Abstract：
:The tyrT locus of E. coli contains a 208 bp spacer region that separates two copies of sequence encoding tRNATyr1. The spacer includes a 120 bp sequence that is homologous to a sequence that is repeated three times in the distal portion of the tyrT locus. The tyrT locus possesses a graded set of transcription termination sites that are spaced at 180 base intervals, corresponding to the distal repeated gene structure. The major termination site occurs within the second repeat unit, 225 bases beyond the mature tRNA sequences. In the presence of a temperature-sensitive rho protein there is increased read-through at this site to a termination site located 180 bases downstream in the third repeat and to several termination sites even further downstream. The primary native transcript, in the region distal to the second tRNA, carries the information for a low molecular weight, extremely basic protein. Although analogous coding sequences are present in the spacer and other repeat units, because of single base substitutions these sequences are pseudogenes. The parallel between the tyrT and TyrU gene clusters is discussed in relation to dual function transcripts that specify both tRNA and protein.
journal_name
Celljournal_title
Cellauthors
Rossi J,Egan J,Hudson L,Landy Adoi
10.1016/0092-8674(81)90199-9subject
Has Abstractpub_date
1981-11-01 00:00:00pages
305-14issue
3 Pt 1eissn
0092-8674issn
1097-4172pii
0092-8674(81)90199-9journal_volume
26pub_type
杂志文章相关文献
CELL文献大全abstract：:Chemical modifications of histones and DNA, such as histone methylation, histone acetylation, and DNA methylation, play critical roles in epigenetic gene regulation. Many of the enzymes that add or remove such chemical modifications are known, or might be suspected, to be sensitive to changes in intracellular metaboli...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2013.03.004
更新日期：2013-03-28 00:00:00
abstract：:Histone acetyltransferases (HATs) and histone deacetylases (HDACs) conduct many critical functions through nonhistone substrates in metazoans, but only chromatin-associated nonhistone substrates are known in Saccharomyces cerevisiae. Using yeast proteome microarrays, we identified and validated many nonchromatin subst...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2009.01.033
更新日期：2009-03-20 00:00:00
abstract：:A novel trimolecular mechanism of microbial attachment to mammalian host cells was characterized for the obligate intracellular pathogen Chlamydia trachomatis. Using purified glycosaminoglycans (GAGs) and specific GAG lyases, we demonstrated that a heparan sulfate-like GAG present on the surface of chlamydia organisms...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90296-o
更新日期：1992-05-29 00:00:00
abstract：:Multidrug resistance in human cells results from increased expression of the mdr1 (P-glycoprotein) gene. Although the same gene is activated in cells selected with different drugs, multidrug-resistant cell lines can be preferentially resistant to their selecting agent. The mdr1 cDNA sequence from vinblastine-selected ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(88)90568-5
更新日期：1988-05-20 00:00:00
abstract：:Developing cells of the cellular slime mold Dictyostelium discoideum release a low molecular weight metabolite (Slug Turning Factor, STF) which, at high uniform concentrations, interferes with phototaxis and thermotaxis by D. discoideum slugs. D. discoideum slugs migrating in darkness are repelled by (exhibit negative...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90444-x
更新日期：1981-03-01 00:00:00
abstract：:Anterograde transport in the early secretory pathway is mediated by COPII-coated vesicles. Stagg et al. (2008) have now visualized the double-layered COPII coat using electron cryomicroscopy, providing insight into how coats are assembled to accommodate cargo of different sizes. ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2008.07.027
更新日期：2008-08-08 00:00:00
abstract：:Overlapping cDNA clones from the translocation breakpoint region (TBR) gene, recently discovered at the neurofibromatosis type 1 locus and found to be interrupted by deletions and a t(17;22) translocation, have been sequenced. A 4 kb sequence of the transcript of the TBR gene has been compared with sequences of genomi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90253-b
更新日期：1990-07-13 00:00:00
abstract：:At anaphase onset, the protease separase triggers chromosome segregation by cleaving the chromosomal cohesin complex. Here, we show that cohesin destruction in metaphase is sufficient for segregation of much of the budding yeast genome, but not of the long arm of chromosome XII that contains the rDNA repeats. rDNA in ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(04)00415-5
