Abstract:
:A glycoprotein of molecular weight 130,000 (gP130) has been precipitated from the cytoplasm of GR-strain mouse mammary tumor (GR-MMT) cells by a rabbit antiserum (anti-MMTV) to GR-strain mouse mammary tumor virus (GR-MMTV). This protein was not precipitated by antisera specific for detergent-disrupted C3H-strain MMTV (C3H-MMTV); C3H-MMTV glycoproteins; C3H-MMTV nonglycosylated proteins; GR-MMTV p25 or p12; RIII strain (milk) MMTV proteins; or Rauscher murine leukemia virus (R-MuLV) proteins; nor was it precipitated by normal rabbit serum. Two-dimensional thin layer analysis of 35S-methionine-containing tryptic peptides revealed that five of nine gp33 peptides and one of seven gp55 peptides are shared by gP130 and gPr76env. The envelope protein precursor, gPr76env, contains all of the gp33 peptides and six of seven gp55 peptides. One peptide in gPr76env, possibly a gp55-gp33 junction peptide, is also apparently present in gP130. Six of ten p25 peptides and four more gag-related peptides are shared by PR78gag and gP130. Protein gP130 also contains several tryptic peptides not found in gPr76env or in the core protein precursors Pr78gag, Pr110gag or Pr180gag-pol. Radioimmunoprecipitation experiments showed that gP130 could be precipitated from extracts of GR-MMTV cells with anti-MMTV serum even after antibodies to the known MMTV structural proteins had been removed from the serum by absorption. Both gP130 and a second protein, p30, were found in immunoprecipitates of detergent-disrupted isotopically labeled GR-MMTV treated with the absorbed anti-MMTV serum. These results suggest that antibodies to gP130 in the anti-MMTV serum are capable of recognizing those protein sequences unique to gP130; that is, those protein sequences which are not related to viral structural proteins. In light of these data and data published previously, gP130 is apparently a polyprotein containing juxtaposed components translated from the 5' and 3' end of the MMTV genome and protein components not previously identified as virus-specific.
journal_name
Celljournal_title
Cellauthors
Anderson SJ,Naso RBdoi
10.1016/0092-8674(80)90447-xsubject
Has Abstractpub_date
1980-10-01 00:00:00pages
837-47issue
3eissn
0092-8674issn
1097-4172pii
0092-8674(80)90447-Xjournal_volume
21pub_type
杂志文章相关文献
CELL文献大全abstract::Lipid composition affects the biophysical properties of membranes that provide a platform for receptor-mediated cellular signaling. To study the regulatory role of membrane lipid composition, we combined genetic perturbations of sphingolipid metabolism with the quantification of diverse steps in Toll-like receptor (TL...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.05.051
更新日期:2015-07-02 00:00:00
abstract::The precise control of miR-17∼92 microRNA (miRNA) is essential for normal development, and overexpression of certain miRNAs from this cluster is oncogenic. Here, we find that the relative expression of the six miRNAs processed from the primary (pri-miR-17∼92) transcript is dynamically regulated during embryonic stem c...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.07.008
更新日期:2015-08-13 00:00:00
abstract::Xenopus laevis oocytes respond to high temperature (greater than 31 degrees C) by the synthesis of one major (70 kilodalton) protein and by a gradual reduction in the rate of normal protein synthesis. In contrast with most other cells, the heat-shock response of Xenopus oocytes is controlled exclusively at the transla...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90443-3
更新日期:1982-07-01 00:00:00
abstract::Mutations in the single-minded (sim) gene of Drosophila result in the loss of the precursor cells giving rise to the midline cells of the embryonic central nervous system. We have examined the structure of the sim product by sequencing a sim cDNA clone, and have also determined the subcellular localization of the prot...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(88)90538-7
更新日期:1988-01-15 00:00:00
abstract::DNA fragments containing the silencers that flank the mating type genes at HML alpha are shown to bind specifically to the nuclear scaffold of yeast. The scaffold proteins are solubilized with urea and then renatured to form a soluble extract which allows reconstitution of sequence-specific DNA loops. At the silent ma...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90788-5
更新日期:1989-06-02 00:00:00
abstract::Muscle fibers specialized for fast or slow contraction are arrayed in characteristic patterns within developing limbs. Clones of myoblasts analyzed in vitro express fast and slow myosin isoforms typical of the muscle from which they derive. As a result, it has been suggested that distinct myoblast lineages generate an...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90142-y
更新日期:1992-02-21 00:00:00
abstract::Most inducible transcriptional programs consist of primary and secondary response genes (PRGs and SRGs) that differ in their kinetics of expression and in their requirements for new protein synthesis and chromatin remodeling. Here we show that many PRGs, in contrast to SRGs, have preassembled RNA polymerase II (Pol II...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.05.047
