Abstract:
:Homologous recombination in embryonal stem cells has been used to produce a fusion oncogene, thereby mimicking chromosomal translocations that frequently result in formation of tumor-specific fusion oncogenes in human malignancies. AF9 sequences were fused into the mouse Mll gene so that expression of the Mll-AF9 fusion gene occurred from endogenous Mll transcription control elements, as in t(9;11) found in human leukemias. Chimeric mice carrying the fusion gene developed tumors, which were restricted to acute myeloid leukemias despite the widespread activity of the Mll promoter. Onset of perceptible disease was preceded by expansion of ES cell derivatives in peripheral blood. This novel use of homologous recombination formally proves that chromosomal translocations contribute to malignancy and provides a general strategy to create fusion oncogenes for studying their role in tumorigenesis.
journal_name
Celljournal_title
Cellauthors
Corral J,Lavenir I,Impey H,Warren AJ,Forster A,Larson TA,Bell S,McKenzie AN,King G,Rabbitts THdoi
10.1016/s0092-8674(00)81269-6subject
Has Abstractpub_date
1996-06-14 00:00:00pages
853-61issue
6eissn
0092-8674issn
1097-4172pii
S0092-8674(00)81269-6journal_volume
85pub_type
杂志文章相关文献
CELL文献大全abstract::Transcription termination by bacterial RNA polymerase (RNAP) occurs at sequences coding for a GC-rich RNA hairpin followed by a U-rich tract. We used single-molecule techniques to investigate the mechanism by which three representative terminators (his, t500, and tR2) destabilize the elongation complex (EC). For his a...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2008.01.027
更新日期:2008-03-21 00:00:00
abstract::Recent evidence suggests that a subset of cancer cells within some tumors, the so-called cancer stem cells, may drive the growth and metastasis of these tumors. Understanding the pathways that regulate proliferation, self-renewal, survival, and differentiation of malignant and normal stem cells may shed light on mecha...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.03.011
更新日期:2006-03-24 00:00:00
abstract::We have adopted Drosophila tissue culture cells as a host system for studying the structure and function of mammalian transcription factors. These cells provide an Sp1-deficient background and have been used in a complementation assay to identify functional domains of human transcription factor Sp1. The SV40 early pro...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(88)90144-4
更新日期:1988-12-02 00:00:00
abstract::Animals learn to avoid harmful situations by associating a neutral stimulus with a painful one, resulting in a stable threat memory. In mammals, this form of learning requires the amygdala. Although pain is the main driver of aversive learning, the mechanism that transmits pain signals to the amygdala is not well reso...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.05.057
更新日期:2015-07-16 00:00:00
abstract::To search for histone domains that may regulate transcription in vivo, we made deletions and amino acid substitutions in the histone N-termini of S. cerevisiae. Histone H4 N-terminal residues 4-23, which include the extremely conserved, reversibly acetylated lysines (at positions 5, 8, 12, and 16), were found to encom...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90554-c
更新日期:1991-06-14 00:00:00
abstract::To guide the design of immunotherapy strategies for patients with early stage lung tumors, we developed a multiscale immune profiling strategy to map the immune landscape of early lung adenocarcinoma lesions to search for tumor-driven immune changes. Utilizing a barcoding method that allows a simultaneous single-cell ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.04.014
更新日期:2017-05-04 00:00:00
abstract::Membrane scission is essential for intracellular trafficking. While BAR domain proteins such as endophilin have been reported in dynamin-independent scission of tubular membrane necks, the cutting mechanism has yet to be deciphered. Here, we combine a theoretical model, in vitro, and in vivo experiments revealing how ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.05.047
更新日期:2017-06-29 00:00:00
abstract::The p53 tumor-suppressor protein prevents cancer development through various mechanisms, including the induction of cell-cycle arrest, apoptosis, and the maintenance of genome stability. We have identified a p53-inducible gene named TIGAR (TP53-induced glycolysis and apoptosis regulator). TIGAR expression lowered fruc...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.05.036
更新日期:2006-07-14 00:00:00
