A role for recombination junctions in the segregation of mitochondrial DNA in yeast.

abstract：:An adaptive variant of the human Ectodysplasin receptor, EDARV370A, is one of the strongest candidates of recent positive selection from genome-wide scans. We have modeled EDAR370A in mice and characterized its phenotype and evolutionary origins in humans. Our computational analysis suggests the allele arose in centra...

journal_title：Cell

authors： Kamberov YG,Wang S,Tan J,Gerbault P,Wark A,Tan L,Yang Y,Li S,Tang K,Chen H,Powell A,Itan Y,Fuller D,Lohmueller J,Mao J,Schachar A,Paymer M,Hostetter E,Byrne E,Burnett M,McMahon AP,Thomas MG,Lieberman DE,Jin

更新日期：2013-02-14 00:00:00

abstract：:We describe genetic and physical characterization of rearrangements of chromosome III which result in changes of cell type in S. cerevisiae. Two types of rearrangements were obtained as rare events which caused a change at the locus controlling cell type, MAT, associated with a recessive lethal mutation, in one case f...

journal_title：Cell

authors： Strathern JN,Newlon CS,Herskowitz I,Hicks JB

更新日期：1979-10-01 00:00:00

abstract：:In this study, we have located the sites of transcription initiation and termination on a cloned fragment of ribosomal DNA from X. laevis, and have sequenced the surrounding nucleotides. As reported previously (Reeder, Sollner-Webb and Wahn, 1977), about 25% of the 40S rRNA precursor molecules isolated from oocytes ha...

journal_title：Cell

authors： Sollner-Webb B,Reeder RH

更新日期：1979-10-01 00:00:00

abstract：:The growth of lymphatic vessels (lymphangiogenesis) is actively involved in a number of pathological processes including tissue inflammation and tumor dissemination but is insufficient in patients suffering from lymphedema, a debilitating condition characterized by chronic tissue edema and impaired immunity. The recen...

journal_title：Cell

authors： Tammela T,Alitalo K

更新日期：2010-02-19 00:00:00

abstract：:The commitment complex is the first defined step in the yeast (S. cerevisiae) splicing pathway. It contains U1 snRNP as well as Mud2p, which resembles human U2AF65. In a genetic screen, we identified the yeast gene MSL-5, which is a novel commitment complex component. Genetic and biochemical criteria indicate a direct...

journal_title：Cell

authors： Abovich N,Rosbash M

更新日期：1997-05-02 00:00:00

abstract：:The S. cerevisiae gene MCR1 encodes two mitochondrial isoforms of NADH-cytochrome b5 reductase. The primary translation product has an amino-terminal matrix-targeting signal, followed by a stretch of 21 uncharged amino acids. This precursor protein is inserted into the outer membrane, but only about one-third of the m...

journal_title：Cell

authors： Hahne K,Haucke V,Ramage L,Schatz G

更新日期：1994-12-02 00:00:00

abstract：:A plasmid containing the major late promoter was assembled into nucleosomes in a Xenopus oocyte extract, isolated by gel filtration, and found to be refractory to transcription initiation in vitro. However, exposure of the promoter to HeLa nuclear extract or to a mixture of isolated transcription factors prior to nucl...

journal_title：Cell

authors： Workman JL,Roeder RG

更新日期：1987-11-20 00:00:00

abstract：:Nociceptin and its receptor are widely distributed throughout the brain in regions associated with reward behavior, yet how and when they act is unknown. Here, we dissected the role of a nociceptin peptide circuit in reward seeking. We generated a prepronociceptin (Pnoc)-Cre mouse line that revealed a unique subpopula...

journal_title：Cell

authors： Parker KE,Pedersen CE,Gomez AM,Spangler SM,Walicki MC,Feng SY,Stewart SL,Otis JM,Al-Hasani R,McCall JG,Sakers K,Bhatti DL,Copits BA,Gereau RW,Jhou T,Kash TJ,Dougherty JD,Stuber GD,Bruchas MR

更新日期：2019-07-25 00:00:00

abstract：:The Escherichia coli relBE operon encodes a toxin-antitoxin pair, RelE-RelB. RelB can reverse inhibition of protein synthesis by RelE in vivo. We have found that although RelE does not degrade free RNA, it cleaves mRNA in the ribosomal A site with high codon specificity. Among stop codons UAG is cleaved with fast, UAA...

journal_title：Cell

authors： Pedersen K,Zavialov AV,Pavlov MY,Elf J,Gerdes K,Ehrenberg M

更新日期：2003-01-10 00:00:00

abstract：:The differentiation of follicular dendritic cells (FDC) is essential to the remarkable microanatomic plasticity of lymphoid follicles. Here we show that FDC arise from ubiquitous perivascular precursors (preFDC) expressing platelet-derived growth factor receptor β (PDGFRβ). PDGFRβ-Cre-driven reporter gene recombinatio...

