Case Study: Prolonged Infectious SARS-CoV-2 Shedding from an Asymptomatic Immunocompromised Individual with Cancer.


:Long-term severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) shedding was observed from the upper respiratory tract of a female immunocompromised individual with chronic lymphocytic leukemia and acquired hypogammaglobulinemia. Shedding of infectious SARS-CoV-2 was observed up to 70 days, and of genomic and subgenomic RNA up to 105 days, after initial diagnosis. The infection was not cleared after the first treatment with convalescent plasma, suggesting a limited effect on SARS-CoV-2 in the upper respiratory tract of this individual. Several weeks after a second convalescent plasma transfusion, SARS-CoV-2 RNA was no longer detected. We observed marked within-host genomic evolution of SARS-CoV-2 with continuous turnover of dominant viral variants. However, replication kinetics in Vero E6 cells and primary human alveolar epithelial tissues were not affected. Our data indicate that certain immunocompromised individuals may shed infectious virus longer than previously recognized. Detection of subgenomic RNA is recommended in persistently SARS-CoV-2-positive individuals as a proxy for shedding of infectious virus.






Avanzato VA,Matson MJ,Seifert SN,Pryce R,Williamson BN,Anzick SL,Barbian K,Judson SD,Fischer ER,Martens C,Bowden TA,de Wit E,Riedo FX,Munster VJ




Has Abstract


2020-12-23 00:00:00
















  • Identification of a multicomponent complex required for outer membrane biogenesis in Escherichia coli.

    abstract::Gram-negative bacteria have an outer membrane (OM) that functions as a barrier to protect the cell from toxic compounds such as antibiotics and detergents. The OM is a highly asymmetric bilayer composed of phospholipids, glycolipids, and proteins. Assembly of this essential organelle occurs outside the cytoplasm in an...


    pub_type: 杂志文章


    authors: Wu T,Malinverni J,Ruiz N,Kim S,Silhavy TJ,Kahne D

    更新日期:2005-04-22 00:00:00

  • DNA structural variations in the E. coli tyrT promoter.

    abstract::X-ray studies have established that the structure of a right-handed, Watson-Crick double helix can change from place to place along its length as a function of base sequence. The base pairs transmit deformations out to the phosphate backbone, where they can then be recognized by proteins and other DNA-binding reagents...


    pub_type: 杂志文章


    authors: Drew HR,Travers AA

    更新日期:1984-06-01 00:00:00

  • Architecture of a coat for the nuclear pore membrane.

    abstract::The symmetric core of the nuclear pore complex can be considered schematically as a series of concentric cylinders. A peripheral cylinder coating the pore membrane contains the previously characterized, elongated heptamer that harbors Sec13-Nup145C in its middle section. Strikingly, Sec13-Nup145C crystallizes as a het...


    pub_type: 杂志文章


    authors: Hsia KC,Stavropoulos P,Blobel G,Hoelz A

    更新日期:2007-12-28 00:00:00

  • Separation of drug transport and chloride channel functions of the human multidrug resistance P-glycoprotein.

    abstract::The human multidrug resistance P-glycoprotein is an active transporter that pumps cytotoxic drugs out of cells. Expression of P-glycoprotein is also associated with a volume-activated chloride channel. Here we address the relationship between these two functions. Drug transport requires ATP hydrolysis while, in contra...


    pub_type: 杂志文章


    authors: Gill DR,Hyde SC,Higgins CF,Valverde MA,Mintenig GM,Sepúlveda FV

    更新日期:1992-10-02 00:00:00

  • A role for hematopoietic stem cells in promoting angiogenesis.

    abstract::Angiogenesis is an important event for embryonic organogenesis as well as for tissue repair in the adult. Here, we show that hematopoietic stem cells (HSCs) play important roles for angiogenesis during embryogenesis. To investigate the role of HSCs in endothelial cell (EC) development, we analyzed AML1-deficient embry...


    pub_type: 杂志文章


    authors: Takakura N,Watanabe T,Suenobu S,Yamada Y,Noda T,Ito Y,Satake M,Suda T

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  • Allele-Specific HLA Loss and Immune Escape in Lung Cancer Evolution.

    abstract::Immune evasion is a hallmark of cancer. Losing the ability to present neoantigens through human leukocyte antigen (HLA) loss may facilitate immune evasion. However, the polymorphic nature of the locus has precluded accurate HLA copy-number analysis. Here, we present loss of heterozygosity in human leukocyte antigen (L...


    pub_type: 杂志文章


    authors: McGranahan N,Rosenthal R,Hiley CT,Rowan AJ,Watkins TBK,Wilson GA,Birkbak NJ,Veeriah S,Van Loo P,Herrero J,Swanton C,TRACERx Consortium.

