Abstract：
:A mechanistic description of metazoan transcription is essential for understanding the molecular processes that govern cellular decisions. To provide structural insights into the DNA recognition step of transcription initiation, we used single-particle electron microscopy (EM) to visualize human TFIID with promoter DNA. This analysis revealed that TFIID coexists in two predominant and distinct structural states that differ by a 100 Å translocation of TFIID's lobe A. The transition between these structural states is modulated by TFIIA, as the presence of TFIIA and promoter DNA facilitates the formation of a rearranged state of TFIID that enables promoter recognition and binding. DNA labeling and footprinting, together with cryo-EM studies, were used to map the locations of TATA, Initiator (Inr), motif ten element (MTE), and downstream core promoter element (DPE) promoter motifs within the TFIID-TFIIA-DNA structure. The existence of two structurally and functionally distinct forms of TFIID suggests that the different conformers may serve as specific targets for the action of regulatory factors.
journal_name
Celljournal_title
Cellauthors
Cianfrocco MA,Kassavetis GA,Grob P,Fang J,Juven-Gershon T,Kadonaga JT,Nogales Edoi
10.1016/j.cell.2012.12.005subject
Has Abstractpub_date
2013-01-17 00:00:00pages
120-31issue
1-2eissn
0092-8674issn
1097-4172pii
S0092-8674(12)01492-4journal_volume
152pub_type
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