Abstract:
:Rodent cells resistant to PALA, a specific inhibitor of the aspartate transcarbamylase activity of the multifunctional CAD protein, overproduce CAD as a result of amplification of the CAD gene. We cloned a functional CAD gene from Syrian hamster cells using a cosmid vector. Two independently isolated cosmids containing CAD genes have inserts 40 and 45 kb long. We introduced the cloned genes into CAD-deficient Chinese hamster ovary (CHO) cell mutants by fusing them with protoplasts of Escherichia coli containing the cosmids. We also introduced the cloned genes into wild-type CHO cells by selecting cells that became resistant to high concentrations of PALA following protoplast fusion. The transformants of the mutant and wild-type CHO cells contain multiple active copies of the donated Syrian hamster CAD genes. The cloned genes in three independent transformants are integrated into host-cell chromosomes at single locations identified by in situ hybridization. In two of these transformants, the genes are located in one X chromosome or in a chromosome resembling the X. In the third case, the genes are located in a small metacentric or rearranged chromosome.
journal_name
Celljournal_title
Cellauthors
de Saint Vincent BR,Delbrück S,Eckhart W,Meinkoth J,Vitto L,Wahl Gdoi
10.1016/0092-8674(81)90410-4subject
Has Abstractpub_date
1981-12-01 00:00:00pages
267-77issue
2 Pt 1eissn
0092-8674issn
1097-4172pii
0092-8674(81)90410-4journal_volume
27pub_type
杂志文章相关文献
CELL文献大全abstract::Mechanistic understanding of pre-mRNA splicing requires detailed structural information on various states of the spliceosome. Here we report the cryo electron microscopy (cryo-EM) structure of the human spliceosome just before exon ligation (the C∗ complex) at an average resolution of 3.76 Å. The splicing factor Prp17...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.04.033
更新日期:2017-05-18 00:00:00
abstract::Drosophila Reaper (RPR), Head Involution Defective (HID), and GRIM induce caspase-dependent cell death and physically interact with the cell death inhibitor DIAP1. Here we show that HID blocks DIAP1's ability to inhibit caspase activity and provide evidence suggesting that RPR and GRIM can act similarly. Based on thes...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81974-1
更新日期:1999-08-20 00:00:00
abstract::We have determined the nucleotide sequence of the transposable maize controlling element Activator (Ac). The Ac element is 4563 bp long and has an imperfect terminal repetition of 11 bp. The element contains two open reading frames (ORF) encoding polypeptides of 839 and 210 amino acids. Evidence derived from structura...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90395-7
更新日期:1984-06-01 00:00:00
abstract::Many naturalistic behaviors are built from modular components that are expressed sequentially. Although striatal circuits have been implicated in action selection and implementation, the neural mechanisms that compose behavior in unrestrained animals are not well understood. Here, we record bulk and cellular neural ac...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.04.019
更新日期:2018-06-28 00:00:00
abstract::Hematopoietic stem cells (HSCs) exist in the bone marrow and circulate in the blood. In this issue, Massberg et al. (2007) report that HSCs also travel through the lymphatic system. Furthermore, migration of HSCs--which express Toll-like receptors--allows the recognition of pathogenic molecules in peripheral tissues t...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2007.11.010
更新日期:2007-11-30 00:00:00
abstract::The classical model for the activation of the nucleotide exchange factor Son of sevenless (SOS) involves its recruitment to the membrane, where it engages Ras. The recent discovery that Ras*GTP is an allosteric activator of SOS indicated that the regulation of SOS is more complex than originally envisaged. We now pres...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2004.10.005
更新日期:2004-10-29 00:00:00
abstract::The crystal structure of the β2-adrenergic receptor (β2AR) bound to the G protein adenylyl cyclase stimulatory G protein (Gs) captured the complex in a nucleotide-free state (β2AR-Gsempty). Unfortunately, the β2AR-Gsempty complex does not provide a clear explanation for G protein coupling specificity. Evidence from se...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.04.021
更新日期:2019-05-16 00:00:00
abstract::Evasion of DNA damage-induced cell death, via mutation of the p53 tumor suppressor or overexpression of prosurvival Bcl-2 family proteins, is a key step toward malignant transformation and therapeutic resistance. We report that depletion or acute inhibition of checkpoint kinase 1 (Chk1) is sufficient to restore gamma-...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2008.03.037
