Abstract：
:We show that transfer of human fibroblast chromosome 11 (containing the human MyoD gene) from primary cells into 10T1/2 mouse fibroblasts by microcell fusion activates expression of the transferred human MyoD gene and converts these cells to myoblasts. Transfer of human chromosome 11 into B78 melanoma cells also leads to the activation of human MyoD. In contrast to the results where a single chromosome 11 is transferred, whole-cell hybrids between 10T1/2 cells and human skin fibroblasts do not express the myogenic phenotype; however, when specific human chromosomes are lost, myogenesis occurs. These results suggest that the MyoD locus is potentially functional in primary human fibroblasts, but is normally repressed in trans by a locus on a different human fibroblast chromosome.
journal_name
Celljournal_title
Cellauthors
Thayer MJ,Weintraub Hdoi
10.1016/0092-8674(90)90285-msubject
Has Abstractpub_date
1990-10-05 00:00:00pages
23-32issue
1eissn
0092-8674issn
1097-4172pii
0092-8674(90)90285-Mjournal_volume
63pub_type
杂志文章相关文献
CELL文献大全abstract：:Twitter needs no introduction. This "microblogging" service has gained tremendous popularity in the 2 years since its launch. Yet, most scientists are steering clear of it. Laura Bonetta speaks to some who have found value in tweeting. ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2009.10.017
更新日期：2009-10-30 00:00:00
abstract：:Eukaryotic Argonaute proteins strictly mediate RNA-guided RNA interference. In contrast, prokaryotic Argonautes can utilize DNA guides to target complementary DNA sequences to protect their hosts against invading DNA. In this issue of Cell, Jolly and colleagues demonstrate that Thermus thermophilus Argonaute additiona...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.08.037
更新日期：2020-09-17 00:00:00
abstract：:Intestinal peristalsis is a dynamic physiologic process influenced by dietary and microbial changes. It is tightly regulated by complex cellular interactions; however, our understanding of these controls is incomplete. A distinct population of macrophages is distributed in the intestinal muscularis externa. We demonst...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.04.050
更新日期：2014-07-17 00:00:00
abstract：:We analyzed a family of proteins from hepatoma cell nuclei that bind to interleukin-6 responsive elements (IL-6REs) of several acute-phase genes. This family is characterized by leucine zipper domains compatible with that of the CCAAT/enhancer binding protein (C/EBP). A cDNA clone coding for a member of the family, IL...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90459-r
更新日期：1990-11-02 00:00:00
abstract：:Based on the above mechanisms of mRNA degradation, an integrated model of mRNA turnover can be proposed (Figure 1). In this model, all polyadenylated mRNAs would be degraded by the deadenylation-dependent pathway at some rate. In addition to this default pathway, another layer of complexity would come from degradation...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/0092-8674(95)90326-7
更新日期：1995-04-21 00:00:00
abstract：:Fractionation of a microsomal detergent extract with ammonium sulfate allows separation of the signal recognition particle receptor (SR alpha), which is required for targeting of the nascent chain, from other microsomal proteins, such as signal peptidase, whose activity is displayed during subsequent translocation. Th...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90091-c
更新日期：1991-05-17 00:00:00
abstract：:We have isolated the genes encoding the largest subunit of all three classes of RNA polymerase from Trypanosoma brucei. While the pol II largest subunit is encoded by a single gene in all organisms examined to date, trypanosomes contain two copies of the gene. Both genes are expressed in the procyclic and bloodstream ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(89)90686-7
更新日期：1989-03-10 00:00:00
abstract：:The double-stranded RNA-activated protein kinase (PKR) was originally identified as a sensor of virus infection, but its function in the brain remains unknown. Here, we report that the lack of PKR enhances learning and memory in several behavioral tasks while increasing network excitability. In addition, loss of PKR i...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2011.11.029
更新日期：2011-12-09 00:00:00
abstract：:How transcription is silenced in early embryos has long been a mystery. In this issue, Guven-Ozkan et al. (2008) report that transcriptional repression during worm embryogenesis is mediated through sequestration of the general transcription factor TAF-4 and is regulated by mechanisms that orchestrate the transition be...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2008.09.021
更新日期：2008-10-03 00:00:00
abstract：:Interactions between similar but not identical (homeologous) DNA sequences play an important biological role in the evolution of genes and genomes. To gain insight into the underlying molecular mechanism(s) of genetic recombination, we have studied inter- and intramolecular homeologous recombination in S. cerevisiae d...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90434-e
更新日期：1992-08-21 00:00:00
abstract：:Lipid composition affects the biophysical properties of membranes that provide a platform for receptor-mediated cellular signaling. To study the regulatory role of membrane lipid composition, we combined genetic perturbations of sphingolipid metabolism with the quantification of diverse steps in Toll-like receptor (TL...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.05.051
更新日期：2015-07-02 00:00:00
abstract：:The Wee1 kinase phosphorylates and inhibits cyclin-dependent kinase 1 (Cdk1), thereby delaying entry into mitosis until appropriate conditions have been met. An understanding of the mechanisms that regulate Wee1 should provide new insight into how cells make the decision to enter mitosis. We report here that Swe1, the...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2005.05.029
更新日期：2005-08-12 00:00:00
abstract：:The existence of pseudogenes and the observation of intron loss suggest that RNA can serve as an intermediate in recombination. We used a HIS3 reporter gene to show that RNA-mediated recombination occurs in yeast. His3+ prototroph formation required transcription and expression of the retrotransposon Ty. Two RNA-media...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90187-4
更新日期：1991-10-18 00:00:00
abstract：:Nociceptin and its receptor are widely distributed throughout the brain in regions associated with reward behavior, yet how and when they act is unknown. Here, we dissected the role of a nociceptin peptide circuit in reward seeking. We generated a prepronociceptin (Pnoc)-Cre mouse line that revealed a unique subpopula...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.06.034
