Abstract:
:Nuclear DNA from eucaryotes contains a significant fraction which forms duplexes very rapidly and also independently of the DNA concentration. This fraction can be isolated by adsorption to hydroxylapatite and has been called foldback DNA (Britten and Smith, 1970). Here we extend previous studies to show that the foldback fraction is due to the existence of a finite number of foldback foci in each genome equivalent of DNA, approximately 10(5) in the case of Xenopus laevis. More significantly, we have isolated the foldback fraction in quantity from DNA of such a size (in one case broken randomly and in another digested with a restriction endonuclease) that only about 10% of the total DNA has foldback properties. If the foldback foci were located in precisely the same positions in all sets of the Xenopus laevis genome, the prediction would be that these foldback fractions would contain sequences representing 20% (random shear) and 10% (restriction endonuclease) of the total genome. In contrast, our results show that in both cases the foldback fraction contains the entire Xenopus laevis DNA sequence. One possible explanation of these observations is that as in procaryotes, eucaryotic DNA is randomly cross-linked. We show that cross-linkage of Xenopus laevis DNA is not sufficient to explain our observations. In consequence, we have adopted the hypothesis that the formation of foldback DNA is mainly an intrastrand phenomenon, but nevertheless occurs at different sites in different sets of the Xenopus laevis genome.
journal_name
Celljournal_title
Cellauthors
Perlman S,Phillips C,Bishop JOdoi
10.1016/0092-8674(76)90182-3subject
Has Abstractpub_date
1976-05-01 00:00:00pages
33-42issue
1eissn
0092-8674issn
1097-4172pii
0092-8674(76)90182-3journal_volume
8pub_type
杂志文章相关文献
CELL文献大全abstract::Individual olfactory sensory neurons express only a single odorant receptor from a large family of genes, and this singularity is an essential feature in models of olfactory perception. We have devised a genetic strategy to examine the stability of receptor choice. We observe that immature olfactory sensory neurons th...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2004.05.015
更新日期:2004-06-11 00:00:00
abstract::Endoplasmic reticulum (ER) membrane contact sites (MCSs) mark positions where endosomes undergo fission for cargo sorting. To define the role of ER at this unique MCS, we targeted a promiscuous biotin ligase to cargo-sorting domains on endosome buds. This strategy identified the ER membrane protein TMCC1, a member of ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.08.030
更新日期:2018-09-20 00:00:00
abstract::The first event in the initiation of eukaryotic DNA replication is the recruitment of the MCM2-7 ATPase, the core of the replicative DNA helicase, to origins. Ticau et al. use single-molecule imaging to reveal how ORC, Cdc6, and Cdt1 cooperate to load MCM2-7 onto DNA, enabling bidirectional replication. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2015.04.006
更新日期:2015-04-23 00:00:00
abstract::Plasmids expressing partial-length sense or antisense protein 4.1 RNA were microinjected into fertilized Xenopus eggs. Nuclease protection assays reveal that antisense protein 4.1 RNA lead to the specific loss of endogenous protein 4.1 transcripts after midblastula transition, with no effect on the levels of three unr...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(88)90576-4
更新日期:1988-05-20 00:00:00
abstract::The mitotic checkpoint ensures proper chromosome segregation by delaying anaphase until chromosomes are aligned on the spindle. Following prolonged spindle damage, however, cells eventually exit mitosis and undergo apoptosis. We show here that a murine homolog of the yeast mitotic checkpoint gene BUB1 localizes to the...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80255-x
更新日期:1997-05-30 00:00:00
abstract::Assessing the real-world impact of biomedical research is notoriously difficult. Here, we present the framework for building a prospective science-centered information system from scratch that has been afforded by the Sidra Medical and Research Center in Qatar. This experiment is part of the global conversation on max...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.03.004
更新日期:2016-03-24 00:00:00
abstract::The CD4 lymphocyte surface glycoprotein and the lck tyrosine protein kinase p56lck are found as a complex in T lymphocytes. We have defined the domains in both proteins that are responsible for this interaction by coexpressing hybrid and deleted forms of the two proteins in HeLa cells. We have found that the unique 32...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90008-1
更新日期:1989-11-17 00:00:00
abstract::We previously demonstrated that pseudogenes complementary to the small nuclear RNAs U1, U2 and U3 are dispersed and abundant in the human genome. Here we report that three pseudogenes (U1.101, U2.13 and U3.5) are flanked by perfect short direct repeats of 16 to 19 base pairs. In all three pseudogenes. the upstream dir...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90028-3
更新日期:1981-10-01 00:00:00
abstract::Phagosomes were traditionally thought to originate from an invagination and scission of the plasma membrane to form a distinct intracellular vacuole. An alternative model implicating the endoplasmic reticulum (ER) as a major component of nascent and maturing phagosomes was recently proposed (Gagnon et al., 2002). To r...
