Abstract:
:Four RNA products have been characterized from a soluble system that accurately splices purified substrate RNA. The labeled substrate RNA contained the first and second exons of the major late transcription unit of adenovirus 2 and a shortened form of the first intervening sequence. One of these RNA products was the excised intervening sequence which accumulated quantitatively with the level of splicing. This RNA species has an unusual structure and is modified at internal sites. A potential intermediate in the splicing pathway which is cleaved at the 5' splice site of the first exon has been isolated. This RNA species is also modified at sites within the first intervening sequence. These results suggest that the splicing of mRNA precursors may involve sites in the intervening sequence, cleavage at the 5' splice site, cleavage at the 3' splice site, and ligation of the two exons.
journal_name
Celljournal_title
Cellauthors
Grabowski PJ,Padgett RA,Sharp PAdoi
10.1016/0092-8674(84)90372-6subject
Has Abstractpub_date
1984-06-01 00:00:00pages
415-27issue
2eissn
0092-8674issn
1097-4172pii
0092-8674(84)90372-6journal_volume
37pub_type
杂志文章相关文献
CELL文献大全abstract::Cancer immunotherapy can induce long lasting responses in patients with metastatic cancers of a wide range of histologies. Broadening the clinical applicability of these treatments requires an improved understanding of the mechanisms limiting cancer immunotherapy. The interactions between the immune system and cancer ...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2017.01.017
更新日期:2017-02-09 00:00:00
abstract::The mitotic checkpoint ensures proper chromosome segregation by delaying anaphase until chromosomes are aligned on the spindle. Following prolonged spindle damage, however, cells eventually exit mitosis and undergo apoptosis. We show here that a murine homolog of the yeast mitotic checkpoint gene BUB1 localizes to the...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80255-x
更新日期:1997-05-30 00:00:00
abstract::The genetic dependencies of human cancers widely vary. Here, we catalog this heterogeneity and use it to identify functional gene interactions and genotype-dependent liabilities in cancer. By using genome-wide CRISPR-based screens, we generate a gene essentiality dataset across 14 human acute myeloid leukemia (AML) ce...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.01.013
更新日期:2017-02-23 00:00:00
abstract::The anaphase-promoting complex (APC), or cyclosome, is an E3 ubiquitin-protein ligase that collaborates with E2 ubiquitin-conjugating enzymes to assemble polyubiquitin chains on proteins important for cell-cycle progression. It remains unclear how the APC - or many other E3s - promotes the multiple distinct reactions ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.05.027
更新日期:2007-07-13 00:00:00
abstract::Autophagy is a catabolic process in which lysosomes degrade intracytoplasmic contents transported in double-membraned autophagosomes. Autophagosomes are formed by the elongation and fusion of phagophores, which can be derived from preautophagosomal structures coming from the plasma membrane and other sites like the en...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.06.023
更新日期:2011-07-22 00:00:00
abstract::The inhibitor of apoptosis (IAP) proteins suppress cell death by inhibiting the catalytic activity of caspases. Here we present the crystal structure of caspase-7 in complex with a potent inhibitory fragment from XIAP at 2.45 A resolution. An 18-residue XIAP peptide binds the catalytic groove of caspase-7, making exte...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00272-0
更新日期:2001-03-09 00:00:00
abstract::The functional consequences of glycan structural changes associated with cellular differentiation are ill defined. Herein, we investigate the role of glycan adducts to the O-glycosylated polypeptide stalk tethering the CD8alphabeta coreceptor to the thymocyte surface. We show that immature CD4(+)CD8(+) double-positive...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00577-3
更新日期:2001-11-16 00:00:00
abstract::Post-translational modifications of histone proteins and exchanges of histone variants of chromatin are central to the regulation of nearly all DNA-templated biological processes. However, the degree and variability of chromatin modifications in specific human immune cells remain largely unknown. Here, we employ a hig...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.03.079
更新日期:2018-05-31 00:00:00
abstract::Methylation of arginine residues within histone H3 has been linked to active transcription. This modification appears on the estrogen-regulated pS2 promoter when the CARM1 methyltransferase is recruited during transcriptional activation. Here we describe a process, deimination, that converts histone arginine to citrul...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2004.08.020
更新日期:2004-09-03 00:00:00
abstract::We have developed an in vitro system utilizing yeast cell-free extracts to catalyze recombination events between homologous plasmids containing different mutant alleles of the Tet or ARG4 genes. The reaction increased the frequency of Tcr or Arg+ transformants (recombinants) from 2 X 10(-6) to 1-3 X 10(-3). Linearizin...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90113-7
更新日期:1983-12-01 00:00:00
abstract::The "protective protein" is a glycoprotein that associates with lysosomal beta-galactosidase and neuraminidase and is deficient in the autosomal recessive disorder galactosialidosis. We have isolated the cDNA encoding human "protective protein". The clone recognizes a 2 kb mRNA in normal cells that is not evident in f...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(88)90999-3
更新日期:1988-09-09 00:00:00
abstract::Investigating the family tree of a tumor to identify its cellular origins is a daunting task. Liu et al. (2011) now use an elegant lineage tracing technique (MADM) to visualize glioma from its earliest stages. They show that mutations originally induced in neural stem cells lie dormant and only trigger malignant trans...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2011.06.047
更新日期:2011-07-22 00:00:00
abstract::Hybrid transducing phages were constructed in vitro that carry the galK gene fused to each of three ribosomal promoters: the promotor for an rRNA operon (rrnE); the promoter for the spec r protein operon and the promotor for the alpha r protein operon. We also constructed hybrid transducing phages that carry the IacZ ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90185-9
更新日期:1981-09-01 00:00:00
abstract::Fragile X syndrome results from the expansion of a CGG repeat that is normally interrupted by occasional AGG triplets. Linkage disequilibrium suggests that certain normal haplotypes may contribute unequally to the pool of fragile X chromosomes. Sequence analysis of normal alleles demonstrates haplotype-specific variat...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90134-1
