Direct Visualization of Wide Fusion-Fission Pores and Their Highly Varied Dynamics.

abstract：:Fractionation of HeLa cell extracts reveals the presence of a promoter-specific transcription factor, Sp 1, which activates a class of promoters that includes the SV40 early promoter but not several others that have been tested. We analyzed SV40 early-promoter deletion mutants and determined that transcriptional activ...

journal_title：Cell

authors： Dynan WS,Tjian R

更新日期：1983-11-01 00:00:00

abstract：:The cytosolic protein APAF1, human homolog of C. elegans CED-4, participates in the CASPASE 9 (CASP9)-dependent activation of CASP3 in the general apoptotic pathway. We have generated by gene trap a null allele of the murine Apaf1. Homozygous mutants die at embryonic day 16.5. Their phenotype includes severe craniofac...

journal_title：Cell

authors： Cecconi F,Alvarez-Bolado G,Meyer BI,Roth KA,Gruss P

更新日期：1998-09-18 00:00:00

abstract：:We have characterized a nondifferentiating mouse muscle cell line, NFB, that represses the activity of the helix-loop-helix (HLH) family of myogenic regulators, yet expresses sarcomeric actins. The NFB MyoD gene is silent, but can be activated upon transfection of a long terminal region-controlled chicken MyoD cDNA, r...

journal_title：Cell

authors： Peterson CA,Gordon H,Hall ZW,Paterson BM,Blau HM

更新日期：1990-08-10 00:00:00

abstract：:Rare diseases are powerful windows into biological processes and can serve as models for the development of therapeutic strategies. The progress made on the premature aging disorder Progeria is a shining example of the impact that studies of rare diseases can have. ...

journal_title：Cell

authors： Gordon LB,Rothman FG,López-Otín C,Misteli T

更新日期：2014-01-30 00:00:00

abstract：:Living organisms sense their physical environment through cellular mechanotransduction, which converts mechanical forces into electrical and biochemical signals. In turn, signal transduction serves a wide variety of functions, from basic cellular processes as diverse as proliferation, differentiation, migration, and a...

journal_title：Cell

authors： Delmas P,Coste B

更新日期：2013-10-10 00:00:00

abstract：:We recently described the purification and cloning of extracellular signal-regulated kinase 1 (ERK1), which appears to play a pivotal role in converting tyrosine phosphorylation into the serine/threonine phosphorylations that regulate downstream events. We now describe cloning and characterization of two ERK1-related ...

journal_title：Cell

authors： Boulton TG,Nye SH,Robbins DJ,Ip NY,Radziejewska E,Morgenbesser SD,DePinho RA,Panayotatos N,Cobb MH,Yancopoulos GD

更新日期：1991-05-17 00:00:00

abstract：:We examined the interactions of lipid vesicles (liposomes) labeled with various fluorescent markers with the intracellular membranes of semi-intact ("perforated") fibroblasts. When incubations were performed in the presence of an ATP-regenerating system, both vesicle lipids and entrapped water soluble markers were tra...

journal_title：Cell

authors： Kobayashi T,Pagano RE

更新日期：1988-12-02 00:00:00

abstract：:In the SV40-transformed mouse embryo fibroblast cell line SVT2/S, genomic rearrangements involving the SV40 DNA and flanking host sequences were identified by Southern blot hybridization using viral DNA as probe. No rearrangements of SV40 DNA integrated into nonpermissive mouse cells have been previously described. Th...

journal_title：Cell

authors： Sager R,Anisowicz A,Howell N

更新日期：1981-01-01 00:00:00

abstract：:Four major receptor families enable cells to respond to chemical and physical signals from their proximal environment. The ligand- and voltage-gated ion channels, G-protein-coupled receptors, nuclear hormone receptors, and receptor tyrosine kinases are all allosteric proteins that carry multiple, spatially distinct, y...

journal_title：Cell

authors： Changeux JP,Christopoulos A

更新日期：2016-08-25 00:00:00

abstract：:Replication of Escherichia coli plasmid p15A was examined by use of a cell extract or a mixture of three purified E. coli enzymes: RNA polymerase; RNAase H; and DNA polymerase I. In each system, replication initiates at any of three consecutive nucleotides located at a unique site. Primer transcription starts 508 bp u...