更新日期:2009-07-10 00:00:00
abstract::The switch from short-term to long-term facilitation of the synapses between sensory and motor neurons mediating gill and tail withdrawal reflexes in Aplysia requires CREB-mediated transcription and new protein synthesis. We isolated several downstream genes, one of which encodes a neuron-specific ubiquitin C-terminal...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80188-9
更新日期:1997-04-04 00:00:00
abstract::A complete human gamma 1 heavy chain gene (HIG1) was transferred into mouse cells by protoplast fusion. The HIG1 gene was strongly expressed in mouse myeloma cells but not in mouse fibroblasts (L cells). Nuclear extracts from myeloma cells were injected into L cell transformants containing one copy of the HIG1 gene; t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90534-9
更新日期:1986-04-11 00:00:00
abstract::Pulses of ecdysteroids direct Drosophila through its life cycle by activating stage- and tissue-specific genetic regulatory hierarchies. Here we show that an orphan nuclear receptor, DHR78, functions at the top of the ecdysteroid regulatory hierarchies. Null mutations in DHR78 lead to lethality during the third larval...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81184-8
更新日期:1998-05-15 00:00:00
abstract::The differentiation of body-wall muscle cells was studied in the nematode Caenorhabditis elegans. Specific antibodies to myosin and paramyosin, major protein constituents of differentiated muscle, react with mesodermal cells in wild-type embryos towards the end of the first half of embryogenesis. Immunoreactive cells ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90025-3
更新日期:1982-08-01 00:00:00
abstract::A strong transcription enhancer was identified in the genomic DNA (235 kb) of human cytomegalovirus (HCMV), a ubiquitous and severe pathogen of the herpesvirus group. Cotransfection of enhancerless SV40 DNA with randomly fragmented HCMV DNA yielded two SV40-HCMV recombinant viruses that had incorporated overlapping se...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(85)80025-8
更新日期:1985-06-01 00:00:00
abstract::The majority of the mRNA molecules in HeLa cells contain 1-2 residue(s) of m6Ap and one blocked, methylated 5' terminal "cap" structure. The hnRNA, which is longer than mRNA, contains both m6Ap and caps but 4-6 times as many m6Ap residues per chain. In addition, nuclear molecules contain T2 RNA ase-resistant, methyl-l...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(76)90022-2
更新日期:1976-02-01 00:00:00
abstract::Negative feedback is a widespread feature of signaling pathways. In an unexpected twist described in this issue, He and colleagues identify a membrane-tethered metalloprotease named Tiki that inhibits Wnt signaling by removing an essential eight-residue fragment from Wnt itself. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2012.06.007
更新日期:2012-06-22 00:00:00
abstract::This paper describes a model for the structure of DNA contained in three morphologically similar bacteriophages--T7, P22 and phiCd-1--based on the transient electric dichroism of intact phage. The reduced dichroism of each of the phages at perfect orientation is within the range +0.12 to +0.19. Assuming that the phage...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90026-6
更新日期:1979-03-01 00:00:00
abstract::The cis-trans isomerization of proline serves as a regulatory switch in signaling pathways. We identify the proline isomerase Fpr4, a member of the FK506 binding protein family in Saccharomyces cerevisiae, as an enzyme which binds the amino-terminal tail of histones H3 and H4 and catalyses the isomerization of H3 prol...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.07.026
更新日期:2006-09-08 00:00:00
abstract::Activins are involved in the regulation of multiple biological events, ranging from early development to pituitary function. To characterize the cellular mechanisms involved in these processes, cDNAs coding for an activin receptor were cloned from AtT20 mouse corticotropic cells by screening COS cell transfectants for...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90549-e
更新日期:1991-06-14 00:00:00
abstract::Kinetochores direct attachment of chromosomes to microtubules of the mitotic spindle during cell division. Three recent studies in Cell, including one in this issue, reveal important new roles for two kinetochore protein complexes-Ndc80 and INCENP-Survivin-in establishing the correct attachment of chromosomes to spind...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2006.11.032
更新日期:2006-12-15 00:00:00
abstract::Studies on several recently discovered error-prone DNA polymerases reveal novel structures that may explain the low fidelity of this general class of enzymes, a number of which are involved in the replicative bypass (translesion synthesis) of base damage in DNA. ...