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  • ATP-dependent fusion of liposomes with the Golgi apparatus of perforated cells.

    abstract::We examined the interactions of lipid vesicles (liposomes) labeled with various fluorescent markers with the intracellular membranes of semi-intact ("perforated") fibroblasts. When incubations were performed in the presence of an ATP-regenerating system, both vesicle lipids and entrapped water soluble markers were tra...


    pub_type: 杂志文章


    authors: Kobayashi T,Pagano RE

    更新日期:1988-12-02 00:00:00

  • Growth-rate-dependent regulation of ribosome synthesis in E. coli: expression of the lacZ and galK genes fused to ribosomal promoters.

    abstract::Hybrid transducing phages were constructed in vitro that carry the galK gene fused to each of three ribosomal promoters: the promotor for an rRNA operon (rrnE); the promoter for the spec r protein operon and the promotor for the alpha r protein operon. We also constructed hybrid transducing phages that carry the IacZ ...


    pub_type: 杂志文章


    authors: Miura A,Krueger JH,Itoh S,de Boer HA,Nomura M

    更新日期:1981-09-01 00:00:00

  • Integration of Parallel Opposing Memories Underlies Memory Extinction.

    abstract::Accurately predicting an outcome requires that animals learn supporting and conflicting evidence from sequential experience. In mammals and invertebrates, learned fear responses can be suppressed by experiencing predictive cues without punishment, a process called memory extinction. Here, we show that extinction of av...


    pub_type: 杂志文章


    authors: Felsenberg J,Jacob PF,Walker T,Barnstedt O,Edmondson-Stait AJ,Pleijzier MW,Otto N,Schlegel P,Sharifi N,Perisse E,Smith CS,Lauritzen JS,Costa M,Jefferis GSXE,Bock DD,Waddell S

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  • Reiteration frequency of the gene coding for the small subunit of ribulose--1,5--bisphosphate carboxylase.

    abstract::The messenger RNA coding for the precursor of the small subunit of ribulose--1,5--bisphosphate (Ru-P2) carboxylase has been partially purified from pea leaves. The mRNA has a size of 11.5S, is approximately 800 nucleotides long and, on cell-free translation, directs the synthesis of a single major polypeptide of 20,00...


    pub_type: 杂志文章


    authors: Cashmore AR

    更新日期:1979-06-01 00:00:00

  • Functional analysis of a growth factor-responsive transcription factor complex.

    abstract::Serum response factor (SRF) forms a ternary complex at the c-fos serum response element (SRE) with an accessory factor, Elk-1. We constructed altered-binding specificity derivatives of SRF and Elk-1 that form a ternary complex at a mutated, inactive SRE; like Elk-1, the Elk-1 variant only binds its target as part of a...


    pub_type: 杂志文章


    authors: Hill CS,Marais R,John S,Wynne J,Dalton S,Treisman R

    更新日期:1993-04-23 00:00:00

  • The genetic basis for specific anosmia to isovaleric acid in the mouse.

    abstract::The detection and discrimination of odorants in mammals is thought to be mediated by a family of 100-1000 seven transmembrane domain receptor proteins, although none of these putative olfactory receptors have been shown to bind individual odorants with high affinity. We have used a genetic approach to identify the gen...


    pub_type: 杂志文章


    authors: Griff IC,Reed RR

    更新日期:1995-11-03 00:00:00

  • Interspecies sex and taste.

    abstract::Different species of fruit flies share habitats but are believed to mate with each other only rarely. In this issue, Fan et al. show that interspecies mating is inhibited by the taste receptor Gr32a (Gustatory receptor 32a) and a neural circuit in which it functions. ...


    pub_type: 评论,杂志文章


    authors: Koh TW,Carlson JR

    更新日期:2013-07-03 00:00:00

  • Spinophilin facilitates dephosphorylation of doublecortin by PP1 to mediate microtubule bundling at the axonal wrist.

    abstract::The axonal shafts of neurons contain bundled microtubules, whereas extending growth cones contain unbundled microtubule filaments, suggesting that localized activation of microtubule-associated proteins (MAP) at the transition zone may bundle these filaments during axonal growth. Dephosphorylation is thought to lead t...


    pub_type: 杂志文章


    authors: Bielas SL,Serneo FF,Chechlacz M,Deerinck TJ,Perkins GA,Allen PB,Ellisman MH,Gleeson JG

