DNAase I encapsulated in liposomes can induce neoplastic transformation of Syrian hamster embryo cells in culture.


:We have used liposomes to deliver DNAase I inside normal Syrian hamster embryo (SHE) cells. We showed the entrance of DNAase I inside the cell by dose-dependent cytotoxicity; and the entrance of DNAase I into the nucleus by the induction of chromosomal aberrations and somatic mutation at the HPRT locus (but not at the Na+/K+ ATPase locus). The induction of neoplastic transformation in cultures treated by DNAase I-in-liposomes was manifested by increased saturation density, colony formation at low seeding density, colony formation in 1% serum and 0.3% agar, and tumorigenicity in 100% of injected animals. The acquisition of anchorage-independent growth became apparent only after 39-57 posttreatment population doublings. Thus damage to DNA alone can initiate the neoplastic transformation process; but for full expression of the neoplastic phenotypes, a long progression time is required for the acquisition of anchorage-independent growth and tumorigenicity.






Zajac-Kaye M,Ts'o PO




Has Abstract


1984-12-01 00:00:00




3 Pt 2












  • The ClpXP protease unfolds substrates using a constant rate of pulling but different gears.

    abstract::ATP-dependent proteases are vital to maintain cellular protein homeostasis. Here, we study the mechanisms of force generation and intersubunit coordination in the ClpXP protease from E. coli to understand how these machines couple ATP hydrolysis to mechanical protein unfolding. Single-molecule analyses reveal that pho...


    pub_type: 杂志文章


    authors: Sen M,Maillard RA,Nyquist K,Rodriguez-Aliaga P,Pressé S,Martin A,Bustamante C

    更新日期:2013-10-24 00:00:00

  • Preclinical mouse cancer models: a maze of opportunities and challenges.

    abstract::Significant advances have been made in developing novel therapeutics for cancer treatment, and targeted therapies have revolutionized the treatment of some cancers. Despite the promise, only about five percent of new cancer drugs are approved, and most fail due to lack of efficacy. The indication is that current precl...


    pub_type: 杂志文章,评审


    authors: Day CP,Merlino G,Van Dyke T

    更新日期:2015-09-24 00:00:00

  • Naturally Occurring Off-Switches for CRISPR-Cas9.

    abstract::CRISPR-Cas9 technology would be enhanced by the ability to inhibit Cas9 function spatially, temporally, or conditionally. Previously, we discovered small proteins encoded by bacteriophages that inhibit the CRISPR-Cas systems of their host bacteria. These "anti-CRISPRs" were specific to type I CRISPR-Cas systems that d...


    pub_type: 评论,杂志文章


    authors: Pawluk A,Amrani N,Zhang Y,Garcia B,Hidalgo-Reyes Y,Lee J,Edraki A,Shah M,Sontheimer EJ,Maxwell KL,Davidson AR

    更新日期:2016-12-15 00:00:00

  • Interaction of endothelin-3 with endothelin-B receptor is essential for development of epidermal melanocytes and enteric neurons.

    abstract::Defects in the gene encoding the endothelin-B receptor produce aganglionic megacolon and pigmentary disorders in mice and humans. We report that a targeted disruption of the mouse endothelin-3 ligand (EDN3) gene produces a similar recessive phenotype of megacolon and coat color spotting. A natural recessive mutation t...


    pub_type: 杂志文章


    authors: Baynash AG,Hosoda K,Giaid A,Richardson JA,Emoto N,Hammer RE,Yanagisawa M

    更新日期:1994-12-30 00:00:00

  • The mechanism of RNA recombination in poliovirus.

    abstract::We have investigated RNA recombination among poliovirus genomes by analyzing both intratypic and intertypic recombinant crosses involving the same defined genetic markers. Sequence analysis of the recombinant junctions of 13 nonsibling intertypic recombinants showed that intertypic RNA recombination is not site-specif...


    pub_type: 杂志文章


    authors: Kirkegaard K,Baltimore D

    更新日期:1986-11-07 00:00:00

  • The role of Fas in autoimmune diabetes.

    abstract::Immunologically privileged sites express Fas ligand (FasL), which protects them from attack by activated T cells that express Fas and die upon contact with FasL. In an attempt to protect nonobese diabetic mice (NOD) from autoimmune diabetes, we made FasL transgenic NOD mice using the beta cell-specific rat insulin-1 p...


    pub_type: 杂志文章


    authors: Chervonsky AV,Wang Y,Wong FS,Visintin I,Flavell RA,Janeway CA Jr,Matis LA

    更新日期:1997-04-04 00:00:00

  • DBP, a liver-enriched transcriptional activator, is expressed late in ontogeny and its tissue specificity is determined posttranscriptionally.