journal_title：Cell

authors： Krautler NJ,Kana V,Kranich J,Tian Y,Perera D,Lemm D,Schwarz P,Armulik A,Browning JL,Tallquist M,Buch T,Oliveira-Martins JB,Zhu C,Hermann M,Wagner U,Brink R,Heikenwalder M,Aguzzi A

更新日期：2012-07-06 00:00:00

abstract：:Cyclic dinucleotides (CDNs) play central roles in bacterial pathogenesis and innate immunity. The mammalian enzyme cGAS synthesizes a unique cyclic dinucleotide (cGAMP) containing a 2'-5' phosphodiester linkage essential for optimal immune stimulation, but the molecular basis for linkage specificity is unknown. Here, ...

journal_title：Cell

authors： Kranzusch PJ,Lee ASY,Wilson SC,Solovykh MS,Vance RE,Berger JM,Doudna JA

更新日期：2014-08-28 00:00:00

abstract：:32P-labeled cowpea mosaic virus (CPMV) RNAs were analyzed for their 5' termini by three different fingerprinting standard procedures. These analyses showed unequivocally that CPMV RNAs lack a structure like m7GpppN ... at their 5' termini. In addition, neither mono-, di- or triphosphorylated [(p)(p)pN ....] termini we...

journal_title：Cell

authors： Klootwijk J,Klein I,Zabel P,van Kammen A

更新日期：1977-05-01 00:00:00

abstract：:Human cerebral cortex size and complexity has increased greatly during evolution. While increased progenitor diversity and enhanced proliferative potential play important roles in human neurogenesis and gray matter expansion, the mechanisms of human oligodendrogenesis and white matter expansion remain largely unknown....

journal_title：Cell

authors： Huang W,Bhaduri A,Velmeshev D,Wang S,Wang L,Rottkamp CA,Alvarez-Buylla A,Rowitch DH,Kriegstein AR

更新日期：2020-08-06 00:00:00

abstract：:The protein Par-4 acts in the cytoplasm to trigger cell death signaling via caspase activation and the mitochondrial release of cytochrome c. Burikhanov et al. (2009) now provide surprising evidence that Par-4 can also promote apoptosis from outside the cell, after its secretion in response to endoplasmic reticulum st...

journal_title：Cell

authors： Hart LS,El-Deiry WS

更新日期：2009-07-23 00:00:00

abstract：:Nuclear exclusion of the forkhead transcription factor FOXO3a by protein kinase Akt contributes to cell survival. We investigated the pathological relationship between phosphoylated-Akt (Akt-p) and FOXO3a in primary tumors. Surprisingly, FOXO3a was found to be excluded from the nuclei of some tumors lacking Akt-p, sug...

journal_title：Cell

authors： Hu MC,Lee DF,Xia W,Golfman LS,Ou-Yang F,Yang JY,Zou Y,Bao S,Hanada N,Saso H,Kobayashi R,Hung MC

更新日期：2004-04-16 00:00:00

abstract：:We have isolated five clones containing human histone genes from a human genomic DNA library, using a cloned X. laevis histone H4 cDNA probe (pX1ch4). Each genomic clone has been mapped and the locations of the histone genes have been determined by blot hybridization and hybridization-selection of human histone mRNA. ...

journal_title：Cell

authors： Heintz N,Zernik M,Roeder RG

更新日期：1981-06-01 00:00:00

abstract：:Nucleosomes help structure chromosomes by compacting DNA into fibers. To gain insight into how nucleosomes are arranged in vivo, we combined quantitative super-resolution nanoscopy with computer simulations to visualize and count nucleosomes along the chromatin fiber in single nuclei. Nucleosomes assembled in heteroge...

journal_title：Cell

authors： Ricci MA,Manzo C,García-Parajo MF,Lakadamyali M,Cosma MP

更新日期：2015-03-12 00:00:00

abstract：:In C. elegans, removing the germ cells extends life span by triggering the nuclear localization and activation of the DAF-16/FOXO transcription factor in the intestine. In this study, we identify and analyze genes required for germline removal to extend life span. We find that the reproductive system communicates with...

journal_title：Cell

authors： Berman JR,Kenyon C

更新日期：2006-03-10 00:00:00

abstract：:The role of electricity in biological systems was first appreciated through electrical stimulation experiments performed by Luigi Galvani in the 18th century. These pioneering experiments demonstrated that the behavior of living tissues is governed by the flow of electrochemical species-an insight that gave rise to th...

journal_title：Cell

authors： Lee DD,Prindle A,Liu J,Süel GM

更新日期：2017-06-29 00:00:00

abstract：:The state and organization of viral DNA sequences present in independently isolated rat cell lines transformed with SV40 were investigated using restriction-enzyme cleavage of the cellular DNA and blot hybridization with a viral probe. The transformed lines were established under conditions as identical as possible, e...

