Abstract:
:What happens to organelles during mitosis, and how they are apportioned to each of the daughter cells, is not completely clear. We have devised a procedure to address whether Golgi membranes fuse with the Endoplasmic Reticulum (ER) during mitosis via the detection of interactions between ER and Golgi proteins. This procedure involves coexpressing an FKBP-tagged Golgi enzyme with an ER-retained protein fused to FRAP in COS cells. Since treatment with rapamycin induces a tight association between FKBP and FRAP, one would expect rapamycin to trap the FKBP-fused Golgi protein in the ER if it ever visits the ER during mitosis. However, after the doubly transfected cells progress through mitosis in the presence of rapamycin, we find the Golgi protein in the newly formed Golgi stacks and not in the ER. Based on these results, we conclude that Golgi membranes remain separate from the ER during mitosis in mammalian cells.
journal_name
Celljournal_title
Cellauthors
Pecot MY,Malhotra Vdoi
10.1016/s0092-8674(03)01068-7subject
Has Abstractpub_date
2004-01-09 00:00:00pages
99-107issue
1eissn
0092-8674issn
1097-4172pii
S0092867403010687journal_volume
116pub_type
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