Abstract:
:Long-term severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) shedding was observed from the upper respiratory tract of a female immunocompromised individual with chronic lymphocytic leukemia and acquired hypogammaglobulinemia. Shedding of infectious SARS-CoV-2 was observed up to 70 days, and of genomic and subgenomic RNA up to 105 days, after initial diagnosis. The infection was not cleared after the first treatment with convalescent plasma, suggesting a limited effect on SARS-CoV-2 in the upper respiratory tract of this individual. Several weeks after a second convalescent plasma transfusion, SARS-CoV-2 RNA was no longer detected. We observed marked within-host genomic evolution of SARS-CoV-2 with continuous turnover of dominant viral variants. However, replication kinetics in Vero E6 cells and primary human alveolar epithelial tissues were not affected. Our data indicate that certain immunocompromised individuals may shed infectious virus longer than previously recognized. Detection of subgenomic RNA is recommended in persistently SARS-CoV-2-positive individuals as a proxy for shedding of infectious virus.
journal_name
Celljournal_title
Cellauthors
Avanzato VA,Matson MJ,Seifert SN,Pryce R,Williamson BN,Anzick SL,Barbian K,Judson SD,Fischer ER,Martens C,Bowden TA,de Wit E,Riedo FX,Munster VJdoi
10.1016/j.cell.2020.10.049subject
Has Abstractpub_date
2020-12-23 00:00:00pages
1901-1912.e9issue
7eissn
0092-8674issn
1097-4172pii
S0092-8674(20)31456-2journal_volume
183pub_type
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