    abstract::The full-length cDNA for a transcriptional activator, DBP, that binds to the D site of the albumin promoter has been cloned. DBP belongs to a family of related transcription factors including Fos, Jun, CREB, and C/EBP, which share a conserved basic domain. However, unlike most other members of this family, DBP does no...


    pub_type: 杂志文章


    authors: Mueller CR,Maire P,Schibler U

    更新日期:1990-04-20 00:00:00

  • Site-specific base substitution and deletion mutations that enhance or suppress transcription of the SV40 major late RNA.

    abstract::Transcriptional analysis of SV40 late promoter mutants indicates that the DNA sequence 5'-GGTACCTAACC-3' (map positions 294-304) is important in the control of SV40 late RNA expression. C to T base substitutions at map positions 298, 299 and 304 increased initiation of late RNA synthesis at map position 325 five to te...


    pub_type: 杂志文章


    authors: Brady J,Radonovich M,Vodkin M,Natarajan V,Thoren M,Das G,Janik J,Salzman NP

    更新日期:1982-12-01 00:00:00

  • Genetic changes in skin tumor progression: correlation between presence of a mutant ras gene and loss of heterozygosity on mouse chromosome 7.

    abstract::Initiation of tumorigenesis in mouse skin can be accomplished by mutagenesis of the H-ras gene by treatment with chemical carcinogens. A mouse model system has been developed to study the additional genetic events that take place during tumor progression. Skin carcinomas were induced in F1 hybrid mice exhibiting restr...


    pub_type: 杂志文章


    authors: Bremner R,Balmain A

    更新日期:1990-05-04 00:00:00

  • Identification of F-actin as the dynamic hub in a microbial-induced GTPase polarity circuit.

    abstract::Polarity in mammalian cells emerges from the assembly of signaling molecules into extensive biochemical interaction networks. Despite their complexity, bacterial pathogens have evolved parsimonious mechanisms to hijack these systems. Here, we develop a tractable experimental and theoretical model to uncover fundamenta...


    pub_type: 杂志文章


    authors: Orchard RC,Kittisopikul M,Altschuler SJ,Wu LF,Süel GM,Alto NM

    更新日期:2012-02-17 00:00:00

  • Nonreciprocal exchange between alleles of the yeast mitochondrial 21S rRNA gene: kinetics and the involvement of a double-strand break.

    abstract::A 1.1 kb intron containing an open reading frame (ORF) in one allele (omega+) of the yeast mitochondrial 21S rRNA gene is nearly quantitatively inserted in crosses into a 21S rRNA allele lacking that intron (omega-). We have determined that this nonreciprocal exchange initiates soon after cells fuse to form zygotes an...


    pub_type: 杂志文章


    authors: Zinn AR,Butow RA

    更新日期:1985-04-01 00:00:00

  • Single-Cell Analysis of Crohn's Disease Lesions Identifies a Pathogenic Cellular Module Associated with Resistance to Anti-TNF Therapy.

    abstract::Clinical benefits of cytokine blockade in ileal Crohn's disease (iCD) are limited to a subset of patients. Here, we applied single-cell technologies to iCD lesions to address whether cellular heterogeneity contributes to treatment resistance. We found that a subset of patients expressed a unique cellular module in inf...


    pub_type: 杂志文章


    authors: Martin JC,Chang C,Boschetti G,Ungaro R,Giri M,Grout JA,Gettler K,Chuang LS,Nayar S,Greenstein AJ,Dubinsky M,Walker L,Leader A,Fine JS,Whitehurst CE,Mbow ML,Kugathasan S,Denson LA,Hyams JS,Friedman JR,Desai PT,Ko

    更新日期:2019-09-05 00:00:00

  • tudor, a gene required for assembly of the germ plasm in Drosophila melanogaster.

    abstract::Developmental analysis of a newly isolated maternal effect grandchildless mutant, tudor (tud), in Drosophila melanogaster indicates that tud+ activity is required during oogenesis for the determination and/or formation of primordial germ cells (pole cells) and for normal embryonic abdominal segmentation. Regardless of...


    pub_type: 杂志文章


    authors: Boswell RE,Mahowald AP

    更新日期:1985-11-01 00:00:00

  • Signal peptides open protein-conducting channels in E. coli.

    abstract::Plasma membrane vesicles and protoplasts of Escherichia coli were fused to planar lipid bilayers and studied with electrophysiological techniques. Large transmembrane aqueous channels were opened when 0.2 nM LamB signal peptide was added to the cytoplasmic side of the membrane. These aqueous pores are similar in condu...


    pub_type: 杂志文章


    authors: Simon SM,Blobel G

    更新日期:1992-05-15 00:00:00

  • A transient, neuron-wide form of CREB-mediated long-term facilitation can be stabilized at specific synapses by local protein synthesis.

