DNAase I encapsulated in liposomes can induce neoplastic transformation of Syrian hamster embryo cells in culture.

Abstract:

:We have used liposomes to deliver DNAase I inside normal Syrian hamster embryo (SHE) cells. We showed the entrance of DNAase I inside the cell by dose-dependent cytotoxicity; and the entrance of DNAase I into the nucleus by the induction of chromosomal aberrations and somatic mutation at the HPRT locus (but not at the Na+/K+ ATPase locus). The induction of neoplastic transformation in cultures treated by DNAase I-in-liposomes was manifested by increased saturation density, colony formation at low seeding density, colony formation in 1% serum and 0.3% agar, and tumorigenicity in 100% of injected animals. The acquisition of anchorage-independent growth became apparent only after 39-57 posttreatment population doublings. Thus damage to DNA alone can initiate the neoplastic transformation process; but for full expression of the neoplastic phenotypes, a long progression time is required for the acquisition of anchorage-independent growth and tumorigenicity.

journal_name

Cell

journal_title

Cell

authors

Zajac-Kaye M,Ts'o PO

doi

10.1016/0092-8674(84)90450-1

subject

Has Abstract

pub_date

1984-12-01 00:00:00

pages

427-37

issue

3 Pt 2

eissn

0092-8674

issn

1097-4172

pii

0092-8674(84)90450-1

journal_volume

39

pub_type

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