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  • Wnt signaling: multivesicular bodies hold GSK3 captive.

    abstract::Two key events in Wnt signal transduction, receptor endocytosis and inactivation of Glycogen Synthase Kinase 3 (GSK3), remain incompletely understood. Taelman et al. (2010) discover that Wnt signaling inactivates GSK3 by sequestering the enzyme in multivesicular bodies, thus linking these two events and providing a ne...


    pub_type: 评论,杂志文章


    authors: Niehrs C,Acebron SP

    更新日期:2010-12-23 00:00:00

  • Nine introns with conserved boundary sequences in the Euglena gracilis chloroplast ribulose-1,5-bisphosphate carboxylase gene.

    abstract::The single, chloroplast encoded gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL) from Euglena gracilis is found to contain nine intervening sequences. The intervening sequences were identified by heteroduplex analysis between Euglena rbcL and the non-intron-containing rbcL from Spin...


    pub_type: 杂志文章


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  • A notch-independent activity of suppressor of hairless is required for normal mechanoreceptor physiology.

    abstract::Suppressor of Hairless [Su(H)]/Lag-1/RBP-Jkappa/CBF1 is the only known transducing transcription factor for Notch receptor signaling. Here, we show that Su(H) has three distinct functions in the development of external mechanosensory organs in Drosophila: Notch-dependent transcriptional activation and a novel auto-rep...


    pub_type: 杂志文章


    authors: Barolo S,Walker RG,Polyanovsky AD,Freschi G,Keil T,Posakony JW

    更新日期:2000-12-08 00:00:00

  • Yeast histone H4 N-terminal sequence is required for promoter activation in vivo.

    abstract::To search for histone domains that may regulate transcription in vivo, we made deletions and amino acid substitutions in the histone N-termini of S. cerevisiae. Histone H4 N-terminal residues 4-23, which include the extremely conserved, reversibly acetylated lysines (at positions 5, 8, 12, and 16), were found to encom...


    pub_type: 杂志文章


    authors: Durrin LK,Mann RK,Kayne PS,Grunstein M

    更新日期:1991-06-14 00:00:00

  • The biological and chemical basis for tissue-selective amyloid disease.

    abstract::Factors controlling the onset and progression of extracellular amyloid diseases remain largely unknown. Central to disease etiology is the efficiency of the endoplasmic reticulum (ER) machinery that targets destabilized mutant proteins for degradation and the enhanced tendency of these variants to aggregate if secrete...


    pub_type: 杂志文章


    authors: Sekijima Y,Wiseman RL,Matteson J,Hammarström P,Miller SR,Sawkar AR,Balch WE,Kelly JW

    更新日期:2005-04-08 00:00:00

  • Structure of the hemagglutinin precursor cleavage site, a determinant of influenza pathogenicity and the origin of the labile conformation.

    abstract::The membrane fusion potential of influenza HA, like many viral membrane-fusion glycoproteins, is generated by proteolytic cleavage of a biosynthetic precursor. The three-dimensional structure of ectodomain of the precursor HA0 has been determined and compared with that of cleaved HA. The cleavage site is a prominent s...


    pub_type: 杂志文章


    authors: Chen J,Lee KH,Steinhauer DA,Stevens DJ,Skehel JJ,Wiley DC

    更新日期:1998-10-30 00:00:00

  • Transcriptional activation of cloned human beta-globin genes by viral immediate-early gene products.

    abstract::When the human beta-globin gene is transfected into Hela cells, no beta-globin RNA is detected unless the gene is linked to a viral transcription enhancer. In this paper we show that trans-acting adenovirus and herpesvirus (pseudorabies) transcriptional regulatory proteins can circumvent this enhancer requirement for ...


    pub_type: 杂志文章


    authors: Green MR,Treisman R,Maniatis T

    更新日期:1983-11-01 00:00:00

  • The primary structure of the putative oncogene pim-1 shows extensive homology with protein kinases.

    abstract::We have shown previously that the putative oncogene pim-1 is frequently activated by provirus insertion in murine leukemia virus-induced T cell lymphomas. Here we describe the structure of the pim-1 gene as determined by sequencing genomic and cDNA clones. The gene has an open reading frame, encoding a protein of 313 ...


    pub_type: 杂志文章


    authors: Selten G,Cuypers HT,Boelens W,Robanus-Maandag E,Verbeek J,Domen J,van Beveren C,Berns A

    更新日期:1986-08-15 00:00:00

  • M-MuLV-induced leukemogenesis: integration and structure of recombinant proviruses in tumors.