    abstract::In a culture system where a bifurcated Aplysia sensory neuron makes synapses with two motor neurons, repeated application of serotonin (5-HT) to one synapse produces a CREB-mediated, synapse-specific, long-term facilitation, which can be captured at the opposite synapse by a single pulse of 5-HT. Repeated pulses of 5-...


    pub_type: 杂志文章


    authors: Casadio A,Martin KC,Giustetto M,Zhu H,Chen M,Bartsch D,Bailey CH,Kandel ER

    更新日期:1999-10-15 00:00:00

  • An extensively edited mitochondrial transcript in kinetoplastids encodes a protein homologous to ATPase subunit 6.

    abstract::The mitochondrial MURF4 gene of T. brucei has pronounced G versus C strand bias and heterogeneously sized transcripts, characteristic of genes encoding extensively edited transcripts. We find that MURF4 transcripts of T. brucei are extensively edited throughout by the addition and deletion of numerous uridines, creati...


    pub_type: 杂志文章


    authors: Bhat GJ,Koslowsky DJ,Feagin JE,Smiley BL,Stuart K

    更新日期:1990-06-01 00:00:00

  • Exposing secrets of telomere-telomerase encounters.

    abstract::In order for telomeres to remain functional and stable, they must rendezvous with the enzyme telomerase in a productive manner. In human cells, this interaction is mediated by Cajal bodies as matchmaker, and now Zhong et al. reveal molecular determinants that establish good chemistry between the two partners. ...


    pub_type: 评论,杂志文章


    authors: Noël JF,Wellinger RJ

    更新日期:2012-08-03 00:00:00

  • The nuclear tyrosine kinase c-Abl negatively regulates cell growth.

    abstract::c-Abl is a tyrosine kinase localized primarily in the nucleus. Previous assays for abl function rely on cellular transformation by abl mutants, which are cytoplasmic. Using a conditional overexpression strategy, we have developed a functional assay for c-abl. Overexpression of c-abl inhibits growth by causing cell cyc...


    pub_type: 杂志文章


    authors: Sawyers CL,McLaughlin J,Goga A,Havlik M,Witte O

    更新日期:1994-04-08 00:00:00

  • Muscle differentiation in normal and cleavage-arrested mutant embryos of Caenorhabditis elegans.

    abstract::The differentiation of body-wall muscle cells was studied in the nematode Caenorhabditis elegans. Specific antibodies to myosin and paramyosin, major protein constituents of differentiated muscle, react with mesodermal cells in wild-type embryos towards the end of the first half of embryogenesis. Immunoreactive cells ...


    pub_type: 杂志文章


    authors: Gossett LA,Hecht RM,Epstein HF

    更新日期:1982-08-01 00:00:00

  • MITRAC links mitochondrial protein translocation to respiratory-chain assembly and translational regulation.

    abstract::Mitochondrial respiratory-chain complexes assemble from subunits of dual genetic origin assisted by specialized assembly factors. Whereas core subunits are translated on mitochondrial ribosomes, others are imported after cytosolic translation. How imported subunits are ushered to assembly intermediates containing mito...


    pub_type: 杂志文章


    authors: Mick DU,Dennerlein S,Wiese H,Reinhold R,Pacheu-Grau D,Lorenzi I,Sasarman F,Weraarpachai W,Shoubridge EA,Warscheid B,Rehling P

    更新日期:2012-12-21 00:00:00

  • Ubiquitylation of the 9-1-1 checkpoint clamp is independent of rad6-rad18 and DNA damage.

    abstract::A recent report proposed a function of the ubiquitin conjugation factors Rad6 and Rad18 comparable to the bacterial SOS response, controlling damage-induced transcriptional activation and contributing to checkpoint signaling. The relevant ubiquitylation target was identified as budding yeast Rad17, a subunit of the PC...


    pub_type: 杂志文章


    authors: Davies AA,Neiss A,Ulrich HD

    更新日期:2010-06-11 00:00:00

  • Origins and fates of cardiovascular progenitor cells.

    abstract::Multipotent cardiac progenitor cells are found in the fetal and adult heart of many mammalian species including humans and form as intermediates during the differentiation of embryonic stem cells. Despite similar biological properties, the molecular identities of these different cardiac progenitor cell populations app...


    pub_type: 杂志文章


    authors: Wu SM,Chien KR,Mummery C

    更新日期:2008-02-22 00:00:00

  • A nonpolymorphic class I gene in the murine major histocompatibility complex.

    abstract::DNA sequence analysis of a class I gene (Q10), which maps to the Qa2,3 locus in the C57BL/10 (H-2b haplotype) mouse, reveals that it is almost identical to a cDNA clone (pH16) isolated from a SWR/J (H-2q haplotype) mouse liver cDNA library. Exon 5, in particular, has an unusual structure such that a polypeptide produc...


    pub_type: 杂志文章


    authors: Mellor AL,Weiss EH,Kress M,Jay G,Flavell RA

    更新日期:1984-01-01 00:00:00

  • Positioning the Golgi apparatus.