更新日期：2019-07-25 00:00:00
abstract：:Prions, self-propagating protein structures that can be transmitted between cells and different organisms, usually consist of ordered protein aggregates. Alberti et al. (2009) now present a systematic approach for the discovery of new prions that expands the spectrum of their biological functions. ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2009.03.030
更新日期：2009-04-03 00:00:00
abstract：:The gut epithelium self-renews every several days, providing an important innate defense system that limits bacterial colonization. Nevertheless, many bacterial pathogens, including Shigella, efficiently colonize the intestinal epithelium. Here, we show that the Shigella effector IpaB, when delivered into epithelial c...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2007.06.043
更新日期：2007-08-24 00:00:00
abstract：:ROAM mutations cause overproduction in S. cerevisiae. Overproduction of ROAM mutant gene products is less in MATa/MAT alpha diploid strains which cannot conjugate than in haplolid strains which can. Overproduction occurs in diploid strains capable of mating whether or not they are capable of sporulating. Overproductio...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(80)90353-0
更新日期：1980-11-01 00:00:00
abstract：:Assessing the real-world impact of biomedical research is notoriously difficult. Here, we present the framework for building a prospective science-centered information system from scratch that has been afforded by the Sidra Medical and Research Center in Qatar. This experiment is part of the global conversation on max...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.03.004
更新日期：2016-03-24 00:00:00
abstract：:A recent paper in Cell (Yao et al., 2006) and two papers in Developmental Cell (Tenzen et al., 2006; Zhang et al., 2006) identify a new receptor component for Hedgehog, a key morphogen in embryonic development. Many other proteins that bind to Hedgehog in the extracellular matrix or on the cell surface have been ident...
journal_title：Cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.cell.2006.04.016
更新日期：2006-05-05 00:00:00
abstract：:Observational learning is a powerful survival tool allowing individuals to learn about threat-predictive stimuli without directly experiencing the pairing of the predictive cue and punishment. This ability has been linked to the anterior cingulate cortex (ACC) and the basolateral amygdala (BLA). To investigate how inf...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.04.004
更新日期：2018-05-31 00:00:00
abstract：:Certain mutations can cause proteins to accumulate in neurons, leading to neurodegeneration. We recently showed, however, that upregulation of a wild-type protein, Ataxin1, caused by haploinsufficiency of its repressor, the RNA-binding protein Pumilio1 (PUM1), also causes neurodegeneration in mice. We therefore search...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.02.006
更新日期：2018-02-22 00:00:00
abstract：:A "switch" from oxidative phosphorylation (OXPHOS) to aerobic glycolysis is a hallmark of T cell activation and is thought to be required to meet the metabolic demands of proliferation. However, why proliferating cells adopt this less efficient metabolism, especially in an oxygen-replete environment, remains incomplet...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.05.016
更新日期：2013-06-06 00:00:00
abstract：:On each side of the midline of the Drosophila CNS, axons are organized into a series of parallel pathways. Here we show that the midline repellent Slit, previously identified as a short-range signal that regulates midline crossing, also functions at long range to pattern these longitudinal pathways. In this long-range...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)00207-5
更新日期：2000-12-22 00:00:00
abstract：:For the faithful initiation of transcription of exogenously added fibroin gene, a crude cellular extract was prepared from the posterior silk glands where fibroin gene is specifically transcribed. The faithful initiation was demonstrated by the assay with use of truncated fibroin gene templates and confirmed by nuclea...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90371-8
更新日期：1981-11-01 00:00:00
abstract：:Proteolytic fragments of human plasma fibronectin were used to identify monoclonal antibodies reacting with the various domains of fibronectin. One of these antibodies, which reacts with cell-attachment-promoting fragments of fibronectin, inhibits attachment of cells to fibronectin-coated surfaces. A cell-attachment-p...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90308-1
更新日期：1981-10-01 00:00:00
abstract：:E. coli sigma 54 protein confers on promoters containing its recognition sequence the ability to be activated from distant DNA sites. Its functional domains include two leucine zipper motifs, an acidic region, and a glutamine-rich domain. Several domains were disrupted and the assembly of mutant transcription complexe...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90269-k
更新日期：1990-09-07 00:00:00
abstract：:The coactivator PGC-1alpha is a key regulator of mitochondrial biogenesis and respiration, mediating expression of several transcription factors required for these programs. Three new studies reveal that PGC-1alpha expression is downregulated in patients with Huntington's disease (HD) and in several animal models of t...
journal_title：Cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.cell.2006.10.023
更新日期：2006-11-03 00:00:00
abstract：:Most human epithelial tumors harbor numerous alterations, making it difficult to predict which genes are required for tumor survival. To systematically identify cancer dependencies, we analyzed 501 genome-scale loss-of-function screens performed in diverse human cancer cell lines. We developed DEMETER, an analytical f...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.06.010
更新日期：2017-07-27 00:00:00
abstract：:The HIV auxiliary protein Vpr potently blocks the cell cycle at the G2/M transition. Here, we show that G2/M arrest results from untimely activation of the structure-specific endonuclease (SSE) regulator SLX4 complex (SLX4com) by Vpr, a process that requires VPRBP-DDB1-CUL4 E3-ligase complex. Direct interaction of Vpr...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.12.011
更新日期：2014-01-16 00:00:00
abstract：:Cytotoxic brain edema triggered by neuronal swelling is the chief cause of mortality following brain trauma and cerebral infarct. Using fluorescence lifetime imaging to analyze contributions of intracellular ionic changes in brain slices, we find that intense Na(+) entry triggers a secondary increase in intracellular ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.03.029
更新日期：2015-04-23 00:00:00