abstract::The chaperonin GroEL binds nonnative proteins too large to fit inside the productive GroEL-GroES cis cavity, but whether and how it assists their folding has remained unanswered. We have examined yeast mitochondrial aconitase, an 82 kDa monomeric Fe(4)S(4) cluster-containing enzyme, observed to aggregate in chaperonin...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00523-2
更新日期:2001-10-19 00:00:00
abstract::In embryonal carcinoma (EC) cells retinoic acid (RA) strongly induces transcription from the RA receptor beta 2 (RAR beta 2) promoter through an RA response element (RARE) located in close proximity to the TATA box. Here we demonstrate that recombinant human TATA box-binding protein, hTFIID, and RAR functionally coope...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90443-g
更新日期:1992-05-01 00:00:00
abstract::STAT6 plays a prominent role in adaptive immunity by transducing signals from extracellular cytokines. We now show that STAT6 is required for innate immune signaling in response to virus infection. Viruses or cytoplasmic nucleic acids trigger STING (also named MITA/ERIS) to recruit STAT6 to the endoplasmic reticulum, ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.09.022
更新日期:2011-10-14 00:00:00
abstract::Hedgehog (Hh) proteins play diverse organizing roles in development by regulating gene expression in responding cells. The Gli homolog Cubitus interruptus (Ci) is involved in controlling the transcription of Hh target genes. A repressor form of Ci arises in the absence of Hh signaling by proteolytic cleavage of intact...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80592-9
更新日期:1999-03-19 00:00:00
abstract::Neural progenitors generate distinct cell types at different stages, but the mechanisms controlling these temporal transitions are poorly understood. In the Drosophila CNS, a cascade of transcription factors, the "temporal gene cascade," has been identified that acts to alter progenitor competence over time. However, ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.10.032
更新日期:2009-11-25 00:00:00
abstract::A recent report proposed a function of the ubiquitin conjugation factors Rad6 and Rad18 comparable to the bacterial SOS response, controlling damage-induced transcriptional activation and contributing to checkpoint signaling. The relevant ubiquitylation target was identified as budding yeast Rad17, a subunit of the PC...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2010.04.039
更新日期:2010-06-11 00:00:00
abstract::DNA polymerase (pol) β is a model polymerase involved in gap-filling DNA synthesis utilizing two metals to facilitate nucleotidyl transfer. Previous structural studies have trapped catalytic intermediates by utilizing substrate analogs (dideoxy-terminated primer or nonhydrolysable incoming nucleotide). To identify add...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.05.048
更新日期:2013-07-03 00:00:00
abstract::The BALC/c mouse has two nonallelic beta-globin genes that appear to reside on two different Eco R1 fragments of genomic DNA. We have already cloned one of these fragments and shown that the gene encoded within it is interrupted by at least one large intervening sequence of DNA. We have now cloned and characterized th...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90110-1
更新日期:1978-06-01 00:00:00
abstract::Methylation of arginine residues within histone H3 has been linked to active transcription. This modification appears on the estrogen-regulated pS2 promoter when the CARM1 methyltransferase is recruited during transcriptional activation. Here we describe a process, deimination, that converts histone arginine to citrul...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2004.08.020
更新日期:2004-09-03 00:00:00
abstract::The pandemic has impacted every scientist differently. Many negative impacts are frequently discussed. Here we highlight unexpected positives that we have found and hope will persist: improved access to experts; deeper and broader human engagement among colleagues, collaborators, and competitors; and significant democ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.09.027
更新日期:2020-10-01 00:00:00
abstract::Following TGFbeta receptor-mediated phosphorylation and association with Smad4, Smad2 moves into the nucleus, binds to target promoters in association with DNA-binding cofactors, and recruits coactivators such as p300/CBP to activate transcription. We identified the homeodomain protein TGIF as a Smad2-binding protein ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80712-6
更新日期:1999-04-02 00:00:00