更新日期:2008-05-30 00:00:00
abstract::The basal ganglia (BG) are critical for adaptive motor control, but the circuit principles underlying their pathway-specific modulation of target regions are not well understood. Here, we dissect the mechanisms underlying BG direct and indirect pathway-mediated control of the mesencephalic locomotor region (MLR), a br...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.12.037
更新日期:2016-01-28 00:00:00
abstract::Mammalian models of longevity are related primarily to caloric restriction and alterations in metabolism. We examined mice in which type 5 adenylyl cyclase (AC5) is knocked out (AC5 KO) and which are resistant to cardiac stress and have increased median lifespan of approximately 30%. AC5 KO mice are protected from red...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.05.038
更新日期:2007-07-27 00:00:00
abstract::The Xist gene maps to the X inactivation center region in both mouse and human, and previous analysis of the 3' end of the gene has demonstrated inactive X-specific expression, suggesting a possible role in X inactivation. We have now analyzed the entire mouse Xist gene. The mature inactive X-specific transcript is 15...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90519-i
更新日期:1992-10-30 00:00:00
abstract::The transcription factor NRF2 is a master regulator of the cellular antioxidant response, and it is often genetically activated in non-small-cell lung cancers (NSCLCs) by, for instance, mutations in the negative regulator KEAP1. While direct pharmacological inhibition of NRF2 has proven challenging, its aberrant activ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.08.051
更新日期:2017-10-19 00:00:00
abstract::Trigger factor (TF) is a molecular chaperone that binds to bacterial ribosomes where it contacts emerging nascent chains, but TF is also abundant free in the cytosol where its activity is less well characterized. In vitro studies show that TF promotes protein refolding. We find here that ribosome-free TF stably associ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.07.044
更新日期:2009-09-04 00:00:00
abstract::Cell-cell junctions respond to mechanical forces by changing their organization and function. To gain insight into the mechanochemical basis underlying junction mechanosensitivity, we analyzed tight junction (TJ) formation between the enveloping cell layer (EVL) and the yolk syncytial layer (YSL) in the gastrulating z...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.10.006
更新日期:2019-10-31 00:00:00
abstract::How difficult is to go from egg to implanted embryo? The evolution of placentation in eutherian mammals created enormous challenges, in particular to the maternal immune system, as the fetus expresses paternal antigens that are capable of triggering immune rejection. Samstein et al. reveal a role for inducible regulat...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2012.06.020
更新日期:2012-07-06 00:00:00
abstract::Competence development and sporulation in B. subtilis are partly controlled by peptides that accumulate in culture medium as cells grow to high density. We constructed two genes that encode mature forms of two different signaling molecules, the PhrA peptide that stimulates sporulation, and CSF, the competence- and spo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80277-9
更新日期:1997-06-13 00:00:00
abstract::Telomere capping is the essential function of telomeres. To identify new genes involved in telomere capping, we carried out a genome-wide screen in Saccharomyces cerevisiae for suppressors of cdc13-1, an allele of the telomere-capping protein Cdc13. We report the identification of five novel suppressors, including the...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2005.12.044
更新日期:2006-03-24 00:00:00
abstract::Members of the myocyte enhancer factor-2 (MEF2) family of MADS domain transcription factors cannot induce myogenesis in transfected fibroblasts, but when coexpressed with the myogenic basic-helix-loop-helix (bHLH) proteins MyoD or myogenin they dramatically increase the extent of myogenic conversion above that seen wi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90139-6
更新日期:1995-12-29 00:00:00
abstract::We have identified a 2.4 kb partial cDNA clone (pDL34) for human von Willebrand factor (vWf) mRNA. pDL34 was selected by screening an endothelial cell cDNA library with a radiolabeled reverse transcript of mRNA obtained by specific immunoisolation of vWf polysomes from endothelial cells. pDL34 selectively hybridized t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90060-1
