Abstract:
:Transposon Tn10 inserts at many sites in the bacterial chromosome, but preferentially inserts at particular hotspots. We believe we have identified the target DNA signal responsible for this specificity. We have determined the DNA sequences of 11 Tn10 insertion sites and identified a particular 6 base pair (bp) symmetrical consensus sequence (GCTNAGC) common to those sites. The sequences at some sites differ from the consensus sequence but only in limited and well defined ways. The sequences at some sites differ from the consensus sequence than do sequences at other sites, and the consensus sequence and closely related sequences are generally absent from potential target regions where Tn10 is known not to insert. Other aspects of the target DNA can significantly influence the efficiency with which a particular target site sequence is used. The 6 bp consensus sequence is symmetrically located within the 9 bp target DNA sequence that is cleaved and duplicated during Tn10 insertion. This juxtaposition of recognition and cleavage sites plus the symmetry of the perfect consensus sequence suggest that the target DNA may be both recognized and cleaved by the symmetrically disposed subunits of a single protein, as suggested for type II restriction endonucleases. There is plausible homology between the consensus sequence and the very ends of Tn10, compatible with recognition of transposon ends and target DNA by the same protein. The sequences of actual insertion sites deviate from the perfect consensus sequence in a way which suggests that the 6 bp specificity determinant may be recognized through protein-DNA contacts along the major groove of the DNA double helix.
journal_name
Celljournal_title
Cellauthors
Halling SM,Kleckner Ndoi
10.1016/0092-8674(82)90385-3subject
Has Abstractpub_date
1982-01-01 00:00:00pages
155-63issue
1eissn
0092-8674issn
1097-4172pii
0092-8674(82)90385-3journal_volume
28pub_type
杂志文章相关文献
CELL文献大全abstract::Clec16a has been identified as a disease susceptibility gene for type 1 diabetes, multiple sclerosis, and adrenal dysfunction, but its function is unknown. Here we report that Clec16a is a membrane-associated endosomal protein that interacts with E3 ubiquitin ligase Nrdp1. Loss of Clec16a leads to an increase in the N...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.05.016
更新日期:2014-06-19 00:00:00
abstract::Heat shock and other proteotoxic stresses cause accumulation of nonnative proteins that trigger activation of heat shock protein (Hsp) genes. A chaperone/Hsp functioning as repressor of heat shock transcription factor (HSF) could make activation of hsp genes dependent on protein unfolding. In a novel in vitro system, ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81588-3
更新日期:1998-08-21 00:00:00
abstract::Phosphoinositide 3 kinase (PI3K) is a key signaling enzyme implicated in receptor-stimulated mitogenesis, oxidative bursting in neutrophils, membrane ruffling, and glucose uptake. A PI3K has already been purified, cloned, and shown to be regulated by receptors that act via tyrosine kinase-dependent regulatory mechanis...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90237-2
更新日期:1994-04-08 00:00:00
abstract::The mammalian circadian timing system is composed of a central pacemaker in the suprachiasmatic nucleus (SCN) of the brain and subsidiary oscillators in most peripheral cell types. While oscillators in SCN neurons are known to function in a self-sustained fashion, peripheral oscillators have been thought to damp rapid...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2004.11.015
更新日期:2004-11-24 00:00:00
abstract::Calmodulin is implicated as the primary transducer of the calcium signal in pancreatic beta cells, where it is present at very high concentrations. We have produced three lines of transgenic mice carrying a calmodulin minigene regulated by the rat insulin II promoter. Immunohistochemistry and hybridization analyses in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90505-9
更新日期:1989-09-22 00:00:00
abstract::Yeast mutants lacking meiotic proteins Zip1, Zip2, Zip3, Mer3, and/or Msh5 (ZMMs) were analyzed for recombination, synaptonemal complex (SC), and meiotic progression. At 33 degrees C, recombination-initiating double-strand breaks (DSBs) and noncrossover products (NCRs) form normally while formation of single-end invas...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(04)00292-2
更新日期:2004-04-02 00:00:00
abstract::Radioactive alpha factor is degraded to discrete biologically inactive fragments by the target a cells of S. cerevisiae, but not by alpha cells which make the pheromone. The pattern of cleavage products and sequence analysis of one fragment indicated that the first scission occurred between leucine 6 and lysine 7. The...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90117-x
更新日期:1979-11-01 00:00:00
abstract::The mitochondrial unfolded protein response (UPRmt) can be triggered in a cell-non-autonomous fashion across multiple tissues in response to mitochondrial dysfunction. The ability to communicate information about the presence of mitochondrial stress enables a global response that can ultimately better protect an organ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.06.029