更新日期：2004-05-14 00:00:00
abstract：:The mutated gene responsible for the tubby obesity phenotype has been identified by positional cloning. A single base change within a splice donor site results in the incorrect retention of a single intron in the mature tub mRNA transcript. The consequence of this mutation is the substitution of the carboxy-terminal 4...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81104-6
更新日期：1996-04-19 00:00:00
abstract：:Clinical trials are key to translating scientific advances into progress in cancer research and care. Confronted by developments in basic science, the landscape of clinical cancer research is rapidly evolving. Here, we review recent changes in clinical trials' design and conduct, and we forecast future directions towa...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.01.027
更新日期：2017-02-09 00:00:00
abstract：:Abiotic stress, such as salinity, drought, and cold, causes detrimental yield losses for all major plant crop species. Understanding mechanisms that improve plants' ability to produce biomass, which largely is constituted by the plant cell wall, is therefore of upmost importance for agricultural activities. Cellulose ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.08.028
更新日期：2015-09-10 00:00:00
abstract：:Chromosomal translocations between antigen receptor loci and oncogenes are a hallmark of lymphoid cancers. Several new studies now reveal that programmed DNA breaks created during assembly of antigen receptor genes can be channeled into an alternative DNA end-joining pathway that is implicated in the chromosomal trans...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2007.10.005
更新日期：2007-10-19 00:00:00
abstract：:The elucidation of the genetic code remains among the most influential discoveries in biology. While innumerable studies have validated the general universality of the code and its value in predicting and analyzing protein coding sequences, established and emerging work has also suggested that full genome decryption m...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2016.09.022
更新日期：2016-12-15 00:00:00
abstract：:To mechanistically characterize the microevolutionary processes active in altering transcription factor (TF) binding among closely related mammals, we compared the genome-wide binding of three tissue-specific TFs that control liver gene expression in six rodents. Despite an overall fast turnover of TF binding location...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.07.007
更新日期：2013-08-01 00:00:00
abstract：:Salmonella possesses several flagella, each capable of counterclockwise and clockwise rotation. Counterclockwise rotation produces swimming, clockwise rotation produces tumbling. Switching between senses occurs stochastically. The rotational sense of individual flagella on a single cell could be monitored under specia...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90501-9
更新日期：1983-01-01 00:00:00
abstract：:Lipid composition affects the biophysical properties of membranes that provide a platform for receptor-mediated cellular signaling. To study the regulatory role of membrane lipid composition, we combined genetic perturbations of sphingolipid metabolism with the quantification of diverse steps in Toll-like receptor (TL...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.05.051
更新日期：2015-07-02 00:00:00
abstract：:We show that transfer of human fibroblast chromosome 11 (containing the human MyoD gene) from primary cells into 10T1/2 mouse fibroblasts by microcell fusion activates expression of the transferred human MyoD gene and converts these cells to myoblasts. Transfer of human chromosome 11 into B78 melanoma cells also leads...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90285-m
更新日期：1990-10-05 00:00:00
abstract：:Dysfunction and loss of insulin-producing pancreatic beta cells represent hallmarks of diabetes mellitus. Here, we show that mice lacking the mitogen-activated protein kinase (MAPK) p38delta display improved glucose tolerance due to enhanced insulin secretion from pancreatic beta cells. Deletion of p38delta results in...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2008.11.018
更新日期：2009-01-23 00:00:00
abstract：:Mouse monoclonal antibody 4-I-244 detects a developmentally regulated antigen in embryos of Volvox carteri and inhibits specifically the morphogenetic process of inversion (the process by which the embryo turns inside out). Antigen 4-I-244 was chemically characterized as a complex phytosphingolipid containing the neut...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(86)90889-5
