A role for recombination junctions in the segregation of mitochondrial DNA in yeast.


:In S. cerevisiae, mitochondrial DNA (mtDNA) molecules, in spite of their high copy number, segregate as if there were a small number of heritable units. The rapid segregation of mitochondrial genomes can be analyzed using mtDNA deletion variants. These small, amplified genomes segregate preferentially from mixed zygotes relative to wild-type mtDNA. This segregation advantage is abolished by mutations in a gene, MGT1, that encodes a recombination junction-resolving enzyme. We show here that resolvase deficiency causes a larger proportion of molecules to be linked together by recombination junctions, resulting in the aggregation of mtDNA into a small number of cytological structures. This change in mtDNA structure can account for the increased mitotic loss of mtDNA and the altered pattern of mtDNA segregation from zygotes. We propose that the level of unresolved recombination junctions influences the number of heritable units of mtDNA.






Lockshon D,Zweifel SG,Freeman-Cook LL,Lorimer HE,Brewer BJ,Fangman WL




Has Abstract


1995-06-16 00:00:00
















  • Organ-level quorum sensing directs regeneration in hair stem cell populations.

    abstract::Coordinated organ behavior is crucial for an effective response to environmental stimuli. By studying regeneration of hair follicles in response to patterned hair plucking, we demonstrate that organ-level quorum sensing allows coordinated responses to skin injury. Plucking hair at different densities leads to a regene...


    pub_type: 杂志文章


    authors: Chen CC,Wang L,Plikus MV,Jiang TX,Murray PJ,Ramos R,Guerrero-Juarez CF,Hughes MW,Lee OK,Shi S,Widelitz RB,Lander AD,Chuong CM

    更新日期:2015-04-09 00:00:00

  • Ectopic expression of the floral homeotic gene AGAMOUS in transgenic Arabidopsis plants alters floral organ identity.

    abstract::The Arabidopsis floral homeotic gene AGAMOUS (AG) is required for development of the reproductive organs (stamens and carpels). In ag mutants, the loss of AG function leads to the conversion of these organs to the perianth organs (petals and sepals). In contrast, mutations in another floral homeotic gene, APETALA2 (AP...


    pub_type: 杂志文章


    authors: Mizukami Y,Ma H

    更新日期:1992-10-02 00:00:00

  • Blimp1 defines a progenitor population that governs cellular input to the sebaceous gland.

    abstract::Epidermal lineage commitment occurs when multipotent stem cells are specified to three lineages: the epidermis, the hair follicle, and the sebaceous gland (SG). How and when a lineage becomes specified remains unknown. Here, we report the existence of a population of unipotent progenitor cells that reside in the SG an...


    pub_type: 杂志文章


    authors: Horsley V,O'Carroll D,Tooze R,Ohinata Y,Saitou M,Obukhanych T,Nussenzweig M,Tarakhovsky A,Fuchs E

    更新日期:2006-08-11 00:00:00

  • HNRNPA2B1 Is a Mediator of m(6)A-Dependent Nuclear RNA Processing Events.

    abstract::N(6)-methyladenosine (m(6)A) is the most abundant internal modification of messenger RNA. While the presence of m(6)A on transcripts can impact nuclear RNA fates, a reader of this mark that mediates processing of nuclear transcripts has not been identified. We find that the RNA-binding protein HNRNPA2B1 binds m(6)A-be...


    pub_type: 杂志文章


    authors: Alarcón CR,Goodarzi H,Lee H,Liu X,Tavazoie S,Tavazoie SF

    更新日期:2015-09-10 00:00:00

  • IKKalpha at the crossroads of inflammation and metastasis.

    abstract::The development of solid tumors is regulated by dynamic interactions between evolving neoplastic cells and their microenvironment. Luo et al. (2007) recently demonstrated that tumor-infiltrating immune cells expressing RANKL induce activation and nuclear localization of IKKalpha in prostatic epithelial tumor cells. Th...


    pub_type: 杂志文章


    authors: Affara NI,Coussens LM

    更新日期:2007-04-06 00:00:00

  • Cell surface expression of an in vitro recombinant class II/class I major histocompatibility complex gene product.

    abstract::Chimeric histocompatibility genes encoding the amino-terminal (beta 1) domain of the class II Ak beta polypeptide and the carboxy-terminal (C2, transmembrane, and intracytoplasmic) domains of either the class I H-2Ld or H-2Dd molecules were stably introduced into mouse L cells. Although both were transcribed, only 5' ...


    pub_type: 杂志文章


    authors: McCluskey J,Germain RN,Margulies DH

    更新日期:1985-02-01 00:00:00

  • The in vitro transcription of the 7SK RNA gene by RNA polymerase III is dependent only on the presence of an upstream promoter.