更新日期:2018-08-09 00:00:00
abstract::Viruses are adept at evolving or co-opting genomic elements that allow them to maximize their replication potential in the infected host. This evolutionary plasticity makes viruses an invaluable system to identify new mechanisms used not only by viruses but also by vertebrate cells to modulate gene expression. Here, I...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.01.048
更新日期:2009-02-20 00:00:00
abstract::The HIV-1 Tat protein is a potent trans-activator essential for virus replication. We reported previously that HIV-1 Tat peptides containing residues 37-48 (mainly region II), a possible activating region, and residues 49-57 (region III), a nuclear targeting and putative nucleic acid binding region, possess minimal bu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90417-0
更新日期:1989-07-14 00:00:00
abstract::Genetic screening identifies the atypical tetraspanin TM4SF1 as a strong mediator of metastatic reactivation of breast cancer. Intriguingly, TM4SF1 couples the collagen receptor tyrosine kinase DDR1 to the cortical adaptor syntenin 2 and, hence, to PKCα. The latter kinase phosphorylates and activates JAK2, leading to ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.06.009
更新日期:2016-06-30 00:00:00
abstract::Dysfunctional mitochondria accumulate in many human diseases. Accordingly, mitophagy, which removes these mitochondria through lysosomal degradation, is attracting broad attention. Due to uncertainties in the operational principles of conventional mitophagy probes, however, the specificity and quantitativeness of thei...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.04.025
更新日期:2020-05-28 00:00:00
abstract::Cancer cell of origin is difficult to identify by analyzing cells within terminal stage tumors, whose identity could be concealed by the acquired plasticity. Thus, an ideal approach to identify the cell of origin is to analyze proliferative abnormalities in distinct lineages prior to malignancy. Here, we use mosaic an...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.06.014
更新日期:2011-07-22 00:00:00
abstract::Metabolic coordination between neurons and astrocytes is critical for the health of the brain. However, neuron-astrocyte coupling of lipid metabolism, particularly in response to neural activity, remains largely uncharacterized. Here, we demonstrate that toxic fatty acids (FAs) produced in hyperactive neurons are tran...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.04.001
更新日期:2019-05-30 00:00:00
abstract::While the nuclear lamin proteins (A, B, and C) assemble specifically at the surface of the nuclear membrane, their sequences do not reveal stretches of hydrophobic amino acids that might explain their association with the nuclear membranes. However, the A and B lamin proteins possess Ras-like C-terminal CaaX sequence ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90753-8
更新日期:1989-12-22 00:00:00
abstract::The immortal strand hypothesis posits that the propensity of stem cell compartments to give rise to cancer in later life can be minimized if stem cells, during the process of self-renewal, retain those DNA strands with the fewest mutations acquired during DNA replication. In this Essay, I explore evidence in support o...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2007.06.019
更新日期:2007-06-29 00:00:00
abstract::Transcriptional analysis of SV40 late promoter mutants indicates that the DNA sequence 5'-GGTACCTAACC-3' (map positions 294-304) is important in the control of SV40 late RNA expression. C to T base substitutions at map positions 298, 299 and 304 increased initiation of late RNA synthesis at map position 325 five to te...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90318-x
更新日期:1982-12-01 00:00:00
abstract::The rate of incorporation of leucine into protein, the rate of polypeptide elongation and termination, and the relative quantity and size of polysomes were analyzed in mouse L cells grown in suspension culture at various temperatures between 0 degrees C and 36 degrees C. Between 10 degrees C and 36 degrees C protein s...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(75)90153-1
更新日期:1975-04-01 00:00:00
abstract::The distribution of sense and antisense strand DNA mutations on transcribed duplex DNA contributes to the development of immune and neural systems along with the progression of cancer. Because developmentally matured B cells undergo biologically programmed strand-specific DNA mutagenesis at focal DNA/RNA hybrid struct...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.03.043
更新日期:2017-04-20 00:00:00
abstract::The interplay of genetics and the environment in the etiology of Alzheimer's disease (AD) is not well understood. Now, Lazarov and coworkers show that a simple paradigm of environmental enrichment alleviates amyloid burden and alters disease-associated gene expression changes in a double transgenic mouse model of AD. ...