journal_title：Cell

authors： Selzer G,Som T,Itoh T,Tomizawa J

更新日期：1983-01-01 00:00:00

abstract：:Immune responses in diverse tissue sites are critical for protective immunity and homeostasis. Here, we investigate how tissue localization regulates the development and function of human natural killer (NK) cells, innate lymphocytes important for anti-viral and tumor immunity. Integrating high-dimensional analysis of...

journal_title：Cell

authors： Dogra P,Rancan C,Ma W,Toth M,Senda T,Carpenter DJ,Kubota M,Matsumoto R,Thapa P,Szabo PA,Li Poon MM,Li J,Arakawa-Hoyt J,Shen Y,Fong L,Lanier LL,Farber DL

更新日期：2020-02-20 00:00:00

abstract：:Competence development and sporulation in B. subtilis are partly controlled by peptides that accumulate in culture medium as cells grow to high density. We constructed two genes that encode mature forms of two different signaling molecules, the PhrA peptide that stimulates sporulation, and CSF, the competence- and spo...

journal_title：Cell

authors： Lazazzera BA,Solomon JM,Grossman AD

更新日期：1997-06-13 00:00:00

abstract：:The initiation of transcription from the nitrogen-regulated promoter glnAp2 requires RNA polymerase containing sigma 54, the transcriptional activator NRI, and the protein kinase NRII, responsible for the conversion of NRI to the active NRI-phosphate. NRI-phosphate does not increase the ability of sigma 54-containing ...

journal_title：Cell

authors： Ninfa AJ,Reitzer LJ,Magasanik B

更新日期：1987-09-25 00:00:00

abstract：:Advances in genomic technology have produced an explosion of new information about the genetic basis for human disease, fueling extraordinarily high expectations for improved treatments. This perspective will take brief stock of what genetics/genomics have brought to clinical practice to date and what we might expect ...

journal_title：Cell

authors： Ginsburg D

更新日期：2011-09-30 00:00:00

abstract：:Methylation of deoxycytidine incorporated by DNA excision-repair was studied in human diploid fibroblasts following damage with ultraviolet radiation, N-methyl-N-nitrosourea, or N-acetoxy-2-acetylaminofluorene. In confluent, nondividing cells, methylation in repair patches induced by all three agents is slow and incom...

journal_title：Cell

authors： Kastan MB,Gowans BJ,Lieberman MW

更新日期：1982-09-01 00:00:00

abstract：:Developmental signaling pathways needed to evolve to be robust against environmental fluctuations. In this issue, Shimizu et al. reveal a complex system of interacting endocytic pathways that help to maintain consistent levels of Notch activity across a range of temperatures. ...

journal_title：Cell

authors： Delidakis C

更新日期：2014-05-22 00:00:00

abstract：:Mechanistic understanding of pre-mRNA splicing requires detailed structural information on various states of the spliceosome. Here we report the cryo electron microscopy (cryo-EM) structure of the human spliceosome just before exon ligation (the C∗ complex) at an average resolution of 3.76 Å. The splicing factor Prp17...

journal_title：Cell

authors： Zhang X,Yan C,Hang J,Finci LI,Lei J,Shi Y

更新日期：2017-05-18 00:00:00

abstract：:From the approximately 1,200 odorant receptor (OR) genes in the mouse genome, an olfactory sensory neuron is thought to express only one gene. The mechanisms of OR gene choice are not understood. A 2.1 kilobase region (the H element) adjacent to a cluster of seven OR genes has been proposed as a trans- and pan-enhance...

journal_title：Cell

authors： Fuss SH,Omura M,Mombaerts P

更新日期：2007-07-27 00:00:00

abstract：:The light-sensing organelle of the vertebrate rod photoreceptor, the outer segment (OS), is a modified cilium containing approximately 1,000 stacked disc membranes that are densely packed with visual pigment rhodopsin. The mammalian OS is renewed every ten days; new discs are assembled at the base of the OS by a poorl...

journal_title：Cell

authors： Chuang JZ,Zhao Y,Sung CH

更新日期：2007-08-10 00:00:00

abstract：:We have shown that the DNA sequences adjacent to the telomeres of Saccharomyces cerevisiae chromosomes are highly conserved and contain a high density of replication origins. The salient features of these telomeres can be summarized as follows. There are three moderately repetitive elements present at the telomeres: t...