    abstract::Deletion analysis was carried out on the human 7SK RNA gene to map regions essential for in vitro transcription by RNA polymerase III. The sequence promoting transcription is located between 37 and 3 bp upstream of the 7SK RNA coding region. RNA polymerase III transcription of adjacent plasmid sequences can be directe...


    pub_type: 杂志文章


    authors: Murphy S,Di Liegro C,Melli M

    更新日期:1987-10-09 00:00:00

  • Effects of androgens on the complexity of poly(A) RNA from rat prostate.

    abstract::Poly(A)-containing RNA, isolated from rat ventral prostate, has been analyzed for its base sequence complexity. The kinetics of hybridization of total cellular poly(A)-containing RNA with its complementary DNA (cDNA) from normal and castrated animals are markedly different. RNA from normal animals consists of three ab...


    pub_type: 杂志文章


    authors: Parker MG,Mainwaring WI

    更新日期:1977-10-01 00:00:00

  • Chemical Proteomics Identifies Druggable Vulnerabilities in a Genetically Defined Cancer.

    abstract::The transcription factor NRF2 is a master regulator of the cellular antioxidant response, and it is often genetically activated in non-small-cell lung cancers (NSCLCs) by, for instance, mutations in the negative regulator KEAP1. While direct pharmacological inhibition of NRF2 has proven challenging, its aberrant activ...


    pub_type: 杂志文章


    authors: Bar-Peled L,Kemper EK,Suciu RM,Vinogradova EV,Backus KM,Horning BD,Paul TA,Ichu TA,Svensson RU,Olucha J,Chang MW,Kok BP,Zhu Z,Ihle NT,Dix MM,Jiang P,Hayward MM,Saez E,Shaw RJ,Cravatt BF

    更新日期:2017-10-19 00:00:00

  • Accelerated evolution of nervous system genes in the origin of Homo sapiens.

    abstract::Human evolution is characterized by a dramatic increase in brain size and complexity. To probe its genetic basis, we examined the evolution of genes involved in diverse aspects of nervous system biology. We found that these genes display significantly higher rates of protein evolution in primates than in rodents. Impo...


    pub_type: 杂志文章


    authors: Dorus S,Vallender EJ,Evans PD,Anderson JR,Gilbert SL,Mahowald M,Wyckoff GJ,Malcom CM,Lahn BT

    更新日期:2004-12-29 00:00:00

  • Site-specific base substitution and deletion mutations that enhance or suppress transcription of the SV40 major late RNA.

    abstract::Transcriptional analysis of SV40 late promoter mutants indicates that the DNA sequence 5'-GGTACCTAACC-3' (map positions 294-304) is important in the control of SV40 late RNA expression. C to T base substitutions at map positions 298, 299 and 304 increased initiation of late RNA synthesis at map position 325 five to te...


    pub_type: 杂志文章


    authors: Brady J,Radonovich M,Vodkin M,Natarajan V,Thoren M,Das G,Janik J,Salzman NP

    更新日期:1982-12-01 00:00:00

  • Antigen Identification for Orphan T Cell Receptors Expressed on Tumor-Infiltrating Lymphocytes.

    abstract::The immune system can mount T cell responses against tumors; however, the antigen specificities of tumor-infiltrating lymphocytes (TILs) are not well understood. We used yeast-display libraries of peptide-human leukocyte antigen (pHLA) to screen for antigens of "orphan" T cell receptors (TCRs) expressed on TILs from h...


    pub_type: 杂志文章


    authors: Gee MH,Han A,Lofgren SM,Beausang JF,Mendoza JL,Birnbaum ME,Bethune MT,Fischer S,Yang X,Gomez-Eerland R,Bingham DB,Sibener LV,Fernandes RA,Velasco A,Baltimore D,Schumacher TN,Khatri P,Quake SR,Davis MM,Garcia KC

    更新日期:2018-01-25 00:00:00

  • Crystal structure of a UBP-family deubiquitinating enzyme in isolation and in complex with ubiquitin aldehyde.

    abstract::The ubiquitin-specific processing protease (UBP) family of deubiquitinating enzymes plays an essential role in numerous cellular processes. HAUSP, a representative UBP, specifically deubiquitinates and hence stabilizes the tumor suppressor protein p53. Here, we report the crystal structures of the 40 kDa catalytic cor...


    pub_type: 杂志文章


    authors: Hu M,Li P,Li M,Li W,Yao T,Wu JW,Gu W,Cohen RE,Shi Y

    更新日期:2002-12-27 00:00:00

  • Precise identification of individual promoters for transcription of each strand of human mitochondrial DNA.