journal_title:Cell
pub_type: 评论,杂志文章,评审
doi:10.1016/j.cell.2005.02.027
更新日期:2005-03-11 00:00:00
abstract::gamma-Irradiation of human diploid fibroblasts in the G1 interval caused arrest of the cell cycle prior to S phase. This cell cycle block was correlated with a lack of activation of both cyclin E-Cyclin-dependent kinase 2 (Cdk2) and cyclin A-Cdk2 kinases and depended on wild-type p53. Although the accumulation of cycl...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90379-4
更新日期:1994-03-25 00:00:00
abstract::Targeting of the RAS pathway has long been a critical therapeutic challenge in oncology. Burgess et al. examine how the relative expression of mutant and wild-type KRAS modulates clonal fitness and sensitivity to MEK inhibitors in a model of KrasG12D mutant acute myeloid leukemia and propose its use as a predictive bi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.02.013
更新日期:2017-02-23 00:00:00
abstract::Plant seedling development is capable of following 1 of 2 distinct morphogenic pathways: skotomorphogenesis in darkness and photomorphogenesis in light. Dark-grown Arabidopsis seedlings with recessive mutations at the constitutively photomorphogenic (COP1) locus indicate that the wild-type COP1 protein represses photo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90555-q
更新日期:1992-11-27 00:00:00
abstract::The proportion of BFU-E normally engaged in DNA synthesis is low in adult B6 (C57BL/6) mice of genotype Fv2rr (resistant to Friend erythroleukemia virus), as shown by 3H-thymidine or hydroxyurea "cell suicide" experiments in vivo and vitro. When bone marrow cells from these mice were subjected to a single wash in alph...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90306-8
更新日期:1981-10-01 00:00:00
abstract::The scrapie (PrPSc) and cellular (PrPC) prion proteins are encoded by the same gene, and their different properties are thought to arise from posttranslational modifications. We have found a phosphatidylinositol glycolipid on both PrPC and PrP 27-30 (derived from PrPSc by limited proteolysis at the amino terminus). Et...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90150-4
更新日期:1987-10-23 00:00:00
abstract::Cells released from primary tumors seed metastases to specific organs by a nonrandom process, implying the involvement of biologically selective mechanisms. Based on clinical, functional, and molecular evidence, we show that the cytokine TGFbeta in the breast tumor microenvironment primes cancer cells for metastasis t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2008.01.046
更新日期:2008-04-04 00:00:00
abstract::Receptor dimerization is ubiquitous to the action of all receptor tyrosine kinases, and in the case of dimeric ligands, such as the stem cell factor (SCF), it was attributed to ligand bivalency. However, by using a dimerization-inhibitory monoclonal antibody to the SCF receptor, we confined a putative dimerization sit...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90455-7
更新日期:1995-01-13 00:00:00
abstract::Adenovirus E1B 19 kDa protein protects against cell death induced by viral infection and certain external stimuli. The Bcl-2 protein can functionally substitute for the E1B 19 kDa protein. To identify cellular targets for the 19 kDa protein, we used the two-hybrid screen in yeast. We have isolated cDNAs for three diff...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90202-x
更新日期:1994-10-21 00:00:00
abstract::Complex multicellular organisms, such as mammals, express two complete sets of chromosomes per nucleus, combining the genetic material of both parents. However, epigenetic studies have demonstrated violations to this rule that are necessary for mammalian physiology; the most notable parental allele expression phenomen...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2019.01.043
更新日期:2019-02-21 00:00:00
abstract::Two decades of research identified more than a dozen clock genes and defined a biochemical feedback mechanism of circadian oscillator function. To identify additional clock genes and modifiers, we conducted a genome-wide small interfering RNA screen in a human cellular clock model. Knockdown of nearly 1000 genes reduc...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.08.031
更新日期:2009-10-02 00:00:00