journal_title:Cell
pub_type: 信件
doi:10.1016/j.cell.2005.08.018
更新日期:2005-10-07 00:00:00
abstract::The assembly of COPI into a cage-like lattice sculpts membrane vesicles that transport cargo from the Golgi apparatus. Now, Lee and Goldberg (2010) present X-ray crystal structures of COPI suggesting that these coats combine selected features of two other archetypal coats, clathrin and COPII. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2010.06.031
更新日期:2010-07-09 00:00:00
abstract::Dominantly inherited mutations in an endoplasmic reticulum protein called VAPB have been found in a subset of patients with a rare familial form of amyotrophic lateral sclerosis (ALS). In this issue, Tsuda et al. (2008) identify a secreted form of VAPB that binds directly to Eph receptors inducing their activation and...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2008.05.028
更新日期:2008-06-13 00:00:00
abstract::Gastrointestinal enterochromaffin cells regulate bone and gut homeostasis via serotonin (5-hydroxytryptamine [5-HT]) production. A recent report suggested that gut microbes regulate 5-HT levels; however, the precise underlying molecular mechanisms are unexplored. Here, we reveal that the cation channel Piezo1 in the g...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.06.022
更新日期:2020-08-06 00:00:00
abstract::Serum response factor (SRF) forms a ternary complex at the c-fos serum response element (SRE) with an accessory factor, Elk-1. We constructed altered-binding specificity derivatives of SRF and Elk-1 that form a ternary complex at a mutated, inactive SRE; like Elk-1, the Elk-1 variant only binds its target as part of a...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90238-l
更新日期:1993-04-23 00:00:00
abstract::During the process of chromatin diminution, which takes place in all presomatic cells of the early Ascaris embryo, the heterochromatic termini of the chromosomes are lost. Here we show that the newly formed ends of the reduced somatic chromosomes carry tandem repeats of the telomeric sequence TTAGGC. Comparison of a c...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90076-b
更新日期:1991-11-15 00:00:00
abstract::Subcellular compartments in which folding and assembly of proteins occur seem to have a set of PCB proteins capable of mediating these and related processes, such as translocation across membranes. When a domain of a polypeptide chain emerges from a ribosome during synthesis or from the distal side of a membrane durin...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/0092-8674(89)90005-6
更新日期:1989-11-17 00:00:00
abstract::Recent studies suggest that local translation of mRNA within axons plays a key role in two aspects of growth cone navigation. One line of evidence implies a local synthesis of proteins that mediate growth cone collapse and turning in response to guidance cues; another indicates that growth cones possess the machinery ...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/s0092-8674(02)00934-0
更新日期:2002-09-06 00:00:00
abstract::Nucleotide sequences in two wild-type and six mutant operators in the DNA of phage lambda are compared. Strikingly similar 17 base pair units are found which we identify as the repressor binding sites. Each operator contains multiple repressor binding sites separated by A-T rich spacers. Elements of 2 fold rotational ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(75)90018-5
更新日期:1975-06-01 00:00:00
abstract::PD-1 immune checkpoint blockade provides significant clinical benefits for melanoma patients. We analyzed the somatic mutanomes and transcriptomes of pretreatment melanoma biopsies to identify factors that may influence innate sensitivity or resistance to anti-PD-1 therapy. We find that overall high mutational loads a...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2016.02.065
更新日期:2016-03-24 00:00:00
abstract::DNA transcription, replication, and repair are regulated by histone acetylation, a process that requires the generation of acetyl-coenzyme A (CoA). Here, we show that all the subunits of the mitochondrial pyruvate dehydrogenase complex (PDC) are also present and functional in the nucleus of mammalian cells. We found t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.04.046
更新日期:2014-07-03 00:00:00