    abstract::The major site of in vivo transcriptional initiation for both heavy and light strands of human mitochondrial DNA is the displacement-loop region. Transcripts synthesized in vitro by human mitochondrial RNA polymerase were mapped to the nucleotide level and have identical 5' end map positions to those reported for in v...


    pub_type: 杂志文章


    authors: Chang DD,Clayton DA

    更新日期:1984-03-01 00:00:00

  • Cell interactions coordinate the development of the C. elegans egg-laying system.

    abstract::Egg laying by the nematode Caenorhabditis elegans requires the functioning of the vulva, the gonad, the egg-laying muscles, and the two HSN neurons, which innervate these muscles. By analyzing a newly isolated mutant (dig-1) that displaces the gonad, we discovered that cell interactions coordinate the spatial relation...


    pub_type: 杂志文章


    authors: Thomas JH,Stern MJ,Horvitz HR

    更新日期:1990-09-21 00:00:00

  • Crystal structure of the glucocorticoid receptor ligand binding domain reveals a novel mode of receptor dimerization and coactivator recognition.

    abstract::Transcriptional regulation by the glucocorticoid receptor (GR) is mediated by hormone binding, receptor dimerization, and coactivator recruitment. Here, we report the crystal structure of the human GR ligand binding domain (LBD) bound to dexamethasone and a coactivator motif derived from the transcriptional intermedia...


    pub_type: 杂志文章


    authors: Bledsoe RK,Montana VG,Stanley TB,Delves CJ,Apolito CJ,McKee DD,Consler TG,Parks DJ,Stewart EL,Willson TM,Lambert MH,Moore JT,Pearce KH,Xu HE

    更新日期:2002-07-12 00:00:00

  • TLP1: a gene encoding a protein component of mammalian telomerase is a novel member of WD repeats family.

    abstract::We have cloned and characterized the rat telomerase protein component 1 gene (TLP1), which is related to the gene for Tetrahymena p80. The cDNA encodes a 2629 amino acid sequence and produces the TLP1 proteins p240 and p230. The anti-TLP1 antibody specifically immunoprecipitated the telomerase activity. Moreover, p240...


    pub_type: 杂志文章


    authors: Nakayama J,Saito M,Nakamura H,Matsuura A,Ishikawa F

    更新日期:1997-03-21 00:00:00

  • Mesenchymal to epithelial conversion in rat metanephros is induced by LIF.

    abstract::Inductive signals cause conversion of mesenchyme into epithelia during the formation of many organs. Yet a century of study has not revealed the inducing molecules. Using a standard model of induction, we found that ureteric bud cells secrete factors that convert kidney mesenchyme to epithelia that, remarkably, then f...


    pub_type: 杂志文章


    authors: Barasch J,Yang J,Ware CB,Taga T,Yoshida K,Erdjument-Bromage H,Tempst P,Parravicini E,Malach S,Aranoff T,Oliver JA

    更新日期:1999-11-12 00:00:00

  • High-Throughput Investigation of Diverse Junction Elements in RNA Tertiary Folding.

    abstract::RNAs fold into defined tertiary structures to function in critical biological processes. While quantitative models can predict RNA secondary structure stability, we are still unable to predict the thermodynamic stability of RNA tertiary structure. Here, we probe conformational preferences of diverse RNA two-way juncti...


    pub_type: 杂志文章


    authors: Denny SK,Bisaria N,Yesselman JD,Das R,Herschlag D,Greenleaf WJ

    更新日期:2018-07-12 00:00:00

  • Hedgehog controls limb development by regulating the activities of distinct transcriptional activator and repressor forms of Cubitus interruptus.

    abstract::Hedgehog (Hh) proteins play diverse organizing roles in development by regulating gene expression in responding cells. The Gli homolog Cubitus interruptus (Ci) is involved in controlling the transcription of Hh target genes. A repressor form of Ci arises in the absence of Hh signaling by proteolytic cleavage of intact...


    pub_type: 杂志文章


    authors: Méthot N,Basler K

    更新日期:1999-03-19 00:00:00

  • Ubiquitin dependence of selective protein degradation demonstrated in the mammalian cell cycle mutant ts85.

    abstract::We have shown that covalent conjugation of ubiquitin to proteins is temperature-sensitive in the mouse cell cycle mutant ts85 due to a specifically thermolabile ubiquitin-activating enzyme (accompanying paper). We show here that degradation of short-lived proteins is also temperature sensitive in ts85 , in contrast to...


    pub_type: 杂志文章


    authors: Ciechanover A,Finley D,Varshavsky A

    更新日期:1984-05-01 00:00:00

  • Enhancement of cellular src gene product associated tyrosyl kinase activity following polyoma virus infection and transformation.