更新日期:1985-05-01 00:00:00
abstract::We constructed rabbit beta-globin genes with deletions in the large intron, extending from the midpoint toward the 5' or 3' splice sites. Analysis of transcripts in transformed HeLa cells showed that six 5' proximal intron nucleotides allowed normal splicing. Correct splicing at the 3' splice site required 12 or more ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90426-4
更新日期:1984-07-01 00:00:00
abstract::Portions of the nascent chain are exposed to the lumen, the cytosol, or neither at different stages during the cotranslational integration of a protein into the ER membrane, as shown by compartment-specific collisional quenching of fluorophores incorporated into the polypeptide. The opening or closing of each end of t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80311-6
更新日期:1997-07-11 00:00:00
abstract::Toxins have evolved to target regions of membrane ion channels that underlie ligand binding, gating, or ion permeation, and have thus served as invaluable tools for probing channel structure and function. Here, we describe a peptide toxin from the Earth Tiger tarantula that selectively and irreversibly activates the c...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2010.03.052
更新日期:2010-05-28 00:00:00
abstract::Exit from mitosis must not occur prior to partitioning of chromosomes between daughter cells. We find that the GTP binding protein Tem1, a regulator of mitotic exit, is present on the spindle pole body that migrates into the bud during S phase and mitosis. Tem1's exchange factor, Lte1, localizes to the bud. Thus, Tem1...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)00007-6
更新日期:2000-07-07 00:00:00
abstract::Poorly immunogenic tumor cells evade host immunity and grow even in the presence of an intact immune system, but the complex mechanisms regulating tumor immunogenicity have not been elucidated. Here, we discovered an unexpected role of the Hippo pathway in suppressing anti-tumor immunity. We demonstrate that, in three...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.11.005
更新日期:2016-12-01 00:00:00
abstract::Staphylococcal enterotoxin B is known to be a powerful T cell stimulant in mouse and man. In this paper we show that, for mice, this is because the protein in association with major histocompatibility complex class II molecules stimulates virtually all T cells bearing V beta 3 and V beta 8.1, 8.2, and 8.3, and few oth...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90980-x
更新日期:1989-01-13 00:00:00
abstract::The rabbit alpha-globin DNA insertion in the chimeric plasmid pHb 72 (Liu et al., 1977) has been sequenced by the method of Maxam and Gilbert (1977). This has enabled us to determine the messenger RNA(mRNA) sequence beginning in the 5' untranslated region 9 nucleotides before the initiation codon and extending through...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90081-8
更新日期:1978-09-01 00:00:00
abstract::Pancreatic cancer is one of the most lethal malignancies due to its late diagnosis and limited response to treatment. Tractable methods to identify and interrogate pathways involved in pancreatic tumorigenesis are urgently needed. We established organoid models from normal and neoplastic murine and human pancreas tiss...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.12.021
更新日期:2015-01-15 00:00:00
abstract::A diverse range of ultimate chemical carcinogens inhibited the transfer of methyl groups from S-adenosylmethionine to hemimethylated DNA in a reaction catalyzed by mouse spleen methyltransferase. The formation of alkali-labile sites in DNA lessened its ability to accept methyl groups in vitro, but the methylation reac...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90514-7
更新日期:1983-01-01 00:00:00
abstract::Erythropoietin (EPO) production in the kidney is regulated by the oxygen-sensing transcription factor HIF-1α, which is degraded under normoxic conditions by HIF-prolyl hydroxylase (HIF-PHD). Inhibition of HIF-PHD by roxadustat leads to increased EPO production, better iron absorption, and amelioration of anemia in chr...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.12.010
更新日期:2020-01-09 00:00:00
abstract::Plants greatly rely on their root microbiome for uptake of nutrients and protection against stresses. Recent studies have uncovered the involvement of plant stress responses in the assembly of plant-beneficial microbiomes. To facilitate durable crop production, deciphering the driving forces that shape the microbiome ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.02.024
更新日期:2018-03-08 00:00:00