abstract::We describe a monoclonal antibody (anti-FC-1) that inhibits the attachment of chicken embryo fibroblasts to artificial substrates. In the immunofluorescence, anti-FC-1 also stained the focal contacts of the fibroblasts, organelles suggested to be involved in cell-substrate adhesion. This antibody was absorbed on the s...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90322-1
更新日期:1982-12-01 00:00:00
abstract::We have identified mutations in Drosophila endophilin to study its function in vivo. Endophilin is required presynaptically at the neuromuscular junction, and absence of Endophilin dramatically impairs endocytosis in vivo. Mutant larvae that lack Endophilin fail to take up FM1-43 dye in synaptic boutons, indicating an...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(02)00688-8
更新日期:2002-04-05 00:00:00
abstract::Addition of LETS glycoprotein to normal or transformed cells produces increased migration of the cells, as determined by formation of phagokinetic tracks on gold particle-coated coverslips. These tracks arise by a combination of phagocytosis of the gold particles and cellular migration. Increased motility is also evid...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90129-0
更新日期:1978-06-01 00:00:00
abstract::The chicken immunoglobulin light chain repertoire has been shown to be entirely derived from a single V lambda 1-J rearranged combination. The complete coding information of the lambda locus was determined: it comprises 25 V-hybridizing elements, all of which are pseudogenes, clustered in both orientations within 19 k...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90189-9
更新日期:1987-02-13 00:00:00
abstract::The rabbit alpha-globin DNA insertion in the chimeric plasmid pHb 72 (Liu et al., 1977) has been sequenced by the method of Maxam and Gilbert (1977). This has enabled us to determine the messenger RNA(mRNA) sequence beginning in the 5' untranslated region 9 nucleotides before the initiation codon and extending through...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90081-8
更新日期:1978-09-01 00:00:00
abstract::Exit from mitosis must not occur prior to partitioning of chromosomes between daughter cells. We find that the GTP binding protein Tem1, a regulator of mitotic exit, is present on the spindle pole body that migrates into the bud during S phase and mitosis. Tem1's exchange factor, Lte1, localizes to the bud. Thus, Tem1...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)00007-6
更新日期:2000-07-07 00:00:00
abstract::During cell division, newly replicated DNA is actively segregated to the daughter cells. In most bacteria, this process involves the DNA-binding protein ParB, which condenses the centromeric regions of sister DNA molecules into kinetochore-like structures that recruit the DNA partition ATPase ParA and the prokaroytic ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.11.015
更新日期:2019-12-12 00:00:00
abstract::Pancreatic cancer is one of the most lethal malignancies due to its late diagnosis and limited response to treatment. Tractable methods to identify and interrogate pathways involved in pancreatic tumorigenesis are urgently needed. We established organoid models from normal and neoplastic murine and human pancreas tiss...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.12.021
更新日期:2015-01-15 00:00:00
abstract::The TDS4 gene of S. cerevisiae was isolated as an allele-specific high copy suppressor of mutations within the basic region of the TATA-binding protein (TBP). The gene is essential for viability and encodes a 596 aa protein. The first 300 aa of the TDS4 protein exhibit significant sequence similarity to the RNA polyme...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90351-c
更新日期:1992-10-16 00:00:00
abstract::Xenogeneic spinal cord chimeras were constructed by grafting fragments of quail neural primordium into chick embryos at 2 days of incubation. Hatched birds displayed normal motor behavior for about 5 to 7 weeks, whereupon they developed a neurological syndrome; in the grafted spinal cord the pathological signs of the ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90395-8
更新日期:1986-04-25 00:00:00
abstract::Mutations at the mouse tottering (tg) locus cause a delayed-onset, recessive neurological disorder resulting in ataxia, motor seizures, and behavioral absence seizures resembling petit mal epilepsy in humans. A more severe allele, leaner (tg(la)), also shows a slow, selective degeneration of cerebellar neurons. By pos...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81381-1
更新日期:1996-11-15 00:00:00