    abstract::M-MuLV-specific DNA probes were used to establish the state of integration and amplification of recombinant proviral sequences in Moloney virus-induced tumors of Balb/Mo, Balb/c and 129 mice. The somatically acquired viral sequences contain both authentic M-MuLV genomes and recombinants of M-MuLV with endogenous viral...


    pub_type: 杂志文章


    authors: van der Putten H,Quint W,van Raaij J,Maandag ER,Verma IM,Berns A

    更新日期:1981-06-01 00:00:00

  • Nitric Oxide as a Switching Mechanism between Axon Degeneration and Regrowth during Developmental Remodeling.

    abstract::During development, neurons switch among growth states, such as initial axon outgrowth, axon pruning, and regrowth. By studying the stereotypic remodeling of the Drosophila mushroom body (MB), we found that the heme-binding nuclear receptor E75 is dispensable for initial axon outgrowth of MB γ neurons but is required ...


    pub_type: 杂志文章


    authors: Rabinovich D,Yaniv SP,Alyagor I,Schuldiner O

    更新日期:2016-01-14 00:00:00

  • Glioma stem cell proliferation and tumor growth are promoted by nitric oxide synthase-2.

    abstract::Malignant gliomas are aggressive brain tumors with limited therapeutic options, and improvements in treatment require a deeper molecular understanding of this disease. As in other cancers, recent studies have identified highly tumorigenic subpopulations within malignant gliomas, known generally as cancer stem cells. H...


    pub_type: 杂志文章


    authors: Eyler CE,Wu Q,Yan K,MacSwords JM,Chandler-Militello D,Misuraca KL,Lathia JD,Forrester MT,Lee J,Stamler JS,Goldman SA,Bredel M,McLendon RE,Sloan AE,Hjelmeland AB,Rich JN

    更新日期:2011-07-08 00:00:00

  • A deubiquitinating enzyme interacts with SIR4 and regulates silencing in S. cerevisiae.

    abstract::The SIR2, SIR3, and SIR4 proteins are required for silencing of transcription at the silent mating type loci and at telomeres in yeast. Using protein affinity chromatography, we show that SIR2, SIR3, and two proteins of 69 and 110 kDa tightly associate with SIR4. Surprisingly, the 110 kDa SIR4-binding protein is ident...


    pub_type: 杂志文章


    authors: Moazed D,Johnson D

    更新日期:1996-08-23 00:00:00

  • Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly.

    abstract::Kinetochores connect centromeric nucleosomes with mitotic-spindle microtubules through conserved, cross-interacting protein subassemblies. In budding yeast, the heterotetrameric MIND complex (Mtw1, Nnf1, Nsl1, Dsn1), ortholog of the metazoan Mis12 complex, joins the centromere-proximal components, Mif2 and COMA, with ...


    pub_type: 杂志文章


    authors: Dimitrova YN,Jenni S,Valverde R,Khin Y,Harrison SC

    更新日期:2016-11-03 00:00:00

  • TB or Not TB: how Mycobacterium tuberculosis may evade drug treatment.

    abstract::In this issue of Cell, a study by Valerie Mizrahi and her colleagues suggests that a putative error-prone DNA polymerase encoded by the dnaE2 gene of Mycobacterium tuberculosis may bypass certain types of DNA base damage, generating mutations. This may be an important mechanism for generating drug-resistant strains of...


    pub_type: 评论,杂志文章,评审


    authors: Friedberg EC,Fischhaber PL

    更新日期:2003-04-18 00:00:00

  • Saved by the Matrix: UPR Independent Survival under ER Stress.

    abstract::Cells are protected from endoplasmic reticulum stress through the unfolded protein response (UPR). In this issue of Cell, Schinzel, Higuchi-Sanabria, Shalem et al., identify a mechanism that helps cells cope with ER stress but is independent of canonical UPR activation, instead involving the extracellular matrix hyalu...


    pub_type: 评论,杂志文章


    authors: Koopman M,Hetz C,Nollen EAA

    更新日期:2019-11-27 00:00:00

  • Tracing the retrograde route in protein trafficking.

    abstract::Retrograde transport, in which proteins and lipids are shuttled between endosomes and biosynthetic/secretory compartments such as the Golgi apparatus, is crucial for a diverse range of cellular functions. Mechanistic studies that explore the molecular machinery involved in this retrograde trafficking route are sheddin...


    pub_type: 杂志文章,评审


    authors: Johannes L,Popoff V

    更新日期:2008-12-26 00:00:00