    abstract::Ríos et al. (2004) report in this issue that the Golgi protein GMAP-210 is sufficient to confer pericentrosomal positioning and recruits gamma-tubulin and associated microtubule-nucleating ring complex proteins to Golgi membranes. The results raise the possibility that short microtubules emanate from the Golgi to medi...


    pub_type: 评论,杂志文章


    authors: Linstedt AD

    更新日期:2004-08-06 00:00:00

  • Efficient direct reprogramming of mature amniotic cells into endothelial cells by ETS factors and TGFβ suppression.

    abstract::ETS transcription factors ETV2, FLI1, and ERG1 specify pluripotent stem cells into induced vascular endothelial cells (iVECs). However, iVECs are unstable and drift toward nonvascular cells. We show that human midgestation c-Kit(-) lineage-committed amniotic cells (ACs) can be reprogrammed into vascular endothelial ce...


    pub_type: 杂志文章


    authors: Ginsberg M,James D,Ding BS,Nolan D,Geng F,Butler JM,Schachterle W,Pulijaal VR,Mathew S,Chasen ST,Xiang J,Rosenwaks Z,Shido K,Elemento O,Rabbany SY,Rafii S

    更新日期:2012-10-26 00:00:00

  • Separation of endocytic from exocytic coated vesicles using a novel cholinesterase mediated density shift technique.

    abstract::Coated vesicles isolated from rat liver perfused with diisopropylfluorophosphate (DFP) to inactivate endogenous cholinesterase contained newly synthesized secretory cholinesterase after a 30 min recovery. The cholinesterase is found in coated vesicles of presumed endocytic origin following DFP treatment and perfusion ...


    pub_type: 杂志文章


    authors: Helmy S,Porter-Jordan K,Dawidowicz EA,Pilch P,Schwartz AL,Fine RE

    更新日期:1986-02-14 00:00:00

  • Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile.

    abstract::We present a strategy for generating and analyzing comprehensive genetic-interaction maps, termed E-MAPs (epistatic miniarray profiles), comprising quantitative measures of aggravating or alleviating interactions between gene pairs. Crucial to the interpretation of E-MAPs is their high-density nature made possible by ...


    pub_type: 杂志文章


    authors: Schuldiner M,Collins SR,Thompson NJ,Denic V,Bhamidipati A,Punna T,Ihmels J,Andrews B,Boone C,Greenblatt JF,Weissman JS,Krogan NJ

    更新日期:2005-11-04 00:00:00

  • Expression of cDNA encoding the human "protective protein" associated with lysosomal beta-galactosidase and neuraminidase: homology to yeast proteases.

    abstract::The "protective protein" is a glycoprotein that associates with lysosomal beta-galactosidase and neuraminidase and is deficient in the autosomal recessive disorder galactosialidosis. We have isolated the cDNA encoding human "protective protein". The clone recognizes a 2 kb mRNA in normal cells that is not evident in f...


    pub_type: 杂志文章


    authors: Galjart NJ,Gillemans N,Harris A,van der Horst GT,Verheijen FW,Galjaard H,d'Azzo A

    更新日期:1988-09-09 00:00:00

  • The Antagonistic Gene Paralogs Upf3a and Upf3b Govern Nonsense-Mediated RNA Decay.

    abstract::Gene duplication is a major evolutionary force driving adaptation and speciation, as it allows for the acquisition of new functions and can augment or diversify existing functions. Here, we report a gene duplication event that yielded another outcome--the generation of antagonistic functions. One product of this dupli...


    pub_type: 杂志文章


    authors: Shum EY,Jones SH,Shao A,Dumdie J,Krause MD,Chan WK,Lou CH,Espinoza JL,Song HW,Phan MH,Ramaiah M,Huang L,McCarrey JR,Peterson KJ,De Rooij DG,Cook-Andersen H,Wilkinson MF

    更新日期:2016-04-07 00:00:00

  • Common PIEZO1 Allele in African Populations Causes RBC Dehydration and Attenuates Plasmodium Infection.

    abstract::Hereditary xerocytosis is thought to be a rare genetic condition characterized by red blood cell (RBC) dehydration with mild hemolysis. RBC dehydration is linked to reduced Plasmodium infection in vitro; however, the role of RBC dehydration in protection against malaria in vivo is unknown. Most cases of hereditary xer...


    pub_type: 杂志文章


    authors: Ma S,Cahalan S,LaMonte G,Grubaugh ND,Zeng W,Murthy SE,Paytas E,Gamini R,Lukacs V,Whitwam T,Loud M,Lohia R,Berry L,Khan SM,Janse CJ,Bandell M,Schmedt C,Wengelnik K,Su AI,Honore E,Winzeler EA,Andersen KG,Patapou

    更新日期:2018-04-05 00:00:00