Abstract:
:sigma(70) subunit is thought to be released from the core RNA polymerase (RNAP) upon the transition from initiation to elongation or shortly afterward. Here, we identify a population of RNAP from E. coli that retains sigma(70) throughout elongation. The relative amount of this population appears to depend on cellular growth and reaches its maximum during the stationary phase. The proportion of sigma(70)-retaining elongation complexes (EC-sigma(70)) is invariant with various promoters or distances from the transcription start site. EC-sigma(70) responds to pauses, intrinsic terminators, and the elongation factor NusA similarly to EC without sigma(70). However, EC-sigma(70) has a substantially higher ability to support multiple rounds of transcription at certain promoters, suggesting its profound role in gene expression and regulation in bacteria.
journal_name
Celljournal_title
Cellauthors
Bar-Nahum G,Nudler Edoi
10.1016/s0092-8674(01)00461-5subject
Has Abstractpub_date
2001-08-24 00:00:00pages
443-51issue
4eissn
0092-8674issn
1097-4172pii
S0092-8674(01)00461-5journal_volume
106pub_type
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