abstract::Cis-regulatory elements (CREs) are commonly recognized by correlative chromatin features, yet the molecular composition of the vast majority of CREs in chromatin remains unknown. Here, we describe a CRISPR affinity purification in situ of regulatory elements (CAPTURE) approach to unbiasedly identify locus-specific chr...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.08.003
更新日期:2017-08-24 00:00:00
abstract::Phosphoinositide 3 kinase (PI3K) is a key signaling enzyme implicated in receptor-stimulated mitogenesis, oxidative bursting in neutrophils, membrane ruffling, and glucose uptake. A PI3K has already been purified, cloned, and shown to be regulated by receptors that act via tyrosine kinase-dependent regulatory mechanis...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90237-2
更新日期:1994-04-08 00:00:00
abstract::We have determined the complete 9202 nucleotide sequence of the visna lentivirus. The deduced genetic organization most closely resembles that of the AIDS retrovirus in that there is a novel central region separating pol and env. Moreover, there is a close phylogenetic relationship between the conserved reverse transc...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(85)80132-x
更新日期:1985-08-01 00:00:00
abstract::Adult skeletal muscle generates force in a controlled and directed manner through the contraction of highly specialized, postmitotic, multinucleated myofibers. Life-long muscle function relies on maintenance and regeneration of myofibers through a highly regulated process beginning with activation of normally quiescen...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2005.08.021
更新日期:2005-09-09 00:00:00
abstract::Conversion of glial cells into functional neurons represents a potential therapeutic approach for replenishing neuronal loss associated with neurodegenerative diseases and brain injury. Previous attempts in this area using expression of transcription factors were hindered by the low conversion efficiency and failure o...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.03.024
更新日期:2020-04-30 00:00:00
abstract::In order to identify nucleotide sequences required for efficient and accurate polyadenylation of mRNA precursors, we have constructed a series of mutations in the X. laevis beta 1-globin gene and analyzed transcripts produced upon microinjection into Xenopus oocytes. Small deletion and linker replacement mutations, wh...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90743-9
更新日期:1986-07-18 00:00:00
abstract::DMC1 is a new meiosis-specific yeast gene. Dmc1 protein is structurally similar to bacterial RecA proteins. dmc1 mutants are defective in reciprocal recombination, accumulate double-strand break (DSB) recombination intermediates, fail to form normal synaptonemal complex (SC), and arrest late in meiotic prophase. dmc1 ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90446-j
更新日期:1992-05-01 00:00:00
abstract::The primer transcript of plasmid ColE1 extends beyond the replication origin in either of two different modes. It does or does not form a hybrid with the template DNA. When a stretch of 20 deoxyadenosine residues is inserted into the template strand downstream of the origin, more than 90% of hybridized transcripts and...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90131-0
更新日期:1987-11-20 00:00:00
abstract::Molecular codes, like postal zip codes, are generally considered a robust way to ensure the specificity of neuronal target selection. However, a code capable of unambiguously generating complex neural circuits is difficult to conceive. Here, we re-examine the notion of molecular codes in the light of developmental alg...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2015.09.031
更新日期:2015-10-08 00:00:00
abstract::The MYC oncoproteins are thought to stimulate tumor cell growth and proliferation through amplification of gene transcription, a mechanism that has thwarted most efforts to inhibit MYC function as potential cancer therapy. Using a covalent inhibitor of cyclin-dependent kinase 7 (CDK7) to disrupt the transcription of a...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.10.024
更新日期:2014-11-20 00:00:00
abstract::Dysfunctional mitochondria accumulate in many human diseases. Accordingly, mitophagy, which removes these mitochondria through lysosomal degradation, is attracting broad attention. Due to uncertainties in the operational principles of conventional mitophagy probes, however, the specificity and quantitativeness of thei...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.04.025
更新日期:2020-05-28 00:00:00