penman S

    更新日期:1981-06-01 00:00:00

  • MAC-1, a new genetically transmitted type C virus of primates: "low frequency" activation from stumptail monkey cell cultures.

    abstract::A new class of endogenous primate type C virus has been isolated from a continuous tissue culture line of Macaca arctoides cells by co-cultivation with a human cell line. The virus, designated MAC-1, can be transmitted to human and feline cells in tissue culture, and is unrelated, by immunological and nucleic acid hyb...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(78)90227-1

    authors: Todaro GJ,Benveniste RE,Sherwin SA,Sherr CJ

    更新日期:1978-04-01 00:00:00

  • Axonal remodeling and synaptic differentiation in the cerebellum is regulated by WNT-7a signaling.

    abstract::Synapse formation requires changes in cell morphology and the upregulation and localization of synaptic proteins. In the cerebellum, mossy fibers undergo extensive remodeling as they contact several granule cells and form complex, multisynaptic glomerular rosettes. Here we show that granule cells secrete factors that ...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(00)80689-3

    authors: Hall AC,Lucas FR,Salinas PC

    更新日期:2000-03-03 00:00:00

  • Nonreciprocal exchange between alleles of the yeast mitochondrial 21S rRNA gene: kinetics and the involvement of a double-strand break.

    abstract::A 1.1 kb intron containing an open reading frame (ORF) in one allele (omega+) of the yeast mitochondrial 21S rRNA gene is nearly quantitatively inserted in crosses into a 21S rRNA allele lacking that intron (omega-). We have determined that this nonreciprocal exchange initiates soon after cells fuse to form zygotes an...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(85)90348-4

    authors: Zinn AR,Butow RA

    更新日期:1985-04-01 00:00:00

  • Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging.

    abstract::Post-translational modifications of histone proteins and exchanges of histone variants of chromatin are central to the regulation of nearly all DNA-templated biological processes. However, the degree and variability of chromatin modifications in specific human immune cells remain largely unknown. Here, we employ a hig...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2018.03.079

    authors: Cheung P,Vallania F,Warsinske HC,Donato M,Schaffert S,Chang SE,Dvorak M,Dekker CL,Davis MM,Utz PJ,Khatri P,Kuo AJ

    更新日期:2018-05-31 00:00:00

  • A mechanism of formation of a persistent hybrid between elongating RNA and template DNA.

    abstract::At the replication origin of ColE1 plasmid, a persistent hybrid is formed between the primer precursor (RNA II) and its template DNA. The wild-type sequence in the region 13 to 20 bp upstream (-20 region) of the origin is required to form this persistent hybrid. While the template strand for transcription of this regi...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(90)90370-t

    authors: Masukata H,Tomizawa J

    更新日期:1990-07-27 00:00:00