journal_title:Cell
pub_type: 评论,杂志文章,评审
doi:10.1016/s0092-8674(01)00509-8
更新日期:2001-10-05 00:00:00
abstract::Periodic transcription of yeast histone genes has been demonstrated by DNA excess filter hybridization of in vivo pulse-labeled RNA isolated from synchronous cell cultures. Using strains carrying cell division cycle (cdc) mutations, we show that both activation and termination of transcription are determined by tempor...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90036-8
更新日期:1982-08-01 00:00:00
abstract::Mice homozygous for the recessive mutation swaying (sw) are characterized by ataxia and hypertonia, attributed to the malformation of anterior regions of the cerebellum. We show that sw is a deletion of a single base pair from the proto-oncogene Wnt-1. The deletion is predicted to cause premature termination of transl...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90369-a
更新日期:1991-11-29 00:00:00
abstract::The cpd mutation localized by T-DNA tagging on Arabidopsis chromosome 5-14.3 inhibits cell elongation controlled by the ecdysone-like brassinosteroid hormone brassinolide. The cpd mutant displays de-etiolation and derepression of light-induced genes in the dark, as well as dwarfism, male sterility, and activation of s...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81094-6
更新日期:1996-04-19 00:00:00
abstract::We wished to learn whether the genomes of strains of EMB isolated from patients with infectious mononucleosis are consistently distinguishable from those of strains from Burkitt's lymphoma. The genome of a new transforming strains (FF41) of EBV isolated from saliva of a patient with uncomplicated infectious mononucleo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90345-7
更新日期:1981-05-01 00:00:00
abstract::torso encodes a receptor tyrosine kinase (torso) required for anterior and posterior terminal development of the Drosophila embryo. Injecting eggs with in vitro synthesized torso mRNAs revealed that torso activation is governed by an extracellular molecule, presumably the torso ligand, produced at terminal regions of ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90394-r
更新日期:1992-12-11 00:00:00
abstract::A cDNA clone encoding a novel hematopoietic growth factor activity produced by a gibbon T cell line has been identified using a mammalian cell expression cloning system. The sequence of this cDNA proved to have significant homology to the sequence encoding murine interleukin 3 (IL-3). The human gene, which was readily...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90360-0
更新日期:1986-10-10 00:00:00
abstract::Immunofluorescent staining of a pericellular matrix produced by cultured human embryonic skin fibroblasts showed a codistribution among fibronectin, heparan sulfate proteoglycans and part of the chondroitin sulfate in a fibrillar network. Isolated matrix in an "intact" form could be scraped off the dish after detergen...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90221-5
更新日期:1982-03-01 00:00:00
abstract::Dysfunction and loss of insulin-producing pancreatic beta cells represent hallmarks of diabetes mellitus. Here, we show that mice lacking the mitogen-activated protein kinase (MAPK) p38delta display improved glucose tolerance due to enhanced insulin secretion from pancreatic beta cells. Deletion of p38delta results in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2008.11.018
更新日期:2009-01-23 00:00:00
abstract::The amino acid glutamine has a central role in nitrogen metabolism. Although the molecular mechanisms responsible for its transport across cell membranes remain poorly understood, classical amino acid transport system N appears particularly important. Using intracellular pH measurements, we have now identified an orph...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81674-8
更新日期:1999-12-23 00:00:00
abstract::In this issue of Cell, Korber et al. found that a SARS-CoV-2 variant in the spike protein D614G rapidly became dominant around the world. Although clinical and in vitro data suggest that D614G changes the virus phenotype, the impact of the mutation on transmission, disease, and vaccine and therapeutic development are ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2020.06.040
更新日期:2020-08-20 00:00:00
abstract::Nucleosome assembly following DNA replication and gene transcription is important to maintain genome stability and epigenetic information. Newly synthesized histones H3-H4 first bind histone chaperone Asf1 and are then transferred to other chaperones for nucleosome assembly. However, it is unknown how H3-H4 is transfe...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.10.014
更新日期:2013-11-07 00:00:00