journal_title：Cell

authors： Chan CS,Tye BK

更新日期：1983-06-01 00:00:00

abstract：:We have isolated two adenylyl cyclase genes, designated ACA and ACG, from Dictyostelium. The proposed structure for ACA resembles that proposed for mammalian adenylyl cyclases: two large hydrophilic domains and two sets of six transmembrane spans. ACG has a novel structure, reminiscent of the membrane-bound guanylyl c...

journal_title：Cell

authors： Pitt GS,Milona N,Borleis J,Lin KC,Reed RR,Devreotes PN

更新日期：1992-04-17 00:00:00

abstract：:Human hepatitis B virus core protein expressed in E. coli assembles into two sizes of particle. We have determined their three-dimensional structures by electron cryomicroscopy and image processing. The large and small particles correspond to triangulation number T = 4 and T = 3 dimer clustered packings, containing 24...

journal_title：Cell

authors： Crowther RA,Kiselev NA,Böttcher B,Berriman JA,Borisova GP,Ose V,Pumpens P

更新日期：1994-06-17 00:00:00

abstract：:Recent studies are beginning to shed light on the mechanism of ribosome-catalyzed peptide bond formation. ...

journal_title：Cell

authors： Green R,Lorsch JR

更新日期：2002-09-20 00:00:00

abstract：:We have examined the molecular basis of the response of individuals of D. melanogaster to artificial selection for high and low abdominal bristles. By monitoring the fate of particular rDNA spacer length variants associated with individually isolated X and Y chromosomes, we show that flies from the low bristle number ...

journal_title：Cell

authors： Coen ES,Dover GA

更新日期：1983-07-01 00:00:00

abstract：:The sites at which topoisomerase I interacts with the transcriptionally active ribosomal chromatin of Xenopus oocytes were mapped by treating oocyte nuclei first with camptothecin to stabilize topoisomerase I-rDNA reaction intermediates, and then with SDS to resolve these adducts as protein-linked nicks in the DNA. St...

journal_title：Cell

authors： Culotta V,Sollner-Webb B

更新日期：1988-02-26 00:00:00

abstract：:Clec16a has been identified as a disease susceptibility gene for type 1 diabetes, multiple sclerosis, and adrenal dysfunction, but its function is unknown. Here we report that Clec16a is a membrane-associated endosomal protein that interacts with E3 ubiquitin ligase Nrdp1. Loss of Clec16a leads to an increase in the N...

journal_title：Cell

authors： Soleimanpour SA,Gupta A,Bakay M,Ferrari AM,Groff DN,Fadista J,Spruce LA,Kushner JA,Groop L,Seeholzer SH,Kaufman BA,Hakonarson H,Stoffers DA

更新日期：2014-06-19 00:00:00

abstract：:Potassium channels embedded in cell membranes employ gates to regulate K+ current. While a specific constriction in the permeation pathway has historically been implicated in gating, recent reports suggest that the signature ion selectivity filter located in the outer membrane leaflet may be equally important. Inwardl...

journal_title：Cell

authors： Clarke OB,Caputo AT,Hill AP,Vandenberg JI,Smith BJ,Gulbis JM

更新日期：2010-06-11 00:00:00

abstract：:The science advisor to the US president has the opportunity to influence how scientific research is supported by the federal government, as well as the ways in which science and technology can be applied to meet the needs of society. ...

journal_title：Cell

authors： Lane NF,Matthews KR

更新日期：2009-11-25 00:00:00

abstract：:The X-linked Foxp3 is a member of the forkhead/winged helix transcription factor family. Germline mutations cause lethal autoimmune diseases in males. Serendipitously, we observed that female mice heterozygous for the "scurfin" mutation of the Foxp3 gene (Foxp3(sf/+)) developed cancer at a high rate. The majority of t...

journal_title：Cell

authors： Zuo T,Wang L,Morrison C,Chang X,Zhang H,Li W,Liu Y,Wang Y,Liu X,Chan MW,Liu JQ,Love R,Liu CG,Godfrey V,Shen R,Huang TH,Yang T,Park BK,Wang CY,Zheng P,Liu Y

更新日期：2007-06-29 00:00:00

abstract：:The quest to slow aging has come far, and what used to be the domain of science fiction writers and snake oil salesmen may soon become science fact. Innovative new approaches, such as the use of the very short-lived African killifish (Harel et al.), are bridging the translational gap and bring the promise of healthy l...

journal_title：Cell

authors： Wang AM,Promislow DEL,Kaeberlein M

更新日期：2015-02-26 00:00:00