更新日期：1986-08-15 00:00:00
abstract：:The mechanisms of transcriptional activation in heterochromatin were investigated by using FISH to directly visualize changes in chromatin organization during activation of a heterochromatic lambda5 transgene. A DNase I hypersensitive site was shown to relocate the transgene to the outside of the pericentromeric heter...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)00177-x
更新日期：2000-11-22 00:00:00
abstract：:We used the interaction trap, a yeast genetic selection for interacting proteins, to isolate human cyclin-dependent kinase interactor 1 (Cdi1). In yeast, Cdi1 interacts with cyclin-dependent kinases, including human Cdc2, Cdk2, and Cdk3, but not with Ckd4. In HeLa cells, Cdi1 is expressed at the G1 to S transition, an...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(93)90498-f
更新日期：1993-11-19 00:00:00
abstract：:The rps12 gene codes for chloroplast ribosomal protein S12. In the tobacco chloroplast genome (156 kbp circular DNA), exons II and III of this gene are separated by an intron of 536 bases and are present in two copies in the inverted repeat region, while exon I is located in the large single copy region at a distance ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(87)90361-8
更新日期：1987-01-16 00:00:00
abstract：:ATRX is an X-linked gene of the SWI/SNF family, mutations in which cause syndromal mental retardation and downregulation of α-globin expression. Here we show that ATRX binds to tandem repeat (TR) sequences in both telomeres and euchromatin. Genes associated with these TRs can be dysregulated when ATRX is mutated, and ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2010.09.023
更新日期：2010-10-29 00:00:00
abstract：:DNA carrying inverted repeats was tested for transposition within the Drosophila genome. Five Bam HI segments containing related inverted repeats were isolated from D. melanogaster and analyzed by electron microscopy and restriction mapping. Southern blot experiments using single-copy flanking sequences as probes allo...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(80)90310-4
更新日期：1980-07-01 00:00:00
abstract：:Nucleosomes help structure chromosomes by compacting DNA into fibers. To gain insight into how nucleosomes are arranged in vivo, we combined quantitative super-resolution nanoscopy with computer simulations to visualize and count nucleosomes along the chromatin fiber in single nuclei. Nucleosomes assembled in heteroge...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.01.054
更新日期：2015-03-12 00:00:00
abstract：:The relationship between activation of resting chick embryo fibroblasts by proteases and proteolytic alteration of the cell surface has been investigated. Five different proteases were examined: trypsin, collagenase, plasmin, alpha-chymotrypsin, and thrombin. All of these proteases, when added to the culture medium at...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(75)90004-5
更新日期：1975-10-01 00:00:00
abstract：:Cell surface complexes of class I MHC molecules and bound peptide antigens serve as specific recognition elements controlling the cytotoxic immune response. The 2.1 A structure of the human class I MHC molecule HLA-B27 provides a detailed composite image of a co-crystallized collection of HLA-B27-bound peptides, indic...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90252-8
更新日期：1992-09-18 00:00:00
abstract：:Phagosomes were traditionally thought to originate from an invagination and scission of the plasma membrane to form a distinct intracellular vacuole. An alternative model implicating the endoplasmic reticulum (ER) as a major component of nascent and maturing phagosomes was recently proposed (Gagnon et al., 2002). To r...
journal_title：Cell
pub_type： 信件
doi：10.1016/j.cell.2005.08.018
更新日期：2005-10-07 00:00:00
abstract：:Mice deficient for interleukin-2 develop normally during the first 3-4 weeks of age. However, later on they become severely compromised, and about 50% of the animals die between 4 and 9 weeks after birth. Of the remaining mice, 100% develop an inflammatory bowel disease with striking clinical and histological similari...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(93)80067-o
更新日期：1993-10-22 00:00:00
abstract：:We describe here a bizarre organization of the single large subunit (LSU) and small subunit (SSU) rRNA genes in the mitochondrial DNA of Chlamydomonas reinhardtii. Each gene is discontinuous, with gene pieces encoding specific rRNA domains ("modules") interspersed with one another and with intact protein coding and tR...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(88)90026-8
更新日期：1988-11-04 00:00:00
