Abstract：
:The Origin Recognition Complex (ORC) is a six-protein assembly that specifies the sites of DNA replication initiation in S. cerevisiae. Origin recognition by ORC requires ATP. Here, we demonstrate that two subunits, Orc1p and Orc5p, bind ATP and that Orc1p also hydrolyzes ATP. ATP binding and hydrolysis by Orc1p are both regulated by origin DNA in a sequence-specific manner. ATP binding to Orc1p, but not ATP hydrolysis, is responsible for the ATP dependence of the ORC-origin interaction, indicating that ATP is a cofactor that locks ORC on origin DNA. These data demonstrate that occupancy of the Orc1p ATP-binding site has a profound effect on ORC function and that ATP hydrolysis by Orc1p has the potential to drive transitions between different functional states of ORC.
journal_name
Celljournal_title
Cellauthors
Klemm RD,Austin RJ,Bell SPdoi
10.1016/s0092-8674(00)81889-9subject
Has Abstractpub_date
1997-02-21 00:00:00pages
493-502issue
4eissn
0092-8674issn
1097-4172pii
S0092-8674(00)81889-9journal_volume
88pub_type
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