    abstract::We examine the interaction between polyoma-virus-encoded middle tumor antigen and the cellular src gene product, pp60c-src, using a series of monoclonal antibodies that recognize mammalian pp60c-src. Our results show that infection of mouse cells with transformation-competent strains of polyoma virus results in the st...


    pub_type: 杂志文章


    authors: Bolen JB,Thiele CJ,Israel MA,Yonemoto W,Lipsich LA,Brugge JS

    更新日期:1984-10-01 00:00:00

  • The sqt-1 gene of C. elegans encodes a collagen critical for organismal morphogenesis.

    abstract::Different mutations in the sqt-1 gene of C. elegans can lengthen, shorten, or helically twist the entire animal. We have cloned the sqt-1 gene and have shown that it encodes a collagen. sqt-1 was localized to a 35 kb region of DNA by physical mapping of chromosomal deficiencies. A transposon (Tc1)-induced mutation of ...


    pub_type: 杂志文章


    authors: Kramer JM,Johnson JJ,Edgar RS,Basch C,Roberts S

    更新日期:1988-11-18 00:00:00

  • Characterization of the bovine papilloma virus plasmid maintenance sequences.

    abstract::Bovine Papilloma Virus (BPV-1) establishes itself as a multicopy nuclear plasmid in somatic mammalian cells in culture. We report here that two discontinuous regions within the viral genome can independently support extrachromosomal replication of the Tn5 neomycinr gene in cells that provide viral factors in trans. Th...


    pub_type: 杂志文章


    authors: Lusky M,Botchan MR

    更新日期:1984-02-01 00:00:00

  • Probing the Diversity of T Cell Dysfunction in Cancer.

    abstract::T cell dysfunction in cancer comes in many forms, with two new varieties reported in this issue. Daley et al. find that T cells expressing γδ T cell receptors (TCR) promote pancreatic tumor growth by inhibiting activation of T cells with conventional TCRs. Singer et al. characterize dysfunctional tumor infiltrating ly...


    pub_type: 评论,杂志文章


    authors: Sowell RT,Kaech SM

    更新日期:2016-09-08 00:00:00

  • Axonal remodeling and synaptic differentiation in the cerebellum is regulated by WNT-7a signaling.

    abstract::Synapse formation requires changes in cell morphology and the upregulation and localization of synaptic proteins. In the cerebellum, mossy fibers undergo extensive remodeling as they contact several granule cells and form complex, multisynaptic glomerular rosettes. Here we show that granule cells secrete factors that ...


    pub_type: 杂志文章


    authors: Hall AC,Lucas FR,Salinas PC

    更新日期:2000-03-03 00:00:00

  • RNA polymerase interactions with the upstream region of the E. coli tyrT promoter.

    abstract::The rate of in vivo transcription from the E. coli tRNA and rRNA promoters depends on both cellular growth rate and aminoacid availability. To investigate the molecular mechanisms involved we determined the extent of interaction of RNA polymerase with the promoter of the tyrT stable RNA gene. We show that the enzyme c...


    pub_type: 杂志文章


    authors: Travers AA,Lamond AI,Mace HA,Berman ML

    更新日期:1983-11-01 00:00:00

  • Monoculture breeds poor social skills.

    abstract::Two studies from Jarosz et al. describe how [GAR(+)], a protein-based epigenetic determinant found mainly in wild yeast strains, can be activated by microbial cross-kingdom communication. With the aid of genetically and ecologically diverse bacteria, yeast can override an ancient regulatory mechanism of glucose repres...


    pub_type: 评论,杂志文章


    authors: Staniforth GL,Tuite MF

    更新日期:2014-08-28 00:00:00

  • In and out of the replication factory.

    abstract::In this issue of Cell, use live-fluorescence microscopy to monitor individual genomic loci as they replicate in budding yeast. They confirm that DNA is recruited to replication factories and show that sister replication forks initiated from the same origin are held together within a single replication factory. ...


    pub_type: 评论,杂志文章


    authors: Meister P,Taddei A,Gasser SM

    更新日期:2006-06-30 00:00:00

  • Functional analysis of a growth factor-responsive transcription factor complex.

    abstract::Serum response factor (SRF) forms a ternary complex at the c-fos serum response element (SRE) with an accessory factor, Elk-1. We constructed altered-binding specificity derivatives of SRF and Elk-1 that form a ternary complex at a mutated, inactive SRE; like Elk-1, the Elk-1 variant only binds its target as part of a...


    pub_type: 杂志文章


    authors: Hill CS,Marais R,John S,Wynne J,Dalton S,Treisman R

    更新日期:1993-04-23 00:00:00