Abstract:
:5, 6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB; 75 muM) inhibited only 60-75% of nuclear heterogeneous RNA (hnRNA) synthesis in HeLa cells, while the appearance of labeled cytoplasmic poly(A)-containing messenger RNA (mRNA) was reduced by approximately 95%. DRB-sensitive and resistant transcripts spanned the entire size range of hnRNA molecules. The relative poly(A) content of DRB-resistant hnRNA molecules was the same as that of normal hnRNA. In contrast to the effects of 3'deoxyadenosine, DRB appeared to inhibit the initiation of hnRNA chains, but did not directly interfere with labeling of poly(A). We present an interpretation of these results and propose how the results might be used to gain more information about nuclear RNA metabolism.
journal_name
Celljournal_title
Cellauthors
Sehgal PB,Darnell JE Jr,Tamm Idoi
10.1016/0092-8674(76)90092-1subject
Has Abstractpub_date
1976-11-01 00:00:00pages
473-80issue
3eissn
0092-8674issn
1097-4172pii
0092-8674(76)90092-1journal_volume
9pub_type
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