journal_title：Cell

authors： Mougneau E,Birg F,Rassoulzadegan M,Cuzin F

更新日期：1980-12-01 00:00:00

abstract：:Synaptotagmin is an abundant synaptic vesicle-associated protein proposed to be involved in calcium-mediated neurotransmitter release. Our molecular and genetic results demonstrate that, although synaptotagmin is required for the proper function of the presynaptic nerve terminal in C. elegans, some neurotransmitter re...

journal_title：Cell

authors： Nonet ML,Grundahl K,Meyer BJ,Rand JB

更新日期：1993-07-02 00:00:00

abstract：:Salmonella enterica invasion of host cells requires the reversible activation of the Rho-family GTPases Cdc42 and Rac1 by the bacterially encoded GEF SopE and the GAP SptP, which exert their function at different times during infection and are delivered into host cells by a type III secretion system. We found that Sop...

journal_title：Cell

authors： Kubori T,Galán JE

更新日期：2003-10-31 00:00:00

abstract：:We explored how the odor map in the Drosophila antennal lobe is represented in higher olfactory centers, the mushroom body and lateral horn. Systematic single-cell tracing of projection neurons (PNs) that send dendrites to specific glomeruli in the antennal lobe revealed their stereotypical axon branching patterns and...

journal_title：Cell

authors： Marin EC,Jefferis GS,Komiyama T,Zhu H,Luo L

更新日期：2002-04-19 00:00:00

abstract：:Sec7-related guanine nucleotide exchange factors (GEFs) initiate vesicle budding from the Golgi membrane surface by converting the GTPase ARF to a GTP-bound, membrane-associated form. Here we report the crystal structure of the catalytic Sec7 homology domain of Arno, a human GEF for ARF1, determined at 2.2 angstroms r...

journal_title：Cell

authors： Mossessova E,Gulbis JM,Goldberg J

更新日期：1998-02-06 00:00:00

abstract：:Rat liver interphase chromosomes have telomeres 20-100 kb in length. Micrococcal nuclease digestion of nuclei cleaves telomeres with a uniform 157 bp periodicity, producing soluble particles that sediment in sucrose gradients exactly like oligonucleosomes. The monomeric telomere particles comigrate with nucleosome cor...

journal_title：Cell

authors： Makarov VL,Lejnine S,Bedoyan J,Langmore JP

更新日期：1993-05-21 00:00:00

abstract：:In Saccharomyces cerevisiae, known histone acetylation sites regulating gene activity are located in the N-terminal tails protruding from the nucleosome core. We report lysine 56 in histone H3 as a novel acetylation site that is located in the globular domain, where it extends toward the DNA major groove at the entry-...

journal_title：Cell

authors： Xu F,Zhang K,Grunstein M

更新日期：2005-05-06 00:00:00

abstract：:In the SV40-transformed mouse embryo fibroblast cell line SVT2/S, genomic rearrangements involving the SV40 DNA and flanking host sequences were identified by Southern blot hybridization using viral DNA as probe. No rearrangements of SV40 DNA integrated into nonpermissive mouse cells have been previously described. Th...

journal_title：Cell

authors： Sager R,Anisowicz A,Howell N

更新日期：1981-01-01 00:00:00

abstract：:ETS transcription factors ETV2, FLI1, and ERG1 specify pluripotent stem cells into induced vascular endothelial cells (iVECs). However, iVECs are unstable and drift toward nonvascular cells. We show that human midgestation c-Kit(-) lineage-committed amniotic cells (ACs) can be reprogrammed into vascular endothelial ce...

journal_title：Cell

authors： Ginsberg M,James D,Ding BS,Nolan D,Geng F,Butler JM,Schachterle W,Pulijaal VR,Mathew S,Chasen ST,Xiang J,Rosenwaks Z,Shido K,Elemento O,Rabbany SY,Rafii S

更新日期：2012-10-26 00:00:00

abstract：:While developing an assay to measure the activity of the tat protein from human immunodeficiency virus 1 (HIV-1), we discovered that the purified protein could be taken up by cells growing in tissue culture and subsequently trans-activate the viral promoter. Trans-activation is dramatically increased by a variety of l...

journal_title：Cell

authors： Frankel AD,Pabo CO

更新日期：1988-12-23 00:00:00

abstract：:We have performed in vitro kinetic analyses of transcription from the 5S gene promoter in order to resolve the rate-limiting events which lead to accurate transcription of the 5S RNA gene. We demonstrate that a rate-limiting intermediate can be formed during an extended incubation prior to initiation of transcription....

journal_title：Cell

authors： Bieker JJ,Martin PL,Roeder RG

更新日期：1985-01-01 00:00:00