更新日期:1994-06-17 00:00:00
abstract::Plants, compared to animals, exhibit an amazing adaptability and plasticity in their development. This is largely dependent on the ability of plants to form new organs, such as lateral roots, leaves, and flowers during postembryonic development. Organ primordia develop from founder cell populations into organs by coor...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00924-3
更新日期:2003-11-26 00:00:00
abstract::We wished to learn whether the genomes of strains of EMB isolated from patients with infectious mononucleosis are consistently distinguishable from those of strains from Burkitt's lymphoma. The genome of a new transforming strains (FF41) of EBV isolated from saliva of a patient with uncomplicated infectious mononucleo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90345-7
更新日期:1981-05-01 00:00:00
abstract::We have examined the molecular organization of a region of mouse chromosome 17 that allows homologous recombination between wild-type and t haplotype chromosomes across a large inversion. We have used a combination of genetic mapping of restriction fragment length polymorphisms, molecular characterization of cloned re...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90078-x
更新日期:1987-03-13 00:00:00
abstract::Neurons make networks of intercellular connections. Many other cells also send out long cellular extensions and "touch" other cells far away. The extensions are intriguing, but what do they do? Possible rationales for this cell behavior include: (1) forming precise binary connections with the possibility of forming mo...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/s0092-8674(03)00156-9
更新日期:2003-03-07 00:00:00
abstract::The archetypal human tumor suppressor p53 is considered to have unique transactivation properties. The assumption is based on the fact that additionally identified human p53 isoforms lack transcriptional activity. However, we provide evidence for the existence of an alternatively spliced p53 isoform (Deltap53) that ex...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2005.04.032
更新日期:2005-07-15 00:00:00
abstract::Morphogen gradients control body pattern by differentially regulating cellular behavior. Here, we analyze the molecular events underlying the primary response to the Dpp/BMP morphogen in Drosophila. Throughout development, Dpp transduction causes the graded transcriptional downregulation of the brinker (brk) gene. We ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00241-1
更新日期:2003-04-18 00:00:00
abstract::During embryogenesis, cells from the ventral and dorsal parts of the somites give rise to sclerotome and dermomyotome, respectively. Dermomyotome contains skeletal muscle precursors that are determined by the MyoD family of myogenic factors. We have isolated a novel myogenic repressor, I-mf (Inhibitor of MyoD family),...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80148-8
更新日期:1996-09-06 00:00:00
abstract::The non-receptor protein tyrosine phosphatase (PTP) SHP2, encoded by PTPN11, plays an essential role in RAS-mitogen-activated protein kinase (MAPK) signaling during normal development. It has been perplexing as to why both enzymatically activating and inactivating mutations in PTPN11 result in human developmental diso...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.09.002
更新日期:2020-10-15 00:00:00
abstract::We have isolated a dominant mutation in a gene called Son of sevenless (Sos) that is an allele-specific suppressor of the sevenless phenotype. This suppressor function is autonomously required in R7 and is sensitive to the dosage of the Sos and bride of sevenless genes. Loss-of-function alleles of Sos are recessive le...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90207-f
更新日期:1991-01-11 00:00:00
abstract::Little is known about how neutrophils and other cells establish a single zone of actin assembly during migration. A widespread assumption is that the leading edge prevents formation of additional fronts by generating long-range diffusible inhibitors or by sequestering essential polarity components. We use morphologica...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.10.050
更新日期:2012-01-20 00:00:00
abstract::Immunofluorescent staining of a pericellular matrix produced by cultured human embryonic skin fibroblasts showed a codistribution among fibronectin, heparan sulfate proteoglycans and part of the chondroitin sulfate in a fibrillar network. Isolated matrix in an "intact" form could be scraped off the dish after detergen...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90221-5
更新日期:1982-03-01 00:00:00
abstract::Immune responses involve coordination across cell types and tissues. However, studies in cancer immunotherapy have focused heavily on local immune responses in the tumor microenvironment. To investigate immune activity more broadly, we performed an organism-wide study in genetically engineered cancer models using mass...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.12.022
更新日期:2017-01-26 00:00:00
abstract::Genetic screening identifies the atypical tetraspanin TM4SF1 as a strong mediator of metastatic reactivation of breast cancer. Intriguingly, TM4SF1 couples the collagen receptor tyrosine kinase DDR1 to the cortical adaptor syntenin 2 and, hence, to PKCα. The latter kinase phosphorylates and activates JAK2, leading to ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.06.009
更新日期:2016-06-30 00:00:00
abstract::The lack of in vitro prostate cancer models that recapitulate the diversity of human prostate cancer has hampered progress in understanding disease pathogenesis and therapy response. Using a 3D organoid system, we report success in long-term culture of prostate cancer from biopsy specimens and circulating tumor cells....
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.08.016
更新日期:2014-09-25 00:00:00
abstract::Induction of senescence permanently restricts cellular proliferation after oncogenic stimulation thereby acting as a potent barrier to tumor development. The relevant effector proteins may therefore be fundamental to cancer development. A recent study identified IGFBP7 as a secreted factor mediating melanocyte senesce...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2010.04.021
更新日期:2010-05-14 00:00:00
abstract::We show that transfer of human fibroblast chromosome 11 (containing the human MyoD gene) from primary cells into 10T1/2 mouse fibroblasts by microcell fusion activates expression of the transferred human MyoD gene and converts these cells to myoblasts. Transfer of human chromosome 11 into B78 melanoma cells also leads...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90285-m
更新日期:1990-10-05 00:00:00