Abstract：
:As axons grow past intermediate targets, they change their responsiveness to guidance cues. Local upregulation of receptor expression is involved, but the mechanisms for this are not clear. Here protein synthesis is traced within individual axons by introducing RNAs encoding visualizable reporters. Individual severed axons and growth cones can translate proteins and also export them to the cell surface. As axons reach the spinal cord midline, EphA2 is among the receptors upregulated on at least some distal axon segments. Midline reporter upregulation is recapitulated by part of the EphA2 mRNA 3' untranslated region, which is highly conserved and includes known translational control sequences. These results show axons contain all the machinery for protein translation and cell surface expression, and they reveal a potentially general and flexible RNA-based mechanism for regulation localized within a subregion of the axon.
journal_name
Celljournal_title
Cellauthors
Brittis PA,Lu Q,Flanagan JGdoi
10.1016/s0092-8674(02)00813-9subject
Has Abstractpub_date
2002-07-26 00:00:00pages
223-35issue
2eissn
0092-8674issn
1097-4172pii
S0092867402008139